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Protein

Enolase

Gene
N/A
Organism
Cochliobolus lunatus (Filamentous fungus) (Curvularia lunata)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (GPD)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Enolase
  5. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei159SubstrateBy similarity1
Binding sitei168SubstrateBy similarity1
Active sitei211Proton donorBy similarity1
Metal bindingi246MagnesiumBy similarity1
Metal bindingi297MagnesiumBy similarity1
Binding sitei297SubstrateBy similarity1
Metal bindingi322MagnesiumBy similarity1
Binding sitei322SubstrateBy similarity1
Active sitei347Proton acceptorBy similarity1
Binding sitei398SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BRENDAi4.2.1.11. 1762.
UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase
2-phosphoglycerate dehydratase
OrganismiCochliobolus lunatus (Filamentous fungus) (Curvularia lunata)
Taxonomic identifieri5503 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaDothideomycetesPleosporomycetidaePleosporalesPleosporineaePleosporaceaeCurvularia

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Protein family/group databases

Allergomei2935. Cur l 2.
3571. Cur l 2.0101.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001340491 – 440EnolaseAdd BLAST440

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ96VP4.
SMRiQ96VP4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni374 – 377Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Curated

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q96VP4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAITKIHARS VYDSRGNPTV EVDIVTETGL HRAIVPSGAS TGSHEACELR
60 70 80 90 100
DGDKTKWGGK GVTKAVANVN DIIAPALIKE KLDVKDQSAV DAFLNKLDGT
110 120 130 140 150
ENKTKLGANA ILGVSMAIAK AAAAEKGVPL YAHISDLAGT KKPYVFRFLS
160 170 180 190 200
KNVLNGGSHA GGFLLFQEFM IAPAKTFAEA LRIRQGAEVY QKLKALTKKT
210 220 230 240 250
YGQSAGNYGD EGGVAPDIQT AEEALDLIVD AIEAAGHTGQ IKIAMDVASS
260 270 280 290 300
EFFKDDEKKY DLDFKNPDSD KSKWLTYPQL AEMYKSLAEK YPIVSIEDPF
310 320 330 340 350
AEDDWEAWSH FYKDGDFQIV GDDLTVTNPE FIKTAIELKS CNALLLKVNQ
360 370 380 390 400
IGTISEAINA AKDAFGAGWG VMVSHRSGET EDVTIADIVV GLRSGQIKTN
410 420 430 440
APARSERLAK YNQILRIEEE LGDKRLFAGN KFHTAINLYL
Length:440
Mass (Da):47,793
Last modified:December 1, 2001 - v1
Checksum:i3907350CE8FF082A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY034826 mRNA. Translation: AAK67491.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY034826 mRNA. Translation: AAK67491.1.

3D structure databases

ProteinModelPortaliQ96VP4.
SMRiQ96VP4.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei2935. Cur l 2.
3571. Cur l 2.0101.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.
BRENDAi4.2.1.11. 1762.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiENO_COCLU
AccessioniPrimary (citable) accession number: Q96VP4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 1, 2001
Last modified: March 15, 2017
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.