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Protein

Alpha-1,3/1,6-mannosyltransferase alg2

Gene

alg2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Mannosylates Man2GlcNAc(2)-dolichol diphosphate and Man1GlcNAc(2)-dolichol diphosphate to form Man3GlcNAc(2)-dolichol diphosphate.By similarity

Catalytic activityi

GDP-D-mannose + D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = GDP + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-GlcNAc-diphosphodolichol.
GDP-D-mannose + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = GDP + D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-SPO-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,3/1,6-mannosyltransferase alg2 (EC:2.4.1.132, EC:2.4.1.257)
Alternative name(s):
Asparagine-linked glycosylation protein 2
GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
GDP-Man:Man(1)GlcNAc(2)-PP-dolichol mannosyltransferase
GDP-Man:Man(2)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
Gene namesi
Name:alg2
ORF Names:pi010, SPBC11B10.01, SPBC32H8.14
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC11B10.01.
PomBaseiSPBC11B10.01. alg2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei82 – 104HelicalSequence analysisAdd BLAST23
Transmembranei118 – 138HelicalSequence analysisAdd BLAST21
Transmembranei443 – 463HelicalSequence analysisAdd BLAST21
Transmembranei481 – 501HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000802691 – 506Alpha-1,3/1,6-mannosyltransferase alg2Add BLAST506

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi299N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ96WW6.
PRIDEiQ96WW6.

Interactioni

Protein-protein interaction databases

BioGridi276402. 1 interactor.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000177048.
InParanoidiQ96WW6.
KOiK03843.
OMAiKTHCFEE.
OrthoDBiEOG092C1IJD.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q96WW6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQENSVHRS STKKTPIKIA FIHPDLGIGG AERLVVDAAV GLQSLGKEVV
60 70 80 90 100
VFTSHCDKKH CFEEIRDGTI KVKVYGDWLP SSIFGRLSIF CSSLRQVYLT
110 120 130 140 150
MILLTNYMHF DAIIVDQLST CVPFLLLASQ MILFYCHFPD KYLAKRGGIL
160 170 180 190 200
KKLYRIPFDT VEAESVRLAD RIVVNSKFTA SVFKKAFPKI RKPLRIVHPC
210 220 230 240 250
VDIEAASKPL EFQLPEKILQ RKLLISVNRF ERKKDIRLAI DAFSALRDLS
260 270 280 290 300
ANRFPEYLLL VAGGYDIRVS ENRRYLKELQ EFCEQKDLSY TTVKDNWDNI
310 320 330 340 350
TVAPSTNVLF LLSVPSKVRD ALISSSRILL YTPENEHFGI VPLEAMLRKV
360 370 380 390 400
PVLAQTNGGP LETVIDGKNG WLRPRDAKIW GNVIYEATTS TTYDTAAMGE
410 420 430 440 450
AGSEWVKNEF STDAMARKFE SEIMSGIRSI TPEKRLMRRV NGLLAVFVLF
460 470 480 490 500
MLFWGTCIIA ATVPFAIIKL YFAQTYSSVK LGFMLGTCIV SVSFLTFTVY

AKLTNL
Length:506
Mass (Da):57,167
Last modified:July 11, 2012 - v2
Checksum:iCEA3EFC371A97D0D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB004534 Genomic DNA. Translation: BAA21387.2.
CU329671 Genomic DNA. Translation: CAC37504.3.
RefSeqiNP_595621.3. NM_001021515.2.

Genome annotation databases

EnsemblFungiiSPBC11B10.01.1; SPBC11B10.01.1:pep; SPBC11B10.01.
GeneIDi2539854.
KEGGispo:SPBC11B10.01.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB004534 Genomic DNA. Translation: BAA21387.2.
CU329671 Genomic DNA. Translation: CAC37504.3.
RefSeqiNP_595621.3. NM_001021515.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276402. 1 interactor.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Proteomic databases

MaxQBiQ96WW6.
PRIDEiQ96WW6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC11B10.01.1; SPBC11B10.01.1:pep; SPBC11B10.01.
GeneIDi2539854.
KEGGispo:SPBC11B10.01.

Organism-specific databases

EuPathDBiFungiDB:SPBC11B10.01.
PomBaseiSPBC11B10.01. alg2.

Phylogenomic databases

HOGENOMiHOG000177048.
InParanoidiQ96WW6.
KOiK03843.
OMAiKTHCFEE.
OrthoDBiEOG092C1IJD.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-SPO-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

PROiQ96WW6.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALG2_SCHPO
AccessioniPrimary (citable) accession number: Q96WW6
Secondary accession number(s): O13604, Q96WV8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: July 11, 2012
Last modified: November 30, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.