Reviewed, UniProtKB/Swiss-Prot Q96TC7 (RMD3_HUMAN)

Names and origin

Protein names

Recommended name:
    Regulator of microtubule dynamics protein 3
      Short name=RMD-3
      Short name=hRMD-3
Alternative name(s):
    Protein FAM82A2
    Protein FAM82C
    Protein tyrosine phosphatase-interacting protein 51
    TCPTP-interacting protein 51
    Cerebral protein 10

Gene names

Name:	FAM82A2
Synonyms:	FAM82C, PTPIP51
ORF Names:	hucep-10, UNQ3122/PRO10274

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	470 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May participate in differentiation and apoptosis of keratinocytes. Overexpression induces apoptosis. Ref.8
Subunit structure	Interacts with PTPN2. Interacts with microtubules. Ref.6 Ref.7
Subcellular location	Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. Nucleus. Note: In interphase localizes in the cytoplasm, and during mitosis localizes to the spindle microtubules and spindle poles. Ref.8 Ref.6 Ref.9
Tissue specificity	Present at high level in epidermis and seminiferous epithelium: while basal cells in the epidermis and spermatogonia show no perceptible amount, keratinocytes of suprabasal layers and differentiating first-order spermatocytes up to spermatids exhibit high expression. In skeletal muscle, its presence is restricted to fibers of the fast twitch type. In surface epithelia containing ciliated cells, it is associated with the microtubular structures responsible for ciliary movement. Also present in specific structures of the central nervous system such as neurons of the hippocampal region, ganglion cells of the autonomic nervous system, and axons of the peripheral nervous system (at protein level). Widely expressed. Ref.8 Ref.7
Induction	By EGF, TGF-beta, retinoic acid-and 1,25-Dihydroxyvitamin D3. Ref.9
Domain	The transmembrane region is required for mitochondrial localization.
Sequence similarities	Belongs to the FAM82/RMD family.
Sequence caution	The sequence BAB15298.1 differs from that shown. Reason: Erroneous termination at position 453. Translated as Glu. The sequence BAC85554.1 differs from that shown. Reason: Frameshift at position 383.

Ontologies

Keywords
Biological process	Apoptosis Differentiation
Cellular component	Cytoplasm Membrane Microtubule Mitochondrion Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Transmembrane
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	apoptosis Inferred from electronic annotation. Source: UniProtKB-KW cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microtubule Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
YWHAB	P31946	1	EBI-1056589,EBI-359815
YWHAG	P61981	1	EBI-1056589,EBI-359832

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96TC7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96TC7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-129: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 470	470	Regulator of microtubule dynamics protein 3		PRO_0000287510
Regions
	Transmembrane	13 – 35	23	Potential
	Coiled coil	92 – 124	33	Potential
Amino acid modifications
	Modified residue	44	1	Phosphoserine Ref.12
	Modified residue	46	1	Phosphoserine Ref.10 Ref.11
	Modified residue	189	1	Phosphoserine By similarity
	Modified residue	212	1	Phosphoserine Ref.12
Natural variations
	Alternative sequence	1 – 129	129	Missing in isoform 2.		VSP_025531
	Natural variant	33	1	Q → H: dbSNP rs11558807.		VAR_049029
Experimental info
	Sequence conflict	416	1	F → V in BAB46923. Ref.1
	Sequence conflict	469	1	R → Q in BAC85554. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 15, 2007. Version 2. Checksum: 1C8D1022E6CF7B6A	FASTA	470	52,118
	10 20 30 40 50 60 MSRLGALGGA RAGLGLLLGT AAGLGFLCLL YSQRWKRTQR HGRSQSLPNS LDYTQTSDPG 70 80 90 100 110 120 RHVMLLRAVP GGAGDASVLP SLPREGQEKV LDRLDFVLTS LVALRREVEE LRSSLRGLAG 130 140 150 160 170 180 EIVGEVRCHM EENQRVARRR RFPFVRERSD STGSSSVYFT ASSGATFTDA ESEGGYTTAN 190 200 210 220 230 240 AESDNERDSD KESEDGEDEV SCETVKMGRK DSLDLEEEAA SGASSALEAG GSSGLEDVLP 250 260 270 280 290 300 LLQQADELHR GDEQGKREGF QLLLNNKLVY GSRQDFLWRL ARAYSDMCEL TEEVSEKKSY 310 320 330 340 350 360 ALDGKEEAEA ALEKGDESAD CHLWYAVLCG QLAEHESIQR RIQSGFSFKE HVDKAIALQP 370 380 390 400 410 420 ENPMAHFLLG RWCYQVSHLS WLEKKTATAL LESPLSATVE DALQSFLKAE ELQPGFSKAG 430 440 450 460 470 RVYISKCYRE LGKNSEARWW MKLALELPDV TKEDLAIQKD LEELEVILRD
	Isoform 2. Checksum: 6015D71ECA057A27	FASTA	341	38,247
	10 20 30 40 50 60 MEENQRVARR RRFPFVRERS DSTGSSSVYF TASSGATFTD AESEGGYTTA NAESDNERDS 70 80 90 100 110 120 DKESEDGEDE VSCETVKMGR KDSLDLEEEA ASGASSALEA GGSSGLEDVL PLLQQADELH 130 140 150 160 170 180 RGDEQGKREG FQLLLNNKLV YGSRQDFLWR LARAYSDMCE LTEEVSEKKS YALDGKEEAE 190 200 210 220 230 240 AALEKGDESA DCHLWYAVLC GQLAEHESIQ RRIQSGFSFK EHVDKAIALQ PENPMAHFLL 250 260 270 280 290 300 GRWCYQVSHL SWLEKKTATA LLESPLSATV EDALQSFLKA EELQPGFSKA GRVYISKCYR 310 320 330 340 ELGKNSEARW WMKLALELPD VTKEDLAIQK DLEELEVILR D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a novel gene expressed in human cerebral cortex." Yoshimoto M., Yazaki M., Matsumoto K., Takayama K. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Teratocarcinoma.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain and Uterus.
[5]	"A protein interacting with T-cell tyrosine phosphatase." Porsche A., Hofer W.H. Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 78-470.
[6]	"RMD-1, a novel microtubule-associated protein, functions in chromosome segregation in Caenorhabditis elegans." Oishi K., Okano H., Sawa H. J. Cell Biol. 179:1149-1162(2007) [PubMed: 18070910] [Abstract] Cited for: IDENTIFICATION, INTERACTION WITH MICROTUBULES, SUBCELLULAR LOCATION.
[7]	"The novel protein PTPIP51 exhibits tissue- and cell-specific expression." Stenzinger A., Kajosch T., Tag C., Porsche A., Welte I., Hofer H.W., Steger K., Wimmer M. Histochem. Cell Biol. 123:19-28(2005) [PubMed: 15609043] [Abstract] Cited for: TISSUE SPECIFICITY, INTERACTION WITH PTPN2.
[8]	"Protein tyrosine phosphatase interacting protein 51 (PTPIP51) is a novel mitochondria protein with an N-terminal mitochondrial targeting sequence and induces apoptosis." Lv B.F., Yu C.F., Chen Y.Y., Lu Y., Guo J.H., Song Q.S., Ma D.L., Shi T.P., Wang L. Apoptosis 11:1489-1501(2006) [PubMed: 16820967] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[9]	"Epidermal growth factor-, transforming growth factor-beta-, retinoic acid-and 1,25-dihydroxyvitamin D(3)-regulated expression of the novel protein PTPIP51 in keratinocytes." Stenzinger A., Schreiner D., Pfeiffer T., Tag C., Hofer H.W., Wimmer M. Cells Tissues Organs 184:76-87(2006) [PubMed: 17361080] [Abstract] Cited for: INDUCTION, SUBCELLULAR LOCATION.
[10]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-46, MASS SPECTROMETRY.
[11]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-46, MASS SPECTROMETRY.
[12]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-44 AND SER-212, MASS SPECTROMETRY.
[13]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB000782 mRNA. Translation: BAB46923.1. AY358793 mRNA. Translation: AAQ89153.1. AK001441 mRNA. Translation: BAA91693.1. AK025963 mRNA. Translation: BAB15298.1. Sequence problems. AK123192 mRNA. Translation: BAC85554.1. Frameshift. BC008970 mRNA. Translation: AAH08970.2. BC063844 mRNA. Translation: AAH63844.1. AJ242719 Genomic DNA. Translation: CAC39480.1. BR000691 mRNA. Translation: FAA00416.1.
IPI	IPI00410079. IPI00845345.
RefSeq	NP_060615.1.
UniGene	Hs.511067
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96TC7. 2 interactions.
PTM databases
PhosphoSite	Q96TC7.
Proteomic databases
PeptideAtlas	Q96TC7.
PRIDE	Q96TC7.
Genome annotation databases
Ensembl	ENSG00000137824. Homo sapiens. [Contig view]
GeneID	55177.
KEGG	hsa:55177.
UCSC	uc001zmo.1. human.
Organism-specific databases
GeneCards	GC15M038817.
HGNC	HGNC:25550. FAM82A2.
HPA	HPA009975.
MIM	611873. gene.
PharmGKB	PA142671850.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q96TC7.
HOVERGEN	Q96TC7.
OMA	Q96TC7. EVRSHME.
Gene expression databases
ArrayExpress	Q96TC7.
Bgee	Q96TC7.
CleanEx	HS_FAM82A2.
Family and domain databases
InterPro	IPR011990. TPR-like_helical. [Graphical view]
Gene3D	G3DSA:1.25.40.10. TPR-like_helical. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	58979.
SOURCE	Search...

Entry information

Entry name

RMD3_HUMAN

Accession

Primary (citable) accession number: Q96TC7
Secondary accession number(s): A9UMZ9 , Q6ZWE9, Q96H23, Q96SD6, Q9H6G1, Q9NVQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	May 15, 2007
Last modified:	July 7, 2009
This is version 45 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)