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Protein

Phosphoribosylglycinamide formyltransferase 2

Gene

purT

Organism
Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes two reactions: the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP.UniRule annotation

Catalytic activityi

Formate + ATP + 5'-phospho-ribosylglycinamide = 5'-phosphoribosyl-N-formylglycinamide + ADP + diphosphate.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (formate route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Phosphoribosylglycinamide formyltransferase 2 (purT)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (formate route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei81 – 8115'-phosphoribosylglycinamideUniRule annotation
Binding sitei113 – 1131ATPUniRule annotation
Binding sitei154 – 1541ATPUniRule annotation
Binding sitei202 – 2021ATPUniRule annotation
Metal bindingi272 – 2721MagnesiumUniRule annotation
Metal bindingi284 – 2841MagnesiumUniRule annotation
Binding sitei291 – 29115'-phosphoribosylglycinamideUniRule annotation
Binding sitei361 – 36115'-phosphoribosylglycinamideUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi194 – 1974ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSTOK273063:GJC7-2526-MONOMER.
UniPathwayiUPA00074; UER00127.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylglycinamide formyltransferase 2UniRule annotation (EC:2.1.2.-UniRule annotation)
Short name:
GART 2UniRule annotation
Alternative name(s):
5'-phosphoribosylglycinamide transformylase 2UniRule annotation
Formate-dependent GAR transformylaseUniRule annotation
GAR transformylase 2UniRule annotation
Gene namesi
Name:purTUniRule annotation
Ordered Locus Names:STK_23070
OrganismiSulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Taxonomic identifieri273063 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001015 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 397397Phosphoribosylglycinamide formyltransferase 2PRO_0000319289Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi273063.ST2307.

Structurei

3D structure databases

ProteinModelPortaliQ96Y60.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini118 – 313196ATP-graspUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni21 – 2225'-phosphoribosylglycinamide bindingUniRule annotation
Regioni368 – 36925'-phosphoribosylglycinamide bindingUniRule annotation

Sequence similaritiesi

Belongs to the PurK/PurT family.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01598. Archaea.
COG0027. LUCA.
HOGENOMiHOG000072820.
KOiK08289.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_01643. PurT.
InterProiIPR011761. ATP-grasp.
IPR003135. ATP-grasp_carboxylate-amine.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR005862. PurT.
[Graphical view]
PfamiPF02222. ATP-grasp. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q96Y60-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIGTPLFEG AKKLLWLGGG ELGKEMVIEA QRMGIETVVV DRYDMAPAMH
60 70 80 90 100
VAHRKYVVNM LDGNAIKSII KKENPDAIIT EIEAINTDAL LELESEGYKV
110 120 130 140 150
IPNAKAVKIC MNRIELRKLA AEKVKVPTTQ YGFAENPEEV KKICKDIGYP
160 170 180 190 200
CIIKPEMSSS GHGHEVVYNE SEVEQKFKEA ITHARGKSEQ VIVEEYVKID
210 220 230 240 250
RELTVLTYRY LDDKGVVTKT ITPIEHQRPS DVYYYVESWH PSTVDKDIIE
260 270 280 290 300
RAQEYATRVV NELGGFGIFG VEIIVSGNRV LFSEVSPRPH DTGLVTMASL
310 320 330 340 350
DINEFQIHVR SALGLPTPEV KIVSPAAAHV ILANNEGWAP KFLNVEKALQ
360 370 380 390
IPGVQIRLFG KPSTYYKRRM GVVLATGNTV EEAKEKARKA ASLVLVT
Length:397
Mass (Da):44,257
Last modified:February 26, 2008 - v2
Checksum:iA19BF87145A1A423
GO

Sequence cautioni

The sequence BAB67417.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000023 Genomic DNA. Translation: BAB67417.1. Different initiation.
RefSeqiWP_052846770.1. NC_003106.2.

Genome annotation databases

EnsemblBacteriaiBAB67417; BAB67417; STK_23070.
GeneIDi1460390.
KEGGisto:STK_23070.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000023 Genomic DNA. Translation: BAB67417.1. Different initiation.
RefSeqiWP_052846770.1. NC_003106.2.

3D structure databases

ProteinModelPortaliQ96Y60.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273063.ST2307.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB67417; BAB67417; STK_23070.
GeneIDi1460390.
KEGGisto:STK_23070.

Phylogenomic databases

eggNOGiarCOG01598. Archaea.
COG0027. LUCA.
HOGENOMiHOG000072820.
KOiK08289.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00127.
BioCyciSTOK273063:GJC7-2526-MONOMER.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_01643. PurT.
InterProiIPR011761. ATP-grasp.
IPR003135. ATP-grasp_carboxylate-amine.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR005862. PurT.
[Graphical view]
PfamiPF02222. ATP-grasp. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 16993 / JCM 10545 / NBRC 100140 / 7.

Entry informationi

Entry nameiPURT_SULTO
AccessioniPrimary (citable) accession number: Q96Y60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: February 26, 2008
Last modified: January 20, 2016
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.