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Protein

Protein IWS1 homolog

Gene

IWS1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor which plays a key role in defining the composition of the RNA polymerase II (RNAPII) elongation complex and in modulating the production of mature mRNA transcripts. Acts as an assembly factor to recruit various factors to the RNAPII elongation complex and is recruited to the complex via binding to the transcription elongation factor SUPT6H bound to the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2) to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription.3 Publications

GO - Molecular functioni

GO - Biological processi

  • mRNA processing Source: UniProtKB-KW
  • mRNA transport Source: UniProtKB-KW
  • regulation of DNA-templated transcription, elongation Source: GO_Central
  • regulation of histone H3-K36 trimethylation Source: UniProtKB
  • regulation of histone H4 acetylation Source: UniProtKB
  • regulation of mRNA export from nucleus Source: UniProtKB
  • regulation of mRNA processing Source: UniProtKB
  • RNA splicing Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing, mRNA transport, Transcription, Transcription regulation, Transport

Enzyme and pathway databases

ReactomeiR-HSA-112382. Formation of RNA Pol II elongation complex.
R-HSA-112387. Elongation arrest and recovery.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-75955. RNA Polymerase II Transcription Elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein IWS1 homolog
Alternative name(s):
IWS1-like protein
Gene namesi
Name:IWS1
Synonyms:IWS1L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:25467. IWS1.

Subcellular locationi

GO - Cellular componenti

  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000163166.
PharmGKBiPA144596419.

Polymorphism and mutation databases

BioMutaiIWS1.
DMDMi85542184.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000833461 – 819Protein IWS1 homologAdd BLAST819

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei27PhosphoserineBy similarity1
Modified residuei54PhosphoserineCombined sources1
Modified residuei69PhosphoserineCombined sources1
Modified residuei157PhosphoserineCombined sources1
Modified residuei159PhosphoserineCombined sources1
Modified residuei183PhosphoserineCombined sources1
Modified residuei196PhosphoserineCombined sources1
Modified residuei198PhosphoserineCombined sources1
Modified residuei209PhosphoserineBy similarity1
Modified residuei235PhosphoserineCombined sources1
Modified residuei237PhosphoserineCombined sources1
Modified residuei248PhosphoserineCombined sources1
Modified residuei250PhosphoserineCombined sources1
Modified residuei261PhosphoserineCombined sources1
Modified residuei263PhosphoserineCombined sources1
Modified residuei274PhosphoserineCombined sources1
Modified residuei276PhosphoserineCombined sources1
Modified residuei287PhosphoserineCombined sources1
Modified residuei289PhosphoserineCombined sources1
Modified residuei300PhosphoserineCombined sources1
Modified residuei302PhosphoserineCombined sources1
Modified residuei304PhosphoserineCombined sources1
Modified residuei313PhosphoserineCombined sources1
Modified residuei315PhosphoserineCombined sources1
Modified residuei329PhosphoserineCombined sources1
Modified residuei333PhosphoserineCombined sources1
Modified residuei351PhosphoserineBy similarity1
Modified residuei362PhosphoserineBy similarity1
Modified residuei363PhosphoserineCombined sources1
Modified residuei365PhosphoserineCombined sources1
Modified residuei377PhosphoserineCombined sources1
Modified residuei398PhosphoserineCombined sources1
Modified residuei400PhosphoserineCombined sources1
Modified residuei415PhosphoserineCombined sources1
Modified residuei420PhosphoserineCombined sources1
Modified residuei422PhosphoserineCombined sources1
Modified residuei426PhosphoserineBy similarity1
Modified residuei435PhosphothreonineCombined sources1
Modified residuei438PhosphoserineCombined sources1
Modified residuei440PhosphoserineCombined sources1
Modified residuei480PhosphoserineCombined sources1
Modified residuei511PhosphoserineCombined sources1
Modified residuei513PhosphoserineCombined sources1
Modified residuei725PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ96ST2.
MaxQBiQ96ST2.
PaxDbiQ96ST2.
PeptideAtlasiQ96ST2.
PRIDEiQ96ST2.

PTM databases

iPTMnetiQ96ST2.
PhosphoSitePlusiQ96ST2.

Expressioni

Gene expression databases

BgeeiENSG00000163166.
CleanExiHS_IWS1.
ExpressionAtlasiQ96ST2. baseline and differential.
GenevisibleiQ96ST2. HS.

Organism-specific databases

HPAiHPA035719.
HPA061866.

Interactioni

Subunit structurei

Interacts with SUPT6H; binds preferentially to the POLR2A-bound SUPT6H. Interacts with ALYREF/THOC4, SETD2 and PRMT5. Interacts with HDGFRP2.4 Publications

Protein-protein interaction databases

BioGridi120807. 33 interactors.
IntActiQ96ST2. 22 interactors.
STRINGi9606.ENSP00000295321.

Structurei

3D structure databases

ProteinModelPortaliQ96ST2.
SMRiQ96ST2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati235 – 2601Add BLAST26
Repeati261 – 2862Add BLAST26
Repeati287 – 2963; half-length10
Domaini614 – 692TFIIS N-terminalPROSITE-ProRule annotationAdd BLAST79

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni235 – 2963 X approximate tandem repeatsAdd BLAST62
Regioni523 – 819Interaction with SUPT6H and ALYREF1 PublicationAdd BLAST297

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi83 – 509Glu-richAdd BLAST427

Sequence similaritiesi

Belongs to the IWS1 family.Curated
Contains 1 TFIIS N-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1793. Eukaryota.
COG5139. LUCA.
GeneTreeiENSGT00720000108834.
HOVERGENiHBG081811.
InParanoidiQ96ST2.
KOiK17498.
OMAiRHQASDS.
OrthoDBiEOG091G0K1Z.
PhylomeDBiQ96ST2.
TreeFamiTF315504.

Family and domain databases

Gene3Di1.20.930.10. 1 hit.
InterProiIPR017923. TFIIS_N.
[Graphical view]
PfamiPF08711. Med26. 1 hit.
[Graphical view]
SUPFAMiSSF47676. SSF47676. 1 hit.
PROSITEiPS51319. TFIIS_N. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96ST2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDSEYYSGDQ SDDGGATPVQ DERDSGSDGE DDVNEQHSGS DTGSVERHSE
60 70 80 90 100
NETSDREDGL PKGHHVTDSE NDEPLNLNAS DSESEELHRQ KDSDSESEER
110 120 130 140 150
AEPPASDSEN EDVNQHGSDS ESEETRKLPG SDSENEELLN GHASDSENED
160 170 180 190 200
VGKHPASDSE IEELQKSPAS DSETEDALKP QISDSESEEP PRHQASDSEN
210 220 230 240 250
EEPPKPRMSD SESEELPKPQ VSDSESEEPP RHQASDSENE ELPKPRISDS
260 270 280 290 300
ESEDPPRHQA SDSENEELPK PRISDSESED PPRNQASDSE NEELPKPRVS
310 320 330 340 350
DSESEGPQKG PASDSETEDA SRHKQKPESD DDSDRENKGE DTEMQNDSFH
360 370 380 390 400
SDSHMDRKKF HSSDSEEEEH KKQKMDSDED EKEGEEEKVA KRKAAVLSDS
410 420 430 440 450
EDEEKASAKK SRVVSDADDS DSDAVSDKSG KREKTIASDS EEEAGKELSD
460 470 480 490 500
KKNEEKDLFG SDSESGNEEE NLIADIFGES GDEEEEEFTG FNQEDLEEEK
510 520 530 540 550
GETQVKEAED SDSDDNIKRG KHMDFLSDFE MMLQRKKSMS GKRRRNRDGG
560 570 580 590 600
TFISDADDVV SAMIVKMNEA AEEDRQLNNQ KKPALKKLTL LPAVVMHLKK
610 620 630 640 650
QDLKETFIDS GVMSAIKEWL SPLPDRSLPA LKIREELLKI LQELPSVSQE
660 670 680 690 700
TLKHSGIGRA VMYLYKHPKE SRSNKDMAGK LINEWSRPIF GLTSNYKGMT
710 720 730 740 750
REEREQRDLE QMPQRRRMNS TGGQTPRRDL EKVLTGEEKA LRPGDPGFCA
760 770 780 790 800
RARVPMPSNK DYVVRPKWNV EMESSRFQAT SKKGISRLDK QMRKFTDIRK
810
KSRSAHAVKI SIEGNKMPL
Length:819
Mass (Da):91,955
Last modified:January 10, 2006 - v2
Checksum:iC9749A2C2DC05D72
GO
Isoform 2 (identifier: Q96ST2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     20-344: Missing.

Note: No experimental confirmation available.
Show »
Length:494
Mass (Da):56,109
Checksum:i350E71E75B0B06DE
GO
Isoform 3 (identifier: Q96ST2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     54-260: Missing.

Note: No experimental confirmation available.
Show »
Length:612
Mass (Da):69,108
Checksum:i6A011627CCFAE58B
GO

Sequence cautioni

The sequence AAH65279 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence BAA91858 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti244K → R in BAB55198 (PubMed:14702039).Curated1
Sequence conflicti530E → G in BAA91402 (PubMed:14702039).Curated1
Sequence conflicti559V → D in BAB55198 (PubMed:14702039).Curated1
Sequence conflicti786S → G in BAB55198 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_055975390A → V.Corresponds to variant rs34377117dbSNPEnsembl.1
Natural variantiVAR_055976425V → I.Corresponds to variant rs34785867dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01699220 – 344Missing in isoform 2. 1 PublicationAdd BLAST325
Alternative sequenceiVSP_01699354 – 260Missing in isoform 3. 1 PublicationAdd BLAST207

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000868 mRNA. Translation: BAA91402.1.
AK001717 mRNA. Translation: BAA91858.1. Different initiation.
AK027561 mRNA. Translation: BAB55198.1.
AK056881 mRNA. Translation: BAB71301.1.
AL834178 mRNA. Translation: CAD38875.1.
BC065279 mRNA. Translation: AAH65279.1. Sequence problems.
BC110536 mRNA. Translation: AAI10537.1.
BC110537 mRNA. Translation: AAI10538.1.
CCDSiCCDS2146.1. [Q96ST2-1]
RefSeqiNP_060439.2. NM_017969.2. [Q96ST2-1]
UniGeneiHs.469879.
Hs.679491.

Genome annotation databases

EnsembliENST00000295321; ENSP00000295321; ENSG00000163166. [Q96ST2-1]
GeneIDi55677.
KEGGihsa:55677.
UCSCiuc002ton.3. human. [Q96ST2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000868 mRNA. Translation: BAA91402.1.
AK001717 mRNA. Translation: BAA91858.1. Different initiation.
AK027561 mRNA. Translation: BAB55198.1.
AK056881 mRNA. Translation: BAB71301.1.
AL834178 mRNA. Translation: CAD38875.1.
BC065279 mRNA. Translation: AAH65279.1. Sequence problems.
BC110536 mRNA. Translation: AAI10537.1.
BC110537 mRNA. Translation: AAI10538.1.
CCDSiCCDS2146.1. [Q96ST2-1]
RefSeqiNP_060439.2. NM_017969.2. [Q96ST2-1]
UniGeneiHs.469879.
Hs.679491.

3D structure databases

ProteinModelPortaliQ96ST2.
SMRiQ96ST2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120807. 33 interactors.
IntActiQ96ST2. 22 interactors.
STRINGi9606.ENSP00000295321.

PTM databases

iPTMnetiQ96ST2.
PhosphoSitePlusiQ96ST2.

Polymorphism and mutation databases

BioMutaiIWS1.
DMDMi85542184.

Proteomic databases

EPDiQ96ST2.
MaxQBiQ96ST2.
PaxDbiQ96ST2.
PeptideAtlasiQ96ST2.
PRIDEiQ96ST2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000295321; ENSP00000295321; ENSG00000163166. [Q96ST2-1]
GeneIDi55677.
KEGGihsa:55677.
UCSCiuc002ton.3. human. [Q96ST2-1]

Organism-specific databases

CTDi55677.
GeneCardsiIWS1.
HGNCiHGNC:25467. IWS1.
HPAiHPA035719.
HPA061866.
neXtProtiNX_Q96ST2.
OpenTargetsiENSG00000163166.
PharmGKBiPA144596419.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1793. Eukaryota.
COG5139. LUCA.
GeneTreeiENSGT00720000108834.
HOVERGENiHBG081811.
InParanoidiQ96ST2.
KOiK17498.
OMAiRHQASDS.
OrthoDBiEOG091G0K1Z.
PhylomeDBiQ96ST2.
TreeFamiTF315504.

Enzyme and pathway databases

ReactomeiR-HSA-112382. Formation of RNA Pol II elongation complex.
R-HSA-112387. Elongation arrest and recovery.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-75955. RNA Polymerase II Transcription Elongation.

Miscellaneous databases

ChiTaRSiIWS1. human.
GeneWikiiIWS1.
GenomeRNAii55677.
PROiQ96ST2.

Gene expression databases

BgeeiENSG00000163166.
CleanExiHS_IWS1.
ExpressionAtlasiQ96ST2. baseline and differential.
GenevisibleiQ96ST2. HS.

Family and domain databases

Gene3Di1.20.930.10. 1 hit.
InterProiIPR017923. TFIIS_N.
[Graphical view]
PfamiPF08711. Med26. 1 hit.
[Graphical view]
SUPFAMiSSF47676. SSF47676. 1 hit.
PROSITEiPS51319. TFIIS_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIWS1_HUMAN
AccessioniPrimary (citable) accession number: Q96ST2
Secondary accession number(s): Q2TB65
, Q6P157, Q8N3E8, Q96MI7, Q9NV97, Q9NWH8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: January 10, 2006
Last modified: November 30, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.