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Protein

Enolase

Gene

enoA

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (AFUA_5G01030), Glyceraldehyde-3-phosphate dehydrogenase (AFUA_5G01970)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Enolase (enoA)
  5. Pyruvate kinase (AFUA_6G07430)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei159SubstrateBy similarity1
Binding sitei168SubstrateBy similarity1
Active sitei211Proton donorBy similarity1
Metal bindingi246MagnesiumBy similarity1
Metal bindingi297MagnesiumBy similarity1
Binding sitei297SubstrateBy similarity1
Metal bindingi322MagnesiumBy similarity1
Binding sitei322SubstrateBy similarity1
Active sitei347Proton acceptorBy similarity1
Binding sitei398SubstrateBy similarity1

GO - Molecular functioni

  • IgE binding Source: AspGD
  • magnesium ion binding Source: InterPro
  • phosphopyruvate hydratase activity Source: UniProtKB-EC

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase
2-phosphoglycerate dehydratase
Allergen: Asp f 22
Gene namesi
Name:enoA
ORF Names:AFUA_6G06770
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000002530 Componentsi: Chromosome 6, Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:Afu6g06770

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Binds to IgE.1 Publication

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3116 Asp f 22.0101
72 Asp f 22

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001340412 – 438EnolaseAdd BLAST437

Proteomic databases

PRIDEiQ96X30

PTM databases

SwissPalmiQ96X30

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi5085.CADAFUBP00007078

Structurei

3D structure databases

ProteinModelPortaliQ96X30
SMRiQ96X30
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni374 – 377Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

HOGENOMiHOG000072174
InParanoidiQ96X30
KOiK01689
OMAiEFMIIPV
OrthoDBiEOG092C2W5X

Family and domain databases

CDDicd03313 enolase, 1 hit
Gene3Di3.20.20.120, 1 hit
3.30.390.10, 1 hit
HAMAPiMF_00318 Enolase, 1 hit
InterProiView protein in InterPro
IPR000941 Enolase
IPR036849 Enolase-like_C_sf
IPR029017 Enolase-like_N
IPR034390 Enolase-like_superfamily
IPR020810 Enolase_C
IPR020809 Enolase_CS
IPR020811 Enolase_N
PANTHERiPTHR11902 PTHR11902, 1 hit
PfamiView protein in Pfam
PF00113 Enolase_C, 1 hit
PF03952 Enolase_N, 1 hit
PIRSFiPIRSF001400 Enolase, 1 hit
PRINTSiPR00148 ENOLASE
SFLDiSFLDG00178 enolase, 1 hit
SFLDS00001 Enolase, 1 hit
SMARTiView protein in SMART
SM01192 Enolase_C, 1 hit
SM01193 Enolase_N, 1 hit
SUPFAMiSSF51604 SSF51604, 1 hit
TIGRFAMsiTIGR01060 eno, 1 hit
PROSITEiView protein in PROSITE
PS00164 ENOLASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q96X30-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPISKIHARS VYDSRGNPTV EVDVVTETGL HRAIVPSGAS TGQHEAHELR
60 70 80 90 100
DGDKTQWGGK GVLKAVKNVN ETIGPALIKE NIDVKDQSKV DEFLNKLDGT
110 120 130 140 150
ANKSNLGANA ILGVSLAVAK AGAAEKGVPL YAHISDLAGT KKPYVLPVPF
160 170 180 190 200
QNVLNGGSHA GGRLAFQEFM IVPDSAPSFS EALRQGAEVY QKLKALAKKK
210 220 230 240 250
YGQSAGNVGD EGGVAPDIQT AEEALDLITE AIEQAGYTGK IKIAMDVASS
260 270 280 290 300
EFYKADVKKY DLDFKNPESD PSKWLTYEQL ADLYKSLAAK YPIVSIEDPF
310 320 330 340 350
AEDDWEAWSY FYKTSDFQIV GDDLTVTNPG RIKKAIELKS CNALLLKVNQ
360 370 380 390 400
IGTLTESIQA AKDSYADNWG VMVSHRSGET EDVTIADIAV GLRSGQIKTG
410 420 430
APCRSERLAK LNQILRIEEE LGENAVYAGS KFRTAVNL
Length:438
Mass (Da):47,305
Last modified:January 23, 2007 - v3
Checksum:i821212CBDCBAEA2A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25V → A in AAK49451 (PubMed:11979043).Curated1
Sequence conflicti425A → T in AAK49451 (PubMed:11979043).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF284645 mRNA Translation: AAK49451.1
AAHF01000006 Genomic DNA Translation: EAL88532.1
RefSeqiXP_750570.1, XM_745477.1

Genome annotation databases

EnsemblFungiiCADAFUAT00001960; CADAFUAP00001960; CADAFUAG00001960
GeneIDi3508747
KEGGiafm:AFUA_6G06770

Similar proteinsi

Entry informationi

Entry nameiENO_ASPFU
AccessioniPrimary (citable) accession number: Q96X30
Secondary accession number(s): Q4WND2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 113 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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