Q96X54 (ABFA_ASPAW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable alpha-N-arabinofuranosidase A
Short name=ABF A
Short name=Arabinosidase A
EC=3.2.1.55

Gene names

Name:

abfA

Organism

Aspergillus awamori (Black koji mold)

Taxonomic identifier

105351 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	628 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Alpha-N-arabinofuranosidase involved in the degradation of arabinoxylan, a major component of plant hemicellulose. Acts only on small linear 1,5-alpha-linked L-arabinofuranosyl oligosaccharides By similarity.
Catalytic activity	Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.
Pathway	Glycan metabolism; L-arabinan degradation.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 51 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	L-arabinose metabolic process Inferred from electronic annotation. Source: InterPro arabinan catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway arabinose metabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	extracellular region Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	alpha-N-arabinofuranosidase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Ref.1
	Chain	26 – 628	603	Probable alpha-N-arabinofuranosidase A		PRO_0000394596
Amino acid modifications
	Glycosylation	36	1	N-linked (GlcNAc...) Potential
	Glycosylation	51	1	N-linked (GlcNAc...) Potential
	Glycosylation	74	1	N-linked (GlcNAc...) Potential
	Glycosylation	152	1	N-linked (GlcNAc...) Potential
	Glycosylation	171	1	N-linked (GlcNAc...) Potential
	Glycosylation	260	1	N-linked (GlcNAc...) Potential
	Glycosylation	359	1	N-linked (GlcNAc...) Potential
	Glycosylation	493	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q96X54 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 84E4AF25C4805BE4	FASTA	628	68,007
	10 20 30 40 50 60 MVAFSALSGV SALSLLLCLV QHAHGVSLKV STQGGNSSSP ILYGFMFEDI NHSGDGGIYG 70 80 90 100 110 120 QLLQNPGLQG TTPNLTAWAA VGDATIAIDG DSPLTSAIPS TIKLDVADDA TGAVGLTNEG 130 140 150 160 170 180 YWGIPVDGSE FQSSFWIKGD YSGDITVRLV GNYTGTEYGS ATITHTSTAD NFTQASVKFP 190 200 210 220 230 240 TTKAPDGNVL YELTVDGSVA AGSSLNFGYL TLFGETYKSR ENGLKPQLAN VLADMKGSFL 250 260 270 280 290 300 RFPGGNNLEG NSAENRWKWN ETIGDLWDRP GREGTWTYYN TDGLGLHEYF YWCEDLGLVP 310 320 330 340 350 360 VLGVWDGFAL ESGGNTPITG DALTPYIDDV LNELEYILGD TSTTYGAWRA ANGQEEPWNL 370 380 390 400 410 420 TMVEIGNEDM LGGGCESYAE RFTAFYDAIH AAYPDLILIA STSEADCLPE SMPEGSWVDY 430 440 450 460 470 480 HDYSTPDGLV GQFNYFDNLY RSVPYFIGEY SRWEIDWPNM KGSVSEAVFM IGFERNSDVV 490 500 510 520 530 540 KMAAYAPLLQ LVNSTQWTPD LIGYTQSPDD IFLSTSYYVQ EMFSRNRGDT IKEVTSDSDF 550 560 570 580 590 600 GPLYWVASSA GDSYYVKLAN YGSETQDLTV SIPGTSTGKL TVLADNDPDA YNSDTQTLVT 610 620 PSESTVQASN GTFTFSLPAW AVAVLAAN

References

[1]

"Role of two alpha-L-arabinofuranosidases in arabinoxylan degradation and characteristics of the encoding genes from shochu koji molds, Aspergillus kawachii and Aspergillus awamori." Koseki T., Okuda M., Sudoh S., Kizaki Y., Iwano K., Aramaki I., Matsuzawa H. J. Biosci. Bioeng. 96:232-241(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 26-43. Strain: ATCC 38854 / NBRC 4033.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB046702 Genomic DNA. Translation: BAB21568.2.
3D structure databases
ProteinModelPortal	Q96X54.
ModBase	Search...
Protein family/group databases
CAZy	GH51. Glycoside Hydrolase Family 51.
mycoCLAP	ABF51A_ASPAW.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00667.
Family and domain databases
InterPro	IPR010720. Alpha-L-AF_C. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF06964. Alpha-L-AF_C. 1 hit. [Graphical view]
SMART	SM00813. Alpha-L-AF_C. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ABFA_ASPAW

Accession

Primary (citable) accession number: Q96X54

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 15, 2010
Last sequence update:	December 1, 2001
Last modified:	February 6, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program