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Q96T53 (MBOA4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ghrelin O-acyltransferase

EC=2.3.1.-
Alternative name(s):
Membrane-bound O-acyltransferase domain-containing protein 4
Short name=O-acyltransferase domain-containing protein 4
Gene names
Name:MBOAT4
Synonyms:GOAT, OACT4
ORF Names:FKSG89
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length435 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates the octanoylation of ghrelin at 'Ser-3'. Can use a variety of fatty acids as substrates including octanoic acid, decanoic acid and tetradecanoic acid. Ref.1

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Expressed predominantly in stomach with moderate levels in pancreas and relatively low levels in most other tissues. Ref.1

Sequence similarities

Belongs to the membrane-bound acyltransferase family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96T53-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96T53-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-106: Missing.
     107-114: YLHEPPSV → MFFKKLSC
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 435435Ghrelin O-acyltransferase
PRO_0000273024

Regions

Transmembrane6 – 2621Helical; Potential
Transmembrane43 – 6321Helical; Potential
Transmembrane163 – 18321Helical; Potential
Transmembrane241 – 26121Helical; Potential
Transmembrane345 – 36521Helical; Potential
Transmembrane377 – 39721Helical; Potential
Transmembrane407 – 42721Helical; Potential

Sites

Active site3071 By similarity
Active site3381 Probable

Natural variations

Alternative sequence1 – 106106Missing in isoform 2.
VSP_032958
Alternative sequence107 – 1148YLHEPPSV → MFFKKLSC in isoform 2.
VSP_032959
Natural variant461T → A. Ref.1
Corresponds to variant rs7813902 [ dbSNP | Ensembl ].
VAR_059434
Natural variant2311G → E.
Corresponds to variant rs16876563 [ dbSNP | Ensembl ].
VAR_030069

Experimental info

Mutagenesis3381H → A: Abolishes ability to acylate ghrelin. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 8, 2008. Version 2.
Checksum: 0CF97AAE734F24A7

FASTA43549,716
        10         20         30         40         50         60 
MEWLWLFFLH PISFYQGAAF PFALLFNYLC IMDSFSTRAR YLFLLTGGGA LAVAAMGSYA 

        70         80         90        100        110        120 
VLVFTPAVCA VALLCSLAPQ QVHRWTFCFQ MSWQTLCHLG LHYTEYYLHE PPSVRFCITL 

       130        140        150        160        170        180 
SSLMLLTQRV TSLSLDICEG KVKAASGGFR SRSSLSEHVC KALPYFSYLL FFPALLGGSL 

       190        200        210        220        230        240 
CSFQRFQARV QGSSALHPRH SFWALSWRGL QILGLECLNV AVSRVVDAGA GLTDCQQFEC 

       250        260        270        280        290        300 
IYVVWTTAGL FKLTYYSHWI LDDSLLHAAG FGPELGQSPG EEGYVPDADI WTLERTHRIS 

       310        320        330        340        350        360 
VFSRKWNQST ARWLRRLVFQ HSRAWPLLQT FAFSAWWHGL HPGQVFGFVC WAVMVEADYL 

       370        380        390        400        410        420 
IHSFANEFIR SWPMRLFYRT LTWAHTQLII AYIMLAVEVR SLSSLWLLCN SYNSVFPMVY 

       430 
CILLLLLAKR KHKCN 

« Hide

Isoform 2 [UniParc].

Checksum: E1A0D73A81BF9C18
Show »

FASTA32937,721

References

« Hide 'large scale' references
[1]"Ghrelin octanoylation mediated by an orphan lipid transferase."
Gutierrez J.A., Solenberg P.J., Perkins D.R., Willency J.A., Knierman M.D., Jin Z., Witcher D.R., Luo S., Onyia J.E., Hale J.E.
Proc. Natl. Acad. Sci. U.S.A. 105:6320-6325(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF HIS-338, VARIANT ALA-46.
Tissue: Thyroid carcinoma.
[2]"Identification of FKSG89, a novel gene located on human chromosome 8."
Wang Y.-G., Gong L.
Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]"DNA sequence and analysis of human chromosome 8."
Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. expand/collapse author list , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EU518495 mRNA. Translation: ACB05873.2.
AF359269 mRNA. Translation: AAK43717.1.
AC026979 Genomic DNA. No translation available.
CCDSCCDS47835.1. [Q96T53-1]
RefSeqNP_001094386.1. NM_001100916.1. [Q96T53-1]
UniGeneHs.627194.

3D structure databases

ProteinModelPortalQ96T53.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000314196.

PTM databases

PhosphoSiteQ96T53.

Polymorphism databases

DMDM182676418.

Proteomic databases

PaxDbQ96T53.
PRIDEQ96T53.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000320542; ENSP00000314196; ENSG00000177669. [Q96T53-1]
GeneID619373.
KEGGhsa:619373.
UCSCuc010lvg.3. human. [Q96T53-1]

Organism-specific databases

CTD619373.
GeneCardsGC08M029989.
H-InvDBHIX0034281.
HGNCHGNC:32311. MBOAT4.
MIM611940. gene.
neXtProtNX_Q96T53.
PharmGKBPA142671233.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG268942.
HOGENOMHOG000113492.
HOVERGENHBG108120.
InParanoidQ96T53.
OMAAFSAWWH.
OrthoDBEOG74TWZX.
PhylomeDBQ96T53.
TreeFamTF314906.

Enzyme and pathway databases

ReactomeREACT_17015. Metabolism of proteins.

Gene expression databases

CleanExHS_MBOAT4.
GenevestigatorQ96T53.

Family and domain databases

InterProIPR004299. MBOAT_fam.
[Graphical view]
PfamPF03062. MBOAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi619373.
NextBio112914.
PROQ96T53.
SOURCESearch...

Entry information

Entry nameMBOA4_HUMAN
AccessionPrimary (citable) accession number: Q96T53
Secondary accession number(s): B1Q003
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: April 8, 2008
Last modified: July 9, 2014
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM