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Protein

Protein IWS1 homolog

Gene

IWS1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor which plays a key role in defining the composition of the RNA polymerase II (RNAPII) elongation complex and in modulating the production of mature mRNA transcripts. Acts as an assembly factor to recruit various factors to the RNAPII elongation complex and is recruited to the complex via binding to the transcription elongation factor SUPT6H bound to the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2) to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription.3 Publications

GO - Molecular functioni

GO - Biological processi

  • mRNA processing Source: UniProtKB-KW
  • mRNA transport Source: UniProtKB-KW
  • regulation of histone H3-K36 trimethylation Source: UniProtKB
  • regulation of histone H4 acetylation Source: UniProtKB
  • regulation of mRNA export from nucleus Source: UniProtKB
  • regulation of mRNA processing Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • RNA splicing Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing, mRNA transport, Transcription, Transcription regulation, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Protein IWS1 homolog
Alternative name(s):
IWS1-like protein
Gene namesi
Name:IWS1
Synonyms:IWS1L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:25467. IWS1.

Subcellular locationi

GO - Cellular componenti

  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA144596419.

Polymorphism and mutation databases

BioMutaiIWS1.
DMDMi85542184.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 819819Protein IWS1 homologPRO_0000083346Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineCombined sources
Modified residuei27 – 271PhosphoserineBy similarity
Modified residuei54 – 541PhosphoserineCombined sources
Modified residuei69 – 691PhosphoserineCombined sources
Modified residuei157 – 1571PhosphoserineCombined sources
Modified residuei159 – 1591PhosphoserineCombined sources
Modified residuei183 – 1831PhosphoserineCombined sources
Modified residuei196 – 1961PhosphoserineCombined sources
Modified residuei198 – 1981PhosphoserineCombined sources
Modified residuei209 – 2091PhosphoserineBy similarity
Modified residuei235 – 2351PhosphoserineCombined sources
Modified residuei237 – 2371PhosphoserineCombined sources
Modified residuei248 – 2481PhosphoserineCombined sources
Modified residuei250 – 2501PhosphoserineCombined sources
Modified residuei261 – 2611PhosphoserineCombined sources
Modified residuei263 – 2631PhosphoserineCombined sources
Modified residuei274 – 2741PhosphoserineCombined sources
Modified residuei276 – 2761PhosphoserineCombined sources
Modified residuei287 – 2871PhosphoserineCombined sources
Modified residuei289 – 2891PhosphoserineCombined sources
Modified residuei300 – 3001PhosphoserineCombined sources
Modified residuei302 – 3021PhosphoserineCombined sources
Modified residuei304 – 3041PhosphoserineCombined sources
Modified residuei313 – 3131PhosphoserineCombined sources
Modified residuei315 – 3151PhosphoserineCombined sources
Modified residuei329 – 3291PhosphoserineCombined sources
Modified residuei333 – 3331PhosphoserineCombined sources
Modified residuei351 – 3511PhosphoserineBy similarity
Modified residuei362 – 3621PhosphoserineBy similarity
Modified residuei363 – 3631PhosphoserineCombined sources
Modified residuei365 – 3651PhosphoserineCombined sources
Modified residuei377 – 3771PhosphoserineCombined sources
Modified residuei398 – 3981PhosphoserineCombined sources
Modified residuei400 – 4001PhosphoserineCombined sources
Modified residuei415 – 4151PhosphoserineCombined sources
Modified residuei420 – 4201PhosphoserineCombined sources
Modified residuei422 – 4221PhosphoserineCombined sources
Modified residuei426 – 4261PhosphoserineBy similarity
Modified residuei435 – 4351PhosphothreonineCombined sources
Modified residuei438 – 4381PhosphoserineCombined sources
Modified residuei440 – 4401PhosphoserineCombined sources
Modified residuei480 – 4801PhosphoserineCombined sources
Modified residuei511 – 5111PhosphoserineCombined sources
Modified residuei513 – 5131PhosphoserineCombined sources
Modified residuei725 – 7251PhosphothreonineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ96ST2.
MaxQBiQ96ST2.
PaxDbiQ96ST2.
PeptideAtlasiQ96ST2.
PRIDEiQ96ST2.

PTM databases

iPTMnetiQ96ST2.
PhosphoSiteiQ96ST2.

Expressioni

Gene expression databases

BgeeiENSG00000163166.
CleanExiHS_IWS1.
ExpressionAtlasiQ96ST2. baseline and differential.
GenevisibleiQ96ST2. HS.

Organism-specific databases

HPAiHPA035719.
HPA061866.

Interactioni

Subunit structurei

Interacts with SUPT6H; binds preferentially to the POLR2A-bound SUPT6H. Interacts with ALYREF/THOC4, SETD2 and PRMT5. Interacts with HDGFRP2.4 Publications

Protein-protein interaction databases

BioGridi120807. 33 interactions.
IntActiQ96ST2. 22 interactions.
STRINGi9606.ENSP00000295321.

Structurei

3D structure databases

ProteinModelPortaliQ96ST2.
SMRiQ96ST2. Positions 584-688.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati235 – 260261Add
BLAST
Repeati261 – 286262Add
BLAST
Repeati287 – 296103; half-length
Domaini614 – 69279TFIIS N-terminalPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni235 – 296623 X approximate tandem repeatsAdd
BLAST
Regioni523 – 819297Interaction with SUPT6H and ALYREFAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi83 – 509427Glu-richAdd
BLAST

Sequence similaritiesi

Belongs to the IWS1 family.Curated
Contains 1 TFIIS N-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1793. Eukaryota.
COG5139. LUCA.
GeneTreeiENSGT00720000108834.
HOVERGENiHBG081811.
InParanoidiQ96ST2.
KOiK17498.
OMAiRHQASDS.
OrthoDBiEOG091G0K1Z.
PhylomeDBiQ96ST2.
TreeFamiTF315504.

Family and domain databases

Gene3Di1.20.930.10. 1 hit.
InterProiIPR017923. TFIIS_N.
[Graphical view]
PfamiPF08711. Med26. 1 hit.
[Graphical view]
SUPFAMiSSF47676. SSF47676. 1 hit.
PROSITEiPS51319. TFIIS_N. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96ST2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDSEYYSGDQ SDDGGATPVQ DERDSGSDGE DDVNEQHSGS DTGSVERHSE
60 70 80 90 100
NETSDREDGL PKGHHVTDSE NDEPLNLNAS DSESEELHRQ KDSDSESEER
110 120 130 140 150
AEPPASDSEN EDVNQHGSDS ESEETRKLPG SDSENEELLN GHASDSENED
160 170 180 190 200
VGKHPASDSE IEELQKSPAS DSETEDALKP QISDSESEEP PRHQASDSEN
210 220 230 240 250
EEPPKPRMSD SESEELPKPQ VSDSESEEPP RHQASDSENE ELPKPRISDS
260 270 280 290 300
ESEDPPRHQA SDSENEELPK PRISDSESED PPRNQASDSE NEELPKPRVS
310 320 330 340 350
DSESEGPQKG PASDSETEDA SRHKQKPESD DDSDRENKGE DTEMQNDSFH
360 370 380 390 400
SDSHMDRKKF HSSDSEEEEH KKQKMDSDED EKEGEEEKVA KRKAAVLSDS
410 420 430 440 450
EDEEKASAKK SRVVSDADDS DSDAVSDKSG KREKTIASDS EEEAGKELSD
460 470 480 490 500
KKNEEKDLFG SDSESGNEEE NLIADIFGES GDEEEEEFTG FNQEDLEEEK
510 520 530 540 550
GETQVKEAED SDSDDNIKRG KHMDFLSDFE MMLQRKKSMS GKRRRNRDGG
560 570 580 590 600
TFISDADDVV SAMIVKMNEA AEEDRQLNNQ KKPALKKLTL LPAVVMHLKK
610 620 630 640 650
QDLKETFIDS GVMSAIKEWL SPLPDRSLPA LKIREELLKI LQELPSVSQE
660 670 680 690 700
TLKHSGIGRA VMYLYKHPKE SRSNKDMAGK LINEWSRPIF GLTSNYKGMT
710 720 730 740 750
REEREQRDLE QMPQRRRMNS TGGQTPRRDL EKVLTGEEKA LRPGDPGFCA
760 770 780 790 800
RARVPMPSNK DYVVRPKWNV EMESSRFQAT SKKGISRLDK QMRKFTDIRK
810
KSRSAHAVKI SIEGNKMPL
Length:819
Mass (Da):91,955
Last modified:January 10, 2006 - v2
Checksum:iC9749A2C2DC05D72
GO
Isoform 2 (identifier: Q96ST2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     20-344: Missing.

Note: No experimental confirmation available.
Show »
Length:494
Mass (Da):56,109
Checksum:i350E71E75B0B06DE
GO
Isoform 3 (identifier: Q96ST2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     54-260: Missing.

Note: No experimental confirmation available.
Show »
Length:612
Mass (Da):69,108
Checksum:i6A011627CCFAE58B
GO

Sequence cautioni

The sequence AAH65279 differs from that shown.Contaminating sequence. Potential poly-A sequence.Curated
The sequence BAA91858 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti244 – 2441K → R in BAB55198 (PubMed:14702039).Curated
Sequence conflicti530 – 5301E → G in BAA91402 (PubMed:14702039).Curated
Sequence conflicti559 – 5591V → D in BAB55198 (PubMed:14702039).Curated
Sequence conflicti786 – 7861S → G in BAB55198 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti390 – 3901A → V.
Corresponds to variant rs34377117 [ dbSNP | Ensembl ].
VAR_055975
Natural varianti425 – 4251V → I.
Corresponds to variant rs34785867 [ dbSNP | Ensembl ].
VAR_055976

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei20 – 344325Missing in isoform 2. 1 PublicationVSP_016992Add
BLAST
Alternative sequencei54 – 260207Missing in isoform 3. 1 PublicationVSP_016993Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000868 mRNA. Translation: BAA91402.1.
AK001717 mRNA. Translation: BAA91858.1. Different initiation.
AK027561 mRNA. Translation: BAB55198.1.
AK056881 mRNA. Translation: BAB71301.1.
AL834178 mRNA. Translation: CAD38875.1.
BC065279 mRNA. Translation: AAH65279.1. Sequence problems.
BC110536 mRNA. Translation: AAI10537.1.
BC110537 mRNA. Translation: AAI10538.1.
CCDSiCCDS2146.1. [Q96ST2-1]
RefSeqiNP_060439.2. NM_017969.2. [Q96ST2-1]
UniGeneiHs.469879.
Hs.679491.

Genome annotation databases

EnsembliENST00000295321; ENSP00000295321; ENSG00000163166. [Q96ST2-1]
GeneIDi55677.
KEGGihsa:55677.
UCSCiuc002ton.3. human. [Q96ST2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000868 mRNA. Translation: BAA91402.1.
AK001717 mRNA. Translation: BAA91858.1. Different initiation.
AK027561 mRNA. Translation: BAB55198.1.
AK056881 mRNA. Translation: BAB71301.1.
AL834178 mRNA. Translation: CAD38875.1.
BC065279 mRNA. Translation: AAH65279.1. Sequence problems.
BC110536 mRNA. Translation: AAI10537.1.
BC110537 mRNA. Translation: AAI10538.1.
CCDSiCCDS2146.1. [Q96ST2-1]
RefSeqiNP_060439.2. NM_017969.2. [Q96ST2-1]
UniGeneiHs.469879.
Hs.679491.

3D structure databases

ProteinModelPortaliQ96ST2.
SMRiQ96ST2. Positions 584-688.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120807. 33 interactions.
IntActiQ96ST2. 22 interactions.
STRINGi9606.ENSP00000295321.

PTM databases

iPTMnetiQ96ST2.
PhosphoSiteiQ96ST2.

Polymorphism and mutation databases

BioMutaiIWS1.
DMDMi85542184.

Proteomic databases

EPDiQ96ST2.
MaxQBiQ96ST2.
PaxDbiQ96ST2.
PeptideAtlasiQ96ST2.
PRIDEiQ96ST2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000295321; ENSP00000295321; ENSG00000163166. [Q96ST2-1]
GeneIDi55677.
KEGGihsa:55677.
UCSCiuc002ton.3. human. [Q96ST2-1]

Organism-specific databases

CTDi55677.
GeneCardsiIWS1.
HGNCiHGNC:25467. IWS1.
HPAiHPA035719.
HPA061866.
neXtProtiNX_Q96ST2.
PharmGKBiPA144596419.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1793. Eukaryota.
COG5139. LUCA.
GeneTreeiENSGT00720000108834.
HOVERGENiHBG081811.
InParanoidiQ96ST2.
KOiK17498.
OMAiRHQASDS.
OrthoDBiEOG091G0K1Z.
PhylomeDBiQ96ST2.
TreeFamiTF315504.

Miscellaneous databases

ChiTaRSiIWS1. human.
GeneWikiiIWS1.
GenomeRNAii55677.
PROiQ96ST2.

Gene expression databases

BgeeiENSG00000163166.
CleanExiHS_IWS1.
ExpressionAtlasiQ96ST2. baseline and differential.
GenevisibleiQ96ST2. HS.

Family and domain databases

Gene3Di1.20.930.10. 1 hit.
InterProiIPR017923. TFIIS_N.
[Graphical view]
PfamiPF08711. Med26. 1 hit.
[Graphical view]
SUPFAMiSSF47676. SSF47676. 1 hit.
PROSITEiPS51319. TFIIS_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIWS1_HUMAN
AccessioniPrimary (citable) accession number: Q96ST2
Secondary accession number(s): Q2TB65
, Q6P157, Q8N3E8, Q96MI7, Q9NV97, Q9NWH8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: January 10, 2006
Last modified: September 7, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.