Q96T55 (KCNKG_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Potassium channel subfamily K member 16

Alternative name(s):
2P domain potassium channel Talk-1
TWIK-related alkaline pH-activated K(+) channel 1
Short name=TALK-1

Gene names

Name:	KCNK16
Synonyms:	TALK1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Outward rectifying potassium channel. Produces rapidly activating and non-inactivating outward rectifier K+ currents.
Subunit structure	Homodimer Potential.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Highly expressed in pancreas. Not detectable in the other tissues tested. Ref.2
Miscellaneous	Inhibited by Ba2+, quinine, quinidine, chloroform and halothane. Activated at alkaline pH.
Sequence similarities	Belongs to the two pore domain potassium channel (TC 1.A.1.8) family. [View classification]

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
Molecular function	Ion channel Potassium channel Voltage-gated channel
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	synaptic transmission Traceable author statement. Source: Reactome
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Traceable author statement. Source: Reactome
Molecular_function	potassium channel activity Inferred from direct assay Ref.1Ref.2. Source: MGI voltage-gated ion channel activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: Q96T55-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform b (identifier: Q96T55-3)

The sequence of this isoform differs from the canonical sequence as follows:
     269-309: LRQGCGAKAAPGRRPRRGSTAARGVQVTPQDFPISKKGLGS → RGLGVKDGAASDPSGLPRPQKIPISA

Isoform c (identifier: Q96T55-4)

The sequence of this isoform differs from the canonical sequence as follows:
     222-309: TDPSKHYISV...FPISKKGLGS → HPLNFITPSG...PMWLGSSAQV

Isoform d (identifier: Q96T55-5)

The sequence of this isoform differs from the canonical sequence as follows:
     222-268: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Potassium channel subfamily K member 16		PRO_0000101767
Regions
	Topological domain	1 – 13	13	Cytoplasmic Potential
	Transmembrane	14 – 34	21	Helical; Potential
	Intramembrane	98 – 116	19	Pore-forming; Name=Pore-forming 1; Potential
	Transmembrane	120 – 140	21	Helical; Potential
	Topological domain	141 – 165	25	Cytoplasmic Potential
	Transmembrane	166 – 186	21	Helical; Potential
	Intramembrane	202 – 221	20	Pore-forming; Name=Pore-forming 2; Potential
	Transmembrane	238 – 258	21	Helical; Potential
	Topological domain	259 – 309	51	Cytoplasmic Potential
Natural variations
	Alternative sequence	222 – 309	88	TDPSK…KGLGS → HPLNFITPSGLLPSQEPFQT PHGKPESQQIPGSFQKVSSM NVWPLSGMHSPGLAFPLPDC NIPDQERFRPLHPGAWKFWP LPLPSSNSKWAPMWLGSSAQ V in isoform c.		VSP_039863
	Alternative sequence	222 – 268	47	Missing in isoform d.		VSP_039864
	Alternative sequence	269 – 309	41	LRQGC…KGLGS → RGLGVKDGAASDPSGLPRPQ KIPISA in isoform b.		VSP_039865
	Natural variant	215	1	F → L. Corresponds to variant rs9462527 [ dbSNP | Ensembl ].		VAR_063636
	Natural variant	275	1	A → G. Corresponds to variant rs1535500 [ dbSNP | Ensembl ].		VAR_063637
	Natural variant	301	1	P → H. Ref.2 Ref.5 Corresponds to variant rs11756091 [ dbSNP | Ensembl ].		VAR_052430
Experimental info
	Sequence conflict	49	1	L → S in AAP82866. Ref.2
	Sequence conflict	149	1	A → V in AAP82867. Ref.2
	Sequence conflict	200	1	S → G in AAP82866. Ref.2
	Sequence conflict	205 – 207	3	FAF → LLS in AAP82867. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 99C4B11EB26B0764	FASTA	309	34,153
	10 20 30 40 50 60 MPSAGLCSCW GGRVLPLLLA YVCYLLLGAT IFQLLERQAE AQSRDQFQLE KLRFLENYTC 70 80 90 100 110 120 LDQWAMEQFV QVIMEAWVKG VNPKGNSTNP SNWDFGSSFF FAGTVVTTIG YGNLAPSTEA 130 140 150 160 170 180 GQVFCVFYAL LGIPLNVIFL NHLGTGLRAH LAAIERWEDR PRRSQVLQVL GLALFLTLGT 190 200 210 220 230 240 LVILIFPPMV FSHVEGWSFS EGFYFAFITL STIGFGDYVV GTDPSKHYIS VYRSLAAIWI 250 260 270 280 290 300 LLGLAWLALI LPLGPLLLHR CCQLWLLSLR QGCGAKAAPG RRPRRGSTAA RGVQVTPQDF PISKKGLGS
	Isoform b [UniParc]. Checksum: FCBA3B352F1F0952	FASTA	294	32,507
	10 20 30 40 50 60 MPSAGLCSCW GGRVLPLLLA YVCYLLLGAT IFQLLERQAE AQSRDQFQLE KLRFLENYTC 70 80 90 100 110 120 LDQWAMEQFV QVIMEAWVKG VNPKGNSTNP SNWDFGSSFF FAGTVVTTIG YGNLAPSTEA 130 140 150 160 170 180 GQVFCVFYAL LGIPLNVIFL NHLGTGLRAH LAAIERWEDR PRRSQVLQVL GLALFLTLGT 190 200 210 220 230 240 LVILIFPPMV FSHVEGWSFS EGFYFAFITL STIGFGDYVV GTDPSKHYIS VYRSLAAIWI 250 260 270 280 290 LLGLAWLALI LPLGPLLLHR CCQLWLLSRG LGVKDGAASD PSGLPRPQKI PISA
	Isoform c [UniParc]. Checksum: 58B8EA53875CD2F5	FASTA	322	35,802
	10 20 30 40 50 60 MPSAGLCSCW GGRVLPLLLA YVCYLLLGAT IFQLLERQAE AQSRDQFQLE KLRFLENYTC 70 80 90 100 110 120 LDQWAMEQFV QVIMEAWVKG VNPKGNSTNP SNWDFGSSFF FAGTVVTTIG YGNLAPSTEA 130 140 150 160 170 180 GQVFCVFYAL LGIPLNVIFL NHLGTGLRAH LAAIERWEDR PRRSQVLQVL GLALFLTLGT 190 200 210 220 230 240 LVILIFPPMV FSHVEGWSFS EGFYFAFITL STIGFGDYVV GHPLNFITPS GLLPSQEPFQ 250 260 270 280 290 300 TPHGKPESQQ IPGSFQKVSS MNVWPLSGMH SPGLAFPLPD CNIPDQERFR PLHPGAWKFW 310 320 PLPLPSSNSK WAPMWLGSSA QV
	Isoform d [UniParc]. Checksum: 56C1DF119F5931F3	FASTA	262	28,828
	10 20 30 40 50 60 MPSAGLCSCW GGRVLPLLLA YVCYLLLGAT IFQLLERQAE AQSRDQFQLE KLRFLENYTC 70 80 90 100 110 120 LDQWAMEQFV QVIMEAWVKG VNPKGNSTNP SNWDFGSSFF FAGTVVTTIG YGNLAPSTEA 130 140 150 160 170 180 GQVFCVFYAL LGIPLNVIFL NHLGTGLRAH LAAIERWEDR PRRSQVLQVL GLALFLTLGT 190 200 210 220 230 240 LVILIFPPMV FSHVEGWSFS EGFYFAFITL STIGFGDYVV GLRQGCGAKA APGRRPRRGS 250 260 TAARGVQVTP QDFPISKKGL GS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genomic and functional characteristics of novel human pancreatic 2P domain K(+) channels." Girard C., Duprat F., Terrenoire C., Tinel N., Fosset M., Romey G., Lazdunski M., Lesage F. Biochem. Biophys. Res. Commun. 282:249-256(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A). Tissue: Pancreas.
[2]	"Functional properties of four splice variants of a human pancreatic tandem-pore K+ channel, TALK-1." Han J., Kang D., Kim D. Am. J. Physiol. 285:C529-C538(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS B; C AND D), TISSUE SPECIFICITY, ALTERNATIVE SPLICING, VARIANT HIS-301.
[3]	"Regulation of two-pore-domain (K2P) potassium leak channels by the tyrosine kinase inhibitor genistein." Gierten J., Ficker E., Bloehs R., Schlomer K., Kathofer S., Scholz E., Zitron E., Kiesecker C., Bauer A., Becker R., Katus H.A., Karle C.A., Thomas D. Br. J. Pharmacol. 154:1680-1690(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B). Tissue: Pancreas.
[4]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT HIS-301.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C).

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF358909 mRNA. Translation: AAK49532.1. AY253145 mRNA. Translation: AAP82866.1. AY253146 mRNA. Translation: AAP82867.1. AY253147 mRNA. Translation: AAP82868.1. EU978943 mRNA. Translation: ACH86102.1. AL136087 Genomic DNA. Translation: CAI19537.1. CH471081 Genomic DNA. Translation: EAX03982.1. CH471081 Genomic DNA. Translation: EAX03984.1. CH471081 Genomic DNA. Translation: EAX03985.1. BC111860 mRNA. Translation: AAI11861.1.
IPI	IPI00045912. IPI00451446. IPI00451448. IPI00844272.
RefSeq	NP_001128577.1. NM_001135105.1. NP_001128578.1. NM_001135106.1. NP_001128579.1. NM_001135107.1. NP_115491.1. NM_032115.3.
UniGene	Hs.287765.
3D structure databases
ProteinModelPortal	Q96T55.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000391498.
Polymorphism databases
DMDM	24636281.
Proteomic databases
PRIDE	Q96T55.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000373227; ENSP00000362324; ENSG00000095981. ENST00000373229; ENSP00000362326; ENSG00000095981. ENST00000425054; ENSP00000391498; ENSG00000095981. ENST00000437525; ENSP00000415375; ENSG00000095981.
GeneID	83795.
KEGG	hsa:83795.
UCSC	uc003ooq.3. human. uc003oor.4. human. uc010jwy.3. human.
Organism-specific databases
CTD	83795.
GeneCards	GC06M039282.
HGNC	HGNC:14464. KCNK16.
HPA	HPA016567.
MIM	607369. gene.
neXtProt	NX_Q96T55.
PharmGKB	PA30057.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG245381.
HOVERGEN	HBG104884.
InParanoid	Q96T55.
KO	K04924.
OMA	QPRIHIP.
OrthoDB	EOG4N04G4.
PhylomeDB	Q96T55.
Enzyme and pathway databases
Reactome	REACT_13685. Neuronal System.
Gene expression databases
ArrayExpress	Q96T55.
Bgee	Q96T55.
CleanEx	HS_KCNK16.
Genevestigator	Q96T55.
GermOnline	ENSG00000095981. Homo sapiens.
Family and domain databases
InterPro	IPR003280. 2pore_dom_K_chnl. IPR003092. 2pore_dom_K_chnl_TASK. IPR013099. Ion_trans_2. [Graphical view]
Pfam	PF07885. Ion_trans_2. 2 hits. [Graphical view]
PRINTS	PR01333. 2POREKCHANEL. PR01095. TASKCHANNEL.
ProtoNet	Search...
Other
GenomeRNAi	83795.
NextBio	72807.
SOURCE	Search...

Entry information

Entry name

KCNKG_HUMAN

Accession

Primary (citable) accession number: Q96T55
Secondary accession number(s): B5TJL9 , Q2M2N9, Q5TCF3, Q6X6Z3, Q6X6Z4, Q6X6Z5, Q9H591

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.