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Protein

Protein phosphatase 1 regulatory inhibitor subunit 16B

Gene

PPP1R16B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulator of protein phosphatase 1 (PP1) that acts as a positive regulator of pulmonary endothelial cell (EC) barrier function. Involved in PKA-mediated moesin dephosphorylation which is important in EC barrier protection against thrombin stimulation. Promotes the interaction of PPP1CA with RPSA/LAMR1 and in turn facilitates the dephosphorylation of RPSA/LAMR1. Involved in the regulation of endothelial cell filopodia extension. May be a downstream target for TGF-beta1 signaling cascade in endothelial cells.2 Publications

GO - Molecular functioni

  • protein phosphatase type 1 regulator activity Source: GO_Central

GO - Biological processi

  • establishment of endothelial barrier Source: GO_Central
  • negative regulation of peptidyl-serine dephosphorylation Source: GO_Central
  • negative regulation of protein dephosphorylation Source: GO_Central
  • positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis Source: GO_Central
  • positive regulation of endothelial cell proliferation Source: GO_Central
  • positive regulation of protein dephosphorylation Source: GO_Central
  • regulation of filopodium assembly Source: UniProtKB
  • regulation of phosphatidylinositol 3-kinase signaling Source: GO_Central
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1 regulatory inhibitor subunit 16B
Alternative name(s):
Ankyrin repeat domain-containing protein 4
CAAX box protein TIMAP
TGF-beta-inhibited membrane-associated protein
Short name:
hTIMAP
Gene namesi
Name:PPP1R16B
Synonyms:ANKRD4, KIAA0823
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

HGNCiHGNC:15850. PPP1R16B.

Subcellular locationi

GO - Cellular componenti

  • cell projection Source: GO_Central
  • nucleus Source: UniProtKB
  • perinuclear region of cytoplasm Source: UniProtKB
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi26051.
OpenTargetsiENSG00000101445.
PharmGKBiPA33635.

Polymorphism and mutation databases

BioMutaiPPP1R16B.
DMDMi22256977.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000670431 – 564Protein phosphatase 1 regulatory inhibitor subunit 16BAdd BLAST564
PropeptideiPRO_0000396710565 – 567Removed in mature formSequence analysis3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei69PhosphoserineBy similarity1
Modified residuei350PhosphoserineCombined sources1
Modified residuei476PhosphoserineCombined sources1
Lipidationi563S-palmitoyl cysteineBy similarity1
Modified residuei564Cysteine methyl esterBy similarity1
Lipidationi564S-farnesyl cysteineBy similarity1

Post-translational modificationi

Phosphorylated by PKA and, after PKA priming, by GSK3B. Phosphorylation by GSK3B reduces its association with PP1C and enhances PP1C activity. Dephosphorylation by its associated PP1C results in enhanced association with PP1C, but reduced PP1C activity (By similarity).By similarity

Keywords - PTMi

Lipoprotein, Methylation, Palmitate, Phosphoprotein, Prenylation

Proteomic databases

EPDiQ96T49.
MaxQBiQ96T49.
PaxDbiQ96T49.
PeptideAtlasiQ96T49.
PRIDEiQ96T49.

PTM databases

iPTMnetiQ96T49.
PhosphoSitePlusiQ96T49.
SwissPalmiQ96T49.

Expressioni

Tissue specificityi

Highly expressed in vascular endothelium, CNS, lung, spleen, kidney and testis.

Inductioni

Inhibited by TGFB1.

Gene expression databases

BgeeiENSG00000101445.
CleanExiHS_PPP1R16B.
GenevisibleiQ96T49. HS.

Organism-specific databases

HPAiHPA049571.

Interactioni

Subunit structurei

Interacts with PPP1CA, PPP1CB and MSN. Interacts (via its fourth ankyrin repeat) with the mature dimeric form of RPSA/LAMR1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CDCA7LQ96GN53EBI-10293968,EBI-5278764
CEP55D3DR373EBI-10293968,EBI-10173536
CEP70Q8NHQ13EBI-10293968,EBI-739624
CRXO431863EBI-10293968,EBI-748171
FAM208BQ5VWN6-23EBI-10293968,EBI-10172380
GOLGA2Q083793EBI-10293968,EBI-618309
IKZF3Q9UKT95EBI-10293968,EBI-747204
KCTD9Q7L2733EBI-10293968,EBI-4397613
NEFLI6L9F63EBI-10293968,EBI-10178578
PHC2Q8IXK03EBI-10293968,EBI-713786
SF3B4Q154273EBI-10293968,EBI-348469
TADA2AO754783EBI-10293968,EBI-742268

Protein-protein interaction databases

BioGridi117514. 24 interactors.
IntActiQ96T49. 24 interactors.
STRINGi9606.ENSP00000299824.

Structurei

3D structure databases

ProteinModelPortaliQ96T49.
SMRiQ96T49.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati100 – 129ANK 1Add BLAST30
Repeati133 – 162ANK 2Add BLAST30
Repeati228 – 257ANK 3Add BLAST30
Repeati261 – 290ANK 4Add BLAST30
Repeati530 – 559ANK 5Add BLAST30

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili15 – 55Sequence analysisAdd BLAST41

Sequence similaritiesi

Contains 5 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0505. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00760000119141.
HOGENOMiHOG000236276.
HOVERGENiHBG053644.
InParanoidiQ96T49.
KOiK17459.
OMAiNAKDNEL.
OrthoDBiEOG091G06S2.
PhylomeDBiQ96T49.
TreeFamiTF316803.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR017417. Pase-1_reg_su_16AB.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
[Graphical view]
PIRSFiPIRSF038159. PP1_16AB_vert. 1 hit.
SMARTiSM00248. ANK. 5 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96T49-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASHVDLLTE LQLLEKVPTL ERLRAAQKRR AQQLKKWAQY EQDLQHRKRK
60 70 80 90 100
HERKRSTGGR RKKVSFEASV ALLEASLRND AEEVRYFLKN KVSPDLCNED
110 120 130 140 150
GLTALHQCCI DNFEEIVKLL LSHGANVNAK DNELWTPLHA AATCGHINLV
160 170 180 190 200
KILVQYGADL LAVNSDGNMP YDLCEDEPTL DVIETCMAYQ GITQEKINEM
210 220 230 240 250
RVAPEQQMIA DIHCMIAAGQ DLDWIDAQGA TLLHIAGANG YLRAAELLLD
260 270 280 290 300
HGVRVDVKDW DGWEPLHAAA FWGQMQMAEL LVSHGASLSA RTSMDEMPID
310 320 330 340 350
LCEEEEFKVL LLELKHKHDV IMKSQLRHKS SLSRRTSSAG SRGKVVRRAS
360 370 380 390 400
LSDRTNLYRK EYEGEAILWQ RSAAEDQRTS TYNGDIRETR TDQENKDPNP
410 420 430 440 450
RLEKPVLLSE FPTKIPRGEL DMPVENGLRA PVSAYQYALA NGDVWKVHEV
460 470 480 490 500
PDYSMAYGNP GVADATPPWS SYKEQSPQTL LELKRQRAAA KLLSHPFLST
510 520 530 540 550
HLGSSMARTG ESSSEGKAPL IGGRTSPYSS NGTSVYYTVT SGDPPLLKFK
560
APIEEMEEKV HGCCRIS
Length:567
Mass (Da):63,551
Last modified:December 1, 2001 - v1
Checksum:i8418CE26D0035CF1
GO
Isoform 2 (identifier: Q96T49-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     233-274: Missing.

Show »
Length:525
Mass (Da):58,900
Checksum:i0904F38A8061CD26
GO

Sequence cautioni

The sequence BAA74846 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_047508233 – 274Missing in isoform 2. 1 PublicationAdd BLAST42

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF362910 mRNA. Translation: AAK52796.1.
AB020630 mRNA. Translation: BAA74846.2. Different initiation.
AL023803 Genomic DNA. No translation available.
AL031657, AL121889 Genomic DNA. Translation: CAI20058.1.
AL121889, AL031657 Genomic DNA. Translation: CAI19080.1.
CH471077 Genomic DNA. Translation: EAW76009.1.
BC131801 mRNA. Translation: AAI31802.1.
BC152467 mRNA. Translation: AAI52468.1.
AB177855 mRNA. Translation: BAD66833.1.
CCDSiCCDS13309.1. [Q96T49-1]
CCDS54462.1. [Q96T49-2]
RefSeqiNP_001166206.1. NM_001172735.2. [Q96T49-2]
NP_056383.1. NM_015568.3. [Q96T49-1]
UniGeneiHs.45719.

Genome annotation databases

EnsembliENST00000299824; ENSP00000299824; ENSG00000101445. [Q96T49-1]
ENST00000373331; ENSP00000362428; ENSG00000101445. [Q96T49-2]
GeneIDi26051.
KEGGihsa:26051.
UCSCiuc002xje.3. human. [Q96T49-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF362910 mRNA. Translation: AAK52796.1.
AB020630 mRNA. Translation: BAA74846.2. Different initiation.
AL023803 Genomic DNA. No translation available.
AL031657, AL121889 Genomic DNA. Translation: CAI20058.1.
AL121889, AL031657 Genomic DNA. Translation: CAI19080.1.
CH471077 Genomic DNA. Translation: EAW76009.1.
BC131801 mRNA. Translation: AAI31802.1.
BC152467 mRNA. Translation: AAI52468.1.
AB177855 mRNA. Translation: BAD66833.1.
CCDSiCCDS13309.1. [Q96T49-1]
CCDS54462.1. [Q96T49-2]
RefSeqiNP_001166206.1. NM_001172735.2. [Q96T49-2]
NP_056383.1. NM_015568.3. [Q96T49-1]
UniGeneiHs.45719.

3D structure databases

ProteinModelPortaliQ96T49.
SMRiQ96T49.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117514. 24 interactors.
IntActiQ96T49. 24 interactors.
STRINGi9606.ENSP00000299824.

PTM databases

iPTMnetiQ96T49.
PhosphoSitePlusiQ96T49.
SwissPalmiQ96T49.

Polymorphism and mutation databases

BioMutaiPPP1R16B.
DMDMi22256977.

Proteomic databases

EPDiQ96T49.
MaxQBiQ96T49.
PaxDbiQ96T49.
PeptideAtlasiQ96T49.
PRIDEiQ96T49.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000299824; ENSP00000299824; ENSG00000101445. [Q96T49-1]
ENST00000373331; ENSP00000362428; ENSG00000101445. [Q96T49-2]
GeneIDi26051.
KEGGihsa:26051.
UCSCiuc002xje.3. human. [Q96T49-1]

Organism-specific databases

CTDi26051.
DisGeNETi26051.
GeneCardsiPPP1R16B.
HGNCiHGNC:15850. PPP1R16B.
HPAiHPA049571.
MIMi613275. gene.
neXtProtiNX_Q96T49.
OpenTargetsiENSG00000101445.
PharmGKBiPA33635.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0505. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00760000119141.
HOGENOMiHOG000236276.
HOVERGENiHBG053644.
InParanoidiQ96T49.
KOiK17459.
OMAiNAKDNEL.
OrthoDBiEOG091G06S2.
PhylomeDBiQ96T49.
TreeFamiTF316803.

Miscellaneous databases

ChiTaRSiPPP1R16B. human.
GenomeRNAii26051.
PROiQ96T49.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000101445.
CleanExiHS_PPP1R16B.
GenevisibleiQ96T49. HS.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR017417. Pase-1_reg_su_16AB.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
[Graphical view]
PIRSFiPIRSF038159. PP1_16AB_vert. 1 hit.
SMARTiSM00248. ANK. 5 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPP16B_HUMAN
AccessioniPrimary (citable) accession number: Q96T49
Secondary accession number(s): A2RRR6
, E9PFS8, O94912, Q5W9G4, Q9NQG4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: December 1, 2001
Last modified: November 2, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.