ID   HPLN3_HUMAN             Reviewed;         360 AA.
AC   Q96S86; A8K7P0;
DT   24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   24-JAN-2024, entry version 162.
DE   RecName: Full=Hyaluronan and proteoglycan link protein 3;
DE   Flags: Precursor;
GN   Name=HAPLN3; Synonyms=EXLD1; ORFNames=UNQ238/PRO271;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606 {ECO:0000312|EMBL:AAK67639.1};
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX   PubMed=12663660; DOI=10.1074/jbc.m213100200;
RA   Spicer A.P., Joo A., Bowling R.A. Jr.;
RT   "A hyaluronan binding link protein gene family whose members are physically
RT   linked adjacent to chondroitin sulfate proteoglycan core protein genes: the
RT   missing links.";
RL   J. Biol. Chem. 278:21083-21091(2003).
RN   [2] {ECO:0000312|EMBL:AAK67639.1}
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Li N., Zhang W., Wan T., Zhang M., Cao X.;
RL   Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.
RN   [3] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=12975309; DOI=10.1101/gr.1293003;
RA   Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA   Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA   Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA   Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA   Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA   Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA   Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA   Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT   "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT   identify novel human secreted and transmembrane proteins: a bioinformatics
RT   assessment.";
RL   Genome Res. 13:2265-2270(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Spleen;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [5] {ECO:0000312|EMBL:AAK67639.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [6] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Pancreas {ECO:0000312|EMBL:AAH62320.1};
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: May function in hyaluronic acid binding.
CC       {ECO:0000303|PubMed:12663660}.
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC       matrix {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Widely expressed with highest levels in spleen and
CC       placenta. {ECO:0000269|PubMed:12663660}.
CC   -!- SIMILARITY: Belongs to the HAPLN family. {ECO:0000305}.
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DR   EMBL; AY262759; AAP22051.1; -; mRNA.
DR   EMBL; AY037161; AAK67639.1; -; mRNA.
DR   EMBL; AY358500; AAQ88864.1; -; mRNA.
DR   EMBL; AK292055; BAF84744.1; -; mRNA.
DR   EMBL; CH471101; EAX02022.1; -; Genomic_DNA.
DR   EMBL; BC062320; AAH62320.1; -; mRNA.
DR   CCDS; CCDS10346.1; -.
DR   RefSeq; NP_001294881.1; NM_001307952.1.
DR   RefSeq; NP_839946.1; NM_178232.3.
DR   AlphaFoldDB; Q96S86; -.
DR   SMR; Q96S86; -.
DR   BioGRID; 126950; 56.
DR   IntAct; Q96S86; 16.
DR   STRING; 9606.ENSP00000457180; -.
DR   DrugBank; DB08818; Hyaluronic acid.
DR   iPTMnet; Q96S86; -.
DR   PhosphoSitePlus; Q96S86; -.
DR   BioMuta; HAPLN3; -.
DR   DMDM; 47605734; -.
DR   EPD; Q96S86; -.
DR   jPOST; Q96S86; -.
DR   MassIVE; Q96S86; -.
DR   PaxDb; 9606-ENSP00000352606; -.
DR   PeptideAtlas; Q96S86; -.
DR   ProteomicsDB; 78084; -.
DR   Antibodypedia; 28522; 146 antibodies from 22 providers.
DR   DNASU; 145864; -.
DR   Ensembl; ENST00000359595.8; ENSP00000352606.4; ENSG00000140511.12.
DR   GeneID; 145864; -.
DR   KEGG; hsa:145864; -.
DR   MANE-Select; ENST00000359595.8; ENSP00000352606.4; NM_178232.4; NP_839946.1.
DR   UCSC; uc002bnc.4; human.
DR   AGR; HGNC:21446; -.
DR   CTD; 145864; -.
DR   DisGeNET; 145864; -.
DR   GeneCards; HAPLN3; -.
DR   HGNC; HGNC:21446; HAPLN3.
DR   HPA; ENSG00000140511; Low tissue specificity.
DR   neXtProt; NX_Q96S86; -.
DR   OpenTargets; ENSG00000140511; -.
DR   PharmGKB; PA134919215; -.
DR   VEuPathDB; HostDB:ENSG00000140511; -.
DR   eggNOG; ENOG502QWFF; Eukaryota.
DR   GeneTree; ENSGT00940000159628; -.
DR   HOGENOM; CLU_052285_1_0_1; -.
DR   InParanoid; Q96S86; -.
DR   OrthoDB; 5402504at2759; -.
DR   PhylomeDB; Q96S86; -.
DR   TreeFam; TF332134; -.
DR   PathwayCommons; Q96S86; -.
DR   SignaLink; Q96S86; -.
DR   BioGRID-ORCS; 145864; 11 hits in 1159 CRISPR screens.
DR   ChiTaRS; HAPLN3; human.
DR   GenomeRNAi; 145864; -.
DR   Pharos; Q96S86; Tbio.
DR   PRO; PR:Q96S86; -.
DR   Proteomes; UP000005640; Chromosome 15.
DR   RNAct; Q96S86; Protein.
DR   Bgee; ENSG00000140511; Expressed in descending thoracic aorta and 128 other cell types or tissues.
DR   ExpressionAtlas; Q96S86; baseline and differential.
DR   GO; GO:0005615; C:extracellular space; HDA:UniProtKB.
DR   GO; GO:0072534; C:perineuronal net; IBA:GO_Central.
DR   GO; GO:0045202; C:synapse; IBA:GO_Central.
DR   GO; GO:0005540; F:hyaluronic acid binding; IEA:UniProtKB-KW.
DR   GO; GO:0007155; P:cell adhesion; IEA:InterPro.
DR   GO; GO:0007417; P:central nervous system development; IBA:GO_Central.
DR   GO; GO:0010001; P:glial cell differentiation; IBA:GO_Central.
DR   GO; GO:0002052; P:positive regulation of neuroblast proliferation; IBA:GO_Central.
DR   GO; GO:0001501; P:skeletal system development; IBA:GO_Central.
DR   CDD; cd05877; Ig_LP_like; 1.
DR   CDD; cd03518; Link_domain_HAPLN_module_1; 1.
DR   CDD; cd03519; Link_domain_HAPLN_module_2; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   Gene3D; 3.10.100.10; Mannose-Binding Protein A, subunit A; 2.
DR   InterPro; IPR016186; C-type_lectin-like/link_sf.
DR   InterPro; IPR016187; CTDL_fold.
DR   InterPro; IPR007110; Ig-like_dom.
DR   InterPro; IPR036179; Ig-like_dom_sf.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR003599; Ig_sub.
DR   InterPro; IPR013106; Ig_V-set.
DR   InterPro; IPR000538; Link_dom.
DR   PANTHER; PTHR22804; AGGRECAN/VERSICAN PROTEOGLYCAN; 1.
DR   PANTHER; PTHR22804:SF40; HYALURONAN AND PROTEOGLYCAN LINK PROTEIN 3; 1.
DR   Pfam; PF07686; V-set; 1.
DR   Pfam; PF00193; Xlink; 2.
DR   PRINTS; PR01265; LINKMODULE.
DR   SMART; SM00409; IG; 1.
DR   SMART; SM00406; IGv; 1.
DR   SMART; SM00445; LINK; 2.
DR   SUPFAM; SSF56436; C-type lectin-like; 2.
DR   SUPFAM; SSF48726; Immunoglobulin; 1.
DR   PROSITE; PS50835; IG_LIKE; 1.
DR   PROSITE; PS01241; LINK_1; 2.
DR   PROSITE; PS50963; LINK_2; 2.
DR   Genevisible; Q96S86; HS.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Extracellular matrix; Hyaluronic acid;
KW   Immunoglobulin domain; Reference proteome; Repeat; Secreted; Signal.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000255"
FT   CHAIN           18..360
FT                   /note="Hyaluronan and proteoglycan link protein 3"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000013190"
FT   DOMAIN          48..164
FT                   /note="Ig-like V-type"
FT                   /evidence="ECO:0000305"
FT   DOMAIN          166..261
FT                   /note="Link 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00323,
FT                   ECO:0000305"
FT   DOMAIN          266..358
FT                   /note="Link 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00323,
FT                   ECO:0000305"
FT   DISULFID        70..146
FT                   /evidence="ECO:0000250|UniProtKB:P03994"
FT   DISULFID        188..259
FT                   /evidence="ECO:0000250|UniProtKB:P03994"
FT   DISULFID        212..233
FT                   /evidence="ECO:0000250|UniProtKB:P03994"
FT   DISULFID        286..356
FT                   /evidence="ECO:0000250|UniProtKB:P03994"
FT   DISULFID        311..332
FT                   /evidence="ECO:0000250|UniProtKB:P03994"
SQ   SEQUENCE   360 AA;  40894 MW;  3B72AE88D0D9E8DC CRC64;
     MGLLLLVPLL LLPGSYGLPF YNGFYYSNSA NDQNLGNGHG KDLLNGVKLV VETPEETLFT
     YQGASVILPC RYRYEPALVS PRRVRVKWWK LSENGAPEKD VLVAIGLRHR SFGDYQGRVH
     LRQDKEHDVS LEIQDLRLED YGRYRCEVID GLEDESGLVE LELRGVVFPY QSPNGRYQFN
     FHEGQQVCAE QAAVVASFEQ LFRAWEEGLD WCNAGWLQDA TVQYPIMLPR QPCGGPGLAP
     GVRSYGPRHR RLHRYDVFCF ATALKGRVYY LEHPEKLTLT EAREACQEDD ATIAKVGQLF
     AAWKFHGLDR CDAGWLADGS VRYPVVHPHP NCGPPEPGVR SFGFPDPQSR LYGVYCYRQH
//