Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

GPI transamidase component PIG-S

Gene

PIGS

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the GPI transamidase complex. Essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates.1 Publication

Pathwayi: glycosylphosphatidylinositol-anchor biosynthesis

This protein is involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis, which is part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

GO - Biological processi

  • attachment of GPI anchor to protein Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

GPI-anchor biosynthesis

Enzyme and pathway databases

BioCyciZFISH:ENSG00000087111-MONOMER.
ReactomeiR-HSA-162791. Attachment of GPI anchor to uPAR.
UniPathwayiUPA00196.

Names & Taxonomyi

Protein namesi
Recommended name:
GPI transamidase component PIG-S
Alternative name(s):
Phosphatidylinositol-glycan biosynthesis class S protein
Gene namesi
Name:PIGS
ORF Names:UNQ1873/PRO4316
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:14937. PIGS.

Subcellular locationi

  • Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 18CytoplasmicSequence analysisAdd BLAST17
Transmembranei19 – 39HelicalSequence analysisAdd BLAST21
Topological domaini40 – 520LumenalSequence analysisAdd BLAST481
Transmembranei521 – 541HelicalSequence analysisAdd BLAST21
Topological domaini542 – 555CytoplasmicSequence analysisAdd BLAST14

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: Reactome
  • GPI-anchor transamidase complex Source: UniProtKB
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000087111.
PharmGKBiPA33301.

Polymorphism and mutation databases

BioMutaiPIGS.
DMDMi21759353.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002186042 – 555GPI transamidase component PIG-SAdd BLAST554

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi267N-linked (GlcNAc...)Sequence analysis1
Glycosylationi370N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ96S52.
MaxQBiQ96S52.
PaxDbiQ96S52.
PeptideAtlasiQ96S52.
PRIDEiQ96S52.

PTM databases

iPTMnetiQ96S52.
PhosphoSitePlusiQ96S52.

Expressioni

Gene expression databases

BgeeiENSG00000087111.
CleanExiHS_PIGS.
ExpressionAtlasiQ96S52. baseline and differential.
GenevisibleiQ96S52. HS.

Organism-specific databases

HPAiHPA018149.

Interactioni

Subunit structurei

Forms a complex with PIGK/GPI8, PIGT, PIGU and GAA1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
KRT40Q6A1623EBI-2908273,EBI-10171697
KRTAP10-8P604103EBI-2908273,EBI-10171774
NOTCH2NLQ7Z3S95EBI-2908273,EBI-945833

Protein-protein interaction databases

BioGridi125082. 35 interactors.
IntActiQ96S52. 29 interactors.
MINTiMINT-4537363.
STRINGi9606.ENSP00000309430.

Structurei

3D structure databases

ProteinModelPortaliQ96S52.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PIGS family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2459. Eukaryota.
ENOG410XR9U. LUCA.
GeneTreeiENSGT00390000017203.
HOGENOMiHOG000067980.
HOVERGENiHBG031822.
InParanoidiQ96S52.
KOiK05291.
OMAiMSYIGPE.
OrthoDBiEOG091G06XK.
PhylomeDBiQ96S52.
TreeFamiTF105857.

Family and domain databases

InterProiIPR019540. PtdIno-glycan_biosynth_class_S.
[Graphical view]
PANTHERiPTHR21072. PTHR21072. 1 hit.
PfamiPF10510. PIG-S. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96S52-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAGAAATH LEVARGKRAA LFFAAVAIVL GLPLWWKTTE TYRASLPYSQ
60 70 80 90 100
ISGLNALQLR LMVPVTVVFT RESVPLDDQE KLPFTVVHER EIPLKYKMKI
110 120 130 140 150
KCRFQKAYRR ALDHEEEALS SGSVQEAEAM LDEPQEQAEG SLTVYVISEH
160 170 180 190 200
SSLLPQDMMS YIGPKRTAVV RGIMHREAFN IIGRRIVQVA QAMSLTEDVL
210 220 230 240 250
AAALADHLPE DKWSAEKRRP LKSSLGYEIT FSLLNPDPKS HDVYWDIEGA
260 270 280 290 300
VRRYVQPFLN ALGAAGNFSV DSQILYYAML GVNPRFDSAS SSYYLDMHSL
310 320 330 340 350
PHVINPVESR LGSSAASLYP VLNFLLYVPE LAHSPLYIQD KDGAPVATNA
360 370 380 390 400
FHSPRWGGIM VYNVDSKTYN ASVLPVRVEV DMVRVMEVFL AQLRLLFGIA
410 420 430 440 450
QPQLPPKCLL SGPTSEGLMT WELDRLLWAR SVENLATATT TLTSLAQLLG
460 470 480 490 500
KISNIVIKDD VASEVYKAVA AVQKSAEELA SGHLASAFVA SQEAVTSSEL
510 520 530 540 550
AFFDPSLLHL LYFPDDQKFA IYIPLFLPMA VPILLSLVKI FLETRKSWRK

PEKTD
Length:555
Mass (Da):61,656
Last modified:January 23, 2007 - v3
Checksum:iA20B323C5475EF4F
GO
Isoform 2 (identifier: Q96S52-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-11: MAAAGAAATHL → MPS

Note: No experimental confirmation available.
Show »
Length:547
Mass (Da):61,005
Checksum:iFE15DAA8222209B8
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_036510159M → I in a breast cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_053582253R → H.Corresponds to variant rs34669811dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0131581 – 11MAAAGAAATHL → MPS in isoform 2. 1 PublicationAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB057723 mRNA. Translation: BAB60853.1.
AY359112 mRNA. Translation: AAQ89470.1.
BC069228 mRNA. Translation: AAH69228.1.
CCDSiCCDS11235.1. [Q96S52-1]
RefSeqiNP_149975.1. NM_033198.3. [Q96S52-1]
UniGeneiHs.462550.

Genome annotation databases

EnsembliENST00000308360; ENSP00000309430; ENSG00000087111. [Q96S52-1]
ENST00000395346; ENSP00000378755; ENSG00000087111. [Q96S52-2]
GeneIDi94005.
KEGGihsa:94005.
UCSCiuc002hbn.3. human. [Q96S52-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB057723 mRNA. Translation: BAB60853.1.
AY359112 mRNA. Translation: AAQ89470.1.
BC069228 mRNA. Translation: AAH69228.1.
CCDSiCCDS11235.1. [Q96S52-1]
RefSeqiNP_149975.1. NM_033198.3. [Q96S52-1]
UniGeneiHs.462550.

3D structure databases

ProteinModelPortaliQ96S52.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125082. 35 interactors.
IntActiQ96S52. 29 interactors.
MINTiMINT-4537363.
STRINGi9606.ENSP00000309430.

PTM databases

iPTMnetiQ96S52.
PhosphoSitePlusiQ96S52.

Polymorphism and mutation databases

BioMutaiPIGS.
DMDMi21759353.

Proteomic databases

EPDiQ96S52.
MaxQBiQ96S52.
PaxDbiQ96S52.
PeptideAtlasiQ96S52.
PRIDEiQ96S52.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000308360; ENSP00000309430; ENSG00000087111. [Q96S52-1]
ENST00000395346; ENSP00000378755; ENSG00000087111. [Q96S52-2]
GeneIDi94005.
KEGGihsa:94005.
UCSCiuc002hbn.3. human. [Q96S52-1]

Organism-specific databases

CTDi94005.
GeneCardsiPIGS.
HGNCiHGNC:14937. PIGS.
HPAiHPA018149.
MIMi610271. gene.
neXtProtiNX_Q96S52.
OpenTargetsiENSG00000087111.
PharmGKBiPA33301.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2459. Eukaryota.
ENOG410XR9U. LUCA.
GeneTreeiENSGT00390000017203.
HOGENOMiHOG000067980.
HOVERGENiHBG031822.
InParanoidiQ96S52.
KOiK05291.
OMAiMSYIGPE.
OrthoDBiEOG091G06XK.
PhylomeDBiQ96S52.
TreeFamiTF105857.

Enzyme and pathway databases

UniPathwayiUPA00196.
BioCyciZFISH:ENSG00000087111-MONOMER.
ReactomeiR-HSA-162791. Attachment of GPI anchor to uPAR.

Miscellaneous databases

ChiTaRSiPIGS. human.
GeneWikiiPIGS_(gene).
GenomeRNAii94005.
PROiQ96S52.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000087111.
CleanExiHS_PIGS.
ExpressionAtlasiQ96S52. baseline and differential.
GenevisibleiQ96S52. HS.

Family and domain databases

InterProiIPR019540. PtdIno-glycan_biosynth_class_S.
[Graphical view]
PANTHERiPTHR21072. PTHR21072. 1 hit.
PfamiPF10510. PIG-S. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPIGS_HUMAN
AccessioniPrimary (citable) accession number: Q96S52
Secondary accession number(s): Q6UVX6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.