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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trm8

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei88S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei170S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei173UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
Transfer RNA methyltransferase 8UniRule annotation
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trm8
ORF Names:SPCPB16A4.04c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCPB16A4.04c
PomBaseiSPCPB16A4.04c trm8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001714361 – 273tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST273

Proteomic databases

MaxQBiQ96WV1
PaxDbiQ96WV1
PRIDEiQ96WV1

Interactioni

Subunit structurei

Forms a complex with trm82.UniRule annotation

Protein-protein interaction databases

BioGridi276134, 16 interactors
STRINGi4896.SPCPB16A4.04c.1

Structurei

3D structure databases

ProteinModelPortaliQ96WV1
SMRiQ96WV1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni111 – 112S-adenosyl-L-methionine bindingUniRule annotation2
Regioni150 – 151S-adenosyl-L-methionine bindingUniRule annotation2
Regioni248 – 250S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000260965
InParanoidiQ96WV1
KOiK03439
OMAiIGMEIRV
OrthoDBiEOG092C3FCG
PhylomeDBiQ96WV1

Family and domain databases

HAMAPiMF_03055 tRNA_methyltr_TrmB_euk, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR025763 Trm8_euk
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
PTHR23417:SF16 PTHR23417:SF16, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

Q96WV1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSATAKKSAA QLQREEEEAR KKLKRLSKQG GVEGRLPMKR LFRQRAHANV
60 70 80 90 100
LSDHELEYPR SPSEMDWSPY YPDFDVESNK KVEIVDIGCG YGGLTVALGP
110 120 130 140 150
QFPDTLVLGM EIRMQVSDYL KEKIQALRYR ADHEEPVPGG YKNISVLRMN
160 170 180 190 200
CQKFLPNFFE KGQLSKMFFC FPDPHFKARK HKNRIITSTL ASEYAYFIRP
210 220 230 240 250
HGTLYTITDV EELHVWMAQH LDAHPLFRRF TKEEEENDIC VTLMTNETEE
260 270
GKKVARNGGK KFVACYERIP NPK
Length:273
Mass (Da):31,622
Last modified:December 1, 2001 - v1
Checksum:iCD9814860E37855E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA Translation: CAC39323.1
RefSeqiNP_588028.1, NM_001023019.2

Genome annotation databases

EnsemblFungiiSPCPB16A4.04c.1; SPCPB16A4.04c.1:pep; SPCPB16A4.04c
GeneIDi2539574
KEGGispo:SPCPB16A4.04c

Similar proteinsi

Entry informationi

Entry nameiTRMB_SCHPO
AccessioniPrimary (citable) accession number: Q96WV1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: December 1, 2001
Last modified: May 23, 2018
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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