Q96S66 (CLCC1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chloride channel CLIC-like protein 1

Alternative name(s):
Mid-1-related chloride channel protein 1

Gene names

Name:	CLCC1
Synonyms:	KIAA0761, MCLC

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	551 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Seems to act as a chloride ion channel By similarity.
Subcellular location	Membrane; Multi-pass membrane protein Potential. Endoplasmic reticulum. Golgi apparatus. Nucleus By similarity.
Sequence similarities	Belongs to the chloride channel MCLC family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum Inferred from direct assay. Source: HPA integral component of membrane Inferred from electronic annotation. Source: UniProtKB-KW intracellular membrane-bounded organelle Inferred from direct assay. Source: HPA nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	chloride channel activity Inferred from electronic annotation. Source: Ensembl
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96S66-1)

Also known as: hMCLC-1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96S66-2)

Also known as: hMCLC-2;

The sequence of this isoform differs from the canonical sequence as follows:
     114-163: Missing.

Isoform 3 (identifier: Q96S66-3)

Also known as: hMCLC-3;

The sequence of this isoform differs from the canonical sequence as follows:
     114-234: Missing.

Isoform 4 (identifier: Q96S66-4)

Also known as: hMCLC-4;

The sequence of this isoform differs from the canonical sequence as follows:
     114-298: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 551	533	Chloride channel CLIC-like protein 1		PRO_0000297682
Regions
	Transmembrane	185 – 205	21	Helical; Potential
	Transmembrane	217 – 237	21	Helical; Potential
	Transmembrane	330 – 350	21	Helical; Potential
Amino acid modifications
	Modified residue	438	1	Phosphoserine Ref.6 Ref.7 Ref.8
	Modified residue	464	1	Phosphoserine Ref.8
	Modified residue	482	1	Phosphothreonine Ref.10
	Modified residue	509	1	Phosphoserine Ref.8 Ref.12
	Modified residue	524	1	Phosphoserine Ref.8 Ref.10
	Modified residue	532	1	Phosphoserine Ref.8 Ref.10
Natural variations
	Alternative sequence	114 – 298	185	Missing in isoform 4.		VSP_027347
	Alternative sequence	114 – 234	121	Missing in isoform 3.		VSP_027348
	Alternative sequence	114 – 163	50	Missing in isoform 2.		VSP_027349
	Natural variant	368	1	S → R. Corresponds to variant rs168107 [ dbSNP | Ensembl ].		VAR_034673

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (hMCLC-1) [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 08709A80E99F2E15	FASTA	551	62,023
	10 20 30 40 50 60 MLCSLLLCEC LLLVAGYAHD DDWIDPTDML NYDAASGTMR KSQAKYGISG EKDVSPDLSC 70 80 90 100 110 120 ADEISECYHK LDSLTYKIDE CEKKKREDYE SQSNPVFRRY LNKILIEAGK LGLPDENKGD 130 140 150 160 170 180 MHYDAEIILK RETLLEIQKF LNGEDWKPGA LDDALSDILI NFKFHDFETW KWRFEDSFGV 190 200 210 220 230 240 DPYNVLMVLL CLLCIVVLVA TELWTYVRWY TQLRRVLIIS FLFSLGWNWM YLYKLAFAQH 250 260 270 280 290 300 QAEVAKMEPL NNVCAKKMDW TGSIWEWFRS SWTYKDDPCQ KYYELLLVNP IWLVPPTKAL 310 320 330 340 350 360 AVTFTTFVTE PLKHIGKGTG EFIKALMKEI PALLHLPVLI IMALAILSFC YGAGKSVHVL 370 380 390 400 410 420 RHIGGPESEP PQALRPRDRR RQEEIDYRPD GGAGDADFHY RGQMGPTEQG PYAKTYEGRR 430 440 450 460 470 480 EILRERDVDL RFQTGNKSPE VLRAFDVPDA EAREHPTVVP SHKSPVLDTK PKETGGILGE 490 500 510 520 530 540 GTPKESSTES SQSAKPVSGQ DTSGNTEGSP AAEKAQLKSE AAGSPDQGST YSPARGVAGP 550 RGQDPVSSPC G
	Isoform 2 (hMCLC-2) [UniParc]. Checksum: 2AAB4D99DA5EB327	FASTA	501	56,267
	10 20 30 40 50 60 MLCSLLLCEC LLLVAGYAHD DDWIDPTDML NYDAASGTMR KSQAKYGISG EKDVSPDLSC 70 80 90 100 110 120 ADEISECYHK LDSLTYKIDE CEKKKREDYE SQSNPVFRRY LNKILIEAGK LGLFHDFETW 130 140 150 160 170 180 KWRFEDSFGV DPYNVLMVLL CLLCIVVLVA TELWTYVRWY TQLRRVLIIS FLFSLGWNWM 190 200 210 220 230 240 YLYKLAFAQH QAEVAKMEPL NNVCAKKMDW TGSIWEWFRS SWTYKDDPCQ KYYELLLVNP 250 260 270 280 290 300 IWLVPPTKAL AVTFTTFVTE PLKHIGKGTG EFIKALMKEI PALLHLPVLI IMALAILSFC 310 320 330 340 350 360 YGAGKSVHVL RHIGGPESEP PQALRPRDRR RQEEIDYRPD GGAGDADFHY RGQMGPTEQG 370 380 390 400 410 420 PYAKTYEGRR EILRERDVDL RFQTGNKSPE VLRAFDVPDA EAREHPTVVP SHKSPVLDTK 430 440 450 460 470 480 PKETGGILGE GTPKESSTES SQSAKPVSGQ DTSGNTEGSP AAEKAQLKSE AAGSPDQGST 490 500 YSPARGVAGP RGQDPVSSPC G
	Isoform 3 (hMCLC-3) [UniParc]. Checksum: DB73E1B97B4B9660	FASTA	430	47,437
	10 20 30 40 50 60 MLCSLLLCEC LLLVAGYAHD DDWIDPTDML NYDAASGTMR KSQAKYGISG EKDVSPDLSC 70 80 90 100 110 120 ADEISECYHK LDSLTYKIDE CEKKKREDYE SQSNPVFRRY LNKILIEAGK LGLLAFAQHQ 130 140 150 160 170 180 AEVAKMEPLN NVCAKKMDWT GSIWEWFRSS WTYKDDPCQK YYELLLVNPI WLVPPTKALA 190 200 210 220 230 240 VTFTTFVTEP LKHIGKGTGE FIKALMKEIP ALLHLPVLII MALAILSFCY GAGKSVHVLR 250 260 270 280 290 300 HIGGPESEPP QALRPRDRRR QEEIDYRPDG GAGDADFHYR GQMGPTEQGP YAKTYEGRRE 310 320 330 340 350 360 ILRERDVDLR FQTGNKSPEV LRAFDVPDAE AREHPTVVPS HKSPVLDTKP KETGGILGEG 370 380 390 400 410 420 TPKESSTESS QSAKPVSGQD TSGNTEGSPA AEKAQLKSEA AGSPDQGSTY SPARGVAGPR 430 GQDPVSSPCG
	Isoform 4 (hMCLC-4) [UniParc]. Checksum: 6214C106333DBAEC	FASTA	366	39,838
	10 20 30 40 50 60 MLCSLLLCEC LLLVAGYAHD DDWIDPTDML NYDAASGTMR KSQAKYGISG EKDVSPDLSC 70 80 90 100 110 120 ADEISECYHK LDSLTYKIDE CEKKKREDYE SQSNPVFRRY LNKILIEAGK LGLALAVTFT 130 140 150 160 170 180 TFVTEPLKHI GKGTGEFIKA LMKEIPALLH LPVLIIMALA ILSFCYGAGK SVHVLRHIGG 190 200 210 220 230 240 PESEPPQALR PRDRRRQEEI DYRPDGGAGD ADFHYRGQMG PTEQGPYAKT YEGRREILRE 250 260 270 280 290 300 RDVDLRFQTG NKSPEVLRAF DVPDAEAREH PTVVPSHKSP VLDTKPKETG GILGEGTPKE 310 320 330 340 350 360 SSTESSQSAK PVSGQDTSGN TEGSPAAEKA QLKSEAAGSP DQGSTYSPAR GVAGPRGQDP VSSPCG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a novel chloride channel expressed in the endoplasmic reticulum, Golgi apparatus, and nucleus." Nagasawa M., Kanzaki M., Iino Y., Morishita Y., Kojima I. J. Biol. Chem. 276:20413-20418(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4).
[2]	"Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:277-286(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Skin.
[6]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-438, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[7]	"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-438, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-438; SER-464; SER-509; SER-524 AND SER-532, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[9]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-482; SER-524 AND SER-532, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-509, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB052915 mRNA. Translation: BAB59018.1. AB052916 mRNA. Translation: BAB79261.1. AB052917 mRNA. Translation: BAB79262.1. AB052918 mRNA. Translation: BAB79263.1. AB018304 mRNA. Translation: BAA34481.2. AL449266 Genomic DNA. Translation: CAI14363.1. AL449266 Genomic DNA. Translation: CAI14364.1. AL449266 Genomic DNA. Translation: CAI14365.1. AL449266 Genomic DNA. Translation: CAI14366.1. CH471122 Genomic DNA. Translation: EAW56345.1. CH471122 Genomic DNA. Translation: EAW56346.1. BC002939 mRNA. Translation: AAH02939.1.
RefSeq	NP_001041675.1. NM_001048210.2. NP_001265131.1. NM_001278202.1. NP_001265132.1. NM_001278203.1. NP_055942.1. NM_015127.4.
UniGene	Hs.658489.
3D structure databases
ProteinModelPortal	Q96S66.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	116769. 2 interactions.
IntAct	Q96S66. 2 interactions.
STRING	9606.ENSP00000349456.
Protein family/group databases
TCDB	1.A.36.1.1. the intracellular chloride channel (icc) family.
PTM databases
PhosphoSite	Q96S66.
Polymorphism databases
DMDM	74752121.
Proteomic databases
PaxDb	Q96S66.
PRIDE	Q96S66.
Protocols and materials databases
DNASU	23155.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000302500; ENSP00000306552; ENSG00000121940. [Q96S66-3] ENST00000348264; ENSP00000337243; ENSG00000121940. [Q96S66-4] ENST00000356970; ENSP00000349456; ENSG00000121940. [Q96S66-1] ENST00000369968; ENSP00000358985; ENSG00000121940. [Q96S66-4] ENST00000369969; ENSP00000358986; ENSG00000121940. [Q96S66-3] ENST00000369970; ENSP00000358987; ENSG00000121940. [Q96S66-2] ENST00000369971; ENSP00000358988; ENSG00000121940. [Q96S66-1] ENST00000415331; ENSP00000411591; ENSG00000121940. [Q96S66-2]
GeneID	23155.
KEGG	hsa:23155.
UCSC	uc001dwf.2. human. [Q96S66-1] uc001dwg.2. human. [Q96S66-2] uc009wes.2. human. [Q96S66-3] uc009wet.2. human. [Q96S66-4]
Organism-specific databases
CTD	23155.
GeneCards	GC01M109472.
HGNC	HGNC:29675. CLCC1.
HPA	HPA009087. HPA013210.
neXtProt	NX_Q96S66.
PharmGKB	PA142672105.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG44384.
HOGENOM	HOG000231351.
HOVERGEN	HBG060364.
InParanoid	Q96S66.
OMA	SWTYKDD.
OrthoDB	EOG7B8S3V.
PhylomeDB	Q96S66.
TreeFam	TF328890.
Gene expression databases
ArrayExpress	Q96S66.
Bgee	Q96S66.
CleanEx	HS_CLCC1.
Genevestigator	Q96S66.
Family and domain databases
InterPro	IPR009231. Chloride_chnl_CLIC-like. [Graphical view]
Pfam	PF05934. MCLC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GeneWiki	CLCC1.
GenomeRNAi	23155.
NextBio	44493.
PRO	Q96S66.

Entry information

Entry name

CLCC1_HUMAN

Accession

Primary (citable) accession number: Q96S66
Secondary accession number(s): O94861 , Q8WYP8, Q8WYP9, Q9BU25

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	December 1, 2001
Last modified:	April 16, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.