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Q96WQ8

- EGLB_ASPKW

UniProt

Q96WQ8 - EGLB_ASPKW

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Protein

Probable endo-beta-1,4-glucanase B

Gene
eglB, cel5B, AKAW_03613
Organism
Aspergillus kawachii (strain NBRC 4308) (White koji mold) (Aspergillus awamori var. kawachi)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Has endoglucanase activity on substrates containing beta-1,4 glycosidic bonds, like in carboxymethylcellulose (CMC), hydroxyethylcellulose (HEC) and beta-glucan. Involved in the degradation of complex natural cellulosic substrates By similarity.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei160 – 1601Proton donor By similarity
Active sitei267 – 2671Nucleophile By similarity

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable endo-beta-1,4-glucanase B (EC:3.2.1.4)
Short name:
Endoglucanase B
Alternative name(s):
Carboxymethylcellulase B
Cellulase 5B
Cellulase B
Gene namesi
Name:eglB
Synonyms:cel5B
ORF Names:AKAW_03613
OrganismiAspergillus kawachii (strain NBRC 4308) (White koji mold) (Aspergillus awamori var. kawachi)
Taxonomic identifieri1033177 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000006812: Unassembled WGS sequence

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818 Reviewed predictionAdd
BLAST
Chaini19 – 332314Probable endo-beta-1,4-glucanase BPRO_0000394056Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi38 – 381N-linked (GlcNAc...) Reviewed prediction
Glycosylationi100 – 1001N-linked (GlcNAc...) Reviewed prediction
Glycosylationi212 – 2121N-linked (GlcNAc...) Reviewed prediction
Glycosylationi289 – 2891N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ96WQ8.
SMRiQ96WQ8. Positions 31-332.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

OrthoDBiEOG776T0S.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q96WQ8-1 [UniParc]FASTAAdd to Basket

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MKFQSTLLLA AAAGSALAVP HGPGHKKRAS VFEWFGSNES GAEFGTNIPG    50
VWGTDYIFPD PSAISTLIDK GMNFFRVQFM MERLLPDSMT GSYDEEYLAN 100
LTTVIKAVTD GGAHALVDPH NYGRYNGEII SSTSDFQTFW ENLAGQYKDN 150
DLVMFDTNNE YHDMDQDLVL NLNQAAINGI RAAGATSQYI FVEGNSWTGA 200
WTWVDVNDNM KNLTDPEDKI VYEMHQYLDS DGSGTSETCV SETIGKERVT 250
EATQWLKDNK KVGFIGEYAG GSNDVCRSAV SGMLEYMANN TDVWKGASWW 300
AAGPWWGDYI FSMEPPDGTA YTGMLDILEA YL 332
Length:332
Mass (Da):36,733
Last modified:December 1, 2001 - v1
Checksum:iA709672F5DEB1830
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB055433 Genomic DNA. Translation: BAB62319.1.
DF126453 Genomic DNA. Translation: GAA85499.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB055433 Genomic DNA. Translation: BAB62319.1 .
DF126453 Genomic DNA. Translation: GAA85499.1 .

3D structure databases

ProteinModelPortali Q96WQ8.
SMRi Q96WQ8. Positions 31-332.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

OrthoDBi EOG776T0S.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00150. Cellulase. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequence analysis of endoglucanase genes from an industrial fungus, Aspergillus kawachii."
    Hara Y., Hinoki Y., Shimoi H., Ito K.
    Biosci. Biotechnol. Biochem. 67:2010-2013(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: NBRC 4308.
  2. "Genome sequence of the white koji mold Aspergillus kawachii IFO 4308, used for brewing the Japanese distilled spirit shochu."
    Futagami T., Mori K., Yamashita A., Wada S., Kajiwara Y., Takashita H., Omori T., Takegawa K., Tashiro K., Kuhara S., Goto M.
    Eukaryot. Cell 10:1586-1587(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NBRC 4308.

Entry informationi

Entry nameiEGLB_ASPKW
AccessioniPrimary (citable) accession number: Q96WQ8
Secondary accession number(s): G7XEC0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 18, 2010
Last sequence update: December 1, 2001
Last modified: November 13, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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