Q96UF1 (G3P3_RHIRA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase 3
Short name=GAPDH 3
EC=1.2.1.12

Gene names

Name:

GPD3

Organism

Rhizomucor racemosus (Mucor circinelloides f. lusitanicus)

Taxonomic identifier

4841 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Fungi incertae sedis › Basal fungal lineages › Mucoromycotina › Mucorales › Mucoraceae › Mucor

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Glyceraldehyde-3-phosphate dehydrogenase 3		PRO_0000145578
Regions
	Nucleotide binding	13 – 14	2	NAD By similarity
	Region	151 – 153	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	211 – 212	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	152	1	Nucleophile By similarity
	Binding site	35	1	NAD By similarity
	Binding site	80	1	NAD; via carbonyl oxygen By similarity
	Binding site	182	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	234	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	317	1	NAD By similarity
	Site	179	1	Activates thiol group during catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q96UF1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: E64470AAABA71BC8	FASTA	339	36,302
	10 20 30 40 50 60 MSTINTGING FRSHWPYCLA CSLENPKVKV VAINDPFIDL EYMVYMFKYD STHGRFKGTV 70 80 90 100 110 120 EHKDGKLVVN GHEIAVHAER DPAQIPWGSH GADYVIESTG VFTTKDAASA HLKGGAKKVI 130 140 150 160 170 180 ISAPSADAPM FVVGVNLDKY TSDLTVISNA SCTTNCLAPL AKVIHDNYGI LEGLMTTVHA 190 200 210 220 230 240 TTATQKTVDG PSNKDWRGGR GAGANIIPSS TGAAKAVGKV IPELNGKLTG MAFRVPTPDV 250 260 270 280 290 300 SVVDLTVRLE KPASYDEIKA TIKKASESEE LKGILGYTED QVVSTDFVGD AHSSIFDAKA 310 320 330 GIQLSPTFVK LISWYDNEFG YSTRVVDLLA YVAGKDGAL

References

[1]	"Cloning of glyceraldehyde-3-phosphate dehydrogenase-encoding genes in Mucor circinelloides (Syn. racemosus) and use of the gpd1 promoter for recombinant protein production." Wolff A.M., Arnau J. Fungal Genet. Biol. 35:21-29(2002) [PubMed: 11860262] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 90680 / R7B.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ293014 Genomic DNA. Translation: CAC37405.2.
3D structure databases
ProteinModelPortal	Q96UF1.
SMR	Q96UF1. Positions 4-336.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR020831. GlycerAld/Erythrose_P_DH. IPR020830. GlycerAld_3-P_DH_AS. IPR020829. GlycerAld_3-P_DH_cat. IPR020828. GlycerAld_3-P_DH_NAD(P)-bd. IPR006424. Glyceraldehyde-3-P_DH_1. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
SMART	SM00846. Gp_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P3_RHIRA

Accession

Primary (citable) accession number: Q96UF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 9, 2003
Last sequence update:	December 1, 2001
Last modified:	December 14, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program