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Protein

ATPase WRNIP1

Gene

WRNIP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a modulator of initiation or reinitiation events during DNA polymerase delta-mediated DNA synthesis. In the presence of ATP, stimulation of DNA polymerase delta-mediated DNA synthesis is decreased.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri17 – 40UBZ-typeAdd BLAST24
Nucleotide bindingi268 – 275ATPSequence analysis8

GO - Molecular functioni

  • ATPase activity Source: UniProtKB
  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: InterPro
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • DNA replication Source: UniProtKB-KW
  • DNA synthesis involved in DNA repair Source: UniProtKB
  • regulation of DNA-dependent DNA replication initiation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

DNA damage, DNA replication

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000124535-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase WRNIP1 (EC:3.6.1.3)
Alternative name(s):
Werner helicase-interacting protein 1
Gene namesi
Name:WRNIP1Imported
Synonyms:WHIPImported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:20876. WRNIP1.

Subcellular locationi

  • Nucleus By similarity

  • Note: Colocalizes with WRN in granular structures in the nucleus.By similarity

GO - Cellular componenti

  • membrane Source: UniProtKB
  • nucleus Source: UniProtKB
  • perinuclear region of cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi294T → A: Loss of ATPase activity. 1 Publication1

Organism-specific databases

DisGeNETi56897.
OpenTargetsiENSG00000124535.
PharmGKBiPA134982239.

Polymorphism and mutation databases

BioMutaiWRNIP1.
DMDMi73622085.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000847851 – 665ATPase WRNIP1Add BLAST665

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei65PhosphoserineCombined sources1
Modified residuei75PhosphoserineCombined sources1
Cross-linki81Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei85PhosphothreonineCombined sources1
Modified residuei91PhosphoserineCombined sources1
Modified residuei92PhosphoserineCombined sources1
Modified residuei116PhosphothreonineCombined sources1
Modified residuei139PhosphoserineCombined sources1
Cross-linki141Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei153PhosphoserineCombined sources1
Cross-linki225Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki301Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki310Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki316Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki322Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki335Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki482Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei534PhosphotyrosineCombined sources1
Modified residuei562PhosphotyrosineCombined sources1
Cross-linki627Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei633N6-acetyllysine; alternateCombined sources1
Cross-linki633Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate1 Publication
Cross-linki636Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Post-translational modificationi

Sumoylated with SUMO1 and SUMO2/3.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ96S55.
MaxQBiQ96S55.
PaxDbiQ96S55.
PeptideAtlasiQ96S55.
PRIDEiQ96S55.

PTM databases

iPTMnetiQ96S55.
PhosphoSitePlusiQ96S55.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSG00000124535.
CleanExiHS_WRNIP1.
GenevisibleiQ96S55. HS.

Organism-specific databases

HPAiHPA031752.
HPA031753.

Interactioni

Subunit structurei

May form homooligomers, possibly octamers. Directly interacts with POLD1, POLD2 and POLD4 (PubMed:15670210). Interacts with the N-terminal domain of WRN (By similarity). Interacts (via UBZ-type zinc finger) with polyubiquitin (PubMed:17550899).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-2513471,EBI-2513471
DDX58O957862EBI-2513471,EBI-995350
POLD1P283402EBI-2513471,EBI-716569
POLD2P490052EBI-2513471,EBI-372354
POLD4Q9HCU82EBI-2513471,EBI-864968
TOLLIPQ9H0E22EBI-2513471,EBI-74615

Protein-protein interaction databases

BioGridi121227. 35 interactors.
IntActiQ96S55. 22 interactors.
MINTiMINT-3058154.
STRINGi9606.ENSP00000370150.

Structurei

Secondary structure

1665
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi17 – 19Combined sources3
Turni21 – 23Combined sources3
Beta strandi26 – 28Combined sources3
Helixi29 – 31Combined sources3
Helixi32 – 39Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3VHSX-ray1.90A/B17-40[»]
3VHTX-ray2.40B9-46[»]
ProteinModelPortaliQ96S55.
SMRiQ96S55.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 UBZ-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri17 – 40UBZ-typeAdd BLAST24

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2028. Eukaryota.
COG2256. LUCA.
GeneTreeiENSGT00390000008538.
HOVERGENiHBG062192.
InParanoidiQ96S55.
KOiK07478.
OMAiPGHWDAD.
OrthoDBiEOG091G09JH.
PhylomeDBiQ96S55.
TreeFamiTF324547.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR032423. AAA_assoc_2.
IPR003959. ATPase_AAA_core.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR021886. MgsA_C.
IPR027417. P-loop_NTPase.
IPR006642. Znf_Rad18_put.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF16193. AAA_assoc_2. 1 hit.
PF12002. MgsA_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00734. ZnF_Rad18. 1 hit.
[Graphical view]
SUPFAMiSSF48019. SSF48019. 1 hit.
SSF52540. SSF52540. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q96S55-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEVSGPEDDP FLSQLHQVQC PVCQQMMPAA HINSHLDRCL LLHPAGHAEP
60 70 80 90 100
AAGSHRAGER AKGPSPPGAK RRRLSESSAL KQPATPTAAE SSEGEGEEGD
110 120 130 140 150
DGGETESRES YDAPPTPSGA RLIPDFPVAR SSSPGRKGSG KRPAAAAAAG
160 170 180 190 200
SASPRSWDEA EAQEEEEAVG DGDGDGDADA DGEDDPGHWD ADAAEAATAF
210 220 230 240 250
GASGGGRPHP RALAAEEIRQ MLQGKPLADT MRPDTLQDYF GQSKAVGQDT
260 270 280 290 300
LLRSLLETNE IPSLILWGPP GCGKTTLAHI IASNSKKHSI RFVTLSATNA
310 320 330 340 350
KTNDVRDVIK QAQNEKSFFK RKTILFIDEI HRFNKSQQDT FLPHVECGTI
360 370 380 390 400
TLIGATTENP SFQVNAALLS RCRVIVLEKL PVEAMVTILM RAINSLGIHV
410 420 430 440 450
LDSSRPTDPL SHSSNSSSEP AMFIEDKAVD TLAYLSDGDA RAGLNGLQLA
460 470 480 490 500
VLARLSSRKM FCKKSGQSYS PSRVLITEND VKEGLQRSHI LYDRAGEEHY
510 520 530 540 550
NCISALHKSM RGSDQNASLY WLARMLEGGE DPLYVARRLV RFASEDIGLA
560 570 580 590 600
DPSALTQAVA AYQGCHFIGM PECEVLLAQC VVYFARAPKS IEVYSAYNNV
610 620 630 640 650
KACLRNHQGP LPPVPLHLRN APTRLMKDLG YGKGYKYNPM YSEPVDQEYL
660
PEELRGVDFF KQRRC
Length:665
Mass (Da):72,133
Last modified:August 16, 2005 - v2
Checksum:iDE43D8E59C4B29E8
GO
Isoform 21 Publication (identifier: Q96S55-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     338-362: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:640
Mass (Da):69,459
Checksum:i83117E6CEFDE7688
GO
Isoform 31 Publication (identifier: Q96S55-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-220: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:445
Mass (Da):49,570
Checksum:iBDED370A47C0D894
GO
Isoform 41 Publication (identifier: Q96S55-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-384: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:281
Mass (Da):31,339
Checksum:i1099DF56AA75F54A
GO

Sequence cautioni

The sequence AAF80563 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB15383 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti144A → V in BAB60709 (PubMed:11301316).Curated1
Sequence conflicti170 – 173Missing in BAD92960 (Ref. 3) Curated4
Sequence conflicti265I → N in BAB60709 (PubMed:11301316).Curated1
Sequence conflicti377L → F in BAB15383 (PubMed:14702039).Curated1
Sequence conflicti499H → Y in BAD97313 (Ref. 3) Curated1
Sequence conflicti545E → G in BAB15383 (PubMed:14702039).Curated1
Sequence conflicti568I → M in BAB60709 (PubMed:11301316).Curated1
Sequence conflicti576L → F in BAB60709 (PubMed:11301316).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0517821 – 384Missing in isoform 4. 1 PublicationAdd BLAST384
Alternative sequenceiVSP_0517811 – 220Missing in isoform 3. 1 PublicationAdd BLAST220
Alternative sequenceiVSP_051783338 – 362Missing in isoform 2. 3 PublicationsAdd BLAST25

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056152 mRNA. Translation: BAB60709.1.
AK026179 mRNA. Translation: BAB15383.1. Different initiation.
AK315471 mRNA. Translation: BAG37857.1.
AB209723 mRNA. Translation: BAD92960.1.
AK223593 mRNA. Translation: BAD97313.1.
AL139092 Genomic DNA. Translation: CAH73663.1.
AL139092 Genomic DNA. Translation: CAH73664.1.
AL139092 Genomic DNA. Translation: CAH73665.1.
AL139092 Genomic DNA. Translation: CAH73666.1.
CH471087 Genomic DNA. Translation: EAW55087.1.
BC018923 mRNA. Translation: AAH18923.1.
AF218313 mRNA. Translation: AAF80563.1. Different initiation.
CCDSiCCDS4475.1. [Q96S55-1]
CCDS4476.1. [Q96S55-2]
RefSeqiNP_064520.2. NM_020135.2. [Q96S55-1]
NP_569079.1. NM_130395.2. [Q96S55-2]
UniGeneiHs.236828.

Genome annotation databases

EnsembliENST00000380764; ENSP00000370141; ENSG00000124535. [Q96S55-4]
ENST00000380769; ENSP00000370146; ENSG00000124535. [Q96S55-3]
ENST00000380771; ENSP00000370148; ENSG00000124535. [Q96S55-2]
ENST00000380773; ENSP00000370150; ENSG00000124535. [Q96S55-1]
ENST00000618555; ENSP00000477551; ENSG00000124535. [Q96S55-1]
GeneIDi56897.
KEGGihsa:56897.
UCSCiuc003mtz.4. human. [Q96S55-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056152 mRNA. Translation: BAB60709.1.
AK026179 mRNA. Translation: BAB15383.1. Different initiation.
AK315471 mRNA. Translation: BAG37857.1.
AB209723 mRNA. Translation: BAD92960.1.
AK223593 mRNA. Translation: BAD97313.1.
AL139092 Genomic DNA. Translation: CAH73663.1.
AL139092 Genomic DNA. Translation: CAH73664.1.
AL139092 Genomic DNA. Translation: CAH73665.1.
AL139092 Genomic DNA. Translation: CAH73666.1.
CH471087 Genomic DNA. Translation: EAW55087.1.
BC018923 mRNA. Translation: AAH18923.1.
AF218313 mRNA. Translation: AAF80563.1. Different initiation.
CCDSiCCDS4475.1. [Q96S55-1]
CCDS4476.1. [Q96S55-2]
RefSeqiNP_064520.2. NM_020135.2. [Q96S55-1]
NP_569079.1. NM_130395.2. [Q96S55-2]
UniGeneiHs.236828.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3VHSX-ray1.90A/B17-40[»]
3VHTX-ray2.40B9-46[»]
ProteinModelPortaliQ96S55.
SMRiQ96S55.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121227. 35 interactors.
IntActiQ96S55. 22 interactors.
MINTiMINT-3058154.
STRINGi9606.ENSP00000370150.

PTM databases

iPTMnetiQ96S55.
PhosphoSitePlusiQ96S55.

Polymorphism and mutation databases

BioMutaiWRNIP1.
DMDMi73622085.

Proteomic databases

EPDiQ96S55.
MaxQBiQ96S55.
PaxDbiQ96S55.
PeptideAtlasiQ96S55.
PRIDEiQ96S55.

Protocols and materials databases

DNASUi56897.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000380764; ENSP00000370141; ENSG00000124535. [Q96S55-4]
ENST00000380769; ENSP00000370146; ENSG00000124535. [Q96S55-3]
ENST00000380771; ENSP00000370148; ENSG00000124535. [Q96S55-2]
ENST00000380773; ENSP00000370150; ENSG00000124535. [Q96S55-1]
ENST00000618555; ENSP00000477551; ENSG00000124535. [Q96S55-1]
GeneIDi56897.
KEGGihsa:56897.
UCSCiuc003mtz.4. human. [Q96S55-1]

Organism-specific databases

CTDi56897.
DisGeNETi56897.
GeneCardsiWRNIP1.
H-InvDBHIX0005529.
HGNCiHGNC:20876. WRNIP1.
HPAiHPA031752.
HPA031753.
MIMi608196. gene.
neXtProtiNX_Q96S55.
OpenTargetsiENSG00000124535.
PharmGKBiPA134982239.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2028. Eukaryota.
COG2256. LUCA.
GeneTreeiENSGT00390000008538.
HOVERGENiHBG062192.
InParanoidiQ96S55.
KOiK07478.
OMAiPGHWDAD.
OrthoDBiEOG091G09JH.
PhylomeDBiQ96S55.
TreeFamiTF324547.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000124535-MONOMER.

Miscellaneous databases

GeneWikiiWRNIP1.
GenomeRNAii56897.
PROiQ96S55.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000124535.
CleanExiHS_WRNIP1.
GenevisibleiQ96S55. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR032423. AAA_assoc_2.
IPR003959. ATPase_AAA_core.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR021886. MgsA_C.
IPR027417. P-loop_NTPase.
IPR006642. Znf_Rad18_put.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF16193. AAA_assoc_2. 1 hit.
PF12002. MgsA_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00734. ZnF_Rad18. 1 hit.
[Graphical view]
SUPFAMiSSF48019. SSF48019. 1 hit.
SSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiWRIP1_HUMAN
AccessioniPrimary (citable) accession number: Q96S55
Secondary accession number(s): B2RDB0
, Q53EP6, Q59ET8, Q5W0E2, Q5W0E4, Q8WV26, Q9H681, Q9NRJ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: August 16, 2005
Last modified: November 30, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.