Q96YL5 (NADE_SULTO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	STK_21590

Organism

Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) [Complete proteome] [HAMAP]

Taxonomic identifier

273063 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 279	279	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_0000152236
Regions
	Nucleotide binding	40 – 47	8	ATP By similarity
Sites
	Active site	42	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q96YL5 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: BA0B7CC3DE7D4356	FASTA	279	31,822
	10 20 30 40 50 60 MMPEYVRKKL TLDFGQVTDY IVRRIREYIE ESKKEGGIIG LSGGIDSSVT TILLSRATNN 70 80 90 100 110 120 FYILLMPTSS TPQKDIEDAM KIIKIVNGEN KYSYINIDEI INEFSSIVNI SDKIVIGNIK 130 140 150 160 170 180 ARVRMTLLYA FAQKMNYLVI GTGDKSEIML GYFTKYGDGG VDVLPIGDLY KTQVRMLGNY 190 200 210 220 230 240 LGVPEEIVKK PPSPALWEGQ TAEGEIGLDY ETIDSILYLK FEEMREPEEI AEMTKTSYDK 250 260 270 VIKIINMVKT SQHKRLPPEI FRLSGRAINS DWRYPRQWG

References

[1]

"Complete genome sequence of an aerobic thermoacidophilic Crenarchaeon, Sulfolobus tokodaii strain7." Kawarabayasi Y., Hino Y., Horikawa H., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Otsuka R., Nakazawa H., Takamiya M., Kato Y., Yoshizawa T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Masuda S., Yanagii M., Nishimura M., Yamagishi A., Oshima T., Kikuchi H. DNA Res. 8:123-140(2001) [PubMed: 11572479] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 16993 / JCM 10545 / NBRC 100140 / 7.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000023 Genomic DNA. Translation: BAB67262.1.
RefSeq	NP_378153.1. NC_003106.2.
3D structure databases
ProteinModelPortal	Q96YL5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1460230.
GenomeReviews	Gene locus ST2159 in contig BA000023_GR.
KEGG	sto:ST2159.
NMPDR	fig|273063.1.peg.2321.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG351567.
OMA	YDISARD.
PhylomeDB	Q96YL5.
ProtClustDB	PRK13980.
Enzyme and pathway databases
BioCyc	STOK273063:ST2159-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_SULTO

Accession

Primary (citable) accession number: Q96YL5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	December 1, 2001
Last modified:	December 14, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program