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Q96UH7

- ALF1_PARBA

UniProt

Q96UH7 - ALF1_PARBA

Protein

Fructose-bisphosphate aldolase 1

Gene

FBA1

Organism
Paracoccidioides lutzii (strain ATCC MYA-826 / Pb01) (Paracoccidioides brasiliensis)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 3 (01 Sep 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.By similarity

    Catalytic activityi

    D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

    Cofactori

    Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei63 – 631Glyceraldehyde 3-phosphateBy similarity
    Active sitei110 – 1101Proton donorBy similarity
    Metal bindingi111 – 1111Zinc 1; catalyticBy similarity
    Metal bindingi145 – 1451Zinc 2By similarity
    Metal bindingi175 – 1751Zinc 2By similarity
    Metal bindingi227 – 2271Zinc 1; catalyticBy similarity
    Binding sitei228 – 2281Dihydroxyacetone phosphate; via amide nitrogenBy similarity
    Metal bindingi266 – 2661Zinc 1; catalyticBy similarity

    GO - Molecular functioni

    1. fructose-bisphosphate aldolase activity Source: UniProtKB-EC
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00183.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fructose-bisphosphate aldolase 1 (EC:4.1.2.13)
    Short name:
    FBP aldolase 1
    Short name:
    FBPA 1
    Alternative name(s):
    Fructose-1,6-bisphosphate aldolase 1
    Gene namesi
    Name:FBA1
    ORF Names:PAAG_01995
    OrganismiParacoccidioides lutzii (strain ATCC MYA-826 / Pb01) (Paracoccidioides brasiliensis)
    Taxonomic identifieri502779 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeOnygenalesmitosporic OnygenalesParacoccidioides
    ProteomesiUP000002059: Unassembled WGS sequence

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 360359Fructose-bisphosphate aldolase 1PRO_0000377379Add
    BLAST

    Expressioni

    Inductioni

    Preferentially expressed in yeast cells, the host parasitic phase.2 Publications

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ96UH7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni267 – 2693Dihydroxyacetone phosphate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    KOiK01624.
    OrthoDBiEOG7HTHSN.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR006411. Fruct_bisP_bact.
    IPR000771. Ketose_bisP_aldolase_II.
    [Graphical view]
    PfamiPF01116. F_bP_aldolase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
    TIGRFAMsiTIGR00167. cbbA. 1 hit.
    TIGR01520. FruBisAldo_II_A. 1 hit.
    PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
    PS00806. ALDOLASE_CLASS_II_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q96UH7-1 [UniParc]FASTAAdd to Basket

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    MGVKDILSRK TGVIVGDDVL RLFQHAQEKV FAIPAINVTS SSTVVAALEA    50
    ARDKNSPIIL QVSQGGAAFF AGKGVPNGKQ EASVAGAIAA AHYIRSIAPS 100
    YGIPVVLHTD HCAKKLLPWL DGMLDADECY FKLHNEPLFS SHMIDLSEES 150
    VEWNIETTAK YLKRAAPMKQ WLEMEIGITG GEEDGVNNES VDNNSLYTQP 200
    EDIYTIYKTL SAISPYFSIA AGFGNVHGVY KPGNVRLHPE LLSKHQAYVK 250
    EKTGSSKNKP VYLVFHGGSG STKAEFKEAI SYGVVKVNLD TDLQYAYLSG 300
    VRDFVLKKKD YLMSAVGNPE GEDKPNKKYF DPRVWIREGE KTMSARVQEA 350
    FDDFNTSNQL 360
    Length:360
    Mass (Da):39,632
    Last modified:September 1, 2009 - v3
    Checksum:i67475F644C58E162
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti248 – 2481Y → H in AAL25625. (PubMed:15588996)Curated
    Sequence conflicti248 – 2481Y → H in AAL34519. (PubMed:15588996)Curated
    Sequence conflicti344 – 3441S → C in AAL25625. (PubMed:15588996)Curated
    Sequence conflicti344 – 3441S → C in AAL34519. (PubMed:15588996)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY057387 Genomic DNA. Translation: AAL25625.2.
    AY233454 mRNA. Translation: AAL34519.2.
    DS572814 Genomic DNA. Translation: EEH39806.1.
    RefSeqiXP_002796107.1. XM_002796061.1.

    Genome annotation databases

    GeneIDi9099250.
    KEGGipbl:PAAG_01995.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY057387 Genomic DNA. Translation: AAL25625.2 .
    AY233454 mRNA. Translation: AAL34519.2 .
    DS572814 Genomic DNA. Translation: EEH39806.1 .
    RefSeqi XP_002796107.1. XM_002796061.1.

    3D structure databases

    ProteinModelPortali Q96UH7.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 9099250.
    KEGGi pbl:PAAG_01995.

    Phylogenomic databases

    KOi K01624.
    OrthoDBi EOG7HTHSN.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00183 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR006411. Fruct_bisP_bact.
    IPR000771. Ketose_bisP_aldolase_II.
    [Graphical view ]
    Pfami PF01116. F_bP_aldolase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001359. F_bP_aldolase_II. 1 hit.
    TIGRFAMsi TIGR00167. cbbA. 1 hit.
    TIGR01520. FruBisAldo_II_A. 1 hit.
    PROSITEi PS00602. ALDOLASE_CLASS_II_1. 1 hit.
    PS00806. ALDOLASE_CLASS_II_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Paracoccidioides brasiliensis presents two different cDNAs encoding homologues of the fructose 1,6-biphosphate aldolase: protein isolation, cloning of the cDNAs and genes, structural, phylogenetic, and expression analysis."
      Carneiro L.C., de Faria F.P., Felipe M.S.S., Pereira M., de Almeida Soares C.M.
      Fungal Genet. Biol. 42:51-60(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PROTEIN SEQUENCE OF 328-339 AND 343-359, INDUCTION.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC MYA-826 / Pb01.
    3. "Two-dimensional electrophoresis and characterization of antigens from Paracoccidioides brasiliensis."
      da Fonseca C.A., Jesuino R.S.A., Felipe M.S.S., Cunha D.A., Brito W.A., de Almeida Soares C.M.
      Microbes Infect. 3:535-542(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-37, INDUCTION.

    Entry informationi

    Entry nameiALF1_PARBA
    AccessioniPrimary (citable) accession number: Q96UH7
    Secondary accession number(s): C1GU00, Q8X219
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 16, 2009
    Last sequence update: September 1, 2009
    Last modified: October 1, 2014
    This is version 61 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3