Reviewed, UniProtKB/Swiss-Prot Q96VJ7 (DHE4_GIBFU)

Names and origin

Protein names

Recommended name:
    NADP-specific glutamate dehydrogenase
      Short name=NADP-GDH
    EC=1.4.1.4
Alternative name(s):
    NADP-dependent glutamate dehydrogenase

Gene names

Name:

GDH2

Organism

Gibberella fujikuroi (Bakanae and foot rot disease fungus) (Fusarium moniliforme)

Taxonomic identifier

5127 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › Nectriaceae › Gibberella › Gibberella fujikuroi complex

Protein attributes

Sequence length	451 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.
Subunit structure	Homohexamer By similarity.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 451	451	NADP-specific glutamate dehydrogenase		PRO_0000182787
Sites
	Active site	114	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q96VJ7-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 171867950A9B8E85	FASTA	451	48,826
	10 20 30 40 50 60 MSHLPQEPEF EQAYGELASA LENSSLFNEH PEYRTALAVA AIPERVIQFR VVWNDDKGNL 70 80 90 100 110 120 QVNRGYRVQF NGALGPYKGG LRFHPSVNLS ILKFLGFEQI FKNALTGLNM GGGKGGADFD 130 140 150 160 170 180 PKGKSDAEIR RFCQAFMTEL SKHIGAETDV PAGDIGVGGR EIGYLFGAYR KFANRWEGVL 190 200 210 220 230 240 TGKGLSWGGS LIRPEATGYG LVYYVEYMLK HANRGTFEGK RVALSGSGNV AQYAALKIIE 250 260 270 280 290 300 LGGSVVSLSD SKGALVAKEG SSFTPEQIHN IAALKIKHQA LTTFEHDGQF TWIEGARPWV 310 320 330 340 350 360 HVGKVDIALP SATQNEVSKE EAQALVDAGA FIVAEGSNMG CTAEAIDVFE AHRKEKGAEA 370 380 390 400 410 420 LWYAPGKASN CGGVAVSGLE MAQNSQRIQW TEKEVDDRLK AIMKDAFVAG LETAQKYVEA 430 440 450 KEGELPSLIA GSNIAGFIKV AEAMHDQGDW F

References

[1]	"Cloning and characterization of the NADP-depending glutamate dehydrogenase gene from Gibberella fujikuroi." Tudzynski B. Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: m567.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ315471 Genomic DNA. Translation: CAC45043.1.
3D structure databases
SMR	Q96VJ7. Positions 4-450.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.4.1.4. 97815.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom. IPR014362. Glu_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
SMART	SM00839. ELFV_dehydrog. 1 hit. [Graphical view]
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE4_GIBFU

Accession

Primary (citable) accession number: Q96VJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	December 1, 2001
Last modified:	January 19, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)