Reviewed, UniProtKB/Swiss-Prot Q96UJ9 (DHE4_HEBCY)

Names and origin

Protein names	Recommended name:     NADP-specific glutamate dehydrogenase       Short name=NADP-GDH     EC=1.4.1.4 Alternative name(s):     NADP-dependent glutamate dehydrogenase
Organism	Hebeloma cylindrosporum
Taxonomic identifier	76867 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Agaricomycetidae › Agaricales › Cortinariaceae › Hebeloma

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	L-glutamate + H(2)O + NADP(+) = 2-oxoglutarate + NH(3) + NADPH.
Subunit structure	Homohexamer By similarity.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	NADP-specific glutamate dehydrogenase		PRO_0000182788
Sites
	Active site	111	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q96UJ9-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 3D0E79953F3E0746	FASTA	450	48,356
	10 20 30 40 50 60 MVKPFEPEFQ QALDELTTSL QPFLDANPQY KKALEIVQVP ERVLQFRVVW EDDQGVAQVN 70 80 90 100 110 120 HGFRVQYNSA LGPYKGGLRL HPSVNLSILK FLGFEQTFKN ALTGLSMGGG KGGSDFDPKG 130 140 150 160 170 180 KSDSEIRRFC FSFMGELFRH IGSDTDVPAG DIGTGGREIG FLFGAYKKLR NEFTGMLTGK 190 200 210 220 230 240 GLTWGGSFIR PEATGYGLIY FVEHMIAKAC PEYSLDKPST LVAISGSGNV AQFTALKVIE 250 260 270 280 290 300 LGATVLSLSD SKGSLISDKG YTKEFIRKVA DLKLKGGSLA SLANEEGYTY HAGARPWTLI 310 320 330 340 350 360 PTIHIALPGA TQNEVSAEEA EALLKAGVRI VAEGSNMGCT ADAIDIFESS RKAGPGGVWY 370 380 390 400 410 420 APGKASNCGG VAVSGLEMAQ NSQRLAWTTQ EVDSKLKDIM AECYGICLTA GTKWSGEELT 430 440 450 DEVLPSLLSG ANVAGFIKVA DAMKAQGDWW

References

[1]

"Nucleotide sequence and expression of NADP(+)-GDH and GS from the ectomycorrhizal fungus Hebeloma cylindrosporum." Rodriguez-Pastrana B., Javelle A., Belleville R., Morel M., Botton B., Jacob C., Chalot M., Brun A. Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: h1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY052476 mRNA. Translation: AAL06075.1.
3D structure databases
HSSP	HSSP built from PDB template 1AUP based on UniProtKB P24295.
ModBase	Search...
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DHase. IPR006096. Glu/Leu/Phe/Val_DHase_C. IPR006097. Glu/Leu/Phe/Val_DHase_dimer. IPR014362. Glu_DHase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE4_HEBCY

Accession

Primary (citable) accession number: Q96UJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	December 1, 2001
Last modified:	November 25, 2008
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)