Q96VT7 (DAPB_ASPNG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dipeptidyl-aminopeptidase B
Short name=DPAP B
EC=3.4.14.5

Gene names

Name:

dapB

Organism

Aspergillus niger

Taxonomic identifier

5061 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	901 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Type IV dipeptidyl-peptidase which removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. Ref.1
Catalytic activity	Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.
Subcellular location	Vacuole membrane; Single-pass type II membrane protein By similarity. Note: Lysosome-like vacuoles By similarity.
Sequence similarities	Belongs to the peptidase S9B family.

Ontologies

Keywords
Cellular component	Membrane Vacuole
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Aminopeptidase Hydrolase Protease Serine protease
PTM	Glycoprotein
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW vacuolar membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW serine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 901	901	Dipeptidyl-aminopeptidase B		PRO_0000412137
Regions
	Topological domain	1 – 76	76	Cytoplasmic Potential
	Transmembrane	77 – 97	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	98 – 901	804	Vacuolar Potential
Sites
	Active site	739	1	Charge relay system By similarity
	Active site	816	1	Charge relay system By similarity
	Active site	849	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	334	1	N-linked (GlcNAc...) Potential
	Glycosylation	625	1	N-linked (GlcNAc...) Potential
	Glycosylation	793	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q96VT7 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 3A519512C903D784	FASTA	901	101,256
	10 20 30 40 50 60 MSSPRPSTSS TSSDSGLSVD TTAYPEESKY TSTAPGAGGL SDENRYRDVE EGEAGADEPF 70 80 90 100 110 120 LPSAKKQAAS GSRTSRLIWG LVILCVAGWL WGLVLFVTQN RSAQQSVSEA LQSHESGAIS 130 140 150 160 170 180 GSSSSGKPVT LEQVLTGQWL PRSHAVSWIA GPNGEDGLLV EQGEDQGKGY LRVDDIRSRK 190 200 210 220 230 240 GDATSQESRV LMEKAIVQVD GRTIFPVSTW PSPNLNKVLL LSEREKNWRH SFTGKYWIFD 250 260 270 280 290 300 VATQTAQPLD PSNPDGRVQL AIWSPTSDMV AFVRDNNLYL RRLSSKEVVP ITKDGGADLF 310 320 330 340 350 360 YGIPDWVYEE EVFSGNSVTW WSGDGKYVAF LRTNETAVPE FPVQYYLSRP SGKRPPPGLE 370 380 390 400 410 420 DYPEVREIKY PKAGAPNPVV SLQFYDVEKQ EVFSIEAPDD FEDDDRIVIE IVWGTEGKIL 430 440 450 460 470 480 VRATNRESDV LKVFLFDTKA RTSKLVRTEN VADIDGGWVE PTQYTWFIPA DPSNGRPHDG 490 500 510 520 530 540 YLDTVIHEGY EHLGYFTPLD NSEPILLTQG EWEVVDAPTA VDLRKGIVYF ISTKESPTER 550 560 570 580 590 600 HLYQVNLDGS NLKPLTDTSK PGYYDVSFSH GTGYALLSYR GPSIPWQAIV NTETDELKYE 610 620 630 640 650 660 ETIEDNAGLA RMVDSYALPT EIYQNVTIDG FTLQVVERRP PHFNPAKKYP VLFYLYNGPR 670 680 690 700 710 720 SQTVDRKFSI DFQSYVASSL GYIVVTVDGR GTGFSGRKTR CIVRGNLGYY EAYDQITTAN 730 740 750 760 770 780 LWGEKPYVDE TRMSIWGWSY GGFMTLKTLE QDAGQTFQYG MAVAPVTDWR HYDSIYTERY 790 800 810 820 830 840 MHTPAHNPNG YDNTSITDMT ALQQTVRFLV IHGASDDNVH IQNTLVLVDK LDLAGVQNYD 850 860 870 880 890 900 LHFYPDSDHS INFHNAHRMV YERLSSWLVN AFNDEWHRIA DPVPDDSMWE KVKRSLPMLV N

References

[1]

"Characterisation of the Aspergillus niger dapB gene, which encodes a novel fungal type IV dipeptidyl aminopeptidase." Jalving R., Godefrooij J., Veen W.J., van Ooyen A.J., Schaap P.J. Mol. Genet. Genomics 273:319-325(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: ATCC 9029 / NRRL 3 / CBS 120.49 / DSM 2466 / N400.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ278532 Genomic DNA. Translation: CAC41019.1.
3D structure databases
HSSP	HSSP built from PDB template 1PFQ based on UniProtKB P27487.
ProteinModelPortal	Q96VT7.
ModBase	Search...
Protein family/group databases
MEROPS	S09.006.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	COG1506.
Enzyme and pathway databases
BRENDA	3.4.14.5. 277.
Family and domain databases
InterPro	IPR001375. Peptidase_S9. IPR002469. Peptidase_S9B. [Graphical view]
Pfam	PF00930. DPPIV_N. 1 hit. PF00326. Peptidase_S9. 1 hit. [Graphical view]
PROSITE	PS00708. PRO_ENDOPEP_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPB_ASPNG

Accession

Primary (citable) accession number: Q96VT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 27, 2011
Last sequence update:	December 1, 2001
Last modified:	April 3, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program