Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q96S52

- PIGS_HUMAN

UniProt

Q96S52 - PIGS_HUMAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

GPI transamidase component PIG-S

Gene

PIGS

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the GPI transamidase complex. Essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates.1 Publication

Pathwayi

GO - Molecular functioni

  1. GPI-anchor transamidase activity Source: Ensembl

GO - Biological processi

  1. attachment of GPI anchor to protein Source: UniProtKB
  2. cellular protein metabolic process Source: Reactome
  3. C-terminal protein lipidation Source: Reactome
  4. post-translational protein modification Source: Reactome
Complete GO annotation...

Keywords - Biological processi

GPI-anchor biosynthesis

Enzyme and pathway databases

ReactomeiREACT_1830. Attachment of GPI anchor to uPAR.
UniPathwayiUPA00196.

Names & Taxonomyi

Protein namesi
Recommended name:
GPI transamidase component PIG-S
Alternative name(s):
Phosphatidylinositol-glycan biosynthesis class S protein
Gene namesi
Name:PIGS
ORF Names:UNQ1873/PRO4316
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:14937. PIGS.

Subcellular locationi

Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: Reactome
  2. GPI-anchor transamidase complex Source: UniProtKB
  3. membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33301.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 555554GPI transamidase component PIG-SPRO_0000218604Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi267 – 2671N-linked (GlcNAc...)Sequence Analysis
Glycosylationi370 – 3701N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ96S52.
PaxDbiQ96S52.
PeptideAtlasiQ96S52.
PRIDEiQ96S52.

PTM databases

PhosphoSiteiQ96S52.

Expressioni

Gene expression databases

BgeeiQ96S52.
CleanExiHS_PIGS.
ExpressionAtlasiQ96S52. baseline.
GenevestigatoriQ96S52.

Organism-specific databases

HPAiHPA018149.

Interactioni

Subunit structurei

Forms a complex with PIGK/GPI8, PIGT, PIGU and GAA1.1 Publication

Protein-protein interaction databases

BioGridi125082. 15 interactions.
IntActiQ96S52. 1 interaction.
MINTiMINT-4537363.
STRINGi9606.ENSP00000309430.

Structurei

3D structure databases

ProteinModelPortaliQ96S52.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 1817CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini40 – 520481LumenalSequence AnalysisAdd
BLAST
Topological domaini542 – 55514CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei19 – 3921HelicalSequence AnalysisAdd
BLAST
Transmembranei521 – 54121HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the PIGS family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG319204.
GeneTreeiENSGT00390000017203.
HOGENOMiHOG000067980.
HOVERGENiHBG031822.
InParanoidiQ96S52.
KOiK05291.
OMAiSYIGPER.
OrthoDBiEOG741Z1V.
PhylomeDBiQ96S52.
TreeFamiTF105857.

Family and domain databases

InterProiIPR019540. PtdIno-glycan_biosynth_class_S.
[Graphical view]
PANTHERiPTHR21072. PTHR21072. 1 hit.
PfamiPF10510. PIG-S. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q96S52-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAGAAATH LEVARGKRAA LFFAAVAIVL GLPLWWKTTE TYRASLPYSQ
60 70 80 90 100
ISGLNALQLR LMVPVTVVFT RESVPLDDQE KLPFTVVHER EIPLKYKMKI
110 120 130 140 150
KCRFQKAYRR ALDHEEEALS SGSVQEAEAM LDEPQEQAEG SLTVYVISEH
160 170 180 190 200
SSLLPQDMMS YIGPKRTAVV RGIMHREAFN IIGRRIVQVA QAMSLTEDVL
210 220 230 240 250
AAALADHLPE DKWSAEKRRP LKSSLGYEIT FSLLNPDPKS HDVYWDIEGA
260 270 280 290 300
VRRYVQPFLN ALGAAGNFSV DSQILYYAML GVNPRFDSAS SSYYLDMHSL
310 320 330 340 350
PHVINPVESR LGSSAASLYP VLNFLLYVPE LAHSPLYIQD KDGAPVATNA
360 370 380 390 400
FHSPRWGGIM VYNVDSKTYN ASVLPVRVEV DMVRVMEVFL AQLRLLFGIA
410 420 430 440 450
QPQLPPKCLL SGPTSEGLMT WELDRLLWAR SVENLATATT TLTSLAQLLG
460 470 480 490 500
KISNIVIKDD VASEVYKAVA AVQKSAEELA SGHLASAFVA SQEAVTSSEL
510 520 530 540 550
AFFDPSLLHL LYFPDDQKFA IYIPLFLPMA VPILLSLVKI FLETRKSWRK

PEKTD
Length:555
Mass (Da):61,656
Last modified:January 23, 2007 - v3
Checksum:iA20B323C5475EF4F
GO
Isoform 2 (identifier: Q96S52-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-11: MAAAGAAATHL → MPS

Note: No experimental confirmation available.

Show »
Length:547
Mass (Da):61,005
Checksum:iFE15DAA8222209B8
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti159 – 1591M → I in a breast cancer sample; somatic mutation. 1 Publication
VAR_036510
Natural varianti253 – 2531R → H.
Corresponds to variant rs34669811 [ dbSNP | Ensembl ].
VAR_053582

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1111MAAAGAAATHL → MPS in isoform 2. 1 PublicationVSP_013158Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB057723 mRNA. Translation: BAB60853.1.
AY359112 mRNA. Translation: AAQ89470.1.
BC069228 mRNA. Translation: AAH69228.1.
CCDSiCCDS11235.1. [Q96S52-1]
RefSeqiNP_149975.1. NM_033198.3. [Q96S52-1]
UniGeneiHs.462550.

Genome annotation databases

EnsembliENST00000308360; ENSP00000309430; ENSG00000087111. [Q96S52-1]
ENST00000395346; ENSP00000378755; ENSG00000087111. [Q96S52-2]
GeneIDi94005.
KEGGihsa:94005.
UCSCiuc002hbn.2. human. [Q96S52-2]
uc002hbo.2. human. [Q96S52-1]

Polymorphism databases

DMDMi21759353.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB057723 mRNA. Translation: BAB60853.1 .
AY359112 mRNA. Translation: AAQ89470.1 .
BC069228 mRNA. Translation: AAH69228.1 .
CCDSi CCDS11235.1. [Q96S52-1 ]
RefSeqi NP_149975.1. NM_033198.3. [Q96S52-1 ]
UniGenei Hs.462550.

3D structure databases

ProteinModelPortali Q96S52.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 125082. 15 interactions.
IntActi Q96S52. 1 interaction.
MINTi MINT-4537363.
STRINGi 9606.ENSP00000309430.

PTM databases

PhosphoSitei Q96S52.

Polymorphism databases

DMDMi 21759353.

Proteomic databases

MaxQBi Q96S52.
PaxDbi Q96S52.
PeptideAtlasi Q96S52.
PRIDEi Q96S52.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000308360 ; ENSP00000309430 ; ENSG00000087111 . [Q96S52-1 ]
ENST00000395346 ; ENSP00000378755 ; ENSG00000087111 . [Q96S52-2 ]
GeneIDi 94005.
KEGGi hsa:94005.
UCSCi uc002hbn.2. human. [Q96S52-2 ]
uc002hbo.2. human. [Q96S52-1 ]

Organism-specific databases

CTDi 94005.
GeneCardsi GC17M026880.
HGNCi HGNC:14937. PIGS.
HPAi HPA018149.
MIMi 610271. gene.
neXtProti NX_Q96S52.
PharmGKBi PA33301.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG319204.
GeneTreei ENSGT00390000017203.
HOGENOMi HOG000067980.
HOVERGENi HBG031822.
InParanoidi Q96S52.
KOi K05291.
OMAi SYIGPER.
OrthoDBi EOG741Z1V.
PhylomeDBi Q96S52.
TreeFami TF105857.

Enzyme and pathway databases

UniPathwayi UPA00196 .
Reactomei REACT_1830. Attachment of GPI anchor to uPAR.

Miscellaneous databases

GeneWikii PIGS_(gene).
GenomeRNAii 94005.
NextBioi 78284.
PROi Q96S52.
SOURCEi Search...

Gene expression databases

Bgeei Q96S52.
CleanExi HS_PIGS.
ExpressionAtlasi Q96S52. baseline.
Genevestigatori Q96S52.

Family and domain databases

InterProi IPR019540. PtdIno-glycan_biosynth_class_S.
[Graphical view ]
PANTHERi PTHR21072. PTHR21072. 1 hit.
Pfami PF10510. PIG-S. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "PIG-S and PIG-T, essential for GPI anchor attachment to proteins, form a complex with GAA1 and GPI8."
    Ohishi K., Inoue N., Kinoshita T.
    EMBO J. 20:4088-4098(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 2-21, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, MEMBRANE TOPOLOGY.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Lung.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. Cited for: VARIANT [LARGE SCALE ANALYSIS] ILE-159.

Entry informationi

Entry nameiPIGS_HUMAN
AccessioniPrimary (citable) accession number: Q96S52
Secondary accession number(s): Q6UVX6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 99 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3