Q96TP5 (ACEA_COCIM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate lyase
Short name=ICL
Short name=Isocitrase
Short name=Isocitratase
EC=4.1.3.1

Gene names

Name:	ICL1
ORF Names:	CIMG_01988

Organism

Coccidioides immitis (strain RS) (Valley fever fungus) [Reference proteome]

Taxonomic identifier

246410 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Onygenales › mitosporic Onygenales › Coccidioides › Coccidioides immitis

Protein attributes

Sequence length	538 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Isocitrate = succinate + glyoxylate.
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 1/2.
Subunit structure	Homotetramer By similarity.
Subcellular location	Peroxisome By similarity.
Sequence similarities	Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Peroxisome
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	peroxisome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	isocitrate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 538	538	Isocitrate lyase		PRO_0000068788
Sites
	Active site	207	1	By similarity
Experimental info
	Sequence conflict	272	1	N → T in AAK72548. Ref.1
	Sequence conflict	272	1	N → T in AAL16915. Ref.1
	Sequence conflict	328 – 329	2	AI → TT in AAK72548. Ref.1
	Sequence conflict	328 – 329	2	AI → TT in AAL16915. Ref.1
	Sequence conflict	522	1	V → I in AAK72548. Ref.1
	Sequence conflict	522	1	V → I in AAL16915. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q96TP5 [UniParc]. Last modified October 3, 2006. Version 2. Checksum: CD722C99DB39502C	FASTA	538	60,383
	10 20 30 40 50 60 MSLSVEQEEQ KYWEEVEAVK QWWKDSRWRY TKRPFTAEQI VAKRGNLKIE YPSNVQSKKL 70 80 90 100 110 120 WKLVEEKFKT KTASFTYGCL DPTMVTQMVK YLDTVYVSGW QSSSTASSTD EPSPDLADYP 130 140 150 160 170 180 MNTVPNKVNQ LWMAQLFHDR KQREERLRNP KEKRASLANI DYLAPIIADA DTGHGGLTAV 190 200 210 220 230 240 MKLTKLFIER GAAGIHIEDQ APGTKKCGHM AGKVLVPISE HINRLVAIRA QADIMGTDLL 250 260 270 280 290 300 AIARTDSEAA TLITSTIDPR DHAFVVGSTN PNLEPLNDLM IAAERAGKNG AELQAIEDSW 310 320 330 340 350 360 TAKAGLKRFE DAVIDQIKAS SAANKQAAID AFLREIKGKS NKEARAIARQ ILGTDIFWDW 370 380 390 400 410 420 DAPRTREGYY RYQGGCQCAI NRAVAFAPFA DLIWMESKLP DYAQAKEFAE GVHAVWPQQK 430 440 450 460 470 480 LAYNLSPSFN WKAAMPRDEQ ETYIRRLGEL GYCWQFITLA GLHTTALISD QFAKAYAKQG 490 500 510 520 530 MRAYGELVQE PEMENKVDVV THQKWSGANY VDELLKMVTG GVSSTSAMGK GVTEDQFK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of isocitrate lyase of Coccidioides immitis." Shi Q., Ivey F.D., Woitaske M.D., Magee D.M., Cox R.A. Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	"Comparative genomic analyses of the human fungal pathogens Coccidioides and their relatives." Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J., Wortman J.R., Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E., Zeng Q., Hung C.-Y., McMahan C., Muszewska A., Grynberg M., Mandel M.A., Kellner E.M., Barker B.M., Galgiani J.N. , Orbach M.J., Kirkland T.N., Cole G.T., Henn M.R., Birren B.W., Taylor J.W. Genome Res. 19:1722-1731(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RS.
[3]	"Population genomic sequencing of Coccidioides fungi reveals recent hybridization and transposon control." Neafsey D.E., Barker B.M., Sharpton T.J., Stajich J.E., Park D.J., Whiston E., Hung C.-Y., McMahan C., White J., Sykes S., Heiman D., Young S., Zeng Q., Abouelleil A., Aftuck L., Bessette D., Brown A., FitzGerald M. , Lui A., Macdonald J.P., Priest M., Orbach M.J., Galgiani J.N., Kirkland T.N., Cole G.T., Birren B.W., Henn M.R., Taylor J.W., Rounsley S.D. Genome Res. 20:938-946(2010) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: RS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF420484 Genomic DNA. Translation: AAL16915.1. AY038602 mRNA. Translation: AAK72548.2. GG704911 Genomic DNA. Translation: EAS36808.2.
RefSeq	XP_001248217.1. XM_001248216.1. XP_001248391.1. XM_001248390.1.
3D structure databases
ProteinModelPortal	Q96TP5.
SMR	Q96TP5. Positions 5-521.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4567087. 4567700.
KEGG	cim:CIMG_01988. cim:CIMG_02162.
Phylogenomic databases
KO	K01637.
Enzyme and pathway databases
UniPathway	UPA00703; UER00719.
Family and domain databases
Gene3D	3.20.20.60. 2 hits.
InterPro	IPR006254. Isocitrate_lyase. IPR000918. Isocitrate_lyase/Pmutase. IPR018523. Isocitrate_lyase_ph_CS. IPR015813. Pyrv/PenolPyrv_Kinase-like_dom. [Graphical view]
PANTHER	PTHR21631:SF3. PTHR21631:SF3. 1 hit.
Pfam	PF00463. ICL. 1 hit. [Graphical view]
PIRSF	PIRSF001362. Isocit_lyase. 1 hit.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01346. isocit_lyase. 1 hit.
PROSITE	PS00161. ISOCITRATE_LYASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACEA_COCIM

Accession

Primary (citable) accession number: Q96TP5
Secondary accession number(s): J3KKS8, Q1E625

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 3, 2006
Last modified:	May 29, 2013
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program