Reviewed, UniProtKB/Swiss-Prot Q96S53 (TESK2_HUMAN)

Names and origin

Protein names

Recommended name:
    Dual specificity testis-specific protein kinase 2
    EC=2.7.12.1
Alternative name(s):
    Testicular protein kinase 2

Gene names

Name:

TESK2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	571 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation of exogenous substrates on both serine/threonine and tyrosine residues. Phosphorylates cofilin at 'Ser-3'. May play an important role in spermatogenesis.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity. Manganese By similarity.
Enzyme regulation	Activated by autophosphorylation on Ser-219 By similarity.
Subcellular location	Nucleus. Ref.2
Tissue specificity	Predominantly expressed in testis and prostate. Found predominantly in non-germinal Sertoli cells. Ref.2 Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	actin cytoskeleton organization Inferred from sequence or structural similarity. Source: UniProtKB focal adhesion formation Inferred from sequence or structural similarity. Source: UniProtKB protein amino acid phosphorylation Inferred from sequence or structural similarity. Source: UniProtKB spermatogenesis Ref.1 Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from sequence or structural similarity. Source: UniProtKB protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Experimental confirmation may be lacking for some isoforms.

Isoform 1 (identifier: Q96S53-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96S53-2)

The sequence of this isoform differs from the canonical sequence as follows:
     116-131: Missing.

Isoform 3 (identifier: Q96S53-3)

The sequence of this isoform differs from the canonical sequence as follows:
     265-293: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 571	571	Dual specificity testis-specific protein kinase 2		PRO_0000086749
Regions
	Domain	58 – 313	256	Protein kinase
	Nucleotide binding	64 – 72	9	ATP By similarity
Sites
	Active site	176	1	Proton acceptor By similarity
	Binding site	87	1	ATP By similarity
Amino acid modifications
	Modified residue	219	1	Phosphoserine; by autocatalysis By similarity
Natural variations
	Alternative sequence	116 – 131	16	Missing in isoform 2.		VSP_004930
	Alternative sequence	265 – 293	29	Missing in isoform 3.		VSP_004931
	Natural variant	11	1	G → A in a breast infiltrating ductal carcinoma sample; somatic mutation. Ref.4		VAR_041214
Experimental info
	Sequence conflict	455	1	R → C in AAH33085. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: D83B0E58321A46FA	FASTA	571	63,639
	10 20 30 40 50 60 MDRSKRNSIA GFPPRVERLE EFEGGGGGEG NVSQVGRVWP SSYRALISAF SRLTRLDDFT 70 80 90 100 110 120 CEKIGSGFFS EVFKVRHRAS GQVMALKMNT LSSNRANMLK EVQLMNRLSH PNILRFMGVC 130 140 150 160 170 180 VHQGQLHALT EYINSGNLEQ LLDSNLHLPW TVRVKLAYDI AVGLSYLHFK GIFHRDLTSK 190 200 210 220 230 240 NCLIKRDENG YSAVVADFGL AEKIPDVSMG SEKLAVVGSP FWMAPEVLRD EPYNEKADVF 250 260 270 280 290 300 SYGIILCEII ARIQADPDYL PRTENFGLDY DAFQHMVGDC PPDFLQLTFN CCNMDPKLRP 310 320 330 340 350 360 SFVEIGKTLE EILSRLQEEE QERDRKLQPT ARGLLEKAPG VKRLSSLDDK IPHKSPCPRR 370 380 390 400 410 420 TIWLSRSQSD IFSRKPPRTV SVLDPYYRPR DGAARTPKVN PFSARQDLMG GKIKFFDLPS 430 440 450 460 470 480 KSVISLVFDL DAPGPGTMPL ADWQEPLAPP IRRWRSLPGS PEFLHQEACP FVGREESLSD 490 500 510 520 530 540 GPPPRLSSLK YRVKEIPPFR ASALPAAQAH EAMDCSILQE ENGFGSRPQG TSPCPAGASE 550 560 570 EMEVEERPAG STPATFSTSG IGLQTQGKQD G
	Isoform 2. Checksum: EFA1A38A53B68FD9	FASTA	555	61,887
	10 20 30 40 50 60 MDRSKRNSIA GFPPRVERLE EFEGGGGGEG NVSQVGRVWP SSYRALISAF SRLTRLDDFT 70 80 90 100 110 120 CEKIGSGFFS EVFKVRHRAS GQVMALKMNT LSSNRANMLK EVQLMNRLSH PNILRYINSG 130 140 150 160 170 180 NLEQLLDSNL HLPWTVRVKL AYDIAVGLSY LHFKGIFHRD LTSKNCLIKR DENGYSAVVA 190 200 210 220 230 240 DFGLAEKIPD VSMGSEKLAV VGSPFWMAPE VLRDEPYNEK ADVFSYGIIL CEIIARIQAD 250 260 270 280 290 300 PDYLPRTENF GLDYDAFQHM VGDCPPDFLQ LTFNCCNMDP KLRPSFVEIG KTLEEILSRL 310 320 330 340 350 360 QEEEQERDRK LQPTARGLLE KAPGVKRLSS LDDKIPHKSP CPRRTIWLSR SQSDIFSRKP 370 380 390 400 410 420 PRTVSVLDPY YRPRDGAART PKVNPFSARQ DLMGGKIKFF DLPSKSVISL VFDLDAPGPG 430 440 450 460 470 480 TMPLADWQEP LAPPIRRWRS LPGSPEFLHQ EACPFVGREE SLSDGPPPRL SSLKYRVKEI 490 500 510 520 530 540 PPFRASALPA AQAHEAMDCS ILQEENGFGS RPQGTSPCPA GASEEMEVEE RPAGSTPATF 550 STSGIGLQTQ GKQDG
	Isoform 3. Checksum: A4BE7D25116F20E1	FASTA	542	60,332
	10 20 30 40 50 60 MDRSKRNSIA GFPPRVERLE EFEGGGGGEG NVSQVGRVWP SSYRALISAF SRLTRLDDFT 70 80 90 100 110 120 CEKIGSGFFS EVFKVRHRAS GQVMALKMNT LSSNRANMLK EVQLMNRLSH PNILRFMGVC 130 140 150 160 170 180 VHQGQLHALT EYINSGNLEQ LLDSNLHLPW TVRVKLAYDI AVGLSYLHFK GIFHRDLTSK 190 200 210 220 230 240 NCLIKRDENG YSAVVADFGL AEKIPDVSMG SEKLAVVGSP FWMAPEVLRD EPYNEKADVF 250 260 270 280 290 300 SYGIILCEII ARIQADPDYL PRTEMDPKLR PSFVEIGKTL EEILSRLQEE EQERDRKLQP 310 320 330 340 350 360 TARGLLEKAP GVKRLSSLDD KIPHKSPCPR RTIWLSRSQS DIFSRKPPRT VSVLDPYYRP 370 380 390 400 410 420 RDGAARTPKV NPFSARQDLM GGKIKFFDLP SKSVISLVFD LDAPGPGTMP LADWQEPLAP 430 440 450 460 470 480 PIRRWRSLPG SPEFLHQEAC PFVGREESLS DGPPPRLSSL KYRVKEIPPF RASALPAAQA 490 500 510 520 530 540 HEAMDCSILQ EENGFGSRPQ GTSPCPAGAS EEMEVEERPA GSTPATFSTS GIGLQTQGKQ DG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of TESK2, a novel member of the LIMK/TESK family of protein kinases, predominantly expressed in testis." Rosok O., Pedeutour F., Ree A.H., Aasheim H.-C. Genomics 61:44-54(1999) [PubMed: 10512679] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY. Tissue: Testis.
[2]	"Cofilin phosphorylation and actin reorganization activities of testicular protein kinase 2 and its predominant expression in testicular Sertoli cells." Toshima J., Toshima J.Y., Takeuchi K., Mori R., Mizuno K. J. Biol. Chem. 276:31449-31458(2001) [PubMed: 11418599] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Brain.
[4]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] ALA-11.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ132545 mRNA. Translation: CAB41970.1. AB057597 mRNA. Translation: BAB62909.1. BC033085 mRNA. Translation: AAH33085.1.
IPI	IPI00102677. IPI00217726. IPI00217728.
RefSeq	NP_009101.2.
UniGene	Hs.271353 Hs.591499
3D structure databases
HSSP	HSSP built from PDB template 1FVR based on UniProtKB Q02763.
ModBase	Search...
Protein-protein interaction databases
STRING	Q96S53.
PTM databases
PhosphoSite	Q96S53.
Proteomic databases
PRIDE	Q96S53.
Genome annotation databases
Ensembl	ENST00000341771; ENSP00000343940; ENSG00000070759; Homo sapiens. [Genome view] ENST00000372083; ENSP00000361155; ENSG00000070759; Homo sapiens. [Genome view] ENST00000372084; ENSP00000361156; ENSG00000070759; Homo sapiens. [Genome view] ENST00000372086; ENSP00000361158; ENSG00000070759; Homo sapiens. [Genome view] ENST00000451835; ENSP00000397244; ENSG00000070759; Homo sapiens. [Genome view]
GeneID	10420.
KEGG	hsa:10420.
UCSC	uc001cns.1. human. uc009vxr.1. human.
Organism-specific databases
CTD	10420.
GeneCards	GC01M045582.
H-InvDB	HIX0000530.
HGNC	HGNC:11732. TESK2.
MIM	604746. gene.
PharmGKB	PA36449.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q96S53.
HOVERGEN	Q96S53.
OMA	RRWRSLP.
Enzyme and pathway databases
BRENDA	2.7.10.2. 247. 2.7.12.1. 247.
Gene expression databases
ArrayExpress	Q96S53.
Bgee	Q96S53.
CleanEx	HS_TESK2.
Genevestigator	Q96S53.
GermOnline	ENSG00000070759. Homo sapiens.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR001245. Tyr_pkinase. IPR008266. Tyr_pkinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PRINTS	PR00109. TYRKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	39492.
SOURCE	Search...

Entry information

Entry name

TESK2_HUMAN

Accession

Primary (citable) accession number: Q96S53
Secondary accession number(s): Q8N520, Q9Y3Q6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	December 1, 2001
Last modified:	November 3, 2009
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.