Reviewed, UniProtKB/Swiss-Prot Q96VA4 (GLGB_ASPOR)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme

Gene names

Name:	gbeA
ORF Names:	AO090010000483

Organism

Aspergillus oryzae [Complete proteome]

Taxonomic identifier

5062 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	689 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.
Pathway	Glycan biosynthesis; glycogen biosynthesis.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 689	689	1,4-alpha-glucan-branching enzyme		PRO_0000188778
Sites
	Active site	239	1	By similarity
	Active site	274	1	By similarity
	Active site	279	1	By similarity
	Active site	343	1	By similarity
	Active site	345	1	By similarity
	Active site	400	1	By similarity
	Active site	468	1	By similarity
	Active site	469	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q96VA4-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 2B478A5334064432	FASTA	689	78,751
	10 20 30 40 50 60 MGTSQAVDSS PPDGTGVIQL DPWLEPFRDA LKQRFSFIEG WVKAINETEG GLETFSKGYE 70 80 90 100 110 120 RFGLNVQSNG DIIYREWAPN AVQAQLVGEF NNWDVTAHPM TKNGFGVWEV TVPAVNGAPA 130 140 150 160 170 180 IPHDSKIKIS MVIPSGERIY RIPAWIKRVV QDLSVSPTYE AVFWNPPTEK QYKFQYSRPK 190 200 210 220 230 240 RPESLRIYEA HVGISSPETK VATYKEFTSN MLPRIKYLGY NAIQLMAIME HAYYASFGYQ 250 260 270 280 290 300 VNNFFAASSR YGTPEDLKEL VDKAHSMGLV VLLDVVHSHA SKNVLDGLNM FDGTDHLYFH 310 320 330 340 350 360 GGGKGRHELW DSRLFNYGHH EVLRFLLSNL RFWMEEYGFD GFRFDGVTSM LYTHHGIGTG 370 380 390 400 410 420 FSGGYHEYFG SSVDEEGVMY LTLANEMLHN LYPNCITVAE DVSGMPALCL PHSLGGVGFD 430 440 450 460 470 480 YRLAMAVPDM YIKLLKEKKD DEWDIGNLSF TLTNRRHGEK TIAYAESHDQ ALVGDKTLMM 490 500 510 520 530 540 WLCDKEMYTH MSVLTEFTPI IERGMALHKL IRLVTHGLGG EGYLNFEGNE FGHPEWLDFP 550 560 570 580 590 600 RDGNNNSFWY ARRQLNLTED HLLRYKFLND FDRAMQLTEE KYGWLHSPQA YVSLKNETDK 610 620 630 640 650 660 VLVFERAGLL WIFNFHPTNS FTDYRVGVEQ SGTYRIVLDT DDPAFGGLNR NLKETRFFTT 670 680 DLSWNGRSNF LQVYIPTRTA LVLALEETL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural features of the glycogen branching enzyme encoding genes from aspergilli." Sasangka P., Matsuno A., Tanaka A., Akasaka Y., Suyama S., Kano S., Miyazaki M., Akao T., Kato M., Kobayashi T., Tsukagoshi N. Microbiol. Res. 157:337-344(2002) [PubMed: 12501999] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ATCC 42149 / RIB 40.
[2]	"Genome sequencing and analysis of Aspergillus oryzae." Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E. , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H. Nature 438:1157-1161(2005) [PubMed: 16372010] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 42149 / RIB 40.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB072730 mRNA. Translation: BAB69770.1. AP007175 Genomic DNA. Translation: BAE66334.1.
RefSeq	XP_001827467.1.
3D structure databases
HSSP	HSSP built from PDB template 1M7X based on UniProtKB P07762.
ModBase	Search...
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Genome annotation databases
GeneID	5999601.
GenomeReviews	Gene locus gbeA in contig AP007175_GR.
KEGG	aor:AO090010000483.
Enzyme and pathway databases
BRENDA	2.4.1.18. 2240.
Family and domain databases
InterPro	IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLGB_ASPOR

Accession

Primary (citable) accession number: Q96VA4
Secondary accession number(s): Q2TWN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	December 1, 2001
Last modified:	September 22, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)