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Q96VB6

- XYNF3_ASPOR

UniProt

Q96VB6 - XYNF3_ASPOR

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Protein

Endo-1,4-beta-xylanase F3

Gene
xynF3, xlnF3, AO090001000208
Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Endo-1,4-beta-xylanase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose.1 Publication

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.

Kineticsi

  1. KM=200 µM for birch wood xylan1 Publication

Vmax=435 µmol/min/mg enzyme

pH dependencei

Optimum pH is 5.5. Retain 85 percent of its activity in the pH range from 6.0 to 7.0 after a 12 h incubation at 25 degrees Celsius.

Temperature dependencei

Optimum temperature is 58 degrees Celsius. The purified enzyme showed complete stability under 60 degrees Celsius.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei153 – 1531Proton donor By similarity
Active sitei259 – 2591Nucleophile By similarity

GO - Molecular functioni

  1. endo-1,4-beta-xylanase activity Source: UniProtKB

GO - Biological processi

  1. xylan catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation, Xylan degradation

Enzyme and pathway databases

UniPathwayiUPA00114.

Protein family/group databases

CAZyiGH10. Glycoside Hydrolase Family 10.
mycoCLAPiXYN10C_ASPOR.

Names & Taxonomyi

Protein namesi
Recommended name:
Endo-1,4-beta-xylanase F3 (EC:3.2.1.8)
Short name:
Xylanase F3
Alternative name(s):
1,4-beta-D-xylan xylanohydrolase F3
Gene namesi
Name:xynF3
Synonyms:xlnF3
ORF Names:AO090001000208
OrganismiAspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Taxonomic identifieri510516 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000006564: Chromosome 2

Subcellular locationi

Secreted 2 Publications

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323 Reviewed predictionAdd
BLAST
Chaini24 – 323300Endo-1,4-beta-xylanase F3PRO_0000393192Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi277 ↔ 283 By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi5062.CADAORAP00000185.

Structurei

3D structure databases

ProteinModelPortaliQ96VB6.
SMRiQ96VB6. Positions 23-323.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000019847.
OrthoDBiEOG7GJ6PM.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001000. Glyco_hydro_10.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00331. Glyco_hydro_10. 1 hit.
[Graphical view]
PRINTSiPR00134. GLHYDRLASE10.
SMARTiSM00633. Glyco_10. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q96VB6-1 [UniParc]FASTAAdd to Basket

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MVHLKSLAGI LLYTSLCIAS SQQAPASINN AFVAKGKKYF GTCADQGTLS    50
DGTNSGIIKA DFGQLTPENS MKWDATEPSQ GKFSFSGADY LVNYAATNNK 100
LIRGHTLVWH SQLPSWVQGI TDKNTLTSVL KNHITTVMNR YKGKVYAWDV 150
VNEIFNEDGT LRSSVFYNVL GEDFVRIAFE TARAADPQAK LYINDYNLDS 200
ANYGKTTGLA NHVKKWIAQG IPIDGIGSQT HLSAGGSSGV KGALNTLAAS 250
GVSEVAITEL DIAGASSNDY VNVVEACLEV SKCVGITVWG VSDKNSWRSA 300
ESPLLFDGNY QPKSAYNAIL NAL 323
Length:323
Mass (Da):34,696
Last modified:December 1, 2001 - v1
Checksum:i474B2F7E130AD465
GO

Sequence cautioni

The sequence BAE56751.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EU848306 mRNA. Translation: ACJ26383.1.
AB066176 Genomic DNA. Translation: BAB69073.1.
AP007154 Genomic DNA. Translation: BAE56751.1. Sequence problems.
PIRiJC7813.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EU848306 mRNA. Translation: ACJ26383.1 .
AB066176 Genomic DNA. Translation: BAB69073.1 .
AP007154 Genomic DNA. Translation: BAE56751.1 . Sequence problems.
PIRi JC7813.

3D structure databases

ProteinModelPortali Q96VB6.
SMRi Q96VB6. Positions 23-323.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5062.CADAORAP00000185.

Protein family/group databases

CAZyi GH10. Glycoside Hydrolase Family 10.
mycoCLAPi XYN10C_ASPOR.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOGENOMi HOG000019847.
OrthoDBi EOG7GJ6PM.

Enzyme and pathway databases

UniPathwayi UPA00114 .

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001000. Glyco_hydro_10.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00331. Glyco_hydro_10. 1 hit.
[Graphical view ]
PRINTSi PR00134. GLHYDRLASE10.
SMARTi SM00633. Glyco_10. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning, characterization, and expression analysis of the xynF3 gene from Aspergillus oryzae."
    Kimura T., Suzuki H., Furuhashi H., Aburatani T., Morimoto K., Sakka K., Ohmiya K.
    Biosci. Biotechnol. Biochem. 66:285-292(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES.
  2. "Gene cloning, sequencing, expression and characterization of a xylanase gene from Aspergillus niger DMS1957."
    Quyen D.T., Nguyen S.L.T.
    Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: VTCC-F-187.
  3. "Genome sequencing and analysis of Aspergillus oryzae."
    Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.
    , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H.
    Nature 438:1157-1161(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 42149 / RIB 40.
  4. "Proteomic analysis of extracellular proteins from Aspergillus oryzae grown under submerged and solid-state culture conditions."
    Oda K., Kakizono D., Yamada O., Iefuji H., Akita O., Iwashita K.
    Appl. Environ. Microbiol. 72:3448-3457(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiXYNF3_ASPOR
AccessioniPrimary (citable) accession number: Q96VB6
Secondary accession number(s): Q2UNW4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 23, 2010
Last sequence update: December 1, 2001
Last modified: February 19, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

The promoter does not contain any 5'-GGCTAAA-3' sequence identified as binding sites for the xylanolytic transcriptional activator xlnR.

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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