Q96WQ9 (EGLD_ASPKA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable endo-beta-1,4-glucanase D
Short name=Endoglucanase D
EC=3.2.1.4

Alternative name(s):
Carboxymethylcellulase D
Cellulase 61A
Cellulase D

Gene names

Name:	eglD
Synonyms:	cel61A

Organism

Aspergillus kawachii (White koji mold) (Aspergillus awamori var. kawachi)

Taxonomic identifier

40384 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	408 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Has endoglucanase activity on substrates containing beta-1,4 glycosidic bonds, like in carboxymethylcellulose (CMC), hydroxyethylcellulose (HEC) and beta-glucan. Involved in the degradation of complex natural cellulosic substrates By similarity.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted By similarity.
Domain	Has a modular structure: an endo-beta-1,4-glucanase catalytic module at the N-terminus, a linker rich in serines and threonines, and a C-terminal carbohydrate-binding module (CBM). The genes for catalytic modules and CBMs seem to have evolved separately and have been linked by gene fusion.
Sequence similarities	Belongs to the glycosyl hydrolase 61 family. Contains 1 CBM1 (fungal-type carbohydrate-binding) domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cellulase activity Inferred from electronic annotation. Source: EC cellulose binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 408	388	Probable endo-beta-1,4-glucanase D		PRO_0000394064
Regions
	Domain	369 – 405	37	CBM1
	Region	21 – 237	217	Catalytic
	Region	238 – 254	17	Ser/Thr-rich linker
Sites
	Active site	167	1	Proton donor By similarity
	Active site	213	1	Nucleophile Potential
Amino acid modifications
	Glycosylation	151	1	N-linked (GlcNAc...) Potential
	Glycosylation	331	1	N-linked (GlcNAc...) Potential
	Glycosylation	381	1	N-linked (GlcNAc...) Potential
	Disulfide bond	377 ↔ 394		By similarity
	Disulfide bond	388 ↔ 404		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q96WQ9 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: B7CA86C9019F0089	FASTA	408	41,650
	10 20 30 40 50 60 MKTTTYSLLA LAAASKLASA HTTVQAVWIN GEDQGLGNTD DGYIRSPPSN SPVTDVTSTD 70 80 90 100 110 120 MTCNVNGDQA ASKTLSVKAG DVVTFEWHHS DRSDSDDIIA SSHKGPVQVY MAPTAKGSNG 130 140 150 160 170 180 NNWVKIAEDG YHKSSDEWAT DILIANKGKH NITVPDVPAG NYLFRPEIIA LHEGNREGGA 190 200 210 220 230 240 QFYMECVQFK VTSDGSNELP SGVSIPGVYT ATDPGILFDI YNSFDSYPIP GPDVWDGSSS 250 260 270 280 290 300 GSSSSGSSSA AVSSAAAAAT TSAVAATTPA TQAAVEVSSS AAAATTEAAA PVVSSAAPVQ 310 320 330 340 350 360 QATSAVTSQA QAAPTTFATS SKKSSKTACK NKTKSNSQVA AATSSVVAPA ATSSVVPVVS 370 380 390 400 ASASASAGGV AKQYERCGGI NHTGPTTCES GSVCKKWNPY YYQCVASQ

References

[1]	"Cloning and sequence analysis of endoglucanase genes from an industrial fungus, Aspergillus kawachii." Hara Y., Hinoki Y., Shimoi H., Ito K. Biosci. Biotechnol. Biochem. 67:2010-2013(2003) [PubMed: 14519993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NBRC 4308.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB055432 Genomic DNA. Translation: BAB62318.1.
3D structure databases
ProteinModelPortal	Q96WQ9.
ModBase	Search...
Protein family/group databases
CAZy	CBM1. Carbohydrate-Binding Module Family 1. GH61. Glycoside Hydrolase Family 61.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000254. Cellulose-bd_dom_fun. IPR008972. Cupredoxin. IPR005103. Glyco_hydro_61. [Graphical view]
Gene3D	G3DSA:2.60.40.420. Cupredoxin. 1 hit.
Pfam	PF00734. CBM_1. 1 hit. PF03443. Glyco_hydro_61. 1 hit. [Graphical view]
ProDom	PD001821. CBD_fun. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00236. fCBD. 1 hit. [Graphical view]
SUPFAM	SSF57180. CBD_fun. 1 hit.
PROSITE	PS00562. CBM1_1. 1 hit. PS51164. CBM1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EGLD_ASPKA

Accession

Primary (citable) accession number: Q96WQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 18, 2010
Last sequence update:	December 1, 2001
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program