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Protein

1,4-alpha-glucan-branching enzyme

Gene

gbeA

Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei345 – 3451NucleophileBy similarity
Active sitei400 – 4001Proton donorBy similarity

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-EC
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Glycogen biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan-branching enzyme (EC:2.4.1.18)
Alternative name(s):
Glycogen-branching enzyme
Gene namesi
Name:gbeA
ORF Names:AO090010000483
OrganismiAspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Taxonomic identifieri510516 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000006564 Componenti: Chromosome 8

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: EnsemblFungi
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6896891,4-alpha-glucan-branching enzymePRO_0000188778Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ96VA4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000175159.
KOiK00700.
OMAiECHIGMS.
OrthoDBiEOG7647B5.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

Q96VA4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTSQAVDSS PPDGTGVIQL DPWLEPFRDA LKQRFSFIEG WVKAINETEG
60 70 80 90 100
GLETFSKGYE RFGLNVQSNG DIIYREWAPN AVQAQLVGEF NNWDVTAHPM
110 120 130 140 150
TKNGFGVWEV TVPAVNGAPA IPHDSKIKIS MVIPSGERIY RIPAWIKRVV
160 170 180 190 200
QDLSVSPTYE AVFWNPPTEK QYKFQYSRPK RPESLRIYEA HVGISSPETK
210 220 230 240 250
VATYKEFTSN MLPRIKYLGY NAIQLMAIME HAYYASFGYQ VNNFFAASSR
260 270 280 290 300
YGTPEDLKEL VDKAHSMGLV VLLDVVHSHA SKNVLDGLNM FDGTDHLYFH
310 320 330 340 350
GGGKGRHELW DSRLFNYGHH EVLRFLLSNL RFWMEEYGFD GFRFDGVTSM
360 370 380 390 400
LYTHHGIGTG FSGGYHEYFG SSVDEEGVMY LTLANEMLHN LYPNCITVAE
410 420 430 440 450
DVSGMPALCL PHSLGGVGFD YRLAMAVPDM YIKLLKEKKD DEWDIGNLSF
460 470 480 490 500
TLTNRRHGEK TIAYAESHDQ ALVGDKTLMM WLCDKEMYTH MSVLTEFTPI
510 520 530 540 550
IERGMALHKL IRLVTHGLGG EGYLNFEGNE FGHPEWLDFP RDGNNNSFWY
560 570 580 590 600
ARRQLNLTED HLLRYKFLND FDRAMQLTEE KYGWLHSPQA YVSLKNETDK
610 620 630 640 650
VLVFERAGLL WIFNFHPTNS FTDYRVGVEQ SGTYRIVLDT DDPAFGGLNR
660 670 680
NLKETRFFTT DLSWNGRSNF LQVYIPTRTA LVLALEETL
Length:689
Mass (Da):78,751
Last modified:December 1, 2001 - v1
Checksum:i2B478A5334064432
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB072730 mRNA. Translation: BAB69770.1.
AP007175 Genomic DNA. Translation: BAE66334.1.
RefSeqiXP_001827467.1. XM_001827415.2.

Genome annotation databases

EnsemblFungiiCADAORAT00004542; CADAORAP00004462; CADAORAG00004542.
GeneIDi5999601.
KEGGiaor:AOR_1_796024.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB072730 mRNA. Translation: BAB69770.1.
AP007175 Genomic DNA. Translation: BAE66334.1.
RefSeqiXP_001827467.1. XM_001827415.2.

3D structure databases

ProteinModelPortaliQ96VA4.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADAORAT00004542; CADAORAP00004462; CADAORAG00004542.
GeneIDi5999601.
KEGGiaor:AOR_1_796024.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000175159.
KOiK00700.
OMAiECHIGMS.
OrthoDBiEOG7647B5.

Enzyme and pathway databases

UniPathwayiUPA00164.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural features of the glycogen branching enzyme encoding genes from aspergilli."
    Sasangka P., Matsuno A., Tanaka A., Akasaka Y., Suyama S., Kano S., Miyazaki M., Akao T., Kato M., Kobayashi T., Tsukagoshi N.
    Microbiol. Res. 157:337-344(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ATCC 42149 / RIB 40.
  2. "Genome sequencing and analysis of Aspergillus oryzae."
    Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.
    , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H.
    Nature 438:1157-1161(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 42149 / RIB 40.

Entry informationi

Entry nameiGLGB_ASPOR
AccessioniPrimary (citable) accession number: Q96VA4
Secondary accession number(s): Q2TWN9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: December 1, 2001
Last modified: April 1, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.