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Protein

Putative coatomer subunit alpha

Gene

SPBPJ4664.04

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-SPO-6807878. COPI-mediated anterograde transport.
R-SPO-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative coatomer subunit alpha
Alternative name(s):
Alpha-coat protein
Short name:
Alpha-COP
Gene namesi
ORF Names:SPBPJ4664.04
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBPJ4664.04.
PomBaseiSPBPJ4664.04.

Subcellular locationi

GO - Cellular componenti

  • COPI-coated vesicle Source: PomBase
  • COPI vesicle coat Source: PomBase
  • cytoplasm Source: PomBase
  • Golgi apparatus Source: PomBase
  • Golgi membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003165431 – 1207Putative coatomer subunit alphaAdd BLAST1207

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei409Phosphoserine1 Publication1
Modified residuei942Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ96WV5.
PRIDEiQ96WV5.

PTM databases

iPTMnetiQ96WV5.

Interactioni

Subunit structurei

Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
P689782EBI-8503699,EBI-4407041From a different organism.
AWRI796_1468E7KC072EBI-8503699,EBI-8503574From a different organism.

Protein-protein interaction databases

BioGridi277920. 7 interactors.
IntActiQ96WV5. 8 interactors.
MINTiMINT-4701710.

Structurei

Secondary structure

11207
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 10Combined sources8
Beta strandi14 – 19Combined sources6
Beta strandi21 – 30Combined sources10
Beta strandi33 – 39Combined sources7
Turni40 – 43Combined sources4
Beta strandi44 – 50Combined sources7
Beta strandi56 – 61Combined sources6
Beta strandi63 – 72Combined sources10
Beta strandi77 – 81Combined sources5
Turni82 – 85Combined sources4
Beta strandi86 – 91Combined sources6
Beta strandi98 – 103Combined sources6
Beta strandi105 – 114Combined sources10
Beta strandi119 – 123Combined sources5
Turni124 – 127Combined sources4
Beta strandi128 – 133Combined sources6
Beta strandi140 – 145Combined sources6
Beta strandi147 – 156Combined sources10
Beta strandi159 – 165Combined sources7
Helixi167 – 174Combined sources8
Helixi181 – 183Combined sources3
Beta strandi202 – 209Combined sources8
Beta strandi215 – 220Combined sources6
Beta strandi222 – 231Combined sources10
Beta strandi234 – 241Combined sources8
Beta strandi246 – 253Combined sources8
Beta strandi259 – 264Combined sources6
Beta strandi266 – 275Combined sources10
Beta strandi278 – 284Combined sources7
Turni285 – 287Combined sources3
Beta strandi290 – 295Combined sources6
Beta strandi301 – 306Combined sources6
Beta strandi308 – 311Combined sources4
Beta strandi313 – 317Combined sources5
Beta strandi320 – 326Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4J87X-ray1.67A1-327[»]
4J8BX-ray1.88A1-327[»]
4J8GX-ray1.90A/B1-327[»]
ProteinModelPortaliQ96WV5.
SMRiQ96WV5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati9 – 50WD 1Add BLAST42
Repeati51 – 90WD 2Add BLAST40
Repeati93 – 134WD 3Add BLAST42
Repeati135 – 174WD 4Add BLAST40
Repeati210 – 249WD 5Add BLAST40
Repeati254 – 293WD 6Add BLAST40
Repeati296 – 336WD 7Add BLAST41
Repeati370 – 411WD 8Add BLAST42

Sequence similaritiesi

Contains 8 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

HOGENOMiHOG000195913.
InParanoidiQ96WV5.
KOiK05236.
OMAiQFKGNLC.
OrthoDBiEOG092C10HM.
PhylomeDBiQ96WV5.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR016391. Coatomer_asu.
IPR010714. Coatomer_asu_C.
IPR006692. Coatomer_WD-assoc_reg.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF04053. Coatomer_WDAD. 1 hit.
PF06957. COPI_C. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
PIRSFiPIRSF003354. Coatomer_alpha_subunit. 1 hit.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q96WV5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEMLTKFESR SSRAKGVAFH PTQPWILTSL HNGRIQLWDY RMGTLLDRFD
60 70 80 90 100
GHDGPVRGIA FHPTQPLFVS GGDDYKVNVW NYKSRKLLFS LCGHMDYVRV
110 120 130 140 150
CTFHHEYPWI LSCSDDQTIR IWNWQSRNCI AILTGHSHYV MCAAFHPSED
160 170 180 190 200
LIVSASLDQT VRVWDISGLR MKNAAPVSMS LEDQLAQAHN SISNDLFGST
210 220 230 240 250
DAIVKFVLEG HDRGVNWCAF HPTLPLILSA GDDRLVKLWR MTASKAWEVD
260 270 280 290 300
TCRGHFNNVS CCLFHPHQEL ILSASEDKTI RVWDLNRRTA VQTFRRDNDR
310 320 330 340 350
FWFITVHPKL NLFAAAHDSG VMVFKLERER PAHALNINTL LYVNKEKSIV
360 370 380 390 400
SYDLLRAQST TVASVKHLGS AWLPPRSLSY NPAEKVALLT SSADNGVYEL
410 420 430 440 450
VNVSSRSNSL PLKDNIKGPG DDAIFVARNR FAVFSRSDQT IEIKDLSNKV
460 470 480 490 500
TKTIQLPEKT RDIFFAGMGH VLLSTATQVH LFDLQQKKIV SSFNANRVKY
510 520 530 540 550
VVWSNDNSQA ALLGKHYVYI VKKNLELITS IHETIRIKSA VWVENNVLLY
560 570 580 590 600
ATLDHLKYAL MSGDTGVIKT LESTLYLVKA KGNMVFALNR AAEPVSFEID
610 620 630 640 650
PTEYLFKLAL LRKDYEQVLH LIQNSNLVGQ AIIAYLQKKG YPEIALQFVE
660 670 680 690 700
DPSTRFELAL ECGNLETALE LARTIDRPEV WSRLASDAMS YGNHKIAEIT
710 720 730 740 750
FQKLRYFEKL SFLYLITGNA EKLQKMAIIA EKRNDTLSLF QNSLYLNEVE
760 770 780 790 800
SRINILEQAG MYPIAYLTAK SNGLEEKAQQ ILSHCNKTEE EIKLPSLGSA
810 820 830 840 850
FTTPVPVNET YTHNWPLLDT SHSTFEKSLQ ERMEQLAIER QEEQESEEEY
860 870 880 890 900
EEVEQSLMDV VDEMSDLAES VPEEEVDGWE VEDLAPEEAV NDVVDDASAF
910 920 930 940 950
VGADEIFLWK RNSPLAADHI AAGDFESAMK ILNKQVGAIN FSPLKTRFLE
960 970 980 990 1000
IYTASRVYLP TISGLDPLVS YVRRNAETAE RSQALPFITR NLASIKSHEL
1010 1020 1030 1040 1050
HEAYRLVKAN KILEAQICFR SIIYLALTTV ANSEEEADEI SALIDECCRY
1060 1070 1080 1090 1100
IVALSCELER RRLGEEDTKR ALELSYYFAS ADLQPMHSII ALRLAINASH
1110 1120 1130 1140 1150
KLKNYKSASF LGNKLLQLAE SGPAAEAANR AITLGDRNPH DAFEIEYDPH
1160 1170 1180 1190 1200
VEMRICPKTL TPVYSGDDFD VCSVCGAVYH KGYVNEVCTV CDVGGIGQKG

TGRRFFA
Length:1,207
Mass (Da):136,369
Last modified:December 1, 2001 - v1
Checksum:i4AB701FD7310BE29
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAC38349.1.
RefSeqiNP_595279.1. NM_001021186.2.

Genome annotation databases

EnsemblFungiiSPBPJ4664.04.1; SPBPJ4664.04.1:pep; SPBPJ4664.04.
GeneIDi2541412.
KEGGispo:SPBPJ4664.04.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAC38349.1.
RefSeqiNP_595279.1. NM_001021186.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4J87X-ray1.67A1-327[»]
4J8BX-ray1.88A1-327[»]
4J8GX-ray1.90A/B1-327[»]
ProteinModelPortaliQ96WV5.
SMRiQ96WV5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277920. 7 interactors.
IntActiQ96WV5. 8 interactors.
MINTiMINT-4701710.

PTM databases

iPTMnetiQ96WV5.

Proteomic databases

MaxQBiQ96WV5.
PRIDEiQ96WV5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBPJ4664.04.1; SPBPJ4664.04.1:pep; SPBPJ4664.04.
GeneIDi2541412.
KEGGispo:SPBPJ4664.04.

Organism-specific databases

EuPathDBiFungiDB:SPBPJ4664.04.
PomBaseiSPBPJ4664.04.

Phylogenomic databases

HOGENOMiHOG000195913.
InParanoidiQ96WV5.
KOiK05236.
OMAiQFKGNLC.
OrthoDBiEOG092C10HM.
PhylomeDBiQ96WV5.

Enzyme and pathway databases

ReactomeiR-SPO-6807878. COPI-mediated anterograde transport.
R-SPO-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Miscellaneous databases

PROiQ96WV5.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR016391. Coatomer_asu.
IPR010714. Coatomer_asu_C.
IPR006692. Coatomer_WD-assoc_reg.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF04053. Coatomer_WDAD. 1 hit.
PF06957. COPI_C. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
PIRSFiPIRSF003354. Coatomer_alpha_subunit. 1 hit.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCOPA_SCHPO
AccessioniPrimary (citable) accession number: Q96WV5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.