Laccase B precursor - Trametes versicolor (White-rot fungus)

Preferred order of sections

Names and origin

Protein names

Submitted name:
Laccase B EMBL AAL07440.1
EC=1.10.3.2 EMBL AAL07440.1

Gene names

Name:

lac1 EMBL AAL07440.1

Organism

Trametes versicolor (White-rot fungus) (Coriolus versicolor) EMBL AAL07440.1

Taxonomic identifier

5325 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Aphyllophorales › Trametes

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

Ontologies

Keywords
Domain	Signal EMBL AAL07440.1
Ligand	Copper SAAS SAAS002355 PDB 1KYA Metal-binding SAAS SAAS001117 PDB 1KYA
Molecular function	Oxidoreductase SAAS SAAS002355 EMBL AAL07440.1
Technical term	3D-structure PDB 1KYA
Gene Ontology (GO)
Molecular function	copper ion binding Inferred from electronic annotation. Source: InterPro hydroquinone:oxygen oxidoreductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 21

21

Potential EMBL AAL07440.1

Chain

22 – 520

499

Potential EMBL AAL07440.1

PRO_5000061322

Regions

Region

75 – 77

3

N-acetyl-D-glucosamine binding PDB 1KYA

Region

354 – 357

4

N-acetyl-D-glucosamine binding PDB 1KYA

Sites

Metal binding

85

1

Copper 1; via tele nitrogen PDB 1KYA

Metal binding

132

1

Copper 1; via tele nitrogen PDB 1KYA

Metal binding

416

1

Copper 2; via pros nitrogen PDB 1KYA

Metal binding

419

1

Copper 1; via tele nitrogen PDB 1KYA

Metal binding

421

1

Copper 1; via tele nitrogen PDB 1KYA

Metal binding

473

1

Copper 1; via tele nitrogen PDB 1KYA

Metal binding

474

1

Copper 2 PDB 1KYA

Metal binding

479

1

Copper 2; via pros nitrogen PDB 1KYA

Binding site

176

1

N-acetyl-D-glucosamine; via amide nitrogen PDB 1KYA

Binding site

238

1

N-acetyl-D-glucosamine PDB 1KYA

Binding site

457

1

N-acetyl-D-glucosamine PDB 1KYA

Sequences

Sequence

Length

Mass (Da)

Tools

Q96UT7 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: F3241CB929C247F9
Show »

FASTA

520

55,576

        10         20         30         40         50         60 
MSRFHSLFAF VVASLTAVAH AGIGPVADLT ITNAAVSPDG FSRQAVVVNG GTPGPLITGN 

        70         80         90        100        110        120 
MGDRFQLNVI DNLTNHTMLK STSIHWHGFF QKGTNWADGP AFINQCPISS GHSFLYDFQV 

       130        140        150        160        170        180 
PDQAGTFWYH SHLSTQYCDG LRGPFVVYDP NDPAADLYDV DNDDTVITLV DWYHVAAKLG 

       190        200        210        220        230        240 
PAFPLGADAT LINGKGRSPS TTTADLSVIS VTPGKRYRFR LVSLSCDPNY TFSIDGHNMT 

       250        260        270        280        290        300 
IIETDSINTA PLVVDSIQIF AAQRYSFVLE ANQAVDNYWI RANPNFGNVG FTGGINSAIL 

       310        320        330        340        350        360 
RYDGAAAVEP TTTQTTSTAP LNEVNLHPLV ATAVPGSPVA GGVDLAINMA FNFNGTNFFI 

       370        380        390        400        410        420 
NGASFTPPTV PVLLQIISGA QNAQDLLPSG SVYSLPSNAD IEISFPATAA APGAPHPFHL 

       430        440        450        460        470        480 
HGHAFAVVRS AGSTVYNYDN PIFRDVVSTG TPAAGDNVTI RFRTDNPGPW FLHCHIDFHL 

       490        500        510        520 
EAGFAVVFAE DIPDVASANP VPQAWSDLCP TYDARDPSDQ

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References

[1]	"Expression of laccase IIIb from the white-rot fungus Trametes versicolor in the yeast Yarrowia lipolytica for environmental applications." Jolivalt C., Madzak C., Brault A., Caminade E., Malosse C., Mougin C. Appl. Microbiol. Biotechnol. 66:450-456(2005) [PubMed: 15349699] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 32745 EMBL AAL07440.1.
[2]	"Crystal structure of a four-copper laccase complexed with an arylamine: insights into substrate recognition and correlation with kinetics." Bertrand T., Jolivalt C., Briozzo P., Caminade E., Joly N., Madzak C., Mougin C. Biochemistry 41:7325-7333(2002) [PubMed: 12044164] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 22-520 IN COMPLEX WITH COPPER AND N-ACETYL-D-GLUCOSAMINE.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF414109 mRNA. Translation: AAL07440.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KYA	X-ray	2.40	A/B/C/D	22-520	[»]

ProteinModelPortal

Q96UT7.

SMR

Q96UT7. Positions 22-520.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
[Graphical view]

Gene3D

G3DSA:2.60.40.420. Cupredoxin. 3 hits.

Pfam

PF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]

SUPFAM

SSF49503. Cupredoxin. 3 hits.

PROSITE

PS00079. MULTICOPPER_OXIDASE1. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q96UT7_TRAVE

Accession

Primary (citable) accession number: Q96UT7

Entry history

Integrated into UniProtKB/TrEMBL:	December 1, 2001
Last sequence update:	December 1, 2001
Last modified:	November 16, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)