Q96T53 (MBOA4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ghrelin O-acyltransferase
EC=2.3.1.-

Alternative name(s):
Membrane-bound O-acyltransferase domain-containing protein 4
Short name=O-acyltransferase domain-containing protein 4

Gene names

Name:	MBOAT4
Synonyms:	GOAT, OACT4
ORF Names:	FKSG89

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates the octanoylation of ghrelin at 'Ser-3'. Can use a variety of fatty acids as substrates including octanoic acid, decanoic acid and tetradecanoic acid. Ref.1
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Expressed predominantly in stomach with moderate levels in pancreas and relatively low levels in most other tissues. Ref.1
Sequence similarities	Belongs to the membrane-bound acyltransferase family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	peptidyl-serine octanoylation Inferred from direct assay Ref.1. Source: UniProtKB
Cellular_component	integral to endoplasmic reticulum membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	serine O-acyltransferase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96T53-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96T53-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-106: Missing.
     107-114: YLHEPPSV → MFFKKLSC

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 435	435	Ghrelin O-acyltransferase		PRO_0000273024
Regions
	Transmembrane	6 – 26	21	Helical; Potential
	Transmembrane	43 – 63	21	Helical; Potential
	Transmembrane	163 – 183	21	Helical; Potential
	Transmembrane	241 – 261	21	Helical; Potential
	Transmembrane	345 – 365	21	Helical; Potential
	Transmembrane	377 – 397	21	Helical; Potential
	Transmembrane	407 – 427	21	Helical; Potential
	Compositional bias	423 – 427	5	Poly-Leu
Sites
	Active site	307	1	By similarity
	Active site	338	1	Probable
Natural variations
	Alternative sequence	1 – 106	106	Missing in isoform 2.		VSP_032958
	Alternative sequence	107 – 114	8	YLHEPPSV → MFFKKLSC in isoform 2.		VSP_032959
	Natural variant	46	1	T → A. Ref.1 Corresponds to variant rs7813902 [ dbSNP | Ensembl ].		VAR_059434
	Natural variant	231	1	G → E. Corresponds to variant rs16876563 [ dbSNP | Ensembl ].		VAR_030069
Experimental info
	Mutagenesis	338	1	H → A: Abolishes ability to acylate ghrelin. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 8, 2008. Version 2. Checksum: 0CF97AAE734F24A7	FASTA	435	49,716
	10 20 30 40 50 60 MEWLWLFFLH PISFYQGAAF PFALLFNYLC IMDSFSTRAR YLFLLTGGGA LAVAAMGSYA 70 80 90 100 110 120 VLVFTPAVCA VALLCSLAPQ QVHRWTFCFQ MSWQTLCHLG LHYTEYYLHE PPSVRFCITL 130 140 150 160 170 180 SSLMLLTQRV TSLSLDICEG KVKAASGGFR SRSSLSEHVC KALPYFSYLL FFPALLGGSL 190 200 210 220 230 240 CSFQRFQARV QGSSALHPRH SFWALSWRGL QILGLECLNV AVSRVVDAGA GLTDCQQFEC 250 260 270 280 290 300 IYVVWTTAGL FKLTYYSHWI LDDSLLHAAG FGPELGQSPG EEGYVPDADI WTLERTHRIS 310 320 330 340 350 360 VFSRKWNQST ARWLRRLVFQ HSRAWPLLQT FAFSAWWHGL HPGQVFGFVC WAVMVEADYL 370 380 390 400 410 420 IHSFANEFIR SWPMRLFYRT LTWAHTQLII AYIMLAVEVR SLSSLWLLCN SYNSVFPMVY 430 CILLLLLAKR KHKCN
	Isoform 2 [UniParc]. Checksum: E1A0D73A81BF9C18	FASTA	329	37,721
	10 20 30 40 50 60 MFFKKLSCRF CITLSSLMLL TQRVTSLSLD ICEGKVKAAS GGFRSRSSLS EHVCKALPYF 70 80 90 100 110 120 SYLLFFPALL GGSLCSFQRF QARVQGSSAL HPRHSFWALS WRGLQILGLE CLNVAVSRVV 130 140 150 160 170 180 DAGAGLTDCQ QFECIYVVWT TAGLFKLTYY SHWILDDSLL HAAGFGPELG QSPGEEGYVP 190 200 210 220 230 240 DADIWTLERT HRISVFSRKW NQSTARWLRR LVFQHSRAWP LLQTFAFSAW WHGLHPGQVF 250 260 270 280 290 300 GFVCWAVMVE ADYLIHSFAN EFIRSWPMRL FYRTLTWAHT QLIIAYIMLA VEVRSLSSLW 310 320 LLCNSYNSVF PMVYCILLLL LAKRKHKCN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ghrelin octanoylation mediated by an orphan lipid transferase." Gutierrez J.A., Solenberg P.J., Perkins D.R., Willency J.A., Knierman M.D., Jin Z., Witcher D.R., Luo S., Onyia J.E., Hale J.E. Proc. Natl. Acad. Sci. U.S.A. 105:6320-6325(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF HIS-338, VARIANT ALA-46. Tissue: Thyroid carcinoma.
[2]	"Identification of FKSG89, a novel gene located on human chromosome 8." Wang Y.-G., Gong L. Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]	"DNA sequence and analysis of human chromosome 8." Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S. Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EU518495 mRNA. Translation: ACB05873.2. AF359269 mRNA. Translation: AAK43717.1. AC026979 Genomic DNA. No translation available.
IPI	IPI00045909. IPI00896483.
RefSeq	NP_001094386.1. NM_001100916.1.
UniGene	Hs.627194.
3D structure databases
ProteinModelPortal	Q96T53.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000314196.
PTM databases
PhosphoSite	Q96T53.
Polymorphism databases
DMDM	182676418.
Proteomic databases
PaxDb	Q96T53.
PRIDE	Q96T53.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000320542; ENSP00000314196; ENSG00000177669.
GeneID	619373.
KEGG	hsa:619373.
UCSC	uc010lvg.3. human.
Organism-specific databases
CTD	619373.
GeneCards	GC08M029989.
H-InvDB	HIX0034281.
HGNC	HGNC:32311. MBOAT4.
MIM	611940. gene.
neXtProt	NX_Q96T53.
PharmGKB	PA142671233.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG268942.
HOGENOM	HOG000113492.
HOVERGEN	HBG108120.
InParanoid	Q96T53.
OMA	AFSAWWH.
OrthoDB	EOG4PRSQQ.
Enzyme and pathway databases
Reactome	REACT_116125. Disease.
Gene expression databases
CleanEx	HS_MBOAT4.
Genevestigator	Q96T53.
Family and domain databases
InterPro	IPR004299. MBOAT_fam. [Graphical view]
Pfam	PF03062. MBOAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	619373.
NextBio	112914.
SOURCE	Search...

Entry information

Entry name

MBOA4_HUMAN

Accession

Primary (citable) accession number: Q96T53
Secondary accession number(s): B1Q003

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	April 8, 2008
Last modified:	April 3, 2013
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.