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Q96UH7

- ALF1_PARBA

UniProt

Q96UH7 - ALF1_PARBA

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Protein

Fructose-bisphosphate aldolase 1

Gene

FBA1

Organism
Paracoccidioides lutzii (strain ATCC MYA-826 / Pb01) (Paracoccidioides brasiliensis)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.By similarity

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Cofactori

Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei63 – 631Glyceraldehyde 3-phosphateBy similarity
Active sitei110 – 1101Proton donorBy similarity
Metal bindingi111 – 1111Zinc 1; catalyticBy similarity
Metal bindingi145 – 1451Zinc 2By similarity
Metal bindingi175 – 1751Zinc 2By similarity
Metal bindingi227 – 2271Zinc 1; catalyticBy similarity
Binding sitei228 – 2281Dihydroxyacetone phosphate; via amide nitrogenBy similarity
Metal bindingi266 – 2661Zinc 1; catalyticBy similarity

GO - Molecular functioni

  1. fructose-bisphosphate aldolase activity Source: UniProtKB-EC
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase 1 (EC:4.1.2.13)
Short name:
FBP aldolase 1
Short name:
FBPA 1
Alternative name(s):
Fructose-1,6-bisphosphate aldolase 1
Gene namesi
Name:FBA1
ORF Names:PAAG_01995
OrganismiParacoccidioides lutzii (strain ATCC MYA-826 / Pb01) (Paracoccidioides brasiliensis)
Taxonomic identifieri502779 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeOnygenalesmitosporic OnygenalesParacoccidioides
ProteomesiUP000002059: Unassembled WGS sequence

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 360359Fructose-bisphosphate aldolase 1PRO_0000377379Add
BLAST

Expressioni

Inductioni

Preferentially expressed in yeast cells, the host parasitic phase.2 Publications

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ96UH7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni267 – 2693Dihydroxyacetone phosphate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

KOiK01624.
OrthoDBiEOG7HTHSN.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006411. Fruct_bisP_bact.
IPR000771. Ketose_bisP_aldolase_II.
[Graphical view]
PfamiPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsiTIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q96UH7 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGVKDILSRK TGVIVGDDVL RLFQHAQEKV FAIPAINVTS SSTVVAALEA
60 70 80 90 100
ARDKNSPIIL QVSQGGAAFF AGKGVPNGKQ EASVAGAIAA AHYIRSIAPS
110 120 130 140 150
YGIPVVLHTD HCAKKLLPWL DGMLDADECY FKLHNEPLFS SHMIDLSEES
160 170 180 190 200
VEWNIETTAK YLKRAAPMKQ WLEMEIGITG GEEDGVNNES VDNNSLYTQP
210 220 230 240 250
EDIYTIYKTL SAISPYFSIA AGFGNVHGVY KPGNVRLHPE LLSKHQAYVK
260 270 280 290 300
EKTGSSKNKP VYLVFHGGSG STKAEFKEAI SYGVVKVNLD TDLQYAYLSG
310 320 330 340 350
VRDFVLKKKD YLMSAVGNPE GEDKPNKKYF DPRVWIREGE KTMSARVQEA
360
FDDFNTSNQL
Length:360
Mass (Da):39,632
Last modified:September 1, 2009 - v3
Checksum:i67475F644C58E162
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti248 – 2481Y → H in AAL25625. (PubMed:15588996)Curated
Sequence conflicti248 – 2481Y → H in AAL34519. (PubMed:15588996)Curated
Sequence conflicti344 – 3441S → C in AAL25625. (PubMed:15588996)Curated
Sequence conflicti344 – 3441S → C in AAL34519. (PubMed:15588996)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY057387 Genomic DNA. Translation: AAL25625.2.
AY233454 mRNA. Translation: AAL34519.2.
DS572814 Genomic DNA. Translation: EEH39806.1.
RefSeqiXP_002796107.1. XM_002796061.1.

Genome annotation databases

GeneIDi9099250.
KEGGipbl:PAAG_01995.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY057387 Genomic DNA. Translation: AAL25625.2 .
AY233454 mRNA. Translation: AAL34519.2 .
DS572814 Genomic DNA. Translation: EEH39806.1 .
RefSeqi XP_002796107.1. XM_002796061.1.

3D structure databases

ProteinModelPortali Q96UH7.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 9099250.
KEGGi pbl:PAAG_01995.

Phylogenomic databases

KOi K01624.
OrthoDBi EOG7HTHSN.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00183 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR006411. Fruct_bisP_bact.
IPR000771. Ketose_bisP_aldolase_II.
[Graphical view ]
Pfami PF01116. F_bP_aldolase. 1 hit.
[Graphical view ]
PIRSFi PIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsi TIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEi PS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Paracoccidioides brasiliensis presents two different cDNAs encoding homologues of the fructose 1,6-biphosphate aldolase: protein isolation, cloning of the cDNAs and genes, structural, phylogenetic, and expression analysis."
    Carneiro L.C., de Faria F.P., Felipe M.S.S., Pereira M., de Almeida Soares C.M.
    Fungal Genet. Biol. 42:51-60(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PROTEIN SEQUENCE OF 328-339 AND 343-359, INDUCTION.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC MYA-826 / Pb01.
  3. "Two-dimensional electrophoresis and characterization of antigens from Paracoccidioides brasiliensis."
    da Fonseca C.A., Jesuino R.S.A., Felipe M.S.S., Cunha D.A., Brito W.A., de Almeida Soares C.M.
    Microbes Infect. 3:535-542(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-37, INDUCTION.

Entry informationi

Entry nameiALF1_PARBA
AccessioniPrimary (citable) accession number: Q96UH7
Secondary accession number(s): C1GU00, Q8X219
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: September 1, 2009
Last modified: October 1, 2014
This is version 61 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3