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Q96WV1

- TRMB_SCHPO

UniProt

Q96WV1 - TRMB_SCHPO

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trm8

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei88 – 881S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei170 – 1701S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Active sitei173 – 1731UniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: PomBase
  2. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA (guanine-N7)-methylation Source: GOC
  2. tRNA methylation Source: PomBase
  3. tRNA modification Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
Transfer RNA methyltransferase 8UniRule annotation
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trm8
ORF Names:SPCPB16A4.04c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome III

Organism-specific databases

PomBaseiSPCPB16A4.04c.

Subcellular locationi

Nucleus UniRule annotation

GO - Cellular componenti

  1. nucleolus Source: PomBase
  2. nucleus Source: PomBase
  3. protein complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 273273tRNA (guanine-N(7)-)-methyltransferasePRO_0000171436Add
BLAST

Proteomic databases

MaxQBiQ96WV1.

Interactioni

Subunit structurei

Forms a complex with trm82.UniRule annotation

Protein-protein interaction databases

BioGridi276134. 16 interactions.
MINTiMINT-4701684.
STRINGi4896.SPCPB16A4.04c-1.

Structurei

3D structure databases

ProteinModelPortaliQ96WV1.
SMRiQ96WV1. Positions 56-273.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni111 – 1122S-adenosyl-L-methionine bindingUniRule annotation
Regioni150 – 1512S-adenosyl-L-methionine bindingUniRule annotation
Regioni248 – 2503S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000260965.
InParanoidiQ96WV1.
KOiK03439.
OMAiRAHSNPI.
OrthoDBiEOG708W9T.
PhylomeDBiQ96WV1.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q96WV1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSATAKKSAA QLQREEEEAR KKLKRLSKQG GVEGRLPMKR LFRQRAHANV
60 70 80 90 100
LSDHELEYPR SPSEMDWSPY YPDFDVESNK KVEIVDIGCG YGGLTVALGP
110 120 130 140 150
QFPDTLVLGM EIRMQVSDYL KEKIQALRYR ADHEEPVPGG YKNISVLRMN
160 170 180 190 200
CQKFLPNFFE KGQLSKMFFC FPDPHFKARK HKNRIITSTL ASEYAYFIRP
210 220 230 240 250
HGTLYTITDV EELHVWMAQH LDAHPLFRRF TKEEEENDIC VTLMTNETEE
260 270
GKKVARNGGK KFVACYERIP NPK
Length:273
Mass (Da):31,622
Last modified:December 1, 2001 - v1
Checksum:iCD9814860E37855E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329672 Genomic DNA. Translation: CAC39323.1.
RefSeqiNP_588028.1. NM_001023019.2.

Genome annotation databases

EnsemblFungiiSPCPB16A4.04c.1; SPCPB16A4.04c.1:pep; SPCPB16A4.04c.
GeneIDi2539574.
KEGGispo:SPCPB16A4.04c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329672 Genomic DNA. Translation: CAC39323.1 .
RefSeqi NP_588028.1. NM_001023019.2.

3D structure databases

ProteinModelPortali Q96WV1.
SMRi Q96WV1. Positions 56-273.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 276134. 16 interactions.
MINTi MINT-4701684.
STRINGi 4896.SPCPB16A4.04c-1.

Proteomic databases

MaxQBi Q96WV1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPCPB16A4.04c.1 ; SPCPB16A4.04c.1:pep ; SPCPB16A4.04c .
GeneIDi 2539574.
KEGGi spo:SPCPB16A4.04c.

Organism-specific databases

PomBasei SPCPB16A4.04c.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000260965.
InParanoidi Q96WV1.
KOi K03439.
OMAi RAHSNPI.
OrthoDBi EOG708W9T.
PhylomeDBi Q96WV1.

Enzyme and pathway databases

UniPathwayi UPA00989 .

Miscellaneous databases

NextBioi 20800733.
PROi Q96WV1.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiTRMB_SCHPO
AccessioniPrimary (citable) accession number: Q96WV1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: December 1, 2001
Last modified: October 29, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3