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Protein

Dual specificity testis-specific protein kinase 2

Gene

TESK2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation of exogenous substrates on both serine/threonine and tyrosine residues. Phosphorylates cofilin at 'Ser-3'. May play an important role in spermatogenesis.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Protein has several cofactor binding sites:

Enzyme regulationi

Activated by autophosphorylation on Ser-219.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei87ATPPROSITE-ProRule annotation1
Active sitei176Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi64 – 72ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2 2681
SIGNORiQ96S53

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity testis-specific protein kinase 2 (EC:2.7.12.1)
Alternative name(s):
Testicular protein kinase 2
Gene namesi
Name:TESK2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000070759.16
HGNCiHGNC:11732 TESK2
MIMi604746 gene
neXtProtiNX_Q96S53

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi10420
OpenTargetsiENSG00000070759
PharmGKBiPA36449

Chemistry databases

ChEMBLiCHEMBL2069163
GuidetoPHARMACOLOGYi2240

Polymorphism and mutation databases

BioMutaiTESK2
DMDMi25009462

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000867491 – 571Dual specificity testis-specific protein kinase 2Add BLAST571

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei219Phosphoserine; by autocatalysisBy similarity1
Modified residuei369PhosphoserineCombined sources1
Modified residuei456PhosphoserineCombined sources1
Modified residuei460PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ96S53
PeptideAtlasiQ96S53
PRIDEiQ96S53

PTM databases

iPTMnetiQ96S53
PhosphoSitePlusiQ96S53

Expressioni

Tissue specificityi

Predominantly expressed in testis and prostate. Found predominantly in non-germinal Sertoli cells.2 Publications

Gene expression databases

BgeeiENSG00000070759
CleanExiHS_TESK2
GenevisibleiQ96S53 HS

Organism-specific databases

HPAiHPA027257
HPA063869

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
HSP90AB1P082382EBI-1384110,EBI-352572

Protein-protein interaction databases

BioGridi115689, 68 interactors
IntActiQ96S53, 6 interactors
STRINGi9606.ENSP00000361158

Chemistry databases

BindingDBiQ96S53

Structurei

3D structure databases

ProteinModelPortaliQ96S53
SMRiQ96S53
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini58 – 313Protein kinasePROSITE-ProRule annotationAdd BLAST256

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IF1S Eukaryota
COG0515 LUCA
GeneTreeiENSGT00530000063025
HOGENOMiHOG000231415
HOVERGENiHBG058204
InParanoidiQ96S53
KOiK08842
OMAiNVLDPYY
OrthoDBiEOG091G0HLL
PhylomeDBiQ96S53
TreeFamiTF318014

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR008266 Tyr_kinase_AS
PfamiView protein in Pfam
PF07714 Pkinase_Tyr, 1 hit
PRINTSiPR00109 TYRKINASE
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00109 PROTEIN_KINASE_TYR, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Experimental confirmation may be lacking for some isoforms.
Isoform 1 (identifier: Q96S53-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDRSKRNSIA GFPPRVERLE EFEGGGGGEG NVSQVGRVWP SSYRALISAF
60 70 80 90 100
SRLTRLDDFT CEKIGSGFFS EVFKVRHRAS GQVMALKMNT LSSNRANMLK
110 120 130 140 150
EVQLMNRLSH PNILRFMGVC VHQGQLHALT EYINSGNLEQ LLDSNLHLPW
160 170 180 190 200
TVRVKLAYDI AVGLSYLHFK GIFHRDLTSK NCLIKRDENG YSAVVADFGL
210 220 230 240 250
AEKIPDVSMG SEKLAVVGSP FWMAPEVLRD EPYNEKADVF SYGIILCEII
260 270 280 290 300
ARIQADPDYL PRTENFGLDY DAFQHMVGDC PPDFLQLTFN CCNMDPKLRP
310 320 330 340 350
SFVEIGKTLE EILSRLQEEE QERDRKLQPT ARGLLEKAPG VKRLSSLDDK
360 370 380 390 400
IPHKSPCPRR TIWLSRSQSD IFSRKPPRTV SVLDPYYRPR DGAARTPKVN
410 420 430 440 450
PFSARQDLMG GKIKFFDLPS KSVISLVFDL DAPGPGTMPL ADWQEPLAPP
460 470 480 490 500
IRRWRSLPGS PEFLHQEACP FVGREESLSD GPPPRLSSLK YRVKEIPPFR
510 520 530 540 550
ASALPAAQAH EAMDCSILQE ENGFGSRPQG TSPCPAGASE EMEVEERPAG
560 570
STPATFSTSG IGLQTQGKQD G
Length:571
Mass (Da):63,639
Last modified:December 1, 2001 - v1
Checksum:iD83B0E58321A46FA
GO
Isoform 2 (identifier: Q96S53-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     116-131: Missing.

Show »
Length:555
Mass (Da):61,887
Checksum:iEFA1A38A53B68FD9
GO
Isoform 3 (identifier: Q96S53-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     265-293: Missing.

Show »
Length:542
Mass (Da):60,332
Checksum:iA4BE7D25116F20E1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti455R → C in AAH33085 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04121411G → A in a breast infiltrating ductal carcinoma sample; somatic mutation. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004930116 – 131Missing in isoform 2. 1 PublicationAdd BLAST16
Alternative sequenceiVSP_004931265 – 293Missing in isoform 3. 1 PublicationAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132545 mRNA Translation: CAB41970.1
AB057597 mRNA Translation: BAB62909.1
AK027573 mRNA Translation: BAG51348.1
AL359540 Genomic DNA No translation available.
AL451136 Genomic DNA No translation available.
CH471059 Genomic DNA Translation: EAX06987.1
BC033085 mRNA Translation: AAH33085.1
CCDSiCCDS41323.1 [Q96S53-1]
RefSeqiNP_001307729.1, NM_001320800.1
NP_009101.2, NM_007170.2 [Q96S53-1]
XP_006710350.1, XM_006710287.2 [Q96S53-1]
XP_011538799.1, XM_011540497.1 [Q96S53-1]
UniGeneiHs.591499

Genome annotation databases

EnsembliENST00000372084; ENSP00000361156; ENSG00000070759 [Q96S53-3]
ENST00000372086; ENSP00000361158; ENSG00000070759 [Q96S53-1]
GeneIDi10420
KEGGihsa:10420
UCSCiuc001cns.2 human [Q96S53-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiTESK2_HUMAN
AccessioniPrimary (citable) accession number: Q96S53
Secondary accession number(s): Q5T422
, Q5T423, Q8N520, Q9Y3Q6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: December 1, 2001
Last modified: May 23, 2018
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families

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