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Protein

1,4-alpha-glucan-branching enzyme

Gene

gbeA

Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathwayi: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei345NucleophileBy similarity1
Active sitei400Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processGlycogen biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan-branching enzyme (EC:2.4.1.18)
Alternative name(s):
Glycogen-branching enzyme
Gene namesi
Name:gbeA
ORF Names:AO090010000483
OrganismiAspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Taxonomic identifieri510516 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006564 Componenti: Chromosome 8

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001887781 – 6891,4-alpha-glucan-branching enzymeAdd BLAST689

Interactioni

Protein-protein interaction databases

STRINGi5062.CADAORAP00004462.

Structurei

3D structure databases

ProteinModelPortaliQ96VA4.
SMRiQ96VA4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000175159.
KOiK00700.
OMAiEVVHGKS.
OrthoDBiEOG092C0QFK.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiView protein in InterPro
IPR006048. A-amylase/branching_C.
IPR006407. GlgB.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
PfamiView protein in Pfam
PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
PIRSFiPIRSF000463. GlgB. 1 hit.
SMARTiView protein in SMART
SM00642. Aamy. 1 hit.
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

Q96VA4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTSQAVDSS PPDGTGVIQL DPWLEPFRDA LKQRFSFIEG WVKAINETEG
60 70 80 90 100
GLETFSKGYE RFGLNVQSNG DIIYREWAPN AVQAQLVGEF NNWDVTAHPM
110 120 130 140 150
TKNGFGVWEV TVPAVNGAPA IPHDSKIKIS MVIPSGERIY RIPAWIKRVV
160 170 180 190 200
QDLSVSPTYE AVFWNPPTEK QYKFQYSRPK RPESLRIYEA HVGISSPETK
210 220 230 240 250
VATYKEFTSN MLPRIKYLGY NAIQLMAIME HAYYASFGYQ VNNFFAASSR
260 270 280 290 300
YGTPEDLKEL VDKAHSMGLV VLLDVVHSHA SKNVLDGLNM FDGTDHLYFH
310 320 330 340 350
GGGKGRHELW DSRLFNYGHH EVLRFLLSNL RFWMEEYGFD GFRFDGVTSM
360 370 380 390 400
LYTHHGIGTG FSGGYHEYFG SSVDEEGVMY LTLANEMLHN LYPNCITVAE
410 420 430 440 450
DVSGMPALCL PHSLGGVGFD YRLAMAVPDM YIKLLKEKKD DEWDIGNLSF
460 470 480 490 500
TLTNRRHGEK TIAYAESHDQ ALVGDKTLMM WLCDKEMYTH MSVLTEFTPI
510 520 530 540 550
IERGMALHKL IRLVTHGLGG EGYLNFEGNE FGHPEWLDFP RDGNNNSFWY
560 570 580 590 600
ARRQLNLTED HLLRYKFLND FDRAMQLTEE KYGWLHSPQA YVSLKNETDK
610 620 630 640 650
VLVFERAGLL WIFNFHPTNS FTDYRVGVEQ SGTYRIVLDT DDPAFGGLNR
660 670 680
NLKETRFFTT DLSWNGRSNF LQVYIPTRTA LVLALEETL
Length:689
Mass (Da):78,751
Last modified:December 1, 2001 - v1
Checksum:i2B478A5334064432
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB072730 mRNA. Translation: BAB69770.1.
AP007175 Genomic DNA. Translation: BAE66334.1.
RefSeqiXP_001827467.1. XM_001827415.2.

Genome annotation databases

EnsemblFungiiBAE66334; BAE66334; AO090010000483.
GeneIDi5999601.
KEGGiaor:AOR_1_796024.

Similar proteinsi

Entry informationi

Entry nameiGLGB_ASPOR
AccessioniPrimary (citable) accession number: Q96VA4
Secondary accession number(s): Q2TWN9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: December 1, 2001
Last modified: September 27, 2017
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families