Q96UY2 (DGT2A_UMBRA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Diacylglycerol O-acyltransferase 2A
EC=2.3.1.20

Alternative name(s):
Diglyceride acyltransferase 2A
MrDGAT2A

Gene names

Name:

DGAT2A

Organism

Umbelopsis ramanniana (Oleaginous fungus) (Mortierella ramanniana)

Taxonomic identifier

41833 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Fungi incertae sedis › Basal fungal lineages › Mucoromycotina › Mucorales › Mucorales incertae sedis › Umbelopsis

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis.
Catalytic activity	Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol. Ref.1
Pathway	Glycerolipid metabolism; triacylglycerol biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the diacylglycerol acyltransferase family.
Biophysicochemical properties	pH dependence: Optimum pH is 6.0-8.0. Ref.1

Ontologies

Keywords
Biological process	Glycerol metabolism Lipid metabolism Lipid synthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Acyltransferase Transferase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glycerol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW lipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	diacylglycerol O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 355	355	Diacylglycerol O-acyltransferase 2A		PRO_0000249055
Regions
	Transmembrane	41 – 61	21	Helical; Potential
	Transmembrane	62 – 78	17	Helical; Potential
Amino acid modifications
	Glycosylation	142	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q96UY2 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 860316C694058EC0	FASTA	355	40,603
	10 20 30 40 50 60 MASKDQHLQQ KVKHTLEAIP SPRYAPLRVP LRRRLQTLAV LLWCSMMSIC MFIFFFLCSI 70 80 90 100 110 120 PVLLWFPIIL YLTWILVWDK APENGGRPIR WLRNAAWWKL FAGYFPAHVI KEADLDPSKN 130 140 150 160 170 180 YIFGYHPHGI ISMGSFCTFS TNATGFDDLF PGIRPSLLTL TSNFNIPLYR DYLMACGLCS 190 200 210 220 230 240 VSKTSCQNIL TKGGPGRSIA IVVGGASESL NARPGVMDLV LKRRFGFIKI AVQTGASLVP 250 260 270 280 290 300 TISFGENELY EQIESNENSK LHRWQKKIQH ALGFTMPLFH GRGVFNYDFG LLPHRHPIYT 310 320 330 340 350 IVGKPIPVPS IKYGQTKDEI IRELHDSYMH AVQDLYDRYK DIYAKDRVKE LEFVE

References

[1]

"DGAT2 is a new diacylglycerol acyltransferase gene family. purification, cloning, and expression in insect cells of two polypeptides from Mortierella ramanniana with diacylglycerol acyltransferase activity." Lardizabal K.D., Mai J.T., Wagner N.W., Wyrick A., Voelker T., Hawkins D.J. J. Biol. Chem. 276:38862-38869(2001) [PubMed: 11481333] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME ACTIVITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF391089 mRNA. Translation: AAK84179.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR007130. DAGAT. [Graphical view]
PANTHER	PTHR12317. DAGAT. 1 hit.
Pfam	PF03982. DAGAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DGT2A_UMBRA

Accession

Primary (citable) accession number: Q96UY2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	December 1, 2001
Last modified:	May 31, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program