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Protein

Isocitrate lyase

Gene

ICL1

Organism
Coccidioides immitis (strain RS) (Valley fever fungus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle. Required for growth on ethanol or acetate, but dispensable when fermentable carbon sources are available. Acts also on 2-methylisocitrate.By similarity

Catalytic activityi

Isocitrate = succinate + glyoxylate.By similarity
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate.By similarity

Cofactori

Mg2+By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi169 – 1691MagnesiumBy similarity
Active sitei207 – 2071Proton acceptorBy similarity
Binding sitei244 – 2441SubstrateBy similarity
Binding sitei458 – 4581SubstrateBy similarity

GO - Molecular functioni

  1. isocitrate lyase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. methylisocitrate lyase activity Source: UniProtKB-EC

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-UniPathway
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate lyaseBy similarity (EC:4.1.3.1By similarity)
Short name:
ICLCurated
Short name:
IsocitraseCurated
Short name:
IsocitrataseCurated
Alternative name(s):
Methylisocitrate lyaseBy similarity (EC:4.1.3.30By similarity)
Short name:
MICACurated
Threo-D(S)-isocitrate glyoxylate-lyaseCurated
Gene namesi
Name:ICL1By similarity
ORF Names:CIMG_01988
OrganismiCoccidioides immitis (strain RS) (Valley fever fungus)
Taxonomic identifieri246410 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeOnygenalesmitosporic OnygenalesCoccidioides
ProteomesiUP000001261 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:CIMG_01988.

Subcellular locationi

  1. Glyoxysome By similarity

GO - Cellular componenti

  1. glyoxysome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 538538Isocitrate lyasePRO_0000068788Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ96TP5.
SMRiQ96TP5. Positions 5-521.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni98 – 1003Substrate bindingBy similarity
Regioni208 – 2092Substrate bindingBy similarity
Regioni424 – 4285Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

InParanoidiQ96TP5.
KOiK01637.
OrthoDBiEOG73Z331.

Family and domain databases

Gene3Di3.20.20.60. 2 hits.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERiPTHR21631:SF3. PTHR21631:SF3. 1 hit.
PfamiPF00463. ICL. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q96TP5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLSVEQEEQ KYWEEVEAVK QWWKDSRWRY TKRPFTAEQI VAKRGNLKIE
60 70 80 90 100
YPSNVQSKKL WKLVEEKFKT KTASFTYGCL DPTMVTQMVK YLDTVYVSGW
110 120 130 140 150
QSSSTASSTD EPSPDLADYP MNTVPNKVNQ LWMAQLFHDR KQREERLRNP
160 170 180 190 200
KEKRASLANI DYLAPIIADA DTGHGGLTAV MKLTKLFIER GAAGIHIEDQ
210 220 230 240 250
APGTKKCGHM AGKVLVPISE HINRLVAIRA QADIMGTDLL AIARTDSEAA
260 270 280 290 300
TLITSTIDPR DHAFVVGSTN PNLEPLNDLM IAAERAGKNG AELQAIEDSW
310 320 330 340 350
TAKAGLKRFE DAVIDQIKAS SAANKQAAID AFLREIKGKS NKEARAIARQ
360 370 380 390 400
ILGTDIFWDW DAPRTREGYY RYQGGCQCAI NRAVAFAPFA DLIWMESKLP
410 420 430 440 450
DYAQAKEFAE GVHAVWPQQK LAYNLSPSFN WKAAMPRDEQ ETYIRRLGEL
460 470 480 490 500
GYCWQFITLA GLHTTALISD QFAKAYAKQG MRAYGELVQE PEMENKVDVV
510 520 530
THQKWSGANY VDELLKMVTG GVSSTSAMGK GVTEDQFK
Length:538
Mass (Da):60,383
Last modified:October 3, 2006 - v2
Checksum:iCD722C99DB39502C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti272 – 2721N → T in AAK72548 (Ref. 1) Curated
Sequence conflicti272 – 2721N → T in AAL16915 (Ref. 1) Curated
Sequence conflicti328 – 3292AI → TT in AAK72548 (Ref. 1) Curated
Sequence conflicti328 – 3292AI → TT in AAL16915 (Ref. 1) Curated
Sequence conflicti522 – 5221V → I in AAK72548 (Ref. 1) Curated
Sequence conflicti522 – 5221V → I in AAL16915 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF420484 Genomic DNA. Translation: AAL16915.1.
AY038602 mRNA. Translation: AAK72548.2.
GG704911 Genomic DNA. Translation: EAS36808.2.
RefSeqiXP_001248217.1. XM_001248216.1.
XP_001248391.1. XM_001248390.1.

Genome annotation databases

GeneIDi4567087.
4567700.
KEGGicim:CIMG_01988.
cim:CIMG_02162.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF420484 Genomic DNA. Translation: AAL16915.1.
AY038602 mRNA. Translation: AAK72548.2.
GG704911 Genomic DNA. Translation: EAS36808.2.
RefSeqiXP_001248217.1. XM_001248216.1.
XP_001248391.1. XM_001248390.1.

3D structure databases

ProteinModelPortaliQ96TP5.
SMRiQ96TP5. Positions 5-521.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi4567087.
4567700.
KEGGicim:CIMG_01988.
cim:CIMG_02162.

Organism-specific databases

EuPathDBiFungiDB:CIMG_01988.

Phylogenomic databases

InParanoidiQ96TP5.
KOiK01637.
OrthoDBiEOG73Z331.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.

Family and domain databases

Gene3Di3.20.20.60. 2 hits.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERiPTHR21631:SF3. PTHR21631:SF3. 1 hit.
PfamiPF00463. ICL. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of isocitrate lyase of Coccidioides immitis."
    Shi Q., Ivey F.D., Woitaske M.D., Magee D.M., Cox R.A.
    Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RS.
  3. Cited for: GENOME REANNOTATION.
    Strain: RS.

Entry informationi

Entry nameiACEA_COCIM
AccessioniPrimary (citable) accession number: Q96TP5
Secondary accession number(s): J3KKS8, Q1E625
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 3, 2006
Last modified: April 29, 2015
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.