Reviewed, UniProtKB/Swiss-Prot Q96UF2 (G3P2_RHIRA)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase 2
      Short name=GAPDH 2
    EC=1.2.1.12

Gene names

Name:

GPD2

Organism

Rhizomucor racemosus (Mucor circinelloides f. lusitanicus)

Taxonomic identifier

4841 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Fungi incertae sedis › Basal fungal lineages › Mucoromycotina › Mucorales › Mucoraceae › Mucor

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Glyceraldehyde-3-phosphate dehydrogenase 2		PRO_0000145577
Regions
	Nucleotide binding	12 – 13	2	NAD By similarity
	Region	150 – 152	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	210 – 211	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	151	1	Nucleophile By similarity
	Binding site	34	1	NAD By similarity
	Binding site	79	1	NAD; via carbonyl oxygen By similarity
	Binding site	181	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	233	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	315	1	NAD By similarity
	Site	178	1	Activates thiol group during catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q96UF2-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: DE0D19A20D797AEF	FASTA	338	35,864
	10 20 30 40 50 60 MVTQVGINGF GRIGRIVLRA SLSNPEVQVV AINDPFIPLE YMVYMFKYDS VHGRFQGTVE 70 80 90 100 110 120 AKDGKLVVNG KEISVFSERD PAQIPWGSVE AAYVVESTGV FTSIDAASAH LQGGAKKVII 130 140 150 160 170 180 SAPSGDAPMF VCGVNLEKYT SDLKVISNAS CTTNCLAPLA KVINDNFGIV EGLMTTVHAT 190 200 210 220 230 240 TATQKTVDGP SNKDWRGGRG AGANIIPSST GAAKAVGKVI PELNGKLTGM AFRVPTPDVS 250 260 270 280 290 300 VVDLTVRLEK GATYEEIKAV IKKASENELK GILGYTNDQV VSTDFVGDAQ SSIFDAAAGI 310 320 330 ALNDKFVKLV SWYDNEFGYS NRVIDLLAYA AKVDAAAQ

References

[1]

"Cloning of glyceraldehyde-3-phosphate dehydrogenase-encoding genes in Mucor circinelloides (Syn. racemosus) and use of the gpd1 promoter for recombinant protein production." Wolff A.M., Arnau J. Fungal Genet. Biol. 35:21-29(2002) [PubMed: 11860262] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 90680 / R7B.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ293013 Genomic DNA. Translation: CAC37404.1.
3D structure databases
HSSP	HSSP built from PDB template 1DSS based on UniProtKB P56649.
SMR	Q96UF2. Positions 4-331.
ModBase	Search...
Family and domain databases
InterPro	IPR000173. GlycerAld_3-P_DHase. IPR006424. Glyceraldehyde-3-P_DHase_1. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P2_RHIRA

Accession

Primary (citable) accession number: Q96UF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 9, 2003
Last sequence update:	December 1, 2001
Last modified:	November 25, 2008
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)