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Q96WQ8

- EGLB_ASPKW

UniProt

Q96WQ8 - EGLB_ASPKW

Protein

Probable endo-beta-1,4-glucanase B

Gene

eglB

Organism
Aspergillus kawachii (strain NBRC 4308) (White koji mold) (Aspergillus awamori var. kawachi)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Has endoglucanase activity on substrates containing beta-1,4 glycosidic bonds, like in carboxymethylcellulose (CMC), hydroxyethylcellulose (HEC) and beta-glucan. Involved in the degradation of complex natural cellulosic substrates By similarity.By similarity

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei160 – 1601Proton donorBy similarity
    Active sitei267 – 2671NucleophileBy similarity

    GO - Molecular functioni

    1. cellulase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Protein family/group databases

    CAZyiGH5. Glycoside Hydrolase Family 5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable endo-beta-1,4-glucanase B (EC:3.2.1.4)
    Short name:
    Endoglucanase B
    Alternative name(s):
    Carboxymethylcellulase B
    Cellulase 5B
    Cellulase B
    Gene namesi
    Name:eglB
    Synonyms:cel5B
    ORF Names:AKAW_03613
    OrganismiAspergillus kawachii (strain NBRC 4308) (White koji mold) (Aspergillus awamori var. kawachi)
    Taxonomic identifieri1033177 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000006812: Unassembled WGS sequence

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Chaini19 – 332314Probable endo-beta-1,4-glucanase BPRO_0000394056Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi38 – 381N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi100 – 1001N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi212 – 2121N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi289 – 2891N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Structurei

    3D structure databases

    ProteinModelPortaliQ96WQ8.
    SMRiQ96WQ8. Positions 31-332.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    OrthoDBiEOG776T0S.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001547. Glyco_hydro_5.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00150. Cellulase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q96WQ8-1 [UniParc]FASTAAdd to Basket

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    MKFQSTLLLA AAAGSALAVP HGPGHKKRAS VFEWFGSNES GAEFGTNIPG    50
    VWGTDYIFPD PSAISTLIDK GMNFFRVQFM MERLLPDSMT GSYDEEYLAN 100
    LTTVIKAVTD GGAHALVDPH NYGRYNGEII SSTSDFQTFW ENLAGQYKDN 150
    DLVMFDTNNE YHDMDQDLVL NLNQAAINGI RAAGATSQYI FVEGNSWTGA 200
    WTWVDVNDNM KNLTDPEDKI VYEMHQYLDS DGSGTSETCV SETIGKERVT 250
    EATQWLKDNK KVGFIGEYAG GSNDVCRSAV SGMLEYMANN TDVWKGASWW 300
    AAGPWWGDYI FSMEPPDGTA YTGMLDILEA YL 332
    Length:332
    Mass (Da):36,733
    Last modified:December 1, 2001 - v1
    Checksum:iA709672F5DEB1830
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB055433 Genomic DNA. Translation: BAB62319.1.
    DF126453 Genomic DNA. Translation: GAA85499.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB055433 Genomic DNA. Translation: BAB62319.1 .
    DF126453 Genomic DNA. Translation: GAA85499.1 .

    3D structure databases

    ProteinModelPortali Q96WQ8.
    SMRi Q96WQ8. Positions 31-332.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH5. Glycoside Hydrolase Family 5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    OrthoDBi EOG776T0S.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001547. Glyco_hydro_5.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00150. Cellulase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequence analysis of endoglucanase genes from an industrial fungus, Aspergillus kawachii."
      Hara Y., Hinoki Y., Shimoi H., Ito K.
      Biosci. Biotechnol. Biochem. 67:2010-2013(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: NBRC 4308.
    2. "Genome sequence of the white koji mold Aspergillus kawachii IFO 4308, used for brewing the Japanese distilled spirit shochu."
      Futagami T., Mori K., Yamashita A., Wada S., Kajiwara Y., Takashita H., Omori T., Takegawa K., Tashiro K., Kuhara S., Goto M.
      Eukaryot. Cell 10:1586-1587(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NBRC 4308.

    Entry informationi

    Entry nameiEGLB_ASPKW
    AccessioniPrimary (citable) accession number: Q96WQ8
    Secondary accession number(s): G7XEC0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 18, 2010
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3