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Protein

CDK5 regulatory subunit-associated protein 1

Gene

CDK5RAP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specifically inhibits CDK5 activation by CDK5R1.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi109Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi145Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi183Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi258Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi262Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi265Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
CDK5 regulatory subunit-associated protein 1
Alternative name(s):
CDK5 activator-binding protein C42
Gene namesi
Name:CDK5RAP1
Synonyms:C20orf34
ORF Names:CGI-05, HSPC167
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

HGNCiHGNC:15880. CDK5RAP1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi51654.
OpenTargetsiENSG00000101391.
PharmGKBiPA26313.

Polymorphism and mutation databases

DMDMi32129446.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001417641 – 601CDK5 regulatory subunit-associated protein 1Add BLAST601

Proteomic databases

EPDiQ96SZ6.
MaxQBiQ96SZ6.
PaxDbiQ96SZ6.
PeptideAtlasiQ96SZ6.
PRIDEiQ96SZ6.

PTM databases

iPTMnetiQ96SZ6.
PhosphoSitePlusiQ96SZ6.

Expressioni

Tissue specificityi

Widely expressed. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.1 Publication

Gene expression databases

BgeeiENSG00000101391.
CleanExiHS_CDK5RAP1.
ExpressionAtlasiQ96SZ6. baseline and differential.
GenevisibleiQ96SZ6. HS.

Interactioni

Subunit structurei

Interacts with CDK5R1 (p35 form). CDK5RAP1, CDK5RAP2 and CDK5RAP3 show competitive binding to CDK5R1. Forms a complex with CDK5R1 and CDK5.1 Publication

GO - Molecular functioni

  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi119661. 17 interactors.
IntActiQ96SZ6. 3 interactors.
STRINGi9606.ENSP00000217372.

Structurei

3D structure databases

ProteinModelPortaliQ96SZ6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini100 – 220MTTase N-terminalAdd BLAST121
Domaini515 – 590TRAMAdd BLAST76

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni22 – 157CDK5 activation inhibitionAdd BLAST136
Regioni475 – 586CDK5R1-bindingAdd BLAST112

Sequence similaritiesi

Contains 1 MTTase N-terminal domain.Curated
Contains 1 TRAM domain.Curated

Phylogenomic databases

eggNOGiKOG2492. Eukaryota.
COG0621. LUCA.
GeneTreeiENSGT00530000063447.
HOVERGENiHBG050975.
InParanoidiQ96SZ6.
OMAiCQMNFSD.
OrthoDBiEOG091G07M5.
PhylomeDBiQ96SZ6.
TreeFamiTF101033.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB. 1 hit.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 2 hits.
PfamiPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96SZ6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHPLQCVLQV QRSLGWGPLA SVSWLSLRMC RAHSSLSSTM CPSPERQEDG
60 70 80 90 100
ARKDFSSRLA AGPTFQHFLK SASAPQEKLS SEVEDPPPYL MMDELLGRQR
110 120 130 140 150
KVYLETYGCQ MNVNDTEIAW SILQKSGYLR TSNLQEADVI LLVTCSIREK
160 170 180 190 200
AEQTIWNRLH QLKALKTRRP RSRVPLRIGI LGCMAERLKE EILNREKMVD
210 220 230 240 250
ILAGPDAYRD LPRLLAVAES GQQAANVLLS LDETYADVMP VQTSASATSA
260 270 280 290 300
FVSIMRGCDN MCSYCIVPFT RGRERSRPIA SILEEVKKLS EQVFLPPRPP
310 320 330 340 350
KVLGLQGLKE VTLLGQNVNS FRDNSEVQFN SAVPTNLSRG FTTNYKTKQG
360 370 380 390 400
GLRFAHLLDQ VSRVDPEMRI RFTSPHPKDF PDEVLQLIHE RDNICKQIHL
410 420 430 440 450
PAQSGSSRVL EAMRRGYSRE AYVELVHHIR ESIPGVSLSS DFIAGFCGET
460 470 480 490 500
EEDHVQTVSL LREVQYNMGF LFAYSMRQKT RAYHRLKDDV PEEVKLRRLE
510 520 530 540 550
ELITIFREEA TKANQTSVGC TQLVLVEGLS KRSATDLCGR NDGNLKVIFP
560 570 580 590 600
DAEMEDVNNP GLRVRAQPGD YVLVKITSAS SQTLRGHVLC RTTLRDSSAY

C
Note: May be due to intron retention. No experimental confirmation available.
Length:601
Mass (Da):67,689
Last modified:June 16, 2003 - v2
Checksum:i4AEBFD7DA5400902
GO
Isoform 2 (identifier: Q96SZ6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-90: Missing.
     293-306: Missing.

Note: No experimental confirmation available.
Show »
Length:497
Mass (Da):56,223
Checksum:iC8FFBA14A5C3EB09
GO
Isoform 3 (identifier: Q96SZ6-3) [UniParc]FASTAAdd to basket
Also known as: a

The sequence of this isoform differs from the canonical sequence as follows:
     293-306: Missing.

Show »
Length:587
Mass (Da):66,146
Checksum:i314E826B35C346EB
GO
Isoform 4 (identifier: Q96SZ6-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     293-383: Missing.

Note: No experimental confirmation available.
Show »
Length:510
Mass (Da):57,413
Checksum:i8F0DFB3B71E135BB
GO
Isoform 5 (identifier: Q96SZ6-5) [UniParc]FASTAAdd to basket
Also known as: CDK5RAP1_v4

The sequence of this isoform differs from the canonical sequence as follows:
     292-305: Missing.
     435-440: GVSLSS → EDTGIS
     441-601: Missing.

Note: Mainly expressed in brain, placenta and testis.
Show »
Length:426
Mass (Da):48,072
Checksum:iD74ADCD179EA4FDC
GO
Isoform 6 (identifier: Q96SZ6-6) [UniParc]FASTAAdd to basket
Also known as: CDK5RAP1_v3

The sequence of this isoform differs from the canonical sequence as follows:
     292-305: Missing.
     576-601: ITSASSQTLRGHVLCRTTLRDSSAYC → VRSPFGLLACTVK

Note: High expression in placenta and lung.
Show »
Length:574
Mass (Da):64,709
Checksum:iA542DA7E2496A56B
GO

Sequence cautioni

The sequence AAD34147 differs from that shown. Reason: Frameshift at positions 3, 32, 128, 133 and 569.Curated
The sequence AAF29131 differs from that shown. Reason: Frameshift at positions 213, 244, 267 and 282.Curated
The sequence BAB55120 differs from that shown. Reason: Erroneous termination at position 500. Translated as Glu.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti213R → Q in AAH01215 (PubMed:15489334).Curated1
Sequence conflicti217V → D (PubMed:11042152).Curated1
Sequence conflicti269F → L in BAB55120 (PubMed:14702039).Curated1
Sequence conflicti282I → T in BAB55120 (PubMed:14702039).Curated1
Sequence conflicti323D → G in BAB55120 (PubMed:14702039).Curated1
Sequence conflicti464V → A in BAB14760 (PubMed:14702039).Curated1
Sequence conflicti511T → S in AAF29131 (PubMed:11042152).Curated1
Sequence conflicti579Missing (PubMed:10810093).Curated1
Sequence conflicti585R → K in BAB14760 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0075601 – 90Missing in isoform 2. 1 PublicationAdd BLAST90
Alternative sequenceiVSP_047799292 – 305Missing in isoform 5 and isoform 6. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_007561293 – 383Missing in isoform 4. 1 PublicationAdd BLAST91
Alternative sequenceiVSP_007562293 – 306Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST14
Alternative sequenceiVSP_047800435 – 440GVSLSS → EDTGIS in isoform 5. 1 Publication6
Alternative sequenceiVSP_047801441 – 601Missing in isoform 5. 1 PublicationAdd BLAST161
Alternative sequenceiVSP_047802576 – 601ITSAS…SSAYC → VRSPFGLLACTVK in isoform 6. 1 PublicationAdd BLAST26

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF152097 mRNA. Translation: AAD34147.1. Frameshift.
AF161516 mRNA. Translation: AAF29131.1. Frameshift.
AY462283 mRNA. Translation: AAS18317.1.
AY462284 mRNA. Translation: AAS18318.1.
AK023992 mRNA. Translation: BAB14760.1.
AK027449 mRNA. Translation: BAB55120.1. Sequence problems.
AK292075 mRNA. Translation: BAF84764.1.
AL355392 Genomic DNA. Translation: CAI19392.1.
AL355392 Genomic DNA. Translation: CAI19393.1.
AL355392 Genomic DNA. Translation: CAI19394.1.
CH471077 Genomic DNA. Translation: EAW76319.1.
CH471077 Genomic DNA. Translation: EAW76322.1.
CH471077 Genomic DNA. Translation: EAW76323.1.
BC001215 mRNA. Translation: AAH01215.1.
BC050706 mRNA. Translation: AAH50706.1.
CCDSiCCDS13219.1. [Q96SZ6-3]
CCDS63255.1. [Q96SZ6-4]
RefSeqiNP_001265097.1. NM_001278168.1. [Q96SZ6-4]
NP_001265098.1. NM_001278169.1.
NP_057166.4. NM_016082.4.
NP_057492.2. NM_016408.3. [Q96SZ6-3]
UniGeneiHs.435952.

Genome annotation databases

EnsembliENST00000339269; ENSP00000341840; ENSG00000101391. [Q96SZ6-4]
ENST00000346416; ENSP00000217372; ENSG00000101391. [Q96SZ6-3]
ENST00000357886; ENSP00000350558; ENSG00000101391. [Q96SZ6-1]
ENST00000473997; ENSP00000476857; ENSG00000101391. [Q96SZ6-2]
GeneIDi51654.
KEGGihsa:51654.
UCSCiuc002wyz.5. human. [Q96SZ6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF152097 mRNA. Translation: AAD34147.1. Frameshift.
AF161516 mRNA. Translation: AAF29131.1. Frameshift.
AY462283 mRNA. Translation: AAS18317.1.
AY462284 mRNA. Translation: AAS18318.1.
AK023992 mRNA. Translation: BAB14760.1.
AK027449 mRNA. Translation: BAB55120.1. Sequence problems.
AK292075 mRNA. Translation: BAF84764.1.
AL355392 Genomic DNA. Translation: CAI19392.1.
AL355392 Genomic DNA. Translation: CAI19393.1.
AL355392 Genomic DNA. Translation: CAI19394.1.
CH471077 Genomic DNA. Translation: EAW76319.1.
CH471077 Genomic DNA. Translation: EAW76322.1.
CH471077 Genomic DNA. Translation: EAW76323.1.
BC001215 mRNA. Translation: AAH01215.1.
BC050706 mRNA. Translation: AAH50706.1.
CCDSiCCDS13219.1. [Q96SZ6-3]
CCDS63255.1. [Q96SZ6-4]
RefSeqiNP_001265097.1. NM_001278168.1. [Q96SZ6-4]
NP_001265098.1. NM_001278169.1.
NP_057166.4. NM_016082.4.
NP_057492.2. NM_016408.3. [Q96SZ6-3]
UniGeneiHs.435952.

3D structure databases

ProteinModelPortaliQ96SZ6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119661. 17 interactors.
IntActiQ96SZ6. 3 interactors.
STRINGi9606.ENSP00000217372.

PTM databases

iPTMnetiQ96SZ6.
PhosphoSitePlusiQ96SZ6.

Polymorphism and mutation databases

DMDMi32129446.

Proteomic databases

EPDiQ96SZ6.
MaxQBiQ96SZ6.
PaxDbiQ96SZ6.
PeptideAtlasiQ96SZ6.
PRIDEiQ96SZ6.

Protocols and materials databases

DNASUi51654.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000339269; ENSP00000341840; ENSG00000101391. [Q96SZ6-4]
ENST00000346416; ENSP00000217372; ENSG00000101391. [Q96SZ6-3]
ENST00000357886; ENSP00000350558; ENSG00000101391. [Q96SZ6-1]
ENST00000473997; ENSP00000476857; ENSG00000101391. [Q96SZ6-2]
GeneIDi51654.
KEGGihsa:51654.
UCSCiuc002wyz.5. human. [Q96SZ6-1]

Organism-specific databases

CTDi51654.
DisGeNETi51654.
GeneCardsiCDK5RAP1.
HGNCiHGNC:15880. CDK5RAP1.
MIMi608200. gene.
neXtProtiNX_Q96SZ6.
OpenTargetsiENSG00000101391.
PharmGKBiPA26313.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2492. Eukaryota.
COG0621. LUCA.
GeneTreeiENSGT00530000063447.
HOVERGENiHBG050975.
InParanoidiQ96SZ6.
OMAiCQMNFSD.
OrthoDBiEOG091G07M5.
PhylomeDBiQ96SZ6.
TreeFamiTF101033.

Miscellaneous databases

ChiTaRSiCDK5RAP1. human.
GeneWikiiCDK5RAP1.
GenomeRNAii51654.
PROiQ96SZ6.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000101391.
CleanExiHS_CDK5RAP1.
ExpressionAtlasiQ96SZ6. baseline and differential.
GenevisibleiQ96SZ6. HS.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB. 1 hit.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 2 hits.
PfamiPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCK5P1_HUMAN
AccessioniPrimary (citable) accession number: Q96SZ6
Secondary accession number(s): A8K7R0
, Q5QP46, Q5QP47, Q5QP48, Q675N4, Q675N5, Q9BVG6, Q9BWZ5, Q9H859, Q9NZZ9, Q9Y3F0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: June 16, 2003
Last modified: November 30, 2016
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.