ID   COPA_SCHPO              Reviewed;        1207 AA.
AC   Q96WV5;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   27-MAR-2024, entry version 161.
DE   RecName: Full=Putative coatomer subunit alpha;
DE   AltName: Full=Alpha-coat protein;
DE            Short=Alpha-COP;
GN   ORFNames=SPBPJ4664.04;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-409 AND SER-942, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=18257517; DOI=10.1021/pr7006335;
RA   Wilson-Grady J.T., Villen J., Gygi S.P.;
RT   "Phosphoproteome analysis of fission yeast.";
RL   J. Proteome Res. 7:1088-1097(2008).
CC   -!- FUNCTION: The coatomer is a cytosolic protein complex that binds to
CC       dilysine motifs and reversibly associates with Golgi non-clathrin-
CC       coated vesicles, which further mediate biosynthetic protein transport
CC       from the ER, via the Golgi up to the trans Golgi network. Coatomer
CC       complex is required for budding from Golgi membranes, and is essential
CC       for the retrograde Golgi-to-ER transport of dilysine-tagged proteins
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Oligomeric complex that consists of at least the alpha, beta,
CC       beta', gamma, delta, epsilon and zeta subunits. {ECO:0000250}.
CC   -!- INTERACTION:
CC       Q96WV5; P68978; Xeno; NbExp=2; IntAct=EBI-8503699, EBI-4407041;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Golgi apparatus membrane; Peripheral
CC       membrane protein; Cytoplasmic side.
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DR   EMBL; CU329671; CAC38349.1; -; Genomic_DNA.
DR   RefSeq; NP_595279.1; NM_001021186.2.
DR   PDB; 4J87; X-ray; 1.67 A; A=1-327.
DR   PDB; 4J8B; X-ray; 1.88 A; A=1-327.
DR   PDB; 4J8G; X-ray; 1.90 A; A/B=1-327.
DR   PDB; 7S16; X-ray; 1.24 A; A=1-327.
DR   PDB; 7S22; X-ray; 1.75 A; A/B/C=1-327.
DR   PDB; 7S23; X-ray; 1.49 A; A/B/C=1-327.
DR   PDB; 8EVL; X-ray; 1.90 A; A/B/C=1-327.
DR   PDBsum; 4J87; -.
DR   PDBsum; 4J8B; -.
DR   PDBsum; 4J8G; -.
DR   PDBsum; 7S16; -.
DR   PDBsum; 7S22; -.
DR   PDBsum; 7S23; -.
DR   PDBsum; 8EVL; -.
DR   AlphaFoldDB; Q96WV5; -.
DR   SMR; Q96WV5; -.
DR   BioGRID; 277920; 13.
DR   IntAct; Q96WV5; 7.
DR   MINT; Q96WV5; -.
DR   STRING; 284812.Q96WV5; -.
DR   iPTMnet; Q96WV5; -.
DR   MaxQB; Q96WV5; -.
DR   PaxDb; 4896-SPBPJ4664-04-1; -.
DR   EnsemblFungi; SPBPJ4664.04.1; SPBPJ4664.04.1:pep; SPBPJ4664.04.
DR   GeneID; 2541412; -.
DR   KEGG; spo:SPBPJ4664.04; -.
DR   PomBase; SPBPJ4664.04; -.
DR   VEuPathDB; FungiDB:SPBPJ4664.04; -.
DR   eggNOG; KOG0292; Eukaryota.
DR   HOGENOM; CLU_007565_1_0_1; -.
DR   InParanoid; Q96WV5; -.
DR   OMA; EMTYQKQ; -.
DR   PhylomeDB; Q96WV5; -.
DR   Reactome; R-SPO-6807878; COPI-mediated anterograde transport.
DR   Reactome; R-SPO-6811434; COPI-dependent Golgi-to-ER retrograde traffic.
DR   PRO; PR:Q96WV5; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0030126; C:COPI vesicle coat; IBA:GO_Central.
DR   GO; GO:0005737; C:cytoplasm; HDA:PomBase.
DR   GO; GO:0005794; C:Golgi apparatus; HDA:PomBase.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IBA:GO_Central.
DR   GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; IBA:GO_Central.
DR   GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR   GO; GO:0006890; P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum; IBA:GO_Central.
DR   CDD; cd22948; Coatomer_WDAD_alpha; 1.
DR   CDD; cd00200; WD40; 1.
DR   Gene3D; 1.25.40.470; -; 1.
DR   Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR   InterPro; IPR047312; Coatomer_alpha_WD-assoc_reg.
DR   InterPro; IPR016391; Coatomer_asu.
DR   InterPro; IPR010714; Coatomer_asu_C.
DR   InterPro; IPR006692; Coatomer_WD-assoc_reg.
DR   InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   InterPro; IPR001680; WD40_rpt.
DR   PANTHER; PTHR19876; COATOMER; 1.
DR   PANTHER; PTHR19876:SF1; COATOMER SUBUNIT ALPHA; 1.
DR   Pfam; PF04053; Coatomer_WDAD; 1.
DR   Pfam; PF06957; COPI_C; 1.
DR   Pfam; PF00400; WD40; 6.
DR   PIRSF; PIRSF003354; Coatomer_alpha_subunit; 1.
DR   PRINTS; PR00320; GPROTEINBRPT.
DR   SMART; SM00320; WD40; 7.
DR   SUPFAM; SSF82171; DPP6 N-terminal domain-like; 1.
DR   SUPFAM; SSF50978; WD40 repeat-like; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 2.
DR   PROSITE; PS50082; WD_REPEATS_2; 6.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; ER-Golgi transport; Golgi apparatus; Membrane;
KW   Phosphoprotein; Protein transport; Reference proteome; Repeat; Transport;
KW   WD repeat.
FT   CHAIN           1..1207
FT                   /note="Putative coatomer subunit alpha"
FT                   /id="PRO_0000316543"
FT   REPEAT          9..50
FT                   /note="WD 1"
FT   REPEAT          51..90
FT                   /note="WD 2"
FT   REPEAT          93..134
FT                   /note="WD 3"
FT   REPEAT          135..174
FT                   /note="WD 4"
FT   REPEAT          210..249
FT                   /note="WD 5"
FT   REPEAT          254..293
FT                   /note="WD 6"
FT   REPEAT          296..336
FT                   /note="WD 7"
FT   REPEAT          370..411
FT                   /note="WD 8"
FT   MOD_RES         409
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:18257517"
FT   MOD_RES         942
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:18257517"
FT   STRAND          2..10
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   STRAND          14..19
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   STRAND          21..30
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   STRAND          35..39
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   TURN            40..43
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   STRAND          44..49
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   STRAND          56..61
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   STRAND          63..72
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   STRAND          77..81
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   TURN            82..85
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   STRAND          86..91
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   STRAND          98..103
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   STRAND          105..114
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   STRAND          119..123
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   TURN            124..127
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   STRAND          128..133
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   STRAND          140..145
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   STRAND          147..156
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   STRAND          161..165
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   HELIX           167..171
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   TURN            172..174
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   HELIX           181..183
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   STRAND          202..208
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   STRAND          215..220
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   STRAND          222..231
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   STRAND          236..241
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   STRAND          246..252
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   STRAND          259..264
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   STRAND          266..275
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   STRAND          278..284
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   TURN            285..287
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   STRAND          290..295
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   STRAND          301..306
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   STRAND          308..311
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   STRAND          313..317
FT                   /evidence="ECO:0007829|PDB:7S16"
FT   STRAND          320..327
FT                   /evidence="ECO:0007829|PDB:7S16"
SQ   SEQUENCE   1207 AA;  136369 MW;  4AB701FD7310BE29 CRC64;
     MEMLTKFESR SSRAKGVAFH PTQPWILTSL HNGRIQLWDY RMGTLLDRFD GHDGPVRGIA
     FHPTQPLFVS GGDDYKVNVW NYKSRKLLFS LCGHMDYVRV CTFHHEYPWI LSCSDDQTIR
     IWNWQSRNCI AILTGHSHYV MCAAFHPSED LIVSASLDQT VRVWDISGLR MKNAAPVSMS
     LEDQLAQAHN SISNDLFGST DAIVKFVLEG HDRGVNWCAF HPTLPLILSA GDDRLVKLWR
     MTASKAWEVD TCRGHFNNVS CCLFHPHQEL ILSASEDKTI RVWDLNRRTA VQTFRRDNDR
     FWFITVHPKL NLFAAAHDSG VMVFKLERER PAHALNINTL LYVNKEKSIV SYDLLRAQST
     TVASVKHLGS AWLPPRSLSY NPAEKVALLT SSADNGVYEL VNVSSRSNSL PLKDNIKGPG
     DDAIFVARNR FAVFSRSDQT IEIKDLSNKV TKTIQLPEKT RDIFFAGMGH VLLSTATQVH
     LFDLQQKKIV SSFNANRVKY VVWSNDNSQA ALLGKHYVYI VKKNLELITS IHETIRIKSA
     VWVENNVLLY ATLDHLKYAL MSGDTGVIKT LESTLYLVKA KGNMVFALNR AAEPVSFEID
     PTEYLFKLAL LRKDYEQVLH LIQNSNLVGQ AIIAYLQKKG YPEIALQFVE DPSTRFELAL
     ECGNLETALE LARTIDRPEV WSRLASDAMS YGNHKIAEIT FQKLRYFEKL SFLYLITGNA
     EKLQKMAIIA EKRNDTLSLF QNSLYLNEVE SRINILEQAG MYPIAYLTAK SNGLEEKAQQ
     ILSHCNKTEE EIKLPSLGSA FTTPVPVNET YTHNWPLLDT SHSTFEKSLQ ERMEQLAIER
     QEEQESEEEY EEVEQSLMDV VDEMSDLAES VPEEEVDGWE VEDLAPEEAV NDVVDDASAF
     VGADEIFLWK RNSPLAADHI AAGDFESAMK ILNKQVGAIN FSPLKTRFLE IYTASRVYLP
     TISGLDPLVS YVRRNAETAE RSQALPFITR NLASIKSHEL HEAYRLVKAN KILEAQICFR
     SIIYLALTTV ANSEEEADEI SALIDECCRY IVALSCELER RRLGEEDTKR ALELSYYFAS
     ADLQPMHSII ALRLAINASH KLKNYKSASF LGNKLLQLAE SGPAAEAANR AITLGDRNPH
     DAFEIEYDPH VEMRICPKTL TPVYSGDDFD VCSVCGAVYH KGYVNEVCTV CDVGGIGQKG
     TGRRFFA
//