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Protein

Fructose-bisphosphate aldolase 1

Gene

FBA1

Organism
Paracoccidioides lutzii (strain ATCC MYA-826 / Pb01) (Paracoccidioides brasiliensis)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.By similarity

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit. One is catalytic and the other provides a structural contribution.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (PAAG_06526)
  3. ATP-dependent 6-phosphofructokinase (PAAG_01583)
  4. Fructose-bisphosphate aldolase 1 (FBA1), Fructose-bisphosphate aldolase 2 (FBA2)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei63Glyceraldehyde 3-phosphateBy similarity1
Active sitei110Proton donorBy similarity1
Metal bindingi111Zinc 1; catalyticBy similarity1
Metal bindingi145Zinc 2By similarity1
Metal bindingi175Zinc 2By similarity1
Metal bindingi227Zinc 1; catalyticBy similarity1
Binding sitei228Dihydroxyacetone phosphate; via amide nitrogenBy similarity1
Metal bindingi266Zinc 1; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase 1 (EC:4.1.2.13)
Short name:
FBP aldolase 1
Short name:
FBPA 1
Alternative name(s):
Fructose-1,6-bisphosphate aldolase 1
Gene namesi
Name:FBA1
ORF Names:PAAG_01995
OrganismiParacoccidioides lutzii (strain ATCC MYA-826 / Pb01) (Paracoccidioides brasiliensis)
Taxonomic identifieri502779 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeOnygenalesOnygenales incertae sedisParacoccidioides
Proteomesi
  • UP000002059 Componenti: Partially assembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00003773792 – 360Fructose-bisphosphate aldolase 1Add BLAST359

Expressioni

Inductioni

Preferentially expressed in yeast cells, the host parasitic phase.2 Publications

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi502779.XP_002796107.1.

Structurei

3D structure databases

ProteinModelPortaliQ96UH7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni267 – 269Dihydroxyacetone phosphate bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG4153. Eukaryota.
COG0191. LUCA.
KOiK01624.
OrthoDBiEOG092C385G.

Family and domain databases

CDDicd00946. FBP_aldolase_IIA. 1 hit.
Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000771. FBA_II.
IPR006411. Fruct_bisP_bact.
[Graphical view]
PfamiPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsiTIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q96UH7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVKDILSRK TGVIVGDDVL RLFQHAQEKV FAIPAINVTS SSTVVAALEA
60 70 80 90 100
ARDKNSPIIL QVSQGGAAFF AGKGVPNGKQ EASVAGAIAA AHYIRSIAPS
110 120 130 140 150
YGIPVVLHTD HCAKKLLPWL DGMLDADECY FKLHNEPLFS SHMIDLSEES
160 170 180 190 200
VEWNIETTAK YLKRAAPMKQ WLEMEIGITG GEEDGVNNES VDNNSLYTQP
210 220 230 240 250
EDIYTIYKTL SAISPYFSIA AGFGNVHGVY KPGNVRLHPE LLSKHQAYVK
260 270 280 290 300
EKTGSSKNKP VYLVFHGGSG STKAEFKEAI SYGVVKVNLD TDLQYAYLSG
310 320 330 340 350
VRDFVLKKKD YLMSAVGNPE GEDKPNKKYF DPRVWIREGE KTMSARVQEA
360
FDDFNTSNQL
Length:360
Mass (Da):39,632
Last modified:September 1, 2009 - v3
Checksum:i67475F644C58E162
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti248Y → H in AAL25625 (PubMed:15588996).Curated1
Sequence conflicti248Y → H in AAL34519 (PubMed:15588996).Curated1
Sequence conflicti344S → C in AAL25625 (PubMed:15588996).Curated1
Sequence conflicti344S → C in AAL34519 (PubMed:15588996).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY057387 Genomic DNA. Translation: AAL25625.2.
AY233454 mRNA. Translation: AAL34519.2.
KN293995 Genomic DNA. Translation: EEH39806.1.
RefSeqiXP_002796107.1. XM_002796061.2.

Genome annotation databases

EnsemblFungiiEEH39806; EEH39806; PAAG_01995.
GeneIDi9099250.
KEGGipbl:PAAG_01995.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY057387 Genomic DNA. Translation: AAL25625.2.
AY233454 mRNA. Translation: AAL34519.2.
KN293995 Genomic DNA. Translation: EEH39806.1.
RefSeqiXP_002796107.1. XM_002796061.2.

3D structure databases

ProteinModelPortaliQ96UH7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi502779.XP_002796107.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEEH39806; EEH39806; PAAG_01995.
GeneIDi9099250.
KEGGipbl:PAAG_01995.

Phylogenomic databases

eggNOGiKOG4153. Eukaryota.
COG0191. LUCA.
KOiK01624.
OrthoDBiEOG092C385G.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.

Family and domain databases

CDDicd00946. FBP_aldolase_IIA. 1 hit.
Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000771. FBA_II.
IPR006411. Fruct_bisP_bact.
[Graphical view]
PfamiPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsiTIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALF1_PARBA
AccessioniPrimary (citable) accession number: Q96UH7
Secondary accession number(s): C1GU00, Q8X219
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: September 1, 2009
Last modified: November 30, 2016
This is version 73 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.