Reviewed, UniProtKB/Swiss-Prot Q96TL5 (HOG1_NEUCR)

Names and origin

Protein names

Recommended name:
    Mitogen-activated protein kinase hog-1
      Short name=MAP kinase hog-1
    EC=2.7.11.24

Gene names

Name:	hog-1
Synonyms:	os-2
ORF Names:	NCU07024

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Mitogen-activated protein kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Controls osmotic regulation of transcription of target genes. Involved in ion flux-mediated turgor regulation. Ref.2 Ref.5
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by tyrosine and threonine phosphorylation By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modification	Dually phosphorylated on Thr-171 and Tyr-173, which activates the enzyme By similarity. Phosphorylation is induced by fungicides and osmotic stress.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily. HOG1 sub-subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Activator Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	Mitogen-activated protein kinase hog-1		PRO_0000289698
Regions
	Domain	20 – 299	280	Protein kinase
	Nucleotide binding	26 – 34	9	ATP By similarity
	Motif	171 – 173	3	TXY
Sites
	Active site	141	1	Proton acceptor By similarity
	Binding site	49	1	ATP By similarity
Amino acid modifications
	Modified residue	171	1	Phosphothreonine By similarity
	Modified residue	173	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q96TL5-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 7D1A60613CAD37E8	FASTA	358	41,243
	10 20 30 40 50 60 MAEFIRAQIF GTTFEITSRY SDLQPVGMGA FGLVCSAKDQ LTNQNVAIKK IMKPFSTPVL 70 80 90 100 110 120 AKRTYRELKL LKHLRHENVI SLSDIFISPL EDIYFVTELL GTDLHRLLTS RPLEKQFIQY 130 140 150 160 170 180 FLYQIMRGLK YVHSAGVVHR DLKPSNILVN ENCDLKICDF GLARIQDPQM TGYVSTRYYR 190 200 210 220 230 240 APEIMLTWQK YDVEVDIWSA GCIFAEMLEG KPLFPGKDHV NQFSIITELL GTPPDDVINT 250 260 270 280 290 300 IASENTLRFV KSLPKRERQP LKNKFKNADS SAVDLLERML VFDPKKRITA TEALSHEYLA 310 320 330 340 350 PYHDPTDEPV AEEKFDWSFN DADLPVDTWK IMMYSEILDY HNVEASGQMM FQEDVPPQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The hyper-osmotic stress response pathway of Neurospora crassa is the target of phenylpyrrole fungicides." Zhang Y., Lamm R., Pillonel C., Xu J.-R., Lam S. Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Osmoregulation and fungicide resistance: the Neurospora crassa os-2 gene encodes a HOG1 mitogen-activated protein kinase homologue." Zhang Y., Lamm R., Pillonel C., Lam S., Xu J.-R. Appl. Environ. Microbiol. 68:532-538(2002) [PubMed: 11823187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[3]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[4]	"Group III histidine kinase is a positive regulator of Hog1-type mitogen-activated protein kinase in filamentous fungi." Yoshimi A., Kojima K., Takano Y., Tanaka C. Eukaryot. Cell 4:1820-1828(2005) [PubMed: 16278449] [Abstract] Cited for: PHOSPHORYLATION.
[5]	"Role of a mitogen-activated protein kinase cascade in ion flux-mediated turgor regulation in fungi." Lew R.R., Levina N.N., Shabala L., Anderca M.I., Shabala S.N. Eukaryot. Cell 5:480-487(2006) [PubMed: 16524903] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF297031 mRNA. Translation: AAK83124.1. AF297032 Genomic DNA. Translation: AAK83125.1. AABX02000035 Genomic DNA. Translation: EAA32927.2.
RefSeq	XP_962163.2.
3D structure databases
HSSP	HSSP built from PDB template 1KV1 based on UniProtKB Q16539.
ModBase	Search...
Protein-protein interaction databases
STRING	Q96TL5.
Genome annotation databases
GeneID	3878327.
KEGG	ncr:NCU07024.
UCSC	W06B11.4. c. elegans.
Enzyme and pathway databases
BRENDA	2.7.11.24. 266.
Family and domain databases
InterPro	IPR003527. MAP_kinase_CS. IPR008352. MAPK_p38. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PRINTS	PR01773. P38MAPKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HOG1_NEUCR

Accession

Primary (citable) accession number: Q96TL5
Secondary accession number(s): Q7RVG0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	December 1, 2001
Last modified:	September 22, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)