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Q96WQ8 (EGLB_ASPKW) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable endo-beta-1,4-glucanase B

Short name=Endoglucanase B
EC=3.2.1.4
Alternative name(s):
Carboxymethylcellulase B
Cellulase 5B
Cellulase B
Gene names
Name:eglB
Synonyms:cel5B
ORF Names:AKAW_03613
OrganismAspergillus kawachii (strain NBRC 4308) (White koji mold) (Aspergillus awamori var. kawachi) [Complete proteome]
Taxonomic identifier1033177 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length332 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Has endoglucanase activity on substrates containing beta-1,4 glycosidic bonds, like in carboxymethylcellulose (CMC), hydroxyethylcellulose (HEC) and beta-glucan. Involved in the degradation of complex natural cellulosic substrates By similarity.

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Subcellular location

Secreted By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cellulose degradation
Polysaccharide degradation
   Cellular componentSecreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncellulase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 332314Probable endo-beta-1,4-glucanase B
PRO_0000394056

Sites

Active site1601Proton donor By similarity
Active site2671Nucleophile By similarity

Amino acid modifications

Glycosylation381N-linked (GlcNAc...) Potential
Glycosylation1001N-linked (GlcNAc...) Potential
Glycosylation2121N-linked (GlcNAc...) Potential
Glycosylation2891N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q96WQ8 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: A709672F5DEB1830

FASTA33236,733
        10         20         30         40         50         60 
MKFQSTLLLA AAAGSALAVP HGPGHKKRAS VFEWFGSNES GAEFGTNIPG VWGTDYIFPD 

        70         80         90        100        110        120 
PSAISTLIDK GMNFFRVQFM MERLLPDSMT GSYDEEYLAN LTTVIKAVTD GGAHALVDPH 

       130        140        150        160        170        180 
NYGRYNGEII SSTSDFQTFW ENLAGQYKDN DLVMFDTNNE YHDMDQDLVL NLNQAAINGI 

       190        200        210        220        230        240 
RAAGATSQYI FVEGNSWTGA WTWVDVNDNM KNLTDPEDKI VYEMHQYLDS DGSGTSETCV 

       250        260        270        280        290        300 
SETIGKERVT EATQWLKDNK KVGFIGEYAG GSNDVCRSAV SGMLEYMANN TDVWKGASWW 

       310        320        330 
AAGPWWGDYI FSMEPPDGTA YTGMLDILEA YL 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequence analysis of endoglucanase genes from an industrial fungus, Aspergillus kawachii."
Hara Y., Hinoki Y., Shimoi H., Ito K.
Biosci. Biotechnol. Biochem. 67:2010-2013(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: NBRC 4308.
[2]"Genome sequence of the white koji mold Aspergillus kawachii IFO 4308, used for brewing the Japanese distilled spirit shochu."
Futagami T., Mori K., Yamashita A., Wada S., Kajiwara Y., Takashita H., Omori T., Takegawa K., Tashiro K., Kuhara S., Goto M.
Eukaryot. Cell 10:1586-1587(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NBRC 4308.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB055433 Genomic DNA. Translation: BAB62319.1.
DF126453 Genomic DNA. Translation: GAA85499.1.

3D structure databases

ProteinModelPortalQ96WQ8.
SMRQ96WQ8. Positions 31-332.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

OrthoDBEOG776T0S.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameEGLB_ASPKW
AccessionPrimary (citable) accession number: Q96WQ8
Secondary accession number(s): G7XEC0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 18, 2010
Last sequence update: December 1, 2001
Last modified: November 13, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries