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Q96SZ6 (CK5P1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
CDK5 regulatory subunit-associated protein 1
Alternative name(s):
CDK5 activator-binding protein C42
Gene names
Name:CDK5RAP1
Synonyms:C20orf34
ORF Names:CGI-05, HSPC167
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length601 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Specifically inhibits CDK5 activation by CDK5R1. HAMAP-Rule MF_01864

Cofactor

Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Subunit structure

Interacts with CDK5R1 (p35 form). CDK5RAP1, CDK5RAP2 and CDK5RAP3 show competitive binding to CDK5R1. Forms a complex with CDK5R1 and CDK5. Ref.9

Tissue specificity

Widely expressed. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Ref.8

Sequence similarities

Belongs to the methylthiotransferase family. MiaB subfamily.

Contains 1 MTTase N-terminal domain.

Contains 1 TRAM domain.

Sequence caution

The sequence AAD34147.1 differs from that shown. Reason: Frameshift at positions 3, 32, 128, 133 and 569.

The sequence AAF29131.1 differs from that shown. Reason: Frameshift at positions 213, 244, 267 and 282.

The sequence BAB55120.1 differs from that shown. Reason: Erroneous termination at position 500. Translated as Glu.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

EXOSC3Q9NQT51EBI-372956,EBI-371866
EXOSC5Q9NQT41EBI-372956,EBI-371876

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96SZ6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: May be due to intron retention. No experimental confirmation available.
Isoform 2 (identifier: Q96SZ6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-90: Missing.
     293-306: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q96SZ6-3)

Also known as: a;

The sequence of this isoform differs from the canonical sequence as follows:
     293-306: Missing.
Isoform 4 (identifier: Q96SZ6-4)

The sequence of this isoform differs from the canonical sequence as follows:
     293-383: Missing.
Note: No experimental confirmation available.
Isoform 5 (identifier: Q96SZ6-5)

Also known as: CDK5RAP1_v4;

The sequence of this isoform differs from the canonical sequence as follows:
     292-305: Missing.
     435-440: GVSLSS → EDTGIS
     441-601: Missing.
Note: Mainly expressed in brain, placenta and testis.
Isoform 6 (identifier: Q96SZ6-6)

Also known as: CDK5RAP1_v3;

The sequence of this isoform differs from the canonical sequence as follows:
     292-305: Missing.
     576-601: ITSASSQTLRGHVLCRTTLRDSSAYC → VRSPFGLLACTVK
Note: High expression in placenta and lung.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 601601CDK5 regulatory subunit-associated protein 1 HAMAP-Rule MF_01864
PRO_0000141764

Regions

Domain100 – 220121MTTase N-terminal
Domain515 – 59076TRAM
Region22 – 157136CDK5 activation inhibition HAMAP-Rule MF_01864
Region475 – 586112CDK5R1-binding HAMAP-Rule MF_01864

Sites

Metal binding1091Iron-sulfur (4Fe-4S) By similarity
Metal binding1451Iron-sulfur (4Fe-4S) By similarity
Metal binding1831Iron-sulfur (4Fe-4S) By similarity
Metal binding2581Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding2621Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding2651Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Natural variations

Alternative sequence1 – 9090Missing in isoform 2.
VSP_007560
Alternative sequence292 – 30514Missing in isoform 5 and isoform 6.
VSP_047799
Alternative sequence293 – 38391Missing in isoform 4.
VSP_007561
Alternative sequence293 – 30614Missing in isoform 2 and isoform 3.
VSP_007562
Alternative sequence435 – 4406GVSLSS → EDTGIS in isoform 5.
VSP_047800
Alternative sequence441 – 601161Missing in isoform 5.
VSP_047801
Alternative sequence576 – 60126ITSAS…SSAYC → VRSPFGLLACTVK in isoform 6.
VSP_047802

Experimental info

Sequence conflict2131R → Q in AAH01215. Ref.7
Sequence conflict2171V → D Ref.2
Sequence conflict2691F → L in BAB55120. Ref.4
Sequence conflict2821I → T in BAB55120. Ref.4
Sequence conflict3231D → G in BAB55120. Ref.4
Sequence conflict4641V → A in BAB14760. Ref.4
Sequence conflict5111T → S in AAF29131. Ref.2
Sequence conflict5791Missing Ref.1
Sequence conflict5851R → K in BAB14760. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 16, 2003. Version 2.
Checksum: 4AEBFD7DA5400902

FASTA60167,689
        10         20         30         40         50         60 
MHPLQCVLQV QRSLGWGPLA SVSWLSLRMC RAHSSLSSTM CPSPERQEDG ARKDFSSRLA 

        70         80         90        100        110        120 
AGPTFQHFLK SASAPQEKLS SEVEDPPPYL MMDELLGRQR KVYLETYGCQ MNVNDTEIAW 

       130        140        150        160        170        180 
SILQKSGYLR TSNLQEADVI LLVTCSIREK AEQTIWNRLH QLKALKTRRP RSRVPLRIGI 

       190        200        210        220        230        240 
LGCMAERLKE EILNREKMVD ILAGPDAYRD LPRLLAVAES GQQAANVLLS LDETYADVMP 

       250        260        270        280        290        300 
VQTSASATSA FVSIMRGCDN MCSYCIVPFT RGRERSRPIA SILEEVKKLS EQVFLPPRPP 

       310        320        330        340        350        360 
KVLGLQGLKE VTLLGQNVNS FRDNSEVQFN SAVPTNLSRG FTTNYKTKQG GLRFAHLLDQ 

       370        380        390        400        410        420 
VSRVDPEMRI RFTSPHPKDF PDEVLQLIHE RDNICKQIHL PAQSGSSRVL EAMRRGYSRE 

       430        440        450        460        470        480 
AYVELVHHIR ESIPGVSLSS DFIAGFCGET EEDHVQTVSL LREVQYNMGF LFAYSMRQKT 

       490        500        510        520        530        540 
RAYHRLKDDV PEEVKLRRLE ELITIFREEA TKANQTSVGC TQLVLVEGLS KRSATDLCGR 

       550        560        570        580        590        600 
NDGNLKVIFP DAEMEDVNNP GLRVRAQPGD YVLVKITSAS SQTLRGHVLC RTTLRDSSAY 


C 

« Hide

Isoform 2 [UniParc].

Checksum: C8FFBA14A5C3EB09
Show »

FASTA49756,223
Isoform 3 (a) [UniParc].

Checksum: 314E826B35C346EB
Show »

FASTA58766,146
Isoform 4 [UniParc].

Checksum: 8F0DFB3B71E135BB
Show »

FASTA51057,413
Isoform 5 (CDK5RAP1_v4) [UniParc].

Checksum: D74ADCD179EA4FDC
Show »

FASTA42648,072
Isoform 6 (CDK5RAP1_v3) [UniParc].

Checksum: A542DA7E2496A56B
Show »

FASTA57464,709

References

« Hide 'large scale' references
[1]"Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics."
Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.
Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[2]"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. expand/collapse author list , Gu J., Chen S.-J., Chen Z.
Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Umbilical cord blood.
[3]"Cloning, characterization and expression of CDK5RAP1_v3 and CDK5RAP1_v4, two novel splice variants of human CDK5RAP1."
Zou X., Ji C., Jin F., Liu J., Wu M., Zheng H., Wang Y., Li X., Xu J., Gu S., Xie Y., Mao Y.
Genes Genet. Syst. 79:177-182(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 5 AND 6).
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
Tissue: Retinoblastoma, Stomach and Teratocarcinoma.
[5]"The DNA sequence and comparative analysis of human chromosome 20."
Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. expand/collapse author list , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
Tissue: Duodenum and Lung.
[8]"Cloning of three novel neuronal Cdk5 activator binding proteins."
Ching Y.-P., Qi Z., Wang J.H.
Gene 242:285-294(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[9]"Identification of a neuronal Cdk5 activator-binding protein as Cdk5 inhibitor."
Ching Y.-P., Pang A.S.H., Lam W.-H., Qi R.Z., Wang J.H.
J. Biol. Chem. 277:15237-15240(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CDK5 AND CDK5R1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF152097 mRNA. Translation: AAD34147.1. Frameshift.
AF161516 mRNA. Translation: AAF29131.1. Frameshift.
AY462283 mRNA. Translation: AAS18317.1.
AY462284 mRNA. Translation: AAS18318.1.
AK023992 mRNA. Translation: BAB14760.1.
AK027449 mRNA. Translation: BAB55120.1. Sequence problems.
AK292075 mRNA. Translation: BAF84764.1.
AL355392 Genomic DNA. Translation: CAI19392.1.
AL355392 Genomic DNA. Translation: CAI19393.1.
AL355392 Genomic DNA. Translation: CAI19394.1.
CH471077 Genomic DNA. Translation: EAW76319.1.
CH471077 Genomic DNA. Translation: EAW76322.1.
CH471077 Genomic DNA. Translation: EAW76323.1.
BC001215 mRNA. Translation: AAH01215.1.
BC050706 mRNA. Translation: AAH50706.1.
CCDSCCDS13219.1. [Q96SZ6-3]
CCDS63255.1. [Q96SZ6-4]
RefSeqNP_001265097.1. NM_001278168.1. [Q96SZ6-4]
NP_001265098.1. NM_001278169.1.
NP_057166.4. NM_016082.4.
NP_057492.2. NM_016408.3. [Q96SZ6-3]
UniGeneHs.435952.

3D structure databases

ProteinModelPortalQ96SZ6.
SMRQ96SZ6. Positions 272-528.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119661. 6 interactions.
IntActQ96SZ6. 2 interactions.

PTM databases

PhosphoSiteQ96SZ6.

Polymorphism databases

DMDM32129446.

Proteomic databases

MaxQBQ96SZ6.
PaxDbQ96SZ6.
PRIDEQ96SZ6.

Protocols and materials databases

DNASU51654.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000339269; ENSP00000341840; ENSG00000101391. [Q96SZ6-4]
ENST00000346416; ENSP00000217372; ENSG00000101391. [Q96SZ6-3]
ENST00000357886; ENSP00000350558; ENSG00000101391. [Q96SZ6-1]
ENST00000473997; ENSP00000476857; ENSG00000101391. [Q96SZ6-2]
ENST00000544843; ENSP00000439034; ENSG00000101391. [Q96SZ6-5]
GeneID51654.
KEGGhsa:51654.
UCSCuc002wyy.4. human. [Q96SZ6-3]
uc010gek.4. human. [Q96SZ6-1]
uc010gem.4. human. [Q96SZ6-4]

Organism-specific databases

CTD51654.
GeneCardsGC20M031946.
HGNCHGNC:15880. CDK5RAP1.
MIM608200. gene.
neXtProtNX_Q96SZ6.
PharmGKBPA26313.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0621.
HOVERGENHBG050975.
InParanoidQ96SZ6.
OMAIEVYKDT.
OrthoDBEOG744T8N.
PhylomeDBQ96SZ6.
TreeFamTF101033.

Gene expression databases

ArrayExpressQ96SZ6.
BgeeQ96SZ6.
CleanExHS_CDK5RAP1.
GenevestigatorQ96SZ6.

Family and domain databases

Gene3D3.80.30.20. 1 hit.
HAMAPMF_01864. tRNA_metthiotr_MiaB.
InterProIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERPTHR11918. PTHR11918. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00089. TIGR00089. 1 hit.
PROSITEPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCDK5RAP1. human.
GeneWikiCDK5RAP1.
GenomeRNAi51654.
NextBio55620.
PROQ96SZ6.
SOURCESearch...

Entry information

Entry nameCK5P1_HUMAN
AccessionPrimary (citable) accession number: Q96SZ6
Secondary accession number(s): A8K7R0 expand/collapse secondary AC list , Q5QP46, Q5QP47, Q5QP48, Q675N4, Q675N5, Q9BVG6, Q9BWZ5, Q9H859, Q9NZZ9, Q9Y3F0
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: June 16, 2003
Last modified: July 9, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 20

Human chromosome 20: entries, gene names and cross-references to MIM