Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Laccase-2

Gene

lcc2

Organism
Botryotinia fuckeliana (Noble rot fungus) (Botrytis cinerea)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Lignin degradation and detoxification of lignin-derived products.By similarity

Catalytic activityi

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.

Cofactori

Cu cationBy similarityNote: Binds 4 Cu cations per monomer.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi125 – 1251Copper 1; type 2By similarity
Metal bindingi127 – 1271Copper 2; type 3By similarity
Metal bindingi169 – 1691Copper 2; type 3By similarity
Metal bindingi171 – 1711Copper 3; type 3By similarity
Metal bindingi464 – 4641Copper 4; type 1By similarity
Metal bindingi467 – 4671Copper 1; type 2By similarity
Metal bindingi469 – 4691Copper 3; type 3By similarity
Metal bindingi526 – 5261Copper 3; type 3By similarity
Metal bindingi527 – 5271Copper 4; type 1By similarity
Metal bindingi528 – 5281Copper 2; type 3By similarity
Metal bindingi532 – 5321Copper 4; type 1By similarity

GO - Molecular functioni

  1. copper ion binding Source: InterPro
  2. hydroquinone:oxygen oxidoreductase activity Source: UniProtKB-EC

GO - Biological processi

  1. lignin catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lignin degradation

Keywords - Ligandi

Copper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Laccase-2 (EC:1.10.3.2)
Alternative name(s):
Benzenediol:oxygen oxidoreductase 2
Diphenol oxidase 2
Urishiol oxidase 2
Gene namesi
Name:lcc2
OrganismiBotryotinia fuckeliana (Noble rot fungus) (Botrytis cinerea)
Taxonomic identifieri40559 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaLeotiomycetesHelotialesSclerotiniaceaeBotrytis

Subcellular locationi

Secreted Curated

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 581562Laccase-2PRO_0000267638Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi77 – 771N-linked (GlcNAc...)Sequence Analysis
Glycosylationi93 – 931N-linked (GlcNAc...)Sequence Analysis
Glycosylationi120 – 1201N-linked (GlcNAc...)Sequence Analysis
Glycosylationi232 – 2321N-linked (GlcNAc...)Sequence Analysis
Glycosylationi283 – 2831N-linked (GlcNAc...)Sequence Analysis
Glycosylationi343 – 3431N-linked (GlcNAc...)Sequence Analysis
Glycosylationi408 – 4081N-linked (GlcNAc...)Sequence Analysis
Glycosylationi427 – 4271N-linked (GlcNAc...)Sequence Analysis
Glycosylationi441 – 4411N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Expressioni

Inductioni

By resveratrol and tannins.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ96WM9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini74 – 191118Plastocyanin-like 1Add
BLAST
Domaini197 – 353157Plastocyanin-like 2Add
BLAST
Domaini413 – 547135Plastocyanin-like 3Add
BLAST

Sequence similaritiesi

Belongs to the multicopper oxidase family.Curated
Contains 3 plastocyanin-like domains.Curated

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.60.40.420. 3 hits.
InterProiIPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
[Graphical view]
PfamiPF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 3 hits.
PROSITEiPS00079. MULTICOPPER_OXIDASE1. 1 hit.
PS00080. MULTICOPPER_OXIDASE2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q96WM9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKYSTVFTAL TALFAQASAT AIPAVRSPLA PRQSTTASCA NSATSRSCWG
60 70 80 90 100
EYSIDTNWYD VTPNTGVTRE YWLSVENSTI TPDGYTRSAM TFNGTVPGPA
110 120 130 140 150
ITADWGDNLI IHVTNNLQHN GTSIHWHGIR QLGSLEYDGV PGVTQCPIAP
160 170 180 190 200
GDTLTYKFQA TQYGTTWYHS HFSLQYADGL FGPLIINGPA TADYDEDVGA
210 220 230 240 250
IFLQDWAHKS VFEIWDSARQ GAPPALENTL MNGTNIYDCS ASTDANCVGG
260 270 280 290 300
GKKFELTFVE GTKYRLRLIN VGIDSHFEFA IDNHTLTVIA NDLVPIVPYT
310 320 330 340 350
TDTLLIGIGQ RYDVIVEANA AADNYWIRGN WGTTCSSNSE AANATGILRY
360 370 380 390 400
DSSSTVDPTS VGVTPRGTCA DEPVASLVPH LALDVGGYSL VDEQVSFAFT
410 420 430 440 450
NYFTWTINSS SLLLDWSSPT TLKIFNNETI FPTDYNVVAL NQTDANEEWV
460 470 480 490 500
VYVIEDLTGF GIWHPIHLHG HDFYVVAQET DVFSATKSPA NFNLVNPPRR
510 520 530 540 550
DVAALPGNGY LAIAFKLDNP GSWLLHCHIA WHASEGLAMQ FVESQSSIAI
560 570 580
GMSDTDIFED TCANWNAYTP TELFAEDDSG I
Length:581
Mass (Da):63,434
Last modified:December 1, 2001 - v1
Checksum:i674947DAFD6BC757
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF243855 Genomic DNA. Translation: AAK77953.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF243855 Genomic DNA. Translation: AAK77953.1.

3D structure databases

ProteinModelPortaliQ96WM9.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.60.40.420. 3 hits.
InterProiIPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
[Graphical view]
PfamiPF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 3 hits.
PROSITEiPS00079. MULTICOPPER_OXIDASE1. 1 hit.
PS00080. MULTICOPPER_OXIDASE2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Resveratrol acts as a natural profungicide and induces self-intoxication by a specific laccase."
    Schouten A., Wagemakers L., Stefanato F.L., van der Kaaij R.M., van Kan J.A.L.
    Mol. Microbiol. 43:883-894(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
    Strain: SAS56.

Entry informationi

Entry nameiLAC2_BOTFU
AccessioniPrimary (citable) accession number: Q96WM9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: December 1, 2001
Last modified: January 7, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.