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Q96VB6

- XYNF3_ASPOR

UniProt

Q96VB6 - XYNF3_ASPOR

Protein

Endo-1,4-beta-xylanase F3

Gene

xynF3

Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Endo-1,4-beta-xylanase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose.1 Publication

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.

    Kineticsi

    1. KM=200 µM for birch wood xylan1 Publication

    Vmax=435 µmol/min/mg enzyme1 Publication

    pH dependencei

    Optimum pH is 5.5. Retain 85 percent of its activity in the pH range from 6.0 to 7.0 after a 12 h incubation at 25 degrees Celsius.1 Publication

    Temperature dependencei

    Optimum temperature is 58 degrees Celsius. The purified enzyme showed complete stability under 60 degrees Celsius.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei153 – 1531Proton donorBy similarity
    Active sitei259 – 2591NucleophileBy similarity

    GO - Molecular functioni

    1. endo-1,4-beta-xylanase activity Source: UniProtKB

    GO - Biological processi

    1. xylan catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation, Xylan degradation

    Enzyme and pathway databases

    UniPathwayiUPA00114.

    Protein family/group databases

    CAZyiGH10. Glycoside Hydrolase Family 10.
    mycoCLAPiXYN10C_ASPOR.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endo-1,4-beta-xylanase F3 (EC:3.2.1.8)
    Short name:
    Xylanase F3
    Alternative name(s):
    1,4-beta-D-xylan xylanohydrolase F3
    Gene namesi
    Name:xynF3
    Synonyms:xlnF3
    ORF Names:AO090001000208
    OrganismiAspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
    Taxonomic identifieri510516 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000006564: Chromosome 2

    Subcellular locationi

    Secreted 2 Publications

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 323300Endo-1,4-beta-xylanase F3PRO_0000393192Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi277 ↔ 283By similarity

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi5062.CADAORAP00000185.

    Structurei

    3D structure databases

    ProteinModelPortaliQ96VB6.
    SMRiQ96VB6. Positions 23-323.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOGENOMiHOG000019847.
    OrthoDBiEOG7GJ6PM.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001000. Glyco_hydro_10.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00331. Glyco_hydro_10. 1 hit.
    [Graphical view]
    PRINTSiPR00134. GLHYDRLASE10.
    SMARTiSM00633. Glyco_10. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q96VB6-1 [UniParc]FASTAAdd to Basket

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    MVHLKSLAGI LLYTSLCIAS SQQAPASINN AFVAKGKKYF GTCADQGTLS    50
    DGTNSGIIKA DFGQLTPENS MKWDATEPSQ GKFSFSGADY LVNYAATNNK 100
    LIRGHTLVWH SQLPSWVQGI TDKNTLTSVL KNHITTVMNR YKGKVYAWDV 150
    VNEIFNEDGT LRSSVFYNVL GEDFVRIAFE TARAADPQAK LYINDYNLDS 200
    ANYGKTTGLA NHVKKWIAQG IPIDGIGSQT HLSAGGSSGV KGALNTLAAS 250
    GVSEVAITEL DIAGASSNDY VNVVEACLEV SKCVGITVWG VSDKNSWRSA 300
    ESPLLFDGNY QPKSAYNAIL NAL 323
    Length:323
    Mass (Da):34,696
    Last modified:December 1, 2001 - v1
    Checksum:i474B2F7E130AD465
    GO

    Sequence cautioni

    The sequence BAE56751.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EU848306 mRNA. Translation: ACJ26383.1.
    AB066176 Genomic DNA. Translation: BAB69073.1.
    AP007154 Genomic DNA. Translation: BAE56751.1. Sequence problems.
    PIRiJC7813.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EU848306 mRNA. Translation: ACJ26383.1 .
    AB066176 Genomic DNA. Translation: BAB69073.1 .
    AP007154 Genomic DNA. Translation: BAE56751.1 . Sequence problems.
    PIRi JC7813.

    3D structure databases

    ProteinModelPortali Q96VB6.
    SMRi Q96VB6. Positions 23-323.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5062.CADAORAP00000185.

    Protein family/group databases

    CAZyi GH10. Glycoside Hydrolase Family 10.
    mycoCLAPi XYN10C_ASPOR.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOGENOMi HOG000019847.
    OrthoDBi EOG7GJ6PM.

    Enzyme and pathway databases

    UniPathwayi UPA00114 .

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001000. Glyco_hydro_10.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00331. Glyco_hydro_10. 1 hit.
    [Graphical view ]
    PRINTSi PR00134. GLHYDRLASE10.
    SMARTi SM00633. Glyco_10. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning, characterization, and expression analysis of the xynF3 gene from Aspergillus oryzae."
      Kimura T., Suzuki H., Furuhashi H., Aburatani T., Morimoto K., Sakka K., Ohmiya K.
      Biosci. Biotechnol. Biochem. 66:285-292(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES.
    2. "Gene cloning, sequencing, expression and characterization of a xylanase gene from Aspergillus niger DMS1957."
      Quyen D.T., Nguyen S.L.T.
      Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: VTCC-F-187.
    3. "Genome sequencing and analysis of Aspergillus oryzae."
      Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.
      , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H.
      Nature 438:1157-1161(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 42149 / RIB 40.
    4. "Proteomic analysis of extracellular proteins from Aspergillus oryzae grown under submerged and solid-state culture conditions."
      Oda K., Kakizono D., Yamada O., Iefuji H., Akita O., Iwashita K.
      Appl. Environ. Microbiol. 72:3448-3457(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiXYNF3_ASPOR
    AccessioniPrimary (citable) accession number: Q96VB6
    Secondary accession number(s): Q2UNW4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 23, 2010
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The promoter does not contain any 5'-GGCTAAA-3' sequence identified as binding sites for the xylanolytic transcriptional activator xlnR.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3