Q96WV1 (TRMB_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
Transfer RNA methyltransferase 8
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

Name:	trm8
ORF Names:	SPCPB16A4.04c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA.
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
Subunit structure	Forms a complex with trm82 By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine tRNA-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	nucleolus Inferred from direct assay PubMed 16823372. Source: PomBase protein complex Inferred from sequence orthology. Source: PomBase
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from sequence orthology. Source: PomBase tRNA binding Inferred by curator. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	tRNA (guanine-N(7)-)-methyltransferase		PRO_0000171436
Regions
	Region	111 – 112	2	S-adenosyl-L-methionine binding By similarity
	Region	248 – 250	3	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	173	1	By similarity
	Binding site	88	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	170	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q96WV1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: CD9814860E37855E	FASTA	273	31,622
	10 20 30 40 50 60 MSATAKKSAA QLQREEEEAR KKLKRLSKQG GVEGRLPMKR LFRQRAHANV LSDHELEYPR 70 80 90 100 110 120 SPSEMDWSPY YPDFDVESNK KVEIVDIGCG YGGLTVALGP QFPDTLVLGM EIRMQVSDYL 130 140 150 160 170 180 KEKIQALRYR ADHEEPVPGG YKNISVLRMN CQKFLPNFFE KGQLSKMFFC FPDPHFKARK 190 200 210 220 230 240 HKNRIITSTL ASEYAYFIRP HGTLYTITDV EELHVWMAQH LDAHPLFRRF TKEEEENDIC 250 260 270 VTLMTNETEE GKKVARNGGK KFVACYERIP NPK

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329672 Genomic DNA. Translation: CAC39323.1.
RefSeq	NP_588028.1. NM_001023019.2.
3D structure databases
ProteinModelPortal	Q96WV1.
SMR	Q96WV1. Positions 56-273.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPCPB16A4.04c-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPCPB16A4.04c.1; SPCPB16A4.04c.1:pep; SPCPB16A4.04c.
GeneID	2539574.
KEGG	spo:SPCPB16A4.04c.
Organism-specific databases
PomBase	SPCPB16A4.04c.
Phylogenomic databases
eggNOG	COG0220.
HOGENOM	HOG000260965.
KO	K03439.
OMA	RAHSNPI.
OrthoDB	EOG4M3DJF.
Enzyme and pathway databases
UniPathway	UPA00989.
Family and domain databases
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. IPR025763. tRNA_(Gua-N-7)_MeTrfase_euk. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...
Other
NextBio	20800733.

Entry information

Entry name

TRMB_SCHPO

Accession

Primary (citable) accession number: Q96WV1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2003
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program