Q96SI9 (STRBP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spermatid perinuclear RNA-binding protein

Gene names

Name:	STRBP
Synonyms:	SPNR

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	672 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in spermatogenesis and sperm function. Plays a role in regulation of cell growth. Binds to double-stranded DNA and RNA. Binds most efficiently to poly(I:C) RNA than to poly(dI:dC) DNA. Binds also to single-stranded poly(G) RNA. Binds non-specifically to the mRNA PRM1 3'-UTR and adenovirus VA RNA By similarity.
Subunit structure	Interacts with EIF2AK2 By similarity. Associates with microtubules; it is unsure whether such interaction is direct or indirect By similarity.
Subcellular location	Cytoplasm By similarity. Note: Microtubule-associated that localizes to the manchette in developing spermatids By similarity.
Sequence similarities	Contains 2 DRBM (double-stranded RNA-binding) domains. Contains 1 DZF domain.
Sequence caution	The sequence AAI08732.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence BAA92120.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB14873.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Differentiation Spermatogenesis
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat
Ligand	DNA-binding RNA-binding
Molecular function	Developmental protein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular component movement Inferred from electronic annotation. Source: Compara mechanosensory behavior Inferred from electronic annotation. Source: Compara multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW spermatid development Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule cytoskeleton Inferred from electronic annotation. Source: Compara nucleus Inferred from direct assay. Source: LIFEdb
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW double-stranded RNA binding Inferred from electronic annotation. Source: Compara single-stranded RNA binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96SI9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96SI9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-14: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 672

672

Spermatid perinuclear RNA-binding protein

PRO_0000274917

Regions

Domain

87 – 334

248

DZF

Domain

387 – 453

67

DRBM 1

Domain

510 – 576

67

DRBM 2

Compositional bias

584 – 587

4

Poly-Lys

Natural variations

Alternative sequence

1 – 14

14

Missing in isoform 2.

VSP_022937

Natural variant

280

1

G → R in a breast cancer sample; somatic mutation. Ref.9

VAR_035662

Experimental info

Sequence conflict

173 – 175

3

LEK → IGRR in AAK20832. Ref.1

Sequence conflict

196

1

L → P in BAA92120. Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

672

Helix Strand Turn

Details...

Beta strand

385 – 387

3

Helix

390 – 398

9

Beta strand

405 – 411

7

Beta strand

413 – 415

3

Beta strand

417 – 424

8

Beta strand

427 – 435

9

Helix

436 – 451

16

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 26465F4D3A429FBC	FASTA	672	73,653
	10 20 30 40 50 60 MRSIRSFAND DRHVMVKHST IYPSPEELEA VQNMVSTVEC ALKHVSDWLD ETNKGTKTEG 70 80 90 100 110 120 ETEVKKDEAG ENYSKDQGGR TLCGVMRIGL VAKGLLIKDD MDLELVLMCK DKPTETLLNT 130 140 150 160 170 180 VKDNLPIQIQ KLTEEKYQVE QCVNEASIII RNTKEPTLTL KVILTSPLIR DELEKKDGEN 190 200 210 220 230 240 VSMKDPPDLL DRQKCLNALA SLRHAKWFQA RANGLKSCVI VLRILRDLCN RVPTWAPLKG 250 260 270 280 290 300 WPLELICEKS IGTCNRPLGA GEALRRVMEC LASGILLPGG PGLHDPCERD PTDALSYMTI 310 320 330 340 350 360 QQKEDITHSA QHALRLSAFG QIYKVLEMDP LPSSKPFQKY SWSVTDKEGA GSSALKRPFE 370 380 390 400 410 420 DGLGDDKDPN KKMKRNLRKI LDSKAIDLMN ALMRLNQIRP GLQYKLLSQS GPVHAPVFTM 430 440 450 460 470 480 SVDVDGTTYE ASGPSKKTAK LHVAVKVLQA MGYPTGFDAD IECMSSDEKS DNESKNETVS 490 500 510 520 530 540 SNSSNNTGNS TTETSSTLEV RTQGPILTAS GKNPVMELNE KRRGLKYELI SETGGSHDKR 550 560 570 580 590 600 FVMEVEVDGQ KFRGAGPNKK VAKASAALAA LEKLFSGPNA ANNKKKKIIP QAKGVVNTAV 610 620 630 640 650 660 SAAVQAVRGR GRGTLTRGAF VGATAAPGYI APGYGTPYGY STAAPAYGLP KRMVLLPVMK 670 FPTYPVPHYS FF
	Isoform 2 [UniParc]. Checksum: 131F93237EEF70B1	FASTA	658	71,967
	10 20 30 40 50 60 MVKHSTIYPS PEELEAVQNM VSTVECALKH VSDWLDETNK GTKTEGETEV KKDEAGENYS 70 80 90 100 110 120 KDQGGRTLCG VMRIGLVAKG LLIKDDMDLE LVLMCKDKPT ETLLNTVKDN LPIQIQKLTE 130 140 150 160 170 180 EKYQVEQCVN EASIIIRNTK EPTLTLKVIL TSPLIRDELE KKDGENVSMK DPPDLLDRQK 190 200 210 220 230 240 CLNALASLRH AKWFQARANG LKSCVIVLRI LRDLCNRVPT WAPLKGWPLE LICEKSIGTC 250 260 270 280 290 300 NRPLGAGEAL RRVMECLASG ILLPGGPGLH DPCERDPTDA LSYMTIQQKE DITHSAQHAL 310 320 330 340 350 360 RLSAFGQIYK VLEMDPLPSS KPFQKYSWSV TDKEGAGSSA LKRPFEDGLG DDKDPNKKMK 370 380 390 400 410 420 RNLRKILDSK AIDLMNALMR LNQIRPGLQY KLLSQSGPVH APVFTMSVDV DGTTYEASGP 430 440 450 460 470 480 SKKTAKLHVA VKVLQAMGYP TGFDADIECM SSDEKSDNES KNETVSSNSS NNTGNSTTET 490 500 510 520 530 540 SSTLEVRTQG PILTASGKNP VMELNEKRRG LKYELISETG GSHDKRFVME VEVDGQKFRG 550 560 570 580 590 600 AGPNKKVAKA SAALAALEKL FSGPNAANNK KKKIIPQAKG VVNTAVSAAV QAVRGRGRGT 610 620 630 640 650 LTRGAFVGAT AAPGYIAPGY GTPYGYSTAA PAYGLPKRMV LLPVMKFPTY PVPHYSFF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Sha J.H., Zhou Z.M., Li J.M. Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Testis.
[2]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Testis.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Placenta and Retinoblastoma.
[4]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Leiomyosarcoma, Retinoblastoma and Uterus.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]	"Solution structure of DSRM domain in spermatid perinuclear RNA-binding protein." RIKEN structural genomics initiative (RSGI) Submitted (OCT-2006) to the PDB data bank Cited for: STRUCTURE BY NMR OF 378-461.
[9]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] ARG-280.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF333337 mRNA. Translation: AAK20832.1.
AL136866 mRNA. Translation: CAB66800.1.
AK002169 mRNA. Translation: BAA92120.1. Different initiation.
AK024285 mRNA. Translation: BAB14873.1. Different initiation.
AK027890 mRNA. Translation: BAB55434.1.
AL365338 Genomic DNA. Translation: CAH70299.1.
AL365338 Genomic DNA. Translation: CAH70300.1.
BC002693 mRNA. Translation: AAH02693.2.
BC017732 mRNA. Translation: AAH17732.1.
BC108731 mRNA. Translation: AAI08732.1. Sequence problems.

IPI

IPI00169430.
IPI00413860.

RefSeq

NP_001164608.1. NM_001171137.1.
NP_060857.2. NM_018387.4.

UniGene

Hs.287659.
Hs.694157.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2DMY	NMR	-	A	378-461	[»]

ProteinModelPortal

Q96SI9.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q96SI9. 6 interactions.

MINT

MINT-1434039.

STRING

9606.ENSP00000321347.

PTM databases

PhosphoSite

Q96SI9.

Polymorphism databases

DMDM

74752134.

Proteomic databases

PaxDb

Q96SI9.

PRIDE

Q96SI9.

Protocols and materials databases

DNASU

55342.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000348403; ENSP00000321347; ENSG00000165209.
ENST00000360998; ENSP00000354271; ENSG00000165209.
ENST00000447404; ENSP00000415968; ENSG00000165209.

GeneID

55342.

KEGG

hsa:55342.

UCSC

uc004bns.3. human.

Organism-specific databases

CTD

55342.

GeneCards

GC09M125871.

HGNC

HGNC:16462. STRBP.

HPA

HPA020651.

MIM

611138. gene.

neXtProt

NX_Q96SI9.

PharmGKB

PA38145.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG269067.

HOVERGEN

HBG069915.

InParanoid

Q96SI9.

KO

K13200.

OMA

TDKEGAG.

OrthoDB

EOG4XGZZN.

PhylomeDB

Q96SI9.

Gene expression databases

ArrayExpress

Q96SI9.

Bgee

Q96SI9.

CleanEx

HS_STRBP.

Genevestigator

Q96SI9.

Family and domain databases

Gene3D

3.30.160.20. 2 hits.

InterPro

IPR001159. Ds-RNA-bd.
IPR014720. dsRNA-bd-like_dom.
IPR006561. DZF.
[Graphical view]

Pfam

PF00035. dsrm. 2 hits.
PF07528. DZF. 1 hit.
[Graphical view]

SMART

SM00358. DSRM. 2 hits.
SM00572. DZF. 1 hit.
[Graphical view]

PROSITE

PS50137. DS_RBD. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

STRBP. human.

EvolutionaryTrace

Q96SI9.

GenomeRNAi

55342.

NextBio

59667.

SOURCE

Search...

Entry information

Entry name

STRBP_HUMAN

Accession

Primary (citable) accession number: Q96SI9
Secondary accession number(s): Q32NB9 , Q9BUE1, Q9BXG4, Q9H0B4, Q9H7V1, Q9NUK4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.