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Q96S53 (TESK2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dual specificity testis-specific protein kinase 2

EC=2.7.12.1
Alternative name(s):
Testicular protein kinase 2
Gene names
Name:TESK2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length571 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation of exogenous substrates on both serine/threonine and tyrosine residues. Phosphorylates cofilin at 'Ser-3'. May play an important role in spermatogenesis.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium By similarity.

Manganese By similarity.

Enzyme regulation

Activated by autophosphorylation on Ser-219 By similarity.

Subcellular location

Nucleus Ref.2.

Tissue specificity

Predominantly expressed in testis and prostate. Found predominantly in non-germinal Sertoli cells. Ref.1 Ref.2

Sequence similarities

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

HSP90AB1P082382EBI-1384110,EBI-352572

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Experimental confirmation may be lacking for some isoforms.
Isoform 1 (identifier: Q96S53-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96S53-2)

The sequence of this isoform differs from the canonical sequence as follows:
     116-131: Missing.
Isoform 3 (identifier: Q96S53-3)

The sequence of this isoform differs from the canonical sequence as follows:
     265-293: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 571571Dual specificity testis-specific protein kinase 2
PRO_0000086749

Regions

Domain58 – 313256Protein kinase
Nucleotide binding64 – 729ATP By similarity

Sites

Active site1761Proton acceptor By similarity
Binding site871ATP By similarity

Amino acid modifications

Modified residue2191Phosphoserine; by autocatalysis By similarity

Natural variations

Alternative sequence116 – 13116Missing in isoform 2.
VSP_004930
Alternative sequence265 – 29329Missing in isoform 3.
VSP_004931
Natural variant111G → A in a breast infiltrating ductal carcinoma sample; somatic mutation. Ref.7
VAR_041214

Experimental info

Sequence conflict4551R → C in AAH33085. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: D83B0E58321A46FA

FASTA57163,639
        10         20         30         40         50         60 
MDRSKRNSIA GFPPRVERLE EFEGGGGGEG NVSQVGRVWP SSYRALISAF SRLTRLDDFT 

        70         80         90        100        110        120 
CEKIGSGFFS EVFKVRHRAS GQVMALKMNT LSSNRANMLK EVQLMNRLSH PNILRFMGVC 

       130        140        150        160        170        180 
VHQGQLHALT EYINSGNLEQ LLDSNLHLPW TVRVKLAYDI AVGLSYLHFK GIFHRDLTSK 

       190        200        210        220        230        240 
NCLIKRDENG YSAVVADFGL AEKIPDVSMG SEKLAVVGSP FWMAPEVLRD EPYNEKADVF 

       250        260        270        280        290        300 
SYGIILCEII ARIQADPDYL PRTENFGLDY DAFQHMVGDC PPDFLQLTFN CCNMDPKLRP 

       310        320        330        340        350        360 
SFVEIGKTLE EILSRLQEEE QERDRKLQPT ARGLLEKAPG VKRLSSLDDK IPHKSPCPRR 

       370        380        390        400        410        420 
TIWLSRSQSD IFSRKPPRTV SVLDPYYRPR DGAARTPKVN PFSARQDLMG GKIKFFDLPS 

       430        440        450        460        470        480 
KSVISLVFDL DAPGPGTMPL ADWQEPLAPP IRRWRSLPGS PEFLHQEACP FVGREESLSD 

       490        500        510        520        530        540 
GPPPRLSSLK YRVKEIPPFR ASALPAAQAH EAMDCSILQE ENGFGSRPQG TSPCPAGASE 

       550        560        570 
EMEVEERPAG STPATFSTSG IGLQTQGKQD G 

« Hide

Isoform 2 [UniParc].

Checksum: EFA1A38A53B68FD9
Show »

FASTA55561,887
Isoform 3 [UniParc].

Checksum: A4BE7D25116F20E1
Show »

FASTA54260,332

References

« Hide 'large scale' references
[1]"Identification and characterization of TESK2, a novel member of the LIMK/TESK family of protein kinases, predominantly expressed in testis."
Rosok O., Pedeutour F., Ree A.H., Aasheim H.-C.
Genomics 61:44-54(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY.
Tissue: Testis.
[2]"Cofilin phosphorylation and actin reorganization activities of testicular protein kinase 2 and its predominant expression in testicular Sertoli cells."
Toshima J., Toshima J.Y., Takeuchi K., Mori R., Mizuno K.
J. Biol. Chem. 276:31449-31458(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Brain.
[7]"Patterns of somatic mutation in human cancer genomes."
Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. expand/collapse author list , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] ALA-11.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ132545 mRNA. Translation: CAB41970.1.
AB057597 mRNA. Translation: BAB62909.1.
AK027573 mRNA. Translation: BAG51348.1.
AL451136, AL359540 Genomic DNA. Translation: CAI13092.1.
AL451136, AL359540 Genomic DNA. Translation: CAI13093.1.
AL359540, AL451136 Genomic DNA. Translation: CAI21723.1.
AL359540, AL451136 Genomic DNA. Translation: CAI21724.1.
CH471059 Genomic DNA. Translation: EAX06987.1.
BC033085 mRNA. Translation: AAH33085.1.
RefSeqNP_009101.2. NM_007170.2.
UniGeneHs.591499.

3D structure databases

ProteinModelPortalQ96S53.
SMRQ96S53. Positions 48-363.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115689. 2 interactions.
IntActQ96S53. 1 interaction.
STRING9606.ENSP00000361158.

Chemistry

ChEMBLCHEMBL2069163.

PTM databases

PhosphoSiteQ96S53.

Polymorphism databases

DMDM25009462.

Proteomic databases

PaxDbQ96S53.
PRIDEQ96S53.

Protocols and materials databases

DNASU10420.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000341771; ENSP00000343940; ENSG00000070759. [Q96S53-3]
ENST00000372084; ENSP00000361156; ENSG00000070759. [Q96S53-3]
ENST00000372086; ENSP00000361158; ENSG00000070759. [Q96S53-1]
GeneID10420.
KEGGhsa:10420.
UCSCuc001cns.1. human. [Q96S53-1]
uc009vxr.1. human. [Q96S53-3]

Organism-specific databases

CTD10420.
GeneCardsGC01M045809.
HGNCHGNC:11732. TESK2.
HPAHPA027257.
MIM604746. gene.
neXtProtNX_Q96S53.
PharmGKBPA36449.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000231415.
HOVERGENHBG058204.
InParanoidQ96S53.
KOK08842.
OMANVLDPYY.
OrthoDBEOG7V7664.
PhylomeDBQ96S53.
TreeFamTF318014.

Enzyme and pathway databases

BRENDA2.7.10.2. 2681.

Gene expression databases

ArrayExpressQ96S53.
BgeeQ96S53.
CleanExHS_TESK2.
GenevestigatorQ96S53.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSPR00109. TYRKINASE.
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTESK2. human.
GeneWikiTESK2.
GenomeRNAi10420.
NextBio39492.
PROQ96S53.
SOURCESearch...

Entry information

Entry nameTESK2_HUMAN
AccessionPrimary (citable) accession number: Q96S53
Secondary accession number(s): Q5T422 expand/collapse secondary AC list , Q5T423, Q8N520, Q9Y3Q6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: December 1, 2001
Last modified: April 16, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM