Q96UB1 (TMLH_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trimethyllysine dioxygenase
EC=1.14.11.8

Alternative name(s):
Epsilon-trimethyllysine 2-oxoglutarate dioxygenase
TML hydroxylase
TML-alpha-ketoglutarate dioxygenase
Short name=TML dioxygenase
Short name=TMLD

Gene names

Name:	cbs-1
ORF Names:	99H12.050, NCU03802

Organism

Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)

Taxonomic identifier

367110 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Converts trimethyllysine (TML) into hydroxytrimethyllysine (HTML).
Catalytic activity	N6,N6,N(6)-trimethyl-L-lysine + 2-oxoglutarate + O2 = 3-hydroxy-N6,N6,N(6)-trimethyl-L-lysine + succinate + CO2.
Cofactor	Binds 1 Fe2+ ion per subunit By similarity. Ascorbate.
Pathway	Amine and polyamine biosynthesis; carnitine biosynthesis.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the gamma-BBH/TMLD family.
Sequence caution	The sequence CAD11356.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence EAA31955.2 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Carnitine biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carnitine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW trimethyllysine dioxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 471	471	Trimethyllysine dioxygenase		PRO_0000207091
Regions
	Compositional bias	278 – 288	11	Poly-Pro
Sites
	Metal binding	251	1	Iron; catalytic By similarity
	Metal binding	253	1	Iron; catalytic By similarity
	Metal binding	430	1	Iron; catalytic By similarity
Experimental info
	Sequence conflict	174	1	S → P in CAD12887. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q96UB1 [UniParc]. Last modified June 20, 2002. Version 2. Checksum: EC394240DD720F77	FASTA	471	52,630
	10 20 30 40 50 60 MRPQVVGAIL RSRAVVSRQP LSRTHIFAAV TVAKSSSPAQ NSRRTFSSSF RRLYEPKAEI 70 80 90 100 110 120 TAEGLELSPP QAVTGGKRTV LPNFWLRDNC RCTKCVNQDT LQRNFNTFAI PSDIHPTKVE 130 140 150 160 170 180 ATKENVTVQW SDNHTSTYPW PFLSFYLTSN ARGHENDQIS LWGSEAGSRP PTVSFPRVMA 190 200 210 220 230 240 SDQGVADLTA MIKEFGFCFV KDTPHDDPDV TRQLLERIAF IRVTHYGGFY DFTPDLAMAD 250 260 270 280 290 300 TAYTNLALPA HTDTTYFTDP AGLQAFHLLE HKAAPSRPPP PPPPPPPPSE EKEAAGSAAG 310 320 330 340 350 360 EAAAAAEGGK SLLVDGFNAA RILKEEDPRA YEILSSVRLP WHASGNEGIT IAPDKLYPVL 370 380 390 400 410 420 ELNEDTGELH RVRWNNDDRG VVPFGEKYSP SEWYEAARKW DGILRRKSSE LWVQLEPGKP 430 440 450 460 470 LIFDNWRVLH GRSAFSGIRR ICGGYINRDD FISRWRNTNY PRSEVLPRVT G

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Carnitine biosynthesis in Neurospora crassa: identification of a cDNA coding for epsilon-N-trimethyllysine hydroxylase and its functional expression in Saccharomyces cerevisiae." Swiegers J.H., Vaz F.M., Pretorius I.S., Wanders R.J.A., Bauer F.F. FEMS Microbiol. Lett. 210:19-23(2002) [PubMed: 12023072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence." Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U. Nucleic Acids Res. 31:1944-1954(2003) [PubMed: 12655011] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ421151 mRNA. Translation: CAD12887.1. AL451018 Genomic DNA. Translation: CAD11356.1. Sequence problems. AABX02000011 Genomic DNA. Translation: EAA31955.2. Sequence problems.
RefSeq	XP_961191.2. XM_956098.2.
UniGene	Ncr.7824.
3D structure databases
ProteinModelPortal	Q96UB1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	EFNCRT00000003501; EFNCRP00000003501; EFNCRG00000003497.
GeneID	3877351.
KEGG	ncr:NCU03802.
Phylogenomic databases
eggNOG	fuNOG07855.
GeneTree	EFGT00050000002325.
OrthoDB	EOG4DFSXN.
Family and domain databases
InterPro	IPR010376. DUF971. IPR003819. Taurine_dOase. IPR012776. Trimethyllysine_dOase. [Graphical view]
PANTHER	PTHR10696:SF2. tMLys_dOase. 1 hit.
Pfam	PF06155. DUF971. 1 hit. PF02668. TauD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TMLH_NEUCR

Accession

Primary (citable) accession number: Q96UB1
Secondary accession number(s): Q7S7S2, Q8NIQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2002
Last sequence update:	June 20, 2002
Last modified:	October 19, 2011
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program