Reviewed, UniProtKB/Swiss-Prot Q96UY1 (DGT2B_UMBRA)

Names and origin

Protein names

Recommended name:
    Diacylglycerol O-acyltransferase 2B
    EC=2.3.1.20
Alternative name(s):
    Diglyceride acyltransferase 2B
    MrDGAT2B

Gene names

Name:

DGAT2B

Organism

Umbelopsis ramanniana (Oleaginous fungus) (Mortierella ramanniana)

Taxonomic identifier

41833 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Fungi incertae sedis › Basal fungal lineages › Mucoromycotina › Mucorales › Mucorales incertae sedis › Umbelopsis

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis.
Catalytic activity	Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol. Ref.1
Pathway	Glycerolipid metabolism; triacylglycerol biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the diacylglycerol acyltransferase family.
Biophysicochemical properties	pH dependence: Optimum pH is 6.0-8.0.

Ontologies

Keywords
Biological process	Glycerol metabolism Lipid metabolism Lipid synthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Acyltransferase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glycerol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW lipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	diacylglycerol O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	Diacylglycerol O-acyltransferase 2B		PRO_0000249056
Regions
	Transmembrane	44 – 64	21	Potential
	Transmembrane	114 – 134	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q96UY1-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 581B61757224015F	FASTA	349	39,596
	10 20 30 40 50 60 MEQVQVTALL DHIPKVHWAP LRGIPLKRRL QTSAIVTWLA LLPICLIIYL YLFTIPLLWP 70 80 90 100 110 120 ILIMYTIWLF FDKAPENGGR RISLVRKLPL WKHFANYFPV TLIKEGDLDP KGNYIMSYHP 130 140 150 160 170 180 HGIISMAAFA NFATEATGFS EQYPGIVPSL LTLASNFRLP LYRDFMMSLG MCSVSRHSCE 190 200 210 220 230 240 AILRSGPGRS IVIVTGGASE SLSARPGTND LTLKKRLGFI RLAIRNGASL VPIFSFGEND 250 260 270 280 290 300 IYEQYDNKKG SLIWRYQKWF QKITGFTVPL AHARGIFNYN AGFIPFRHPI VTVVGKPIAV 310 320 330 340 PLLAEGETEP SEEQMHQVQA QYIESLQAIY DKYKDIYAKD RIKDMTMIA

References

[1]

"DGAT2 is a new diacylglycerol acyltransferase gene family. purification, cloning, and expression in insect cells of two polypeptides from Mortierella ramanniana with diacylglycerol acyltransferase activity." Lardizabal K.D., Mai J.T., Wagner N.W., Wyrick A., Voelker T., Hawkins D.J. J. Biol. Chem. 276:38862-38869(2001) [PubMed: 11481333] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME ACTIVITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF391090 mRNA. Translation: AAK84180.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.3.1.20. 293107.
Family and domain databases
InterPro	IPR007130. DAGAT. [Graphical view]
PANTHER	PTHR12317. DAGAT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DGT2B_UMBRA

Accession

Primary (citable) accession number: Q96UY1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	December 1, 2001
Last modified:	February 9, 2010
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)