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Protein

Protein phosphatase 1 regulatory inhibitor subunit 16B

Gene

PPP1R16B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulator of protein phosphatase 1 (PP1) that acts as a positive regulator of pulmonary endothelial cell (EC) barrier function (PubMed:18586956). Involved in the regulation of the PI3K/AKT signaling pathway, angiogenesis and endothelial cell proliferation (PubMed:25007873). Regulates angiogenesis and endothelial cell proliferation through the control of ECE1 dephosphorylation, trafficking and activity (By similarity). Protects the endothelial barrier from lipopolysaccharide (LPS)-induced vascular leakage (By similarity). Involved in the regulation of endothelial cell filopodia extension (By similarity). May be a downstream target for TGF-beta1 signaling cascade in endothelial cells (PubMed:16263087, PubMed:18586956). Involved in PKA-mediated moesin dephosphorylation which is important in EC barrier protection against thrombin stimulation (PubMed:18586956). Promotes the interaction of PPP1CA with RPSA/LAMR1 and in turn facilitates the dephosphorylation of RPSA/LAMR1 (PubMed:16263087). Involved in the dephosphorylation of EEF1A1 (PubMed:26497934).By similarity4 Publications

GO - Molecular functioni

  • protein phosphatase regulator activity Source: GO_Central

GO - Biological processi

  • establishment of endothelial barrier Source: GO_Central
  • negative regulation of peptidyl-serine dephosphorylation Source: GO_Central
  • negative regulation of protein dephosphorylation Source: GO_Central
  • positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis Source: GO_Central
  • positive regulation of endothelial cell proliferation Source: GO_Central
  • positive regulation of protein dephosphorylation Source: GO_Central
  • regulation of filopodium assembly Source: UniProtKB
  • regulation of phosphatidylinositol 3-kinase signaling Source: GO_Central
  • regulation of protein dephosphorylation Source: UniProtKB

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1 regulatory inhibitor subunit 16B
Alternative name(s):
Ankyrin repeat domain-containing protein 4
CAAX box protein TIMAP
TGF-beta-inhibited membrane-associated protein
Short name:
hTIMAP
Gene namesi
Name:PPP1R16B
Synonyms:ANKRD4, KIAA0823
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000101445.9
HGNCiHGNC:15850 PPP1R16B
MIMi613275 gene
neXtProtiNX_Q96T49

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi252G → A: Strongly decreased interaction with EEF1A1. 1 Publication1

Organism-specific databases

DisGeNETi26051
OpenTargetsiENSG00000101445
PharmGKBiPA33635

Polymorphism and mutation databases

BioMutaiPPP1R16B
DMDMi22256977

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000670431 – 564Protein phosphatase 1 regulatory inhibitor subunit 16BAdd BLAST564
PropeptideiPRO_0000396710565 – 567Removed in mature formSequence analysis3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei69PhosphoserineBy similarity1
Modified residuei333PhosphoserineBy similarity1
Modified residuei337PhosphoserineBy similarity1
Modified residuei350PhosphoserineCombined sources1
Modified residuei476PhosphoserineCombined sources1
Lipidationi563S-palmitoyl cysteineBy similarity1
Modified residuei564Cysteine methyl esterBy similarity1
Lipidationi564S-farnesyl cysteineBy similarity1

Post-translational modificationi

Phosphorylated by PKA and, after PKA priming, by GSK3B. Phosphorylation by GSK3B reduces its association with PP1C and enhances PP1C activity. Dephosphorylation by its associated PP1C results in enhanced association with PP1C, but reduced PP1C activity (By similarity).By similarity

Keywords - PTMi

Lipoprotein, Methylation, Palmitate, Phosphoprotein, Prenylation

Proteomic databases

MaxQBiQ96T49
PaxDbiQ96T49
PeptideAtlasiQ96T49
PRIDEiQ96T49

PTM databases

iPTMnetiQ96T49
PhosphoSitePlusiQ96T49
SwissPalmiQ96T49

Expressioni

Inductioni

Inhibited by TGFB1 (Probable). Down-regulated by LPS (PubMed:21907835).Curated1 Publication

Gene expression databases

BgeeiENSG00000101445
CleanExiHS_PPP1R16B
GenevisibleiQ96T49 HS

Organism-specific databases

HPAiHPA049571

Interactioni

Subunit structurei

Interacts with PPP1CA, PPP1CB and MSN. Interacts (via its fourth ankyrin repeat) with the mature dimeric form of RPSA/LAMR1 (PubMed:16263087, PubMed:18586956). Interacts with EEF1A1 (PubMed:26497934). Interacts with PTEN (PubMed:25007873). Interacts with ECE1 (By similarity).By similarity4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi117514, 26 interactors
ELMiQ96T49
IntActiQ96T49, 29 interactors
MINTiQ96T49
STRINGi9606.ENSP00000299824

Structurei

3D structure databases

ProteinModelPortaliQ96T49
SMRiQ96T49
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati100 – 129ANK 1Add BLAST30
Repeati133 – 162ANK 2Add BLAST30
Repeati228 – 257ANK 3Add BLAST30
Repeati261 – 290ANK 4Add BLAST30
Repeati530 – 559ANK 5Add BLAST30

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili15 – 55Sequence analysisAdd BLAST41

Keywords - Domaini

ANK repeat, Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0505 Eukaryota
COG0666 LUCA
GeneTreeiENSGT00760000119141
HOGENOMiHOG000236276
HOVERGENiHBG053644
InParanoidiQ96T49
KOiK17459
OMAiATCGHIN
OrthoDBiEOG091G06S2
PhylomeDBiQ96T49
TreeFamiTF316803

Family and domain databases

CDDicd00204 ANK, 2 hits
Gene3Di1.25.40.20, 2 hits
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR017417 Pase-1_reg_su_16AB
PfamiView protein in Pfam
PF12796 Ank_2, 2 hits
PIRSFiPIRSF038159 PP1_16AB_vert, 1 hit
SMARTiView protein in SMART
SM00248 ANK, 5 hits
SUPFAMiSSF48403 SSF48403, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 4 hits

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96T49-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASHVDLLTE LQLLEKVPTL ERLRAAQKRR AQQLKKWAQY EQDLQHRKRK
60 70 80 90 100
HERKRSTGGR RKKVSFEASV ALLEASLRND AEEVRYFLKN KVSPDLCNED
110 120 130 140 150
GLTALHQCCI DNFEEIVKLL LSHGANVNAK DNELWTPLHA AATCGHINLV
160 170 180 190 200
KILVQYGADL LAVNSDGNMP YDLCEDEPTL DVIETCMAYQ GITQEKINEM
210 220 230 240 250
RVAPEQQMIA DIHCMIAAGQ DLDWIDAQGA TLLHIAGANG YLRAAELLLD
260 270 280 290 300
HGVRVDVKDW DGWEPLHAAA FWGQMQMAEL LVSHGASLSA RTSMDEMPID
310 320 330 340 350
LCEEEEFKVL LLELKHKHDV IMKSQLRHKS SLSRRTSSAG SRGKVVRRAS
360 370 380 390 400
LSDRTNLYRK EYEGEAILWQ RSAAEDQRTS TYNGDIRETR TDQENKDPNP
410 420 430 440 450
RLEKPVLLSE FPTKIPRGEL DMPVENGLRA PVSAYQYALA NGDVWKVHEV
460 470 480 490 500
PDYSMAYGNP GVADATPPWS SYKEQSPQTL LELKRQRAAA KLLSHPFLST
510 520 530 540 550
HLGSSMARTG ESSSEGKAPL IGGRTSPYSS NGTSVYYTVT SGDPPLLKFK
560
APIEEMEEKV HGCCRIS
Length:567
Mass (Da):63,551
Last modified:December 1, 2001 - v1
Checksum:i8418CE26D0035CF1
GO
Isoform 2 (identifier: Q96T49-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     233-274: Missing.

Show »
Length:525
Mass (Da):58,900
Checksum:i0904F38A8061CD26
GO

Sequence cautioni

The sequence BAA74846 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_047508233 – 274Missing in isoform 2. 1 PublicationAdd BLAST42

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF362910 mRNA Translation: AAK52796.1
AB020630 mRNA Translation: BAA74846.2 Different initiation.
AL023803 Genomic DNA No translation available.
AL031657 Genomic DNA No translation available.
AL121889 Genomic DNA No translation available.
CH471077 Genomic DNA Translation: EAW76009.1
BC131801 mRNA Translation: AAI31802.1
BC152467 mRNA Translation: AAI52468.1
AB177855 mRNA Translation: BAD66833.1
CCDSiCCDS13309.1 [Q96T49-1]
CCDS54462.1 [Q96T49-2]
RefSeqiNP_001166206.1, NM_001172735.2 [Q96T49-2]
NP_056383.1, NM_015568.3 [Q96T49-1]
UniGeneiHs.45719

Genome annotation databases

EnsembliENST00000299824; ENSP00000299824; ENSG00000101445 [Q96T49-1]
ENST00000373331; ENSP00000362428; ENSG00000101445 [Q96T49-2]
GeneIDi26051
KEGGihsa:26051
UCSCiuc002xje.3 human [Q96T49-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPP16B_HUMAN
AccessioniPrimary (citable) accession number: Q96T49
Secondary accession number(s): A2RRR6
, E9PFS8, O94912, Q5W9G4, Q9NQG4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: December 1, 2001
Last modified: March 28, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health