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Protein

Isocitrate lyase

Gene

icl1

Organism
Penicillium marneffei
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle. Required for growth on ethanol or acetate, but dispensable when fermentable carbon sources are available. Acts also on 2-methylisocitrate.By similarity

Catalytic activityi

Isocitrate = succinate + glyoxylate.By similarity
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate.By similarity

Cofactori

Mg2+By similarity

Pathway: glyoxylate cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from isocitrate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Isocitrate lyase (icl1)
  2. no protein annotated in this organism
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi170 – 1701MagnesiumBy similarity
Active sitei208 – 2081Proton acceptorBy similarity
Binding sitei245 – 2451SubstrateBy similarity
Binding sitei459 – 4591SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate lyaseBy similarity (EC:4.1.3.1By similarity)
Short name:
ICLCurated
Short name:
IsocitraseCurated
Short name:
IsocitrataseCurated
Alternative name(s):
Methylisocitrate lyaseBy similarity (EC:4.1.3.30By similarity)
Short name:
MICACurated
Threo-D(S)-isocitrate glyoxylate-lyaseCurated
Gene namesi
Name:icl1By similarity
OrganismiPenicillium marneffei
Taxonomic identifieri37727 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesTrichocomaceaeTalaromyces

Subcellular locationi

  • Glyoxysome By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 540540Isocitrate lyasePRO_0000068795Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi441960.XP_002147540.1.

Structurei

3D structure databases

ProteinModelPortaliQ96WZ5.
SMRiQ96WZ5. Positions 9-522.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni99 – 1013Substrate bindingBy similarity
Regioni209 – 2102Substrate bindingBy similarity
Regioni425 – 4295Substrate bindingBy similarity

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.20.60. 2 hits.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERiPTHR21631:SF3. PTHR21631:SF3. 1 hit.
PfamiPF00463. ICL. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q96WZ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASTNFDLED QKYLEDVNAV KQWWTDSRWR YTKRPFTAEQ IVAKRGTLKI
60 70 80 90 100
EYPSNTQAKK LWKILEERFN DKTVSYTYGC LEPTMLTQMI KYLDTIYVSG
110 120 130 140 150
WQSSSTASST DEPSPDLADY PMNTVPNKVN QLFMAQLFHD RKQREERITT
160 170 180 190 200
PREKRSSVQN YDYLRPIIAD ADTGHGGLTA VMKLTKLFVE RGAAGIHIED
210 220 230 240 250
QAPGTKKCGH MAGKVLVPIS EHINRLVAIR AQADIMGTDL LAIARTDSEA
260 270 280 290 300
ATLITSTIDY RDHAYLLGST NPSLQPLNDL MVAAEQSGKS GEQLQAIEDS
310 320 330 340 350
WIAQAGIKKF DDAVIDTINQ GSFANKKELI NRYLTAAKGK SNSEARAIAK
360 370 380 390 400
GITGMDIYWN WDAPRTRGGF YRYQGGTQCA VNRAVAYAPF ADLIWMESKL
410 420 430 440 450
PDYKQAKEFA DGVHAVWPEQ KLAYNLSPSF NWKAAMSKEE QLTYIKRLGK
460 470 480 490 500
LGYCWQFITL AGLHSTALIS DQFASAYAKQ GMRAYGELIQ EPEMERKVDI
510 520 530 540
VTHQKWSGAN LIDHSLAMVT GGISSTAAMG KGVTEDQFKS
Length:540
Mass (Da):60,493
Last modified:December 1, 2001 - v1
Checksum:i3544DC375A692E1B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF373018 Genomic DNA. Translation: AAK54240.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF373018 Genomic DNA. Translation: AAK54240.1.

3D structure databases

ProteinModelPortaliQ96WZ5.
SMRiQ96WZ5. Positions 9-522.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi441960.XP_002147540.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.

Family and domain databases

Gene3Di3.20.20.60. 2 hits.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERiPTHR21631:SF3. PTHR21631:SF3. 1 hit.
PfamiPF00463. ICL. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of the isocitrate lyase gene from the pathogenic fungus, Penicillium marneffei."
    Haycocks N.G., McGinnis M.R., Cooper C.R. Jr.
    Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: PM30.

Entry informationi

Entry nameiACEA_PENMA
AccessioniPrimary (citable) accession number: Q96WZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: December 1, 2001
Last modified: June 24, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.