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Protein

Putative RNA-binding protein 15

Gene

RBM15

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May function as an mRNA export factor, stimulating export and expression of RTE-containing mRNAs which are present in many retrotransposons that require to be exported prior to splicing. High affinity binding of pre-mRNA to RBM15 may allow targeting of the mRNP to the export helicase DBP5 in a manner that is independent of splicing-mediated NXF1 deposition, resulting in export prior to splicing. May be implicated in HOX gene regulation.2 Publications

GO - Molecular functioni

  • nucleic acid binding Source: GO_Central
  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000162775-MONOMER.
SIGNORiQ96T37.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative RNA-binding protein 15
Alternative name(s):
One-twenty two protein 1
RNA-binding motif protein 15
Gene namesi
Name:RBM15
Synonyms:OTT, OTT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:14959. RBM15.

Subcellular locationi

GO - Cellular componenti

  • nuclear membrane Source: UniProtKB-SubCell
  • nuclear speck Source: UniProtKB
  • nucleoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nucleus

Pathology & Biotechi

Involvement in diseasei

A chromosomal aberration involving RBM15 may be a cause of acute megakaryoblastic leukemia. Translocation t(1;22)(p13;q13) with MKL1. Although both reciprocal fusion transcripts are detected in acute megakaryoblastic leukemia (AMKL, FAB-M7), the RBM15-MKL1 chimeric protein has all the putative functional domains encoded by each gene and is the candidate oncogene.

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi795K → A: Disrupts interaction with SETD1B. 1 Publication1
Mutagenesisi898K → A: Disrupts interaction with SETD1B. 1 Publication1
Mutagenesisi923F → A: Disrupts interaction with SETD1B. 1 Publication1

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei954 – 955Breakpoint for translocation to form RBM15-MKL12

Keywords - Diseasei

Proto-oncogene

Organism-specific databases

DisGeNETi64783.
MalaCardsiRBM15.
OpenTargetsiENSG00000162775.
Orphaneti402023. 'Megakaryoblastic acute myeloid leukemia with t(1;22)(p13;q13)'.
PharmGKBiPA34264.

Polymorphism and mutation databases

BioMutaiRBM15.
DMDMi32363506.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000817771 – 977Putative RNA-binding protein 15Add BLAST977

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei109PhosphoserineCombined sources1
Modified residuei179PhosphoserineCombined sources1
Modified residuei208PhosphoserineCombined sources1
Modified residuei210PhosphoserineCombined sources1
Modified residuei253PhosphoserineCombined sources1
Modified residuei257PhosphoserineCombined sources1
Modified residuei259PhosphoserineCombined sources1
Modified residuei266PhosphotyrosineCombined sources1
Modified residuei292PhosphoserineCombined sources1
Modified residuei294PhosphoserineCombined sources1
Modified residuei365PhosphoserineCombined sources1
Modified residuei450N6-acetyllysineCombined sources1
Modified residuei568PhosphothreonineCombined sources1
Modified residuei622PhosphoserineCombined sources1
Modified residuei656PhosphoserineCombined sources1
Modified residuei670PhosphoserineCombined sources1
Modified residuei674PhosphoserineCombined sources1
Modified residuei700PhosphoserineCombined sources1
Modified residuei741PhosphoserineCombined sources1
Modified residuei765PhosphoserineCombined sources1
Modified residuei767PhosphoserineCombined sources1
Modified residuei781PhosphoserineCombined sources1
Modified residuei935PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ96T37.
MaxQBiQ96T37.
PaxDbiQ96T37.
PeptideAtlasiQ96T37.
PRIDEiQ96T37.

PTM databases

iPTMnetiQ96T37.
PhosphoSitePlusiQ96T37.
SwissPalmiQ96T37.

Expressioni

Gene expression databases

BgeeiENSG00000162775.
ExpressionAtlasiQ96T37. baseline and differential.
GenevisibleiQ96T37. HS.

Organism-specific databases

HPAiCAB015201.
HPA019824.
HPA049642.

Interactioni

Subunit structurei

Interacts with Epstein-Barr virus BSFL2/BMLF1. Interacts (via SPOC domain) with SETD1B. Interacts with NXF1, the interaction is required to promote mRNA export. Component of the WTAP complex composed of WTAP, ZC3H13, CBLL1, KIAA1429, RBM15, BCLAF1 and THRAP3 (PubMed:24100041).5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CALCOCO2Q131373EBI-2514922,EBI-739580
CEP44Q9C0F13EBI-2514922,EBI-744115
DDX17Q928415EBI-2514922,EBI-746012
LZTS2Q9BRK43EBI-2514922,EBI-741037
NAV2Q8IVL13EBI-2514922,EBI-741200

Protein-protein interaction databases

BioGridi122293. 49 interactors.
IntActiQ96T37. 20 interactors.
MINTiMINT-4131981.
STRINGi9606.ENSP00000358799.

Structurei

3D structure databases

ProteinModelPortaliQ96T37.
SMRiQ96T37.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini170 – 252RRM 1PROSITE-ProRule annotationAdd BLAST83
Domaini374 – 451RRM 2PROSITE-ProRule annotationAdd BLAST78
Domaini455 – 529RRM 3PROSITE-ProRule annotationAdd BLAST75
Domaini777 – 956SPOCPROSITE-ProRule annotationAdd BLAST180

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi60 – 166Gly/Ser-richAdd BLAST107
Compositional biasi616 – 732Arg-richAdd BLAST117

Sequence similaritiesi

Belongs to the RRM Spen family.Curated
Contains 3 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation
Contains 1 SPOC domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0112. Eukaryota.
ENOG410XSAC. LUCA.
GeneTreeiENSGT00530000063730.
HOVERGENiHBG058366.
InParanoidiQ96T37.
KOiK13190.
OMAiVMIIVRV.
OrthoDBiEOG091G0615.
PhylomeDBiQ96T37.
TreeFamiTF315637.

Family and domain databases

Gene3Di2.40.290.10. 1 hit.
3.30.70.330. 3 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR016194. SPOC-like_C_dom.
IPR012921. SPOC_C.
IPR010912. SPOC_met.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
PF07744. SPOC. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF100939. SSF100939. 1 hit.
SSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 3 hits.
PS50917. SPOC. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing and alternative initiation. AlignAdd to basket

Isoform 1 (identifier: Q96T37-1) [UniParc]FASTAAdd to basket
Also known as: RBM15s+ae

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRTAGRDPVP RRSPRWRRAV PLCETSAGRR VTQLRGDDLR RPATMKGKER
60 70 80 90 100
SPVKAKRSRG GEDSTSRGER SKKLGGSGGS NGSSSGKTDS GGGSRRSLHL
110 120 130 140 150
DKSSSRGGSR EYDTGGGSSS SRLHSYSSPS TKNSSGGGES RSSSRGGGGE
160 170 180 190 200
SRSSGAASSA PGGGDGAEYK TLKISELGSQ LSDEAVEDGL FHEFKRFGDV
210 220 230 240 250
SVKISHLSGS GSGDERVAFV NFRRPEDARA AKHARGRLVL YDRPLKIEAV
260 270 280 290 300
YVSRRRSRSP LDKDTYPPSA SVVGASVGGH RHPPGGGGGQ RSLSPGGAAL
310 320 330 340 350
GYRDYRLQQL ALGRLPPPPP PPLPRDLERE RDYPFYERVR PAYSLEPRVG
360 370 380 390 400
AGAGAAPFRE VDEISPEDDQ RANRTLFLGN LDITVTESDL RRAFDRFGVI
410 420 430 440 450
TEVDIKRPSR GQTSTYGFLK FENLDMSHRA KLAMSGKIII RNPIKIGYGK
460 470 480 490 500
ATPTTRLWVG GLGPWVPLAA LAREFDRFGT IRTIDYRKGD SWAYIQYESL
510 520 530 540 550
DAAHAAWTHM RGFPLGGPDR RLRVDFADTE HRYQQQYLQP LPLTHYELVT
560 570 580 590 600
DAFGHRAPDP LRGARDRTPP LLYRDRDRDL YPDSDWVPPP PPVRERSTRT
610 620 630 640 650
AATSVPAYEP LDSLDRRRDG WSLDRDRGDR DLPSSRDQPR KRRLPEESGG
660 670 680 690 700
RHLDRSPESD RPRKRHCAPS PDRSPELSSS RDRYNSDNDR SSRLLLERPS
710 720 730 740 750
PIRDRRGSLE KSQGDKRDRK NSASAERDRK HRTTAPTEGK SPLKKEDRSD
760 770 780 790 800
GSAPSTSTAS SKLKSPSQKQ DGGTAPVASA SPKLCLAWQG MLLLKNSNFP
810 820 830 840 850
SNMHLLQGDL QVASSLLVEG STGGKVAQLK ITQRLRLDQP KLDEVTRRIK
860 870 880 890 900
VAGPNGYAIL LAVPGSSDSR SSSSSAASDT ATSTQRPLRN LVSYLKQKQA
910 920 930 940 950
AGVISLPVGG NKDKENTGVL HAFPPCEFSQ QFLDSPAKAL AKSEEDYLVM
960 970
IIVRGFGFQI GVRYENKKRE NLALTLL
Length:977
Mass (Da):107,189
Last modified:June 27, 2003 - v2
Checksum:iFB26AFE246E40282
GO
Isoform 2 (identifier: Q96T37-2) [UniParc]FASTAAdd to basket
Also known as: RBM15L

The sequence of this isoform differs from the canonical sequence as follows:
     956-977: FGFQIGVRYENKKRENLALTLL → AS

Show »
Length:957
Mass (Da):104,755
Checksum:i72893E069306B4E0
GO
Isoform 3 (identifier: Q96T37-3) [UniParc]FASTAAdd to basket
Also known as: RBM15S

The sequence of this isoform differs from the canonical sequence as follows:
     955-977: GFGFQIGVRYENKKRENLALTLL → AKLVEQRMKIWNSKL

Show »
Length:969
Mass (Da):106,366
Checksum:iE2E52058E76FE38D
GO
Isoform 4 (identifier: Q96T37-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: Missing.
     956-977: FGFQIGVRYENKKRENLALTLL → AS

Note: Produced by alternative initiation of isoform 2.
Show »
Length:913
Mass (Da):99,701
Checksum:i1B4E29CDD062A5AC
GO

Sequence cautioni

The sequence BAB14088 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB15185 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti99H → L in AAK54722 (PubMed:11431691).Curated1
Sequence conflicti99H → L in AAK54723 (PubMed:11431691).Curated1
Sequence conflicti99H → L in AAK54724 (PubMed:11431691).Curated1
Sequence conflicti99H → L in BAB14088 (Ref. 3) Curated1
Sequence conflicti227D → N in AAI03494 (PubMed:15489334).Curated1
Sequence conflicti705R → G in AAK54722 (PubMed:11431691).Curated1
Sequence conflicti705R → G in AAK54723 (PubMed:11431691).Curated1
Sequence conflicti705R → G in AAK54724 (PubMed:11431691).Curated1
Sequence conflicti705R → G in BAB14088 (Ref. 3) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0538771 – 44Missing in isoform 4. CuratedAdd BLAST44
Alternative sequenceiVSP_005812955 – 977GFGFQ…ALTLL → AKLVEQRMKIWNSKL in isoform 3. 2 PublicationsAdd BLAST23
Alternative sequenceiVSP_005811956 – 977FGFQI…ALTLL → AS in isoform 2 and isoform 4. 1 PublicationAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF368062 mRNA. Translation: AAK54722.1.
AF368063 mRNA. Translation: AAK54723.1.
AF368064 mRNA. Translation: AAK54724.1.
AF364035 mRNA. Translation: AAK56920.1. Different termination.
AJ303089 mRNA. Translation: CAC38828.1. Different termination.
AJ303090 mRNA. Translation: CAC38829.1. Different termination.
AJ297259 Genomic DNA. Translation: CAC38861.1.
AJ297259 Genomic DNA. Translation: CAC38862.1.
AK022541 mRNA. Translation: BAB14088.1. Different initiation.
AK025596 mRNA. Translation: BAB15185.1. Different initiation.
AL355488 Genomic DNA. Translation: CAI19077.1.
CH471122 Genomic DNA. Translation: EAW56439.1.
BC006397 mRNA. Translation: AAH06397.2.
BC047479 mRNA. Translation: AAH47479.1.
BC062316 mRNA. Translation: AAH62316.1.
BC098140 mRNA. Translation: AAH98140.1.
BC103493 mRNA. Translation: AAI03494.1.
BC103507 mRNA. Translation: AAI03508.1.
BK005915 mRNA. Translation: DAA05818.1.
CCDSiCCDS59198.1. [Q96T37-3]
CCDS822.1. [Q96T37-1]
RefSeqiNP_001188474.1. NM_001201545.1. [Q96T37-3]
NP_073605.4. NM_022768.4. [Q96T37-1]
UniGeneiHs.435947.
Hs.708172.
Hs.732334.

Genome annotation databases

EnsembliENST00000369784; ENSP00000358799; ENSG00000162775. [Q96T37-1]
ENST00000487146; ENSP00000473552; ENSG00000162775. [Q96T37-3]
ENST00000602849; ENSP00000473638; ENSG00000162775. [Q96T37-2]
ENST00000618772; ENSP00000483133; ENSG00000162775. [Q96T37-1]
GeneIDi64783.
KEGGihsa:64783.
UCSCiuc001dzl.1. human. [Q96T37-1]

Keywords - Coding sequence diversityi

Alternative initiation, Alternative splicing, Chromosomal rearrangement

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF368062 mRNA. Translation: AAK54722.1.
AF368063 mRNA. Translation: AAK54723.1.
AF368064 mRNA. Translation: AAK54724.1.
AF364035 mRNA. Translation: AAK56920.1. Different termination.
AJ303089 mRNA. Translation: CAC38828.1. Different termination.
AJ303090 mRNA. Translation: CAC38829.1. Different termination.
AJ297259 Genomic DNA. Translation: CAC38861.1.
AJ297259 Genomic DNA. Translation: CAC38862.1.
AK022541 mRNA. Translation: BAB14088.1. Different initiation.
AK025596 mRNA. Translation: BAB15185.1. Different initiation.
AL355488 Genomic DNA. Translation: CAI19077.1.
CH471122 Genomic DNA. Translation: EAW56439.1.
BC006397 mRNA. Translation: AAH06397.2.
BC047479 mRNA. Translation: AAH47479.1.
BC062316 mRNA. Translation: AAH62316.1.
BC098140 mRNA. Translation: AAH98140.1.
BC103493 mRNA. Translation: AAI03494.1.
BC103507 mRNA. Translation: AAI03508.1.
BK005915 mRNA. Translation: DAA05818.1.
CCDSiCCDS59198.1. [Q96T37-3]
CCDS822.1. [Q96T37-1]
RefSeqiNP_001188474.1. NM_001201545.1. [Q96T37-3]
NP_073605.4. NM_022768.4. [Q96T37-1]
UniGeneiHs.435947.
Hs.708172.
Hs.732334.

3D structure databases

ProteinModelPortaliQ96T37.
SMRiQ96T37.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122293. 49 interactors.
IntActiQ96T37. 20 interactors.
MINTiMINT-4131981.
STRINGi9606.ENSP00000358799.

PTM databases

iPTMnetiQ96T37.
PhosphoSitePlusiQ96T37.
SwissPalmiQ96T37.

Polymorphism and mutation databases

BioMutaiRBM15.
DMDMi32363506.

Proteomic databases

EPDiQ96T37.
MaxQBiQ96T37.
PaxDbiQ96T37.
PeptideAtlasiQ96T37.
PRIDEiQ96T37.

Protocols and materials databases

DNASUi64783.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000369784; ENSP00000358799; ENSG00000162775. [Q96T37-1]
ENST00000487146; ENSP00000473552; ENSG00000162775. [Q96T37-3]
ENST00000602849; ENSP00000473638; ENSG00000162775. [Q96T37-2]
ENST00000618772; ENSP00000483133; ENSG00000162775. [Q96T37-1]
GeneIDi64783.
KEGGihsa:64783.
UCSCiuc001dzl.1. human. [Q96T37-1]

Organism-specific databases

CTDi64783.
DisGeNETi64783.
GeneCardsiRBM15.
HGNCiHGNC:14959. RBM15.
HPAiCAB015201.
HPA019824.
HPA049642.
MalaCardsiRBM15.
MIMi606077. gene.
neXtProtiNX_Q96T37.
OpenTargetsiENSG00000162775.
Orphaneti402023. 'Megakaryoblastic acute myeloid leukemia with t(1;22)(p13;q13)'.
PharmGKBiPA34264.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0112. Eukaryota.
ENOG410XSAC. LUCA.
GeneTreeiENSGT00530000063730.
HOVERGENiHBG058366.
InParanoidiQ96T37.
KOiK13190.
OMAiVMIIVRV.
OrthoDBiEOG091G0615.
PhylomeDBiQ96T37.
TreeFamiTF315637.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000162775-MONOMER.
SIGNORiQ96T37.

Miscellaneous databases

ChiTaRSiRBM15. human.
GeneWikiiRBM15.
GenomeRNAii64783.
PROiQ96T37.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000162775.
ExpressionAtlasiQ96T37. baseline and differential.
GenevisibleiQ96T37. HS.

Family and domain databases

Gene3Di2.40.290.10. 1 hit.
3.30.70.330. 3 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR016194. SPOC-like_C_dom.
IPR012921. SPOC_C.
IPR010912. SPOC_met.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
PF07744. SPOC. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF100939. SSF100939. 1 hit.
SSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 3 hits.
PS50917. SPOC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRBM15_HUMAN
AccessioniPrimary (citable) accession number: Q96T37
Secondary accession number(s): A1A693
, Q3ZB86, Q4V760, Q5D058, Q5T613, Q86VW9, Q96PE4, Q96SC5, Q96SC6, Q96SC9, Q96SD0, Q96T38, Q9BRA5, Q9H6R8, Q9H9Y0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: June 27, 2003
Last modified: November 30, 2016
This is version 160 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.