Reviewed,
UniProtKB/Swiss-Prot Q96S53 (TESK2_HUMAN)
Last modified
November 3, 2009.
Version 82.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
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Names and origin
| Protein names | Recommended name: Dual specificity testis-specific protein kinase 2 EC=2.7.12.1 Alternative name(s): Testicular protein kinase 2 | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) [Complete proteome] | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 571 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation of exogenous substrates on both serine/threonine and tyrosine residues. Phosphorylates cofilin at 'Ser-3'. May play an important role in spermatogenesis. |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Cofactor | Magnesium By similarity. Manganese By similarity. |
| Enzyme regulation | Activated by autophosphorylation on Ser-219 By similarity. |
| Subcellular location | |
| Tissue specificity | Predominantly expressed in testis and prostate. Found predominantly in non-germinal Sertoli cells. Ref.2 Ref.1 |
| Sequence similarities | Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Contains 1 protein kinase domain. |
Ontologies
Alternative products
| This entry describes 3 isoforms produced by alternative splicing. [Align] [Select] Note: Experimental confirmation may be lacking for some isoforms. | ||||||
| Isoform 1 (identifier: Q96S53-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q96S53-2) The sequence of this isoform differs from the canonical sequence as follows: 116-131: Missing. | ||||||
| Isoform 3 (identifier: Q96S53-3) The sequence of this isoform differs from the canonical sequence as follows: 265-293: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 571 | 571 | Dual specificity testis-specific protein kinase 2 | PRO_0000086749 | |||||
Regions | |||||||||
| Domain | 58 – 313 | 256 | Protein kinase | ||||||
| Nucleotide binding | 64 – 72 | 9 | ATP By similarity | ||||||
Sites | |||||||||
| Active site | 176 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 87 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 219 | 1 | Phosphoserine; by autocatalysis By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 116 – 131 | 16 | Missing in isoform 2. | VSP_004930 | |||||
| Alternative sequence | 265 – 293 | 29 | Missing in isoform 3. | VSP_004931 | |||||
| Natural variant | 11 | 1 | G → A in a breast infiltrating ductal carcinoma sample; somatic mutation. Ref.4 | VAR_041214 | |||||
Experimental info | |||||||||
| Sequence conflict | 455 | 1 | R → C in AAH33085. Ref.3 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Identification and characterization of TESK2, a novel member of the LIMK/TESK family of protein kinases, predominantly expressed in testis." Rosok O., Pedeutour F., Ree A.H., Aasheim H.-C. Genomics 61:44-54(1999) [PubMed: 10512679] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY. Tissue: Testis. |
| [2] | "Cofilin phosphorylation and actin reorganization activities of testicular protein kinase 2 and its predominant expression in testicular Sertoli cells." Toshima J., Toshima J.Y., Takeuchi K., Mori R., Mizuno K. J. Biol. Chem. 276:31449-31458(2001) [PubMed: 11418599] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, SUBCELLULAR LOCATION. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Brain. |
| [4] | "Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. Stratton M.R.Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] ALA-11. |
Cross-references
Sequence databases | |
|---|---|
| AJ132545 mRNA. Translation: CAB41970.1. AB057597 mRNA. Translation: BAB62909.1. BC033085 mRNA. Translation: AAH33085.1. | |
| IPI | IPI00102677. IPI00217726. IPI00217728. |
| RefSeq | NP_009101.2. |
| UniGene | Hs.271353 Hs.591499 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1FVR based on UniProtKB Q02763. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q96S53. |
PTM databases | |
| PhosphoSite | Q96S53. |
Proteomic databases | |
| PRIDE | Q96S53. |
Genome annotation databases | |
| Ensembl | ENST00000341771; ENSP00000343940; ENSG00000070759; Homo sapiens. [Genome view] ENST00000372083; ENSP00000361155; ENSG00000070759; Homo sapiens. [Genome view] ENST00000372084; ENSP00000361156; ENSG00000070759; Homo sapiens. [Genome view] ENST00000372086; ENSP00000361158; ENSG00000070759; Homo sapiens. [Genome view] ENST00000451835; ENSP00000397244; ENSG00000070759; Homo sapiens. [Genome view] |
| GeneID | 10420. |
| KEGG | hsa:10420. |
| UCSC | uc001cns.1. human. uc009vxr.1. human. |
Organism-specific databases | |
| CTD | 10420. |
| GeneCards | GC01M045582. |
| H-InvDB | HIX0000530. |
| HGNC | HGNC:11732. TESK2. |
| MIM | 604746. gene. |
| PharmGKB | PA36449. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | Q96S53. |
| HOVERGEN | Q96S53. |
| OMA | RRWRSLP. |
Enzyme and pathway databases | |
| BRENDA | 2.7.10.2. 247. 2.7.12.1. 247. |
Gene expression databases | |
| ArrayExpress | Q96S53. |
| Bgee | Q96S53. |
| CleanEx | HS_TESK2. |
| Genevestigator | Q96S53. |
| GermOnline | ENSG00000070759. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR001245. Tyr_pkinase. IPR008266. Tyr_pkinase_AS. [Graphical view] |
| Pfam | PF00069. Pkinase. 1 hit. [Graphical view] |
| PRINTS | PR00109. TYRKINASE. |
| ProDom | PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| PROSITE | PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 39492. |
| SOURCE | Search... |
Entry information
| Entry name | TESK2_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q96S53 Secondary accession number(s): Q8N520, Q9Y3Q6 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 1 Human chromosome 1: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| Human and mouse protein kinases Human and mouse protein kinases: classification and index |
| SIMILARITY comments Index of protein domains and families |

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