Q96S94 (CCNL2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclin-L2

Alternative name(s):
Paneth cell-enhanced expression protein

Gene names

Name:	CCNL2
ORF Names:	SB138

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional regulator which participates in regulating the pre-mRNA splicing process. Also modulates the expression of critical apoptotic factor, leading to cell apoptosis. Ref.1
Subunit structure	Interacts with CDK11A, CDK11B, CDK12, CDK13, SFRS2, SFRS7 and POLR2A, the hyperphosphorylated C-terminal domain (CTD) of RNA polymerase II. Ref.1
Subcellular location	Nucleus speckle Ref.1.
Tissue specificity	Ubiquitously expressed, with a higher expression level observed in ovary, heart, liver and pancreas. Ref.1
Domain	Contains a RS region (arginine-serine dipeptide repeat) within the C-terminal domain which is the hallmark of the SR family of splicing factors. This region probably plays a role in protein-protein interactions By similarity.
Sequence similarities	Belongs to the cyclin family. Cyclin L subfamily.
Sequence caution	The sequence AAH71622.2 differs from that shown. Reason: Erroneous initiation. The sequence BAB84938.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat
Molecular function	Cyclin
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA processing Inferred from electronic annotation. Source: InterPro regulation of cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nuclear speck Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay PubMed 17494991. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96S94-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96S94-2)

Also known as: CCNL2s;

The sequence of this isoform differs from the canonical sequence as follows:
     221-226: NYMNDS → VASEGK
     227-520: Missing.

Isoform 3 (identifier: Q96S94-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-222: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 520	520	Cyclin-L2		PRO_0000080487
Regions
	Region	83 – 185	103	Cyclin-like 1
	Region	198 – 282	85	Cyclin-like 2
	Region	385 – 423	39	RS
Amino acid modifications
	Modified residue	330	1	Phosphoserine Ref.10 Ref.11 Ref.12
	Modified residue	338	1	Phosphoserine Ref.11
	Modified residue	348	1	Phosphoserine Ref.12
	Modified residue	351	1	Phosphoserine Ref.12
	Modified residue	369	1	Phosphoserine Ref.12
Natural variations
	Alternative sequence	1 – 222	222	Missing in isoform 3.		VSP_016132
	Alternative sequence	221 – 226	6	NYMNDS → VASEGK in isoform 2.		VSP_016130
	Alternative sequence	227 – 520	294	Missing in isoform 2.		VSP_016131
Experimental info
	Sequence conflict	71	1	T → S in AAH16333. Ref.6
	Sequence conflict	324 – 325	2	DG → MS in AAP97201. Ref.8
Isoform 2:
	Sequence conflict	226	1	K → IT in AAM76789. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 4341E55D03D7B0E0	FASTA	520	58,147
	10 20 30 40 50 60 MAAAAAAAGA AGSAAPAAAA GAPGSGGAPS GSQGVLIGDR LYSGVLITLE NCLLPDDKLR 70 80 90 100 110 120 FTPSMSSGLD TDTETDLRVV GCELIQAAGI LLRLPQVAMA TGQVLFQRFF YTKSFVKHSM 130 140 150 160 170 180 EHVSMACVHL ASKIEEAPRR IRDVINVFHR LRQLRDKKKP VPLLLDQDYV NLKNQIIKAE 190 200 210 220 230 240 RRVLKELGFC VHVKHPHKII VMYLQVLECE RNQHLVQTSW NYMNDSLRTD VFVRFQPESI 250 260 270 280 290 300 ACACIYLAAR TLEIPLPNRP HWFLLFGATE EEIQEICLKI LQLYARKKVD LTHLEGEVEK 310 320 330 340 350 360 RKHAIEEAKA QARGLLPGGT QVLDGTSGFS PAPKLVESPK EGKGSKPSPL SVKNTKRRLE 370 380 390 400 410 420 GAKKAKADSP VNGLPKGRES RSRSRSREQS YSRSPSRSAS PKRRKSDSGS TSGGSKSQSR 430 440 450 460 470 480 SRSRSDSPPR QAPRSAPYKG SEIRGSRKSK DCKYPQKPHK SRSRSSSRSR SRSRERADNP 490 500 510 520 GKYKKKSHYY RDQRRERSRS YERTGRRYER DHPGHSRHRR
	Isoform 2 (CCNL2s) [UniParc]. Checksum: 2DA47ABC9FB2FED8	FASTA	226	24,621
	10 20 30 40 50 60 MAAAAAAAGA AGSAAPAAAA GAPGSGGAPS GSQGVLIGDR LYSGVLITLE NCLLPDDKLR 70 80 90 100 110 120 FTPSMSSGLD TDTETDLRVV GCELIQAAGI LLRLPQVAMA TGQVLFQRFF YTKSFVKHSM 130 140 150 160 170 180 EHVSMACVHL ASKIEEAPRR IRDVINVFHR LRQLRDKKKP VPLLLDQDYV NLKNQIIKAE 190 200 210 220 RRVLKELGFC VHVKHPHKII VMYLQVLECE RNQHLVQTSW VASEGK
	Isoform 3 [UniParc]. Checksum: 96FA9D04F9CCB9F7	FASTA	298	33,839
	10 20 30 40 50 60 MNDSLRTDVF VRFQPESIAC ACIYLAARTL EIPLPNRPHW FLLFGATEEE IQEICLKILQ 70 80 90 100 110 120 LYARKKVDLT HLEGEVEKRK HAIEEAKAQA RGLLPGGTQV LDGTSGFSPA PKLVESPKEG 130 140 150 160 170 180 KGSKPSPLSV KNTKRRLEGA KKAKADSPVN GLPKGRESRS RSRSREQSYS RSPSRSASPK 190 200 210 220 230 240 RRKSDSGSTS GGSKSQSRSR SRSDSPPRQA PRSAPYKGSE IRGSRKSKDC KYPQKPHKSR 250 260 270 280 290 SRSSSRSRSR SRERADNPGK YKKKSHYYRD QRRERSRSYE RTGRRYERDH PGHSRHRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cyclin L2, a novel RNA polymerase II-associated cyclin, is involved in pre-mRNA splicing and induces apoptosis of human hepatocellular carcinoma cells." Yang L., Li N., Wang C., Yu Y., Yuan L., Zhang M., Cao X. J. Biol. Chem. 279:11639-11648(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH POLR2A; CDC2L; SFRS2 AND SFRS7. Tissue: Bone marrow.
[2]	"The nucleotide sequence of a long cDNA clone isolated from human spleen." Jikuya H., Takano J., Nomura N., Kikuno R., Nagase T., Ohara O. Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Spleen.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Placenta and Testis.
[4]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Tissue: Bone and Brain.
[7]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 14-226 (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 367-520. Tissue: Amygdala and Melanoma.
[8]	"Cloning of a novel human cDNA homology to murine Paneth cell enhanced expression mRNA." Zhao E.P., Yu L., Wan Y.Z., Tu Q., Zheng L.H., Zhao S.Y. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 324-520.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[10]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-330, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[11]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-330 AND SER-338, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-330; SER-348; SER-351 AND SER-369, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY037150 mRNA. Translation: AAK67631.1. AY116620 mRNA. Translation: AAM76789.1. AK074112 mRNA. Translation: BAB84938.1. Different initiation. AK027770 mRNA. Translation: BAG51376.1. AK292268 mRNA. Translation: BAF84957.1. AL391244 Genomic DNA. Translation: CAI22659.1. AL391244, CR628411 Genomic DNA. Translation: CAI22660.2. CR628411, AL391244 Genomic DNA. Translation: CAM12829.1. CH471183 Genomic DNA. Translation: EAW56213.1. CH471183 Genomic DNA. Translation: EAW56216.1. BC016333 mRNA. Translation: AAH16333.1. BC071622 mRNA. Translation: AAH71622.2. Different initiation. AL834432 mRNA. Translation: CAD39092.1. AL834441 mRNA. Translation: CAD39101.1. AF087903 mRNA. Translation: AAP97201.1.
IPI	IPI00066458. IPI00478642. IPI00787908.
RefSeq	NP_001034666.1. NM_001039577.3. NP_112199.2. NM_030937.4.
UniGene	Hs.515704.
3D structure databases
ProteinModelPortal	Q96S94.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96S94. 1 interaction.
PTM databases
PhosphoSite	Q96S94.
Polymorphism databases
DMDM	74752124.
Proteomic databases
PaxDb	Q96S94.
PRIDE	Q96S94.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000400809; ENSP00000383611; ENSG00000221978. ENST00000408918; ENSP00000386158; ENSG00000221978. ENST00000408952; ENSP00000386132; ENSG00000221978. ENST00000488340; ENSP00000424647; ENSG00000221978.
GeneID	81669.
KEGG	hsa:81669.
UCSC	uc001aff.1. human. uc021oep.1. human.
Organism-specific databases
CTD	81669.
GeneCards	GC01M001321.
HGNC	HGNC:20570. CCNL2.
HPA	HPA053137.
MIM	613482. gene.
neXtProt	NX_Q96S94.
PharmGKB	PA134973681.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5333.
HOVERGEN	HBG056044.
InParanoid	Q96S94.
OMA	TGHRYER.
OrthoDB	EOG4ZPDVD.
Gene expression databases
ArrayExpress	Q96S94.
Bgee	Q96S94.
CleanEx	HS_CCNL2.
Genevestigator	Q96S94.
GermOnline	ENSG00000116148. Homo sapiens.
Family and domain databases
Gene3D	1.10.472.10. 2 hits.
InterPro	IPR013763. Cyclin-like. IPR004367. Cyclin_C-dom. IPR015429. Cyclin_C/H/T/L. IPR017060. Cyclin_L. IPR006671. Cyclin_N. [Graphical view]
PANTHER	PTHR10026. PTHR10026. 1 hit.
Pfam	PF02984. Cyclin_C. 1 hit. PF00134. Cyclin_N. 1 hit. [Graphical view]
PIRSF	PIRSF036580. Cyclin_L. 1 hit.
SMART	SM00385. CYCLIN. 2 hits. [Graphical view]
SUPFAM	SSF47954. Cyclin_like. 2 hits.
PROSITE	PS00292. CYCLINS. False negative. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CCNL2. human.
GenomeRNAi	81669.
NextBio	72022.
SOURCE	Search...

Entry information

Entry name

CCNL2_HUMAN

Accession

Primary (citable) accession number: Q96S94
Secondary accession number(s): A8K8A3 , B1B152, Q5T2N5, Q5T2N6, Q6IQ12, Q7Z4Z8, Q8N3C9, Q8N3D5, Q8NHE3, Q8TEL0, Q96B00

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.