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Q96TA1 (NIBL1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Niban-like protein 1
Alternative name(s):
Meg-3
Melanoma invasion by ERK
Short name=MINERVA
Protein FAM129B
Gene names
Name:FAM129B
Synonyms:C9orf88
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length746 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May play a role in apoptosis suppression. May promote melanoma cell invasion in vitro. Ref.8 Ref.15

Subcellular location

Cytoplasmcytosol. Cell junctionadherens junction. Note: In exponentially growing cells, exclusively cytoplasmic. Cell membrane localization is observed when cells reach confluency and during telophase. In melanoma cells, targeting to the plasma membrane may be impaired by C-terminal phosphorylation. Ref.8 Ref.15

Post-translational modification

Phosphorylated at Ser-641, Ser-646, Ser-692 and Ser-696 by the BRAF/MKK/ERK signaling cascade. In melanoma cells, the C-terminal phosphorylation may prevent targeting to the plasma membrane. Ref.8

As apoptosis proceeds, degraded via an proteasome-independent pathway, probably by caspases (Ref.15).

Sequence similarities

Belongs to the Niban family.

Contains 1 PH domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96TA1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96TA1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: MGDVLSTHLDDARRQHIA → MGWMG

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 746746Niban-like protein 1
PRO_0000213121

Regions

Domain68 – 192125PH

Amino acid modifications

Modified residue5681Phosphoserine Ref.13
Modified residue5741Phosphoserine Ref.10
Modified residue6011Phosphoserine Ref.10
Modified residue6031Phosphoserine Ref.10
Modified residue6061Phosphothreonine Ref.10
Modified residue6091Phosphoserine Ref.10
Modified residue6241Phosphoserine Ref.12
Modified residue6381Phosphoserine Ref.10 Ref.13
Modified residue6411Phosphoserine Ref.8 Ref.10 Ref.12 Ref.13
Modified residue6461Phosphoserine Ref.8 Ref.9 Ref.10 Ref.12 Ref.13
Modified residue6651Phosphoserine Ref.8 Ref.9 Ref.12 Ref.13 Ref.16
Modified residue6811Phosphoserine Ref.8 Ref.9 Ref.12 Ref.13 Ref.16
Modified residue6921Phosphoserine Ref.8 Ref.10 Ref.12
Modified residue6961Phosphoserine Ref.8 Ref.10 Ref.12

Natural variations

Alternative sequence1 – 1818MGDVL…RQHIA → MGWMG in isoform 2.
VSP_041810

Experimental info

Mutagenesis6411S → A: Loss of melanoma cell invasion; when associated with A-646; A-665; A-681; A-679 and A-683. Ref.8
Mutagenesis6411S → D or E: Promotes melanoma cell invasion; when associated with D/E-633; D/E-652; D/E-668; D/E-679 and D/E-683. Ref.8
Mutagenesis6461S → A: Loss of melanoma cell invasion; when associated with A-641; A-665; A-681; A-679 and A-683. Ref.8
Mutagenesis6461S → D or E: Promotes melanoma cell invasion; when associated with D/E-628; D/E-652; D/E-668; D/E-679 and D/E-683. Ref.8
Mutagenesis6651S → A: Loss of melanoma cell invasion; when associated with A-641; A-646; A-681; A-679 and A-683. Ref.8
Mutagenesis6651S → D or E: Promotes melanoma cell invasion; when associated with D/E-628; D/E-633; D/E-668; D/E-679 and D/E-683. Ref.8
Mutagenesis6811S → A: Loss of melanoma cell invasion; when associated with A-641; A-646; A-665; A-679 and A-683. Ref.8
Mutagenesis6811S → D or E: Promotes melanoma cell invasion; when associated with D/E-628; D/E-633; D/E-652; D/E-679 and D/E-683. Ref.8
Mutagenesis6921S → A: Loss of melanoma cell invasion; when associated with A-641; A-646; A-665; A-668 and A-683. Ref.8
Mutagenesis6921S → D or E: Promotes melanoma cell invasion; when associated with D/E-628; D/E-633; D/E-652; D/E-668 and D/E-683. Ref.8
Mutagenesis6961S → A: Loss of melanoma cell invasion; when associated with A-641; A-646; A-665; A-668 and A-679. Ref.8
Mutagenesis6961S → D or E: Promotes melanoma cell invasion; when associated with D/E-628; D/E-633; D/E-652; D/E-668 and D/E-679. Ref.8
Sequence conflict3181H → L in AAK57556. Ref.1
Sequence conflict6771P → L in AAH01979. Ref.7

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified September 21, 2011. Version 3.
Checksum: 2049835F08356944

FASTA74684,138
        10         20         30         40         50         60 
MGDVLSTHLD DARRQHIAEK TGKILTEFLQ FYEDQYGVAL FNSMRHEIEG TGLPQAQLLW 

        70         80         90        100        110        120 
RKVPLDERIV FSGNLFQHQE DSKKWRNRFS LVPHNYGLVL YENKAAYERQ VPPRAVINSA 

       130        140        150        160        170        180 
GYKILTSVDQ YLELIGNSLP GTTAKSGSAP ILKCPTQFPL ILWHPYARHY YFCMMTEAEQ 

       190        200        210        220        230        240 
DKWQAVLQDC IRHCNNGIPE DSKVEGPAFT DAIRMYRQSK ELYGTWEMLC GNEVQILSNL 

       250        260        270        280        290        300 
VMEELGPELK AELGPRLKGK PQERQRQWIQ ISDAVYHMVY EQAKARFEEV LSKVQQVQPA 

       310        320        330        340        350        360 
MQAVIRTDMD QIITSKEHLA SKIRAFILPK AEVCVRNHVQ PYIPSILEAL MVPTSQGFTE 

       370        380        390        400        410        420 
VRDVFFKEVT DMNLNVINEG GIDKLGEYME KLSRLAYHPL KMQSCYEKME SLRLDGLQQR 

       430        440        450        460        470        480 
FDVSSTSVFK QRAQIHMREQ MDNAVYTFET LLHQELGKGP TKEELCKSIQ RVLERVLKKY 

       490        500        510        520        530        540 
DYDSSSVRKR FFREALLQIS IPFLLKKLAP TCKSELPRFQ ELIFEDFARF ILVENTYEEV 

       550        560        570        580        590        600 
VLQTVMKDIL QAVKEAAVQR KHNLYRDSMV MHNSDPNLHL LAEGAPIDWG EEYSNSGGGG 

       610        620        630        640        650        660 
SPSPSTPESA TLSEKRRRAK QVVSVVQDEE VGLPFEASPE SPPPASPDGV TEIRGLLAQG 

       670        680        690        700        710        720 
LRPESPPPAG PLLNGAPAGE SPQPKAAPEA SSPPASPLQH LLPGKAVDLG PPKPSDQETG 

       730        740 
EQVSSPSSHP ALHTTTEDSA GVQTEF 

« Hide

Isoform 2 [UniParc].

Checksum: 38E1C24CF737F3DB
Show »

FASTA73382,683

References

« Hide 'large scale' references
[1]"Homo sapiens meg-3 mRNA, complete cds."
Miyata T., Inagi R., Yasuda Y., Kurokawa K.
Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]"Differential screening of human osteoclast maturation associated genes."
Yamane S., Toyosaki-Maeda T., Tsuruta Y., Suzuki R., Ochi T.
Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"DNA sequence and analysis of human chromosome 9."
Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. expand/collapse author list , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"Preparation of a set of expression-ready clones of mammalian long cDNAs encoding large proteins by the ORF trap cloning method."
Nakajima D., Saito K., Yamakawa H., Kikuno R.F., Nakayama M., Ohara R., Okazaki N., Koga H., Nagase T., Ohara O.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 8-746 (ISOFORM 1).
[6]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 38-733.
Tissue: Testis.
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 278-733.
Tissue: Cervix.
[8]"Functional proteomics identifies targets of phosphorylation by B-Raf signaling in melanoma."
Old W.M., Shabb J.B., Houel S., Wang H., Couts K.L., Yen C.Y., Litman E.S., Croy C.H., Meyer-Arendt K., Miranda J.G., Brown R.A., Witze E.S., Schweppe R.E., Resing K.A., Ahn N.G.
Mol. Cell 34:115-131(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 637-650 AND 688-698, IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION AT SER-641; SER-646; SER-665; SER-681; SER-692 AND SER-696, MUTAGENESIS OF SER-641; SER-646; SER-665; SER-681; SER-692 AND SER-696.
[9]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-646; SER-665 AND SER-681, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-574; SER-601; SER-603; THR-606; SER-609; SER-638; SER-641; SER-646; SER-692 AND SER-696, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[11]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-624; SER-641; SER-646; SER-665; SER-681; SER-692 AND SER-696, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[13]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-568; SER-638; SER-641; SER-646; SER-665 AND SER-681, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[14]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[15]"FAM129B/MINERVA, a novel adherens junction-associated protein, suppresses apoptosis in HeLa cells."
Chen S., Evans H.G., Evans D.R.
J. Biol. Chem. 286:10201-10209(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[16]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-665 AND SER-681, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF151783 mRNA. Translation: AAK57556.1.
AF192911 mRNA. Translation: AAQ13825.1.
AL445222, AL390116 Genomic DNA. Translation: CAH72914.1.
AL445222, AL390116 Genomic DNA. Translation: CAH72915.1.
AL390116, AL445222 Genomic DNA. Translation: CAH73084.1.
AL390116, AL445222 Genomic DNA. Translation: CAH73085.1.
CH471090 Genomic DNA. Translation: EAW87675.1.
CH471090 Genomic DNA. Translation: EAW87677.1.
AB210016 mRNA. Translation: BAE06098.1.
AL137555 mRNA. Translation: CAB70809.1.
BC001979 mRNA. Translation: AAH01979.2.
PIRT46394.
RefSeqNP_001030611.1. NM_001035534.2.
NP_073744.2. NM_022833.3.
UniGeneHs.522401.

3D structure databases

ProteinModelPortalQ96TA1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid122328. 25 interactions.
IntActQ96TA1. 5 interactions.
MINTMINT-1193681.
STRING9606.ENSP00000362409.

PTM databases

PhosphoSiteQ96TA1.

Polymorphism databases

DMDM347595791.

Proteomic databases

PaxDbQ96TA1.
PRIDEQ96TA1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000373312; ENSP00000362409; ENSG00000136830. [Q96TA1-1]
ENST00000373314; ENSP00000362411; ENSG00000136830. [Q96TA1-2]
GeneID64855.
KEGGhsa:64855.
UCSCuc004brh.3. human. [Q96TA1-1]
uc004bri.3. human. [Q96TA1-2]

Organism-specific databases

CTD64855.
GeneCardsGC09M130267.
H-InvDBHIX0008401.
HGNCHGNC:25282. FAM129B.
HPAHPA021284.
HPA021417.
HPA023261.
HPA024312.
MIM614045. gene.
neXtProtNX_Q96TA1.
PharmGKBPA162385984.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG46977.
HOGENOMHOG000220836.
HOVERGENHBG026217.
OMARFYEDQY.
OrthoDBEOG7GN2MC.
TreeFamTF333351.

Gene expression databases

ArrayExpressQ96TA1.
BgeeQ96TA1.
CleanExHS_FAM129B.
GenevestigatorQ96TA1.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
InterProIPR026088. Niban_like.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
[Graphical view]
PANTHERPTHR14392. PTHR14392. 1 hit.
SMARTSM00233. PH. 1 hit.
[Graphical view]
PROSITEPS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSFAM129B. human.
GenomeRNAi64855.
NextBio66991.
PROQ96TA1.
SOURCESearch...

Entry information

Entry nameNIBL1_HUMAN
AccessionPrimary (citable) accession number: Q96TA1
Secondary accession number(s): Q4LE55 expand/collapse secondary AC list , Q5VVW6, Q5VVW7, Q9BUS2, Q9NT35
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: September 21, 2011
Last modified: April 16, 2014
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM