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Q96WV9

- CDK9_SCHPO

UniProt

Q96WV9 - CDK9_SCHPO

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Protein

Probable cyclin-dependent kinase 9

Gene
cdk9, pi014, SPBC32H8.10
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the positive transcription elongation factor b (P-TEFb) which consists of cdk9 and pch1, and which phosphorylates the C-terminal domain (CTD) of RNA polymerase II and spt5.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Enzyme regulationi

May be activated by autophosphorylation or phosphorylation by a separate activating kinase.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei65 – 651ATP By similarity
Active sitei166 – 1661Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi42 – 509ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
  3. protein binding Source: IntAct
  4. protein serine/threonine kinase activity Source: UniProtKB
  5. RNA polymerase II carboxy-terminal domain kinase activity Source: PomBase

GO - Biological processi

  1. phosphorylation of RNA polymerase II C-terminal domain Source: PomBase
  2. protein phosphorylation Source: UniProtKB
  3. regulation of transcription elongation from RNA polymerase II promoter Source: PomBase
  4. transcription from RNA polymerase I promoter Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cyclin-dependent kinase 9 (EC:2.7.11.22, EC:2.7.11.23)
Alternative name(s):
Cell division protein kinase 9
Gene namesi
Name:cdk9
ORF Names:pi014, SPBC32H8.10
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome II

Organism-specific databases

PomBaseiSPBC32H8.10.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: PomBase
  2. positive transcription elongation factor complex b Source: PomBase
  3. P-TEFb-cap methyltransferase complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi65 – 651K → A: Abolishes activity. 1 Publication
Mutagenesisi83 – 831E → A: Defective kinase activity. 1 Publication
Mutagenesisi184 – 1841D → N: Abolishes activity. 1 Publication
Mutagenesisi212 – 2121T → A: Abolishes activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 591591Probable cyclin-dependent kinase 9PRO_0000085808Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei211 – 2111Phosphotyrosine1 Publication
Modified residuei212 – 2121Phosphothreonine1 Publication
Modified residuei565 – 5651Phosphothreonine1 Publication
Modified residuei577 – 5771Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ96WV9.
PRIDEiQ96WV9.

Interactioni

Subunit structurei

Interacts with pch1 cyclin via its N-terminal domain. Via its C-terminal domain, interacts with RNA triphosphatase pct1 which is involved in mRNA capping. Interacts also with pcm1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
pch1O746272EBI-443557,EBI-443575
pct1Q9P6Q62EBI-443557,EBI-443547

Protein-protein interaction databases

BioGridi276771. 18 interactions.
IntActiQ96WV9. 2 interactions.
MINTiMINT-253408.
STRINGi4896.SPBC32H8.10-1.

Structurei

3D structure databases

ProteinModelPortaliQ96WV9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 339304Protein kinaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 385385Interacts with pch1Add
BLAST
Regioni442 – 52382Binds to pct1Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
KOiK15562.
OMAiCHSNERM.
OrthoDBiEOG7K3TWD.
PhylomeDBiQ96WV9.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q96WV9-1 [UniParc]FASTAAdd to Basket

« Hide

MKRSSSVSVE DEKSARRKLD VVPKLHFVGC SHLTDYHLME KLGEGTFGEV    50
YKSQRRKDGK VYALKRILMH TEKEGFPITA IREIKILKSI KHENIIPLSD 100
MTVVRADKKH RRRGSIYMVT PYMDHDLSGL LENPSVKFTE PQIKCYMKQL 150
FAGTKYLHDQ LILHRDLKAA NLLIDNHGIL KIADFGLARV ITEESYANKN 200
PGLPPPNRRE YTGCVVTRWY RSPELLLGER RYTTAIDMWS VGCIMAEMYK 250
GRPILQGSSD LDQLDKIFRL CGSPTQATMP NWEKLPGCEG VRSFPSHPRT 300
LETAFFTFGK EMTSLCGAIL TLNPDERLSA SMALEHEYFT TPPYPANPSE 350
LQSYSASHEY DKRRKREQRD ANSHAFEQTA NGKRQFRFMT RGPSDPWYGI 400
RRPNYNSQPQ YQRGSYNREG GNMDRSRNVN YQPKRQQNFK PLTSDLPQKN 450
SEFSETNAMN QTSNHSHADG QRYYRPEQDR SQRLRNPSDY GRQGRQSSQS 500
QQPAWNVSSR YQNNSKVQTT SRASENADTN KTQHNIKYID SYVPEYSIAR 550
QSANQKTNEQ HPSSTSLHQQ STSDLKSPSF HENSNVDDTP K 591
Length:591
Mass (Da):68,029
Last modified:December 1, 2001 - v1
Checksum:i28DFF6032388E16A
GO

Sequence cautioni

The sequence BAA21391.2 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB004534 Genomic DNA. Translation: BAA21391.2. Different initiation.
CU329671 Genomic DNA. Translation: CAC37500.1.
RefSeqiNP_595616.1. NM_001021511.2.

Genome annotation databases

EnsemblFungiiSPBC32H8.10.1; SPBC32H8.10.1:pep; SPBC32H8.10.
GeneIDi2540239.
KEGGispo:SPBC32H8.10.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB004534 Genomic DNA. Translation: BAA21391.2 . Different initiation.
CU329671 Genomic DNA. Translation: CAC37500.1 .
RefSeqi NP_595616.1. NM_001021511.2.

3D structure databases

ProteinModelPortali Q96WV9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 276771. 18 interactions.
IntActi Q96WV9. 2 interactions.
MINTi MINT-253408.
STRINGi 4896.SPBC32H8.10-1.

Proteomic databases

MaxQBi Q96WV9.
PRIDEi Q96WV9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPBC32H8.10.1 ; SPBC32H8.10.1:pep ; SPBC32H8.10 .
GeneIDi 2540239.
KEGGi spo:SPBC32H8.10.

Organism-specific databases

PomBasei SPBC32H8.10.

Phylogenomic databases

eggNOGi COG0515.
KOi K15562.
OMAi CHSNERM.
OrthoDBi EOG7K3TWD.
PhylomeDBi Q96WV9.

Miscellaneous databases

NextBioi 20801370.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A 38 kb segment containing the cdc2 gene from the left arm of fission yeast chromosome II: sequence analysis and characterization of the genomic DNA and cDNAs encoded on the segment."
    Machida M., Yamazaki S., Kunihiro S., Tanaka T., Kushida N., Jinno K., Haikawa Y., Yamazaki J., Yamamoto S., Sekine M., Oguchi A., Nagai Y., Sakai M., Aoki K., Ogura K., Kudoh Y., Kikuchi H., Zhang M.Q., Yanagida M.
    Yeast 16:71-80(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Interactions between fission yeast Cdk9, its cyclin partner Pch1, and mRNA capping enzyme Pct1 suggest an elongation checkpoint for mRNA quality control."
    Pei Y., Schwer B., Shuman S.
    J. Biol. Chem. 278:7180-7188(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PCH1 AND PCT1, MUTAGENESIS OF LYS-65; GLU-83; ASP-184 AND THR-212.
  4. "Cyclin-dependent kinase 9 (Cdk9) of fission yeast is activated by the CDK-activating kinase Csk1, overlaps functionally with the TFIIH-associated kinase Mcs6, and associates with the mRNA cap methyltransferase Pcm1 in vivo."
    Pei Y., Du H., Singer J., Saint Amour C., Granitto S., Shuman S., Fisher R.P.
    Mol. Cell. Biol. 26:777-788(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PCM1.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-211; THR-212; THR-565 AND SER-577, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiCDK9_SCHPO
AccessioniPrimary (citable) accession number: Q96WV9
Secondary accession number(s): O13607
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2003
Last sequence update: December 1, 2001
Last modified: June 11, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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