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Q96WQ9

- EGLD_ASPKW

UniProt

Q96WQ9 - EGLD_ASPKW

Protein

Probable endo-beta-1,4-glucanase D

Gene

eglD

Organism
Aspergillus kawachii (strain NBRC 4308) (White koji mold) (Aspergillus awamori var. kawachi)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Has endoglucanase activity on substrates containing beta-1,4 glycosidic bonds, like in carboxymethylcellulose (CMC), hydroxyethylcellulose (HEC) and beta-glucan. Involved in the degradation of complex natural cellulosic substrates By similarity.By similarity

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei167 – 1671Proton donorBy similarity
    Active sitei213 – 2131NucleophileSequence Analysis

    GO - Molecular functioni

    1. cellulase activity Source: UniProtKB-EC
    2. cellulose binding Source: InterPro

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Protein family/group databases

    CAZyiCBM1. Carbohydrate-Binding Module Family 1.
    GH61. Glycoside Hydrolase Family 61.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable endo-beta-1,4-glucanase D (EC:3.2.1.4)
    Short name:
    Endoglucanase D
    Alternative name(s):
    Carboxymethylcellulase D
    Cellulase 61A
    Cellulase D
    Gene namesi
    Name:eglD
    Synonyms:cel61A
    ORF Names:AKAW_08531
    OrganismiAspergillus kawachii (strain NBRC 4308) (White koji mold) (Aspergillus awamori var. kawachi)
    Taxonomic identifieri1033177 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000006812: Unassembled WGS sequence

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Chaini21 – 408388Probable endo-beta-1,4-glucanase DPRO_0000394064Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi151 – 1511N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi331 – 3311N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi377 ↔ 394By similarity
    Glycosylationi381 – 3811N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi388 ↔ 404By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Structurei

    3D structure databases

    ProteinModelPortaliQ96WQ9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini369 – 40537CBM1PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni21 – 237217CatalyticAdd
    BLAST
    Regioni238 – 25417Ser/Thr-rich linkerAdd
    BLAST

    Domaini

    Has a modular structure: an endo-beta-1,4-glucanase catalytic module at the N-terminus, a linker rich in serines and threonines, and a C-terminal carbohydrate-binding module (CBM). The genes for catalytic modules and CBMs seem to have evolved separately and have been linked by gene fusion.

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 61 family.Curated
    Contains 1 CBM1 (fungal-type carbohydrate-binding) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    OrthoDBiEOG7KM64H.

    Family and domain databases

    InterProiIPR000254. Cellulose-bd_dom_fun.
    IPR005103. Glyco_hydro_61.
    [Graphical view]
    PfamiPF00734. CBM_1. 1 hit.
    PF03443. Glyco_hydro_61. 1 hit.
    [Graphical view]
    ProDomiPD001821. CBD_fun. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00236. fCBD. 1 hit.
    [Graphical view]
    SUPFAMiSSF57180. SSF57180. 1 hit.
    PROSITEiPS00562. CBM1_1. 1 hit.
    PS51164. CBM1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q96WQ9-1 [UniParc]FASTAAdd to Basket

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    MKTTTYSLLA LAAASKLASA HTTVQAVWIN GEDQGLGNTD DGYIRSPPSN    50
    SPVTDVTSTD MTCNVNGDQA ASKTLSVKAG DVVTFEWHHS DRSDSDDIIA 100
    SSHKGPVQVY MAPTAKGSNG NNWVKIAEDG YHKSSDEWAT DILIANKGKH 150
    NITVPDVPAG NYLFRPEIIA LHEGNREGGA QFYMECVQFK VTSDGSNELP 200
    SGVSIPGVYT ATDPGILFDI YNSFDSYPIP GPDVWDGSSS GSSSSGSSSA 250
    AVSSAAAAAT TSAVAATTPA TQAAVEVSSS AAAATTEAAA PVVSSAAPVQ 300
    QATSAVTSQA QAAPTTFATS SKKSSKTACK NKTKSNSQVA AATSSVVAPA 350
    ATSSVVPVVS ASASASAGGV AKQYERCGGI NHTGPTTCES GSVCKKWNPY 400
    YYQCVASQ 408
    Length:408
    Mass (Da):41,650
    Last modified:December 1, 2001 - v1
    Checksum:iB7CA86C9019F0089
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB055432 Genomic DNA. Translation: BAB62318.1.
    DF126473 Genomic DNA. Translation: GAA90417.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB055432 Genomic DNA. Translation: BAB62318.1 .
    DF126473 Genomic DNA. Translation: GAA90417.1 .

    3D structure databases

    ProteinModelPortali Q96WQ9.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM1. Carbohydrate-Binding Module Family 1.
    GH61. Glycoside Hydrolase Family 61.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    OrthoDBi EOG7KM64H.

    Family and domain databases

    InterProi IPR000254. Cellulose-bd_dom_fun.
    IPR005103. Glyco_hydro_61.
    [Graphical view ]
    Pfami PF00734. CBM_1. 1 hit.
    PF03443. Glyco_hydro_61. 1 hit.
    [Graphical view ]
    ProDomi PD001821. CBD_fun. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00236. fCBD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57180. SSF57180. 1 hit.
    PROSITEi PS00562. CBM1_1. 1 hit.
    PS51164. CBM1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequence analysis of endoglucanase genes from an industrial fungus, Aspergillus kawachii."
      Hara Y., Hinoki Y., Shimoi H., Ito K.
      Biosci. Biotechnol. Biochem. 67:2010-2013(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: NBRC 4308.
    2. "Genome sequence of the white koji mold Aspergillus kawachii IFO 4308, used for brewing the Japanese distilled spirit shochu."
      Futagami T., Mori K., Yamashita A., Wada S., Kajiwara Y., Takashita H., Omori T., Takegawa K., Tashiro K., Kuhara S., Goto M.
      Eukaryot. Cell 10:1586-1587(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NBRC 4308.

    Entry informationi

    Entry nameiEGLD_ASPKW
    AccessioniPrimary (citable) accession number: Q96WQ9
    Secondary accession number(s): G7XU08
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 18, 2010
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3