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Protein

ATPase WRNIP1

Gene

WRNIP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as a modulator of initiation or reinitiation events during DNA polymerase delta-mediated DNA synthesis. In the presence of ATP, stimulation of DNA polymerase delta-mediated DNA synthesis is decreased. Plays also a role in the innate immune defense against viruses. Stabilizes the RIG-I/DDX58 dsRNA interaction and promotes RIG-I/DDX58 'Lys-63'-linked polyubiquitination. In turn, RIG-I/DDX58 transmits the signal through mitochondrial MAVS.2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi20ZincCombined sources1 Publication1
Metal bindingi23ZincCombined sources1 Publication1
Metal bindingi31Zinc; via tele nitrogenCombined sources1 Publication1
Metal bindingi35Zinc; via tele nitrogenCombined sources1 Publication1
Metal bindingi39ZincCombined sources1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri17 – 40UBZ-typeSequence analysisAdd BLAST24
Nucleotide bindingi270 – 276ATPBy similarity7

GO - Molecular functioni

  • ATPase activity Source: UniProtKB
  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: InterPro
  • identical protein binding Source: IntAct
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • DNA replication Source: UniProtKB-KW
  • DNA synthesis involved in DNA repair Source: UniProtKB
  • innate immune response Source: UniProtKB-KW
  • regulation of DNA-dependent DNA replication initiation Source: UniProtKB

Keywordsi

Molecular functionHydrolase
Biological processDNA damage, DNA replication, Immunity, Innate immunity
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase WRNIP1 (EC:3.6.1.31 Publication)
Alternative name(s):
Werner helicase-interacting protein 1
Gene namesi
Name:WRNIP1Imported
Synonyms:WHIPImported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000124535.15
HGNCiHGNC:20876 WRNIP1
MIMi608196 gene
neXtProtiNX_Q96S55

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi18V → A: Reduced affinity for ubiquitin. 1 Publication1
Mutagenesisi21P → A: Loss of affinity for ubiquitin. 1 Publication1
Mutagenesisi22V → A: Reduced affinity for ubiquitin. 1 Publication1
Mutagenesisi32I → A: Loss of affinity for ubiquitin. 1 Publication1
Mutagenesisi33N → A: Loss of affinity for ubiquitin. 1 Publication1
Mutagenesisi36L → A: Loss of affinity for ubiquitin. 1 Publication1
Mutagenesisi37D → A: Loss of affinity for ubiquitin. 1 Publication1
Mutagenesisi40L → A: Loss of affinity for ubiquitin. 1 Publication1
Mutagenesisi41L → A: Normal affinity for ubiquitin. 1 Publication1
Mutagenesisi42L → A: Normal affinity for ubiquitin. 1 Publication1
Mutagenesisi44P → A: Normal affinity for ubiquitin. 1 Publication1
Mutagenesisi294T → A: Loss of ATPase activity. 1 Publication1

Organism-specific databases

DisGeNETi56897
OpenTargetsiENSG00000124535
PharmGKBiPA134982239

Polymorphism and mutation databases

BioMutaiWRNIP1
DMDMi73622085

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000847851 – 665ATPase WRNIP1Add BLAST665

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei65PhosphoserineCombined sources1
Modified residuei75PhosphoserineCombined sources1
Cross-linki81Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei85PhosphothreonineCombined sources1
Modified residuei91PhosphoserineCombined sources1
Modified residuei92PhosphoserineCombined sources1
Modified residuei116PhosphothreonineCombined sources1
Modified residuei139PhosphoserineCombined sources1
Cross-linki141Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei153PhosphoserineCombined sources1
Cross-linki225Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki301Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki310Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki316Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki322Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki335Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki482Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Cross-linki482Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate1 Publication
Modified residuei534PhosphotyrosineCombined sources1
Modified residuei562PhosphotyrosineCombined sources1
Cross-linki627Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei633N6-acetyllysine; alternateCombined sources1
Cross-linki633Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate1 Publication
Cross-linki636Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Post-translational modificationi

Sumoylated with SUMO1 and SUMO2/3.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ96S55
MaxQBiQ96S55
PaxDbiQ96S55
PeptideAtlasiQ96S55
PRIDEiQ96S55

PTM databases

iPTMnetiQ96S55
PhosphoSitePlusiQ96S55

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSG00000124535
CleanExiHS_WRNIP1
GenevisibleiQ96S55 HS

Organism-specific databases

HPAiHPA031752
HPA031753

Interactioni

Subunit structurei

Forms homooligomers (PubMed:18842586), possibly octamers. Directly interacts with POLD1, POLD2 and POLD4 (PubMed:15670210). Interacts with the N-terminal domain of WRN (By similarity). Interacts (via UBZ-type zinc finger) with monoubiquitin and polyubiquitin (PubMed:17550899, PubMed:18842586). Interacts with TRIM14 and PPP6C; these interactions positively regulate the RIG-I/DDX58 signaling pathway (PubMed:29053956).By similarity4 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi121227, 36 interactors
IntActiQ96S55, 23 interactors
MINTiQ96S55
STRINGi9606.ENSP00000370150

Structurei

Secondary structure

1665
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi17 – 19Combined sources3
Turni21 – 23Combined sources3
Beta strandi26 – 28Combined sources3
Helixi29 – 31Combined sources3
Helixi32 – 39Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3VHSX-ray1.90A/B17-40[»]
3VHTX-ray2.40B9-46[»]
ProteinModelPortaliQ96S55
SMRiQ96S55
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi144 – 215Ala-richPROSITE-ProRule annotationAdd BLAST72

Domaini

The UBZ-type zinc finger binds ubiquitin.1 Publication

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri17 – 40UBZ-typeSequence analysisAdd BLAST24

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2028 Eukaryota
COG2256 LUCA
GeneTreeiENSGT00390000008538
HOVERGENiHBG062192
InParanoidiQ96S55
KOiK07478
OMAiRIILSQC
OrthoDBiEOG091G09JH
PhylomeDBiQ96S55
TreeFamiTF324547

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR032423 AAA_assoc_2
IPR003959 ATPase_AAA_core
IPR008921 DNA_pol3_clamp-load_cplx_C
IPR021886 MgsA_C
IPR027417 P-loop_NTPase
IPR006642 Znf_Rad18_put
PfamiView protein in Pfam
PF00004 AAA, 1 hit
PF16193 AAA_assoc_2, 1 hit
PF12002 MgsA_C, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00734 ZnF_Rad18, 1 hit
SUPFAMiSSF48019 SSF48019, 1 hit
SSF52540 SSF52540, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q96S55-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEVSGPEDDP FLSQLHQVQC PVCQQMMPAA HINSHLDRCL LLHPAGHAEP
60 70 80 90 100
AAGSHRAGER AKGPSPPGAK RRRLSESSAL KQPATPTAAE SSEGEGEEGD
110 120 130 140 150
DGGETESRES YDAPPTPSGA RLIPDFPVAR SSSPGRKGSG KRPAAAAAAG
160 170 180 190 200
SASPRSWDEA EAQEEEEAVG DGDGDGDADA DGEDDPGHWD ADAAEAATAF
210 220 230 240 250
GASGGGRPHP RALAAEEIRQ MLQGKPLADT MRPDTLQDYF GQSKAVGQDT
260 270 280 290 300
LLRSLLETNE IPSLILWGPP GCGKTTLAHI IASNSKKHSI RFVTLSATNA
310 320 330 340 350
KTNDVRDVIK QAQNEKSFFK RKTILFIDEI HRFNKSQQDT FLPHVECGTI
360 370 380 390 400
TLIGATTENP SFQVNAALLS RCRVIVLEKL PVEAMVTILM RAINSLGIHV
410 420 430 440 450
LDSSRPTDPL SHSSNSSSEP AMFIEDKAVD TLAYLSDGDA RAGLNGLQLA
460 470 480 490 500
VLARLSSRKM FCKKSGQSYS PSRVLITEND VKEGLQRSHI LYDRAGEEHY
510 520 530 540 550
NCISALHKSM RGSDQNASLY WLARMLEGGE DPLYVARRLV RFASEDIGLA
560 570 580 590 600
DPSALTQAVA AYQGCHFIGM PECEVLLAQC VVYFARAPKS IEVYSAYNNV
610 620 630 640 650
KACLRNHQGP LPPVPLHLRN APTRLMKDLG YGKGYKYNPM YSEPVDQEYL
660
PEELRGVDFF KQRRC
Length:665
Mass (Da):72,133
Last modified:August 16, 2005 - v2
Checksum:iDE43D8E59C4B29E8
GO
Isoform 21 Publication (identifier: Q96S55-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     338-362: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:640
Mass (Da):69,459
Checksum:i83117E6CEFDE7688
GO
Isoform 31 Publication (identifier: Q96S55-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-220: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:445
Mass (Da):49,570
Checksum:iBDED370A47C0D894
GO
Isoform 41 Publication (identifier: Q96S55-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-384: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:281
Mass (Da):31,339
Checksum:i1099DF56AA75F54A
GO

Sequence cautioni

The sequence AAF80563 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB15383 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti144A → V in BAB60709 (PubMed:11301316).Curated1
Sequence conflicti170 – 173Missing in BAD92960 (Ref. 3) Curated4
Sequence conflicti265I → N in BAB60709 (PubMed:11301316).Curated1
Sequence conflicti377L → F in BAB15383 (PubMed:14702039).Curated1
Sequence conflicti499H → Y in BAD97313 (Ref. 3) Curated1
Sequence conflicti545E → G in BAB15383 (PubMed:14702039).Curated1
Sequence conflicti568I → M in BAB60709 (PubMed:11301316).Curated1
Sequence conflicti576L → F in BAB60709 (PubMed:11301316).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0517821 – 384Missing in isoform 4. 1 PublicationAdd BLAST384
Alternative sequenceiVSP_0517811 – 220Missing in isoform 3. 1 PublicationAdd BLAST220
Alternative sequenceiVSP_051783338 – 362Missing in isoform 2. 3 PublicationsAdd BLAST25

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056152 mRNA Translation: BAB60709.1
AK026179 mRNA Translation: BAB15383.1 Different initiation.
AK315471 mRNA Translation: BAG37857.1
AB209723 mRNA Translation: BAD92960.1
AK223593 mRNA Translation: BAD97313.1
AL139092 Genomic DNA Translation: CAH73663.1
AL139092 Genomic DNA Translation: CAH73664.1
AL139092 Genomic DNA Translation: CAH73665.1
AL139092 Genomic DNA Translation: CAH73666.1
CH471087 Genomic DNA Translation: EAW55087.1
BC018923 mRNA Translation: AAH18923.1
AF218313 mRNA Translation: AAF80563.1 Different initiation.
CCDSiCCDS4475.1 [Q96S55-1]
CCDS4476.1 [Q96S55-2]
RefSeqiNP_064520.2, NM_020135.2 [Q96S55-1]
NP_569079.1, NM_130395.2 [Q96S55-2]
UniGeneiHs.236828

Genome annotation databases

EnsembliENST00000380764; ENSP00000370141; ENSG00000124535 [Q96S55-4]
ENST00000380769; ENSP00000370146; ENSG00000124535 [Q96S55-3]
ENST00000380771; ENSP00000370148; ENSG00000124535 [Q96S55-2]
ENST00000380773; ENSP00000370150; ENSG00000124535 [Q96S55-1]
ENST00000618555; ENSP00000477551; ENSG00000124535 [Q96S55-1]
GeneIDi56897
KEGGihsa:56897
UCSCiuc003mtz.4 human [Q96S55-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiWRIP1_HUMAN
AccessioniPrimary (citable) accession number: Q96S55
Secondary accession number(s): B2RDB0
, Q53EP6, Q59ET8, Q5W0E2, Q5W0E4, Q8WV26, Q9H681, Q9NRJ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: August 16, 2005
Last modified: May 23, 2018
This is version 143 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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