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Q96T53

- MBOA4_HUMAN

UniProt

Q96T53 - MBOA4_HUMAN

Protein

Ghrelin O-acyltransferase

Gene

MBOAT4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 2 (08 Apr 2008)
      Previous versions | rss
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    Functioni

    Mediates the octanoylation of ghrelin at 'Ser-3'. Can use a variety of fatty acids as substrates including octanoic acid, decanoic acid and tetradecanoic acid.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei307 – 3071By similarity
    Active sitei338 – 3381Curated

    GO - Molecular functioni

    1. serine O-acyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. cellular protein metabolic process Source: Reactome
    2. peptidyl-serine octanoylation Source: UniProtKB

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Enzyme and pathway databases

    ReactomeiREACT_19189. Synthesis, secretion, and deacylation of Ghrelin.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ghrelin O-acyltransferase (EC:2.3.1.-)
    Alternative name(s):
    Membrane-bound O-acyltransferase domain-containing protein 4
    Short name:
    O-acyltransferase domain-containing protein 4
    Gene namesi
    Name:MBOAT4
    Synonyms:GOAT, OACT4
    ORF Names:FKSG89
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:32311. MBOAT4.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: Reactome
    2. integral component of endoplasmic reticulum membrane Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi338 – 3381H → A: Abolishes ability to acylate ghrelin. 1 Publication

    Organism-specific databases

    PharmGKBiPA142671233.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 435435Ghrelin O-acyltransferasePRO_0000273024Add
    BLAST

    Proteomic databases

    PaxDbiQ96T53.
    PRIDEiQ96T53.

    PTM databases

    PhosphoSiteiQ96T53.

    Expressioni

    Tissue specificityi

    Expressed predominantly in stomach with moderate levels in pancreas and relatively low levels in most other tissues.1 Publication

    Gene expression databases

    CleanExiHS_MBOAT4.
    GenevestigatoriQ96T53.

    Interactioni

    Protein-protein interaction databases

    STRINGi9606.ENSP00000314196.

    Structurei

    3D structure databases

    ProteinModelPortaliQ96T53.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei6 – 2621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei43 – 6321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei163 – 18321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei241 – 26121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei345 – 36521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei377 – 39721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei407 – 42721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG268942.
    HOGENOMiHOG000113492.
    HOVERGENiHBG108120.
    InParanoidiQ96T53.
    OMAiAFSAWWH.
    OrthoDBiEOG74TWZX.
    PhylomeDBiQ96T53.
    TreeFamiTF314906.

    Family and domain databases

    InterProiIPR004299. MBOAT_fam.
    [Graphical view]
    PfamiPF03062. MBOAT. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q96T53-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEWLWLFFLH PISFYQGAAF PFALLFNYLC IMDSFSTRAR YLFLLTGGGA    50
    LAVAAMGSYA VLVFTPAVCA VALLCSLAPQ QVHRWTFCFQ MSWQTLCHLG 100
    LHYTEYYLHE PPSVRFCITL SSLMLLTQRV TSLSLDICEG KVKAASGGFR 150
    SRSSLSEHVC KALPYFSYLL FFPALLGGSL CSFQRFQARV QGSSALHPRH 200
    SFWALSWRGL QILGLECLNV AVSRVVDAGA GLTDCQQFEC IYVVWTTAGL 250
    FKLTYYSHWI LDDSLLHAAG FGPELGQSPG EEGYVPDADI WTLERTHRIS 300
    VFSRKWNQST ARWLRRLVFQ HSRAWPLLQT FAFSAWWHGL HPGQVFGFVC 350
    WAVMVEADYL IHSFANEFIR SWPMRLFYRT LTWAHTQLII AYIMLAVEVR 400
    SLSSLWLLCN SYNSVFPMVY CILLLLLAKR KHKCN 435
    Length:435
    Mass (Da):49,716
    Last modified:April 8, 2008 - v2
    Checksum:i0CF97AAE734F24A7
    GO
    Isoform 2 (identifier: Q96T53-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-106: Missing.
         107-114: YLHEPPSV → MFFKKLSC

    Note: No experimental confirmation available.

    Show »
    Length:329
    Mass (Da):37,721
    Checksum:iE1A0D73A81BF9C18
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti46 – 461T → A.1 Publication
    Corresponds to variant rs7813902 [ dbSNP | Ensembl ].
    VAR_059434
    Natural varianti231 – 2311G → E.
    Corresponds to variant rs16876563 [ dbSNP | Ensembl ].
    VAR_030069

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 106106Missing in isoform 2. 1 PublicationVSP_032958Add
    BLAST
    Alternative sequencei107 – 1148YLHEPPSV → MFFKKLSC in isoform 2. 1 PublicationVSP_032959

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EU518495 mRNA. Translation: ACB05873.2.
    AF359269 mRNA. Translation: AAK43717.1.
    AC026979 Genomic DNA. No translation available.
    CCDSiCCDS47835.1. [Q96T53-1]
    RefSeqiNP_001094386.1. NM_001100916.1. [Q96T53-1]
    UniGeneiHs.627194.

    Genome annotation databases

    EnsembliENST00000320542; ENSP00000314196; ENSG00000177669. [Q96T53-1]
    GeneIDi619373.
    KEGGihsa:619373.
    UCSCiuc010lvg.3. human. [Q96T53-1]

    Polymorphism databases

    DMDMi182676418.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EU518495 mRNA. Translation: ACB05873.2 .
    AF359269 mRNA. Translation: AAK43717.1 .
    AC026979 Genomic DNA. No translation available.
    CCDSi CCDS47835.1. [Q96T53-1 ]
    RefSeqi NP_001094386.1. NM_001100916.1. [Q96T53-1 ]
    UniGenei Hs.627194.

    3D structure databases

    ProteinModelPortali Q96T53.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000314196.

    PTM databases

    PhosphoSitei Q96T53.

    Polymorphism databases

    DMDMi 182676418.

    Proteomic databases

    PaxDbi Q96T53.
    PRIDEi Q96T53.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000320542 ; ENSP00000314196 ; ENSG00000177669 . [Q96T53-1 ]
    GeneIDi 619373.
    KEGGi hsa:619373.
    UCSCi uc010lvg.3. human. [Q96T53-1 ]

    Organism-specific databases

    CTDi 619373.
    GeneCardsi GC08M029989.
    H-InvDB HIX0034281.
    HGNCi HGNC:32311. MBOAT4.
    MIMi 611940. gene.
    neXtProti NX_Q96T53.
    PharmGKBi PA142671233.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG268942.
    HOGENOMi HOG000113492.
    HOVERGENi HBG108120.
    InParanoidi Q96T53.
    OMAi AFSAWWH.
    OrthoDBi EOG74TWZX.
    PhylomeDBi Q96T53.
    TreeFami TF314906.

    Enzyme and pathway databases

    Reactomei REACT_19189. Synthesis, secretion, and deacylation of Ghrelin.

    Miscellaneous databases

    GenomeRNAii 619373.
    NextBioi 112914.
    PROi Q96T53.
    SOURCEi Search...

    Gene expression databases

    CleanExi HS_MBOAT4.
    Genevestigatori Q96T53.

    Family and domain databases

    InterProi IPR004299. MBOAT_fam.
    [Graphical view ]
    Pfami PF03062. MBOAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF HIS-338, VARIANT ALA-46.
      Tissue: Thyroid carcinoma.
    2. "Identification of FKSG89, a novel gene located on human chromosome 8."
      Wang Y.-G., Gong L.
      Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    3. "DNA sequence and analysis of human chromosome 8."
      Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
      , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
      Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiMBOA4_HUMAN
    AccessioniPrimary (citable) accession number: Q96T53
    Secondary accession number(s): B1Q003
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2007
    Last sequence update: April 8, 2008
    Last modified: October 1, 2014
    This is version 71 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3