Q96SN7 (ORAI2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein orai-2

Alternative name(s):
CAP-binding protein complex-interacting protein 2
Transmembrane protein 142B

Gene names

Name:	ORAI2
Synonyms:	C7orf19, CBCIP2, TMEM142B
ORF Names:	PP1729

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ca2+ release-activated Ca2+-like (CRAC-like) channel subunit which mediates Ca2+ influx and increase in Ca2+-selective current by synergy with the Ca2+ sensor, STIM1. Ref.6 Ref.8
Subunit structure	Interacts with EFCAB4B/CRACR2A. Ref.9
Subcellular location	Membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the Orai family.
Sequence caution	The sequence AAG23755.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS. The sequence AAP22352.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAG33568.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 254	254	Protein orai-2		PRO_0000234391
Regions
	Transmembrane	66 – 83	18	Helical; Potential
	Transmembrane	94 – 114	21	Helical; Potential
	Transmembrane	148 – 168	21	Helical; Potential
	Transmembrane	196 – 216	21	Helical; Potential
Natural variations
	Natural variant	15	1	A → P. Corresponds to variant rs34947403 [ dbSNP | Ensembl ].		VAR_053543

Sequences

	Sequence		Length	Mass (Da)
	Q96SN7 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: D53CD2376F48E23C	FASTA	254	28,570
	10 20 30 40 50 60 MSAELNVPID PSAPACPEPG HKGMDYRDWV RRSYLELVTS NHHSVQALSW RKLYLSRAKL 70 80 90 100 110 120 KASSRTSALL SGFAMVAMVE VQLETQYQYP RPLLIAFSAC TTVLVAVHLF ALLISTCILP 130 140 150 160 170 180 NVEAVSNIHN LNSISESPHE RMHPYIELAW GFSTVLGILL FLAEVVLLCW IKFLPVDARR 190 200 210 220 230 240 QPGPPPGPGS HTGWQAALVS TIIMVPVGLI FVVFTIHFYR SLVRHKTERH NREIEELHKL 250 KVQLDGHERS LQVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Teratocarcinoma.
[2]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"Large-scale cDNA transfection screening for genes related to cancer development and progression." Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 191-254.
[5]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: PARTIAL NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1." Mercer J.C., Dehaven W.I., Smyth J.T., Wedel B., Boyles R.R., Bird G.S., Putney J.W. Jr. J. Biol. Chem. 281:24979-24990(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function." Feske S., Gwack Y., Prakriya M., Srikanth S., Puppel S.-H., Tanasa B., Hogan P.G., Lewis R.S., Daly M., Rao A. Nature 441:179-185(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[8]	"Calcium inhibition and calcium potentiation of orai1, orai2, and orai3 calcium release-activated calcium channels." Dehaven W.I., Smyth J.T., Boyles R.R., Putney J.W. Jr. J. Biol. Chem. 282:17548-17556(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that stabilizes CRAC channels in T cells." Srikanth S., Jung H.J., Kim K.D., Souda P., Whitelegge J., Gwack Y. Nat. Cell Biol. 12:436-446(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH EFCAB4B.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK027639 mRNA. Translation: BAB55256.1. AC093668 Genomic DNA. Translation: AAP22351.1. AC093668 Genomic DNA. Translation: AAP22352.1. Sequence problems. BC069270 mRNA. Translation: AAH69270.1. AF258552 mRNA. Translation: AAG23755.1. Sequence problems. CR457287 mRNA. Translation: CAG33568.1. Sequence problems.
IPI	IPI00045842.
RefSeq	NP_001119812.1. NM_001126340.2. NP_001258747.1. NM_001271818.1. NP_116220.1. NM_032831.3.
UniGene	Hs.363308.
3D structure databases
ProteinModelPortal	Q96SN7.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48944N.
IntAct	Q96SN7. 7 interactions.
MINT	MINT-1373233.
STRING	9606.ENSP00000348752.
PTM databases
PhosphoSite	Q96SN7.
Polymorphism databases
DMDM	74732728.
Proteomic databases
PaxDb	Q96SN7.
PRIDE	Q96SN7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000356387; ENSP00000348752; ENSG00000160991. ENST00000403646; ENSP00000385489; ENSG00000160991. ENST00000473939; ENSP00000417928; ENSG00000160991. ENST00000478730; ENSP00000418140; ENSG00000160991.
GeneID	80228.
KEGG	hsa:80228.
UCSC	uc003uzj.2. human.
Organism-specific databases
CTD	80228.
GeneCards	GC07P102074.
HGNC	HGNC:21667. ORAI2.
MIM	610929. gene.
neXtProt	NX_Q96SN7.
PharmGKB	PA162398454.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG263788.
HOGENOM	HOG000246925.
HOVERGEN	HBG081343.
InParanoid	Q96SN7.
KO	K16057.
OMA	PACSEPG.
OrthoDB	EOG4DV5MR.
Gene expression databases
ArrayExpress	Q96SN7.
Bgee	Q96SN7.
CleanEx	HS_ORAI2.
Genevestigator	Q96SN7.
GermOnline	ENSG00000160991. Homo sapiens.
Family and domain databases
InterPro	IPR012446. CRAC_channel. [Graphical view]
Pfam	PF07856. Orai-1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	ORAI2. human.
GenomeRNAi	80228.
NextBio	70657.
SOURCE	Search...

Entry information

Entry name

ORAI2_HUMAN

Accession

Primary (citable) accession number: Q96SN7
Secondary accession number(s): Q6IA68, Q8WY94, Q9H9Y3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	December 1, 2001
Last modified:	April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.