Q96S21 (RB40C_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras-related protein Rab-40C

Alternative name(s):
Rar-like protein
Ras-like protein family member 8C
SOCS box-containing protein RAR3

Gene names

Name:	RAB40C
Synonyms:	RARL, RASL8C

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Ref.4
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Component of the probable SCF-like ECS(RAB40C) E3 ubiquitin-protein ligase complex which contains CUL5, RNF7/RBX2, Elongin BC complex and RAB40C. Interacts with CUL5, RNF7, TCEB1 and TCEB2. Ref.4
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential.
Domain	The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes By similarity.
Sequence similarities	Belongs to the small GTPase superfamily. Rab family. Contains 1 SOCS box domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cell membrane Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Palmitate Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein transport Inferred from electronic annotation. Source: InterPro small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 281	281	Ras-related protein Rab-40C		PRO_0000121261
Regions
	Domain	175 – 228	54	SOCS box
	Nucleotide binding	21 – 28	8	GTP By similarity
	Nucleotide binding	69 – 73	5	GTP By similarity
	Nucleotide binding	126 – 129	4	GTP By similarity
Amino acid modifications
	Lipidation	273	1	S-palmitoyl cysteine Potential
	Lipidation	278	1	S-geranylgeranyl cysteine By similarity
Experimental info
	Mutagenesis	212 – 215	4	LPLP → AAAA: Abolishes interaction with RNF7 and CUL5. Ref.4
	Mutagenesis	221 – 222	2	HL → AA: Abolishes interaction with RNF7.

Sequences

	Sequence		Length	Mass (Da)
	Q96S21 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 478F974645AA91EC	FASTA	281	31,304
	10 20 30 40 50 60 MGSQGSPVKS YDYLLKFLLV GDSDVGKGEI LESLQDGAAE SPYAYSNGID YKTTTILLDG 70 80 90 100 110 120 RRVKLELWDT SGQGRFCTIF RSYSRGAQGI LLVYDITNRW SFDGIDRWIK EIDEHAPGVP 130 140 150 160 170 180 RILVGNRLHL AFKRQVPTEQ ARAYAEKNCM TFFEVSPLCN FNVIESFTEL SRIVLMRHGM 190 200 210 220 230 240 EKIWRPNRVF SLQDLCCRAI VSCTPVHLID KLPLPVTIKS HLKSFSMANG MNAVMMHGRS 250 260 270 280 YSLASGAGGG GSKGNSLKRS KSIRPPQSPP QNCSRSNCKI S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence, structure and pathology of the fully annotated terminal 2 Mb of the short arm of human chromosome 16." Daniels R.J., Peden J.F., Lloyd C., Horsley S.W., Clark K., Tufarelli C., Kearney L., Buckle V.J., Doggett N.A., Flint J., Higgs D.R. Hum. Mol. Genet. 10:339-352(2001) [PubMed: 11157797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The sequence and analysis of duplication-rich human chromosome 16." Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A. Nature 432:988-994(2004) [PubMed: 15616553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"VHL-box and SOCS-box domains determine binding specificity for Cul2-Rbx1 and Cul5-Rbx2 modules of ubiquitin ligases." Kamura T., Maenaka K., Kotoshiba S., Matsumoto M., Kohda D., Conaway R.C., Conaway J.W., Nakayama K.I. Genes Dev. 18:3055-3065(2004) [PubMed: 15601820] [Abstract] Cited for: FUNCTION IN AN E3 UBIQUITIN-PROTEIN LIGASE COMPLEX, MASS SPECTROMETRY, INTERACTION WITH CUL5; RNF7; TCEB1 AND TCEB2, MUTAGENESIS OF 212-LEU--PRO-215 AND 221-HIS-LEU-222.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006464 Genomic DNA. Translation: AAK61236.1. CH471112 Genomic DNA. Translation: EAW85791.1. CH471112 Genomic DNA. Translation: EAW85792.1. CH471112 Genomic DNA. Translation: EAW85793.1. CH471112 Genomic DNA. Translation: EAW85794.1. Z84479, Z98883 Genomic DNA. Translation: CAB06484.2. Z98883, Z84479 Genomic DNA. Translation: CAM26443.1.
IPI	IPI00045478.
RefSeq	NP_001166134.1. NM_001172663.1. NP_001166135.1. NM_001172664.1. NP_001166136.1. NM_001172665.1. NP_066991.3. NM_021168.4.
UniGene	Hs.459630.
3D structure databases
ProteinModelPortal	Q96S21.
SMR	Q96S21. Positions 12-178.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96S21. 6 interactions.
MINT	MINT-1419142.
STRING	Q96S21.
Polymorphism databases
DMDM	27734457.
Proteomic databases
PRIDE	Q96S21.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000248139; ENSP00000248139; ENSG00000197562.
GeneID	57799.
KEGG	hsa:57799.
Organism-specific databases
CTD	57799.
GeneCards	GC16P000664.
HGNC	HGNC:18285. RAB40C.
neXtProt	NX_Q96S21.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00600000084072.
HOGENOM	HBG745225.
HOVERGEN	HBG009351.
InParanoid	Q96S21.
OMA	NGMTFFE.
OrthoDB	EOG45QHDR.
PhylomeDB	Q96S21.
Gene expression databases
ArrayExpress	Q96S21.
Bgee	Q96S21.
CleanEx	HS_RAB40C.
Genevestigator	Q96S21.
GermOnline	ENSG00000197562. Homo sapiens.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR003579. Small_GTPase_Rab_type. IPR001496. SOCS_C. [Graphical view]
KO	K07928.
Pfam	PF00071. Ras. 1 hit. PF07525. SOCS_box. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00175. RAB. 1 hit. SM00253. SOCS. 1 hit. SM00969. SOCS_box. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. Small_GTP. 1 hit.
PROSITE	PS51419. RAB. 1 hit. PS50225. SOCS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RB40C_HUMAN

Accession

Primary (citable) accession number: Q96S21
Secondary accession number(s): A2IDE2 , D3DU54, O60795, Q4TT41

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2003
Last sequence update:	December 1, 2001
Last modified:	January 25, 2012
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.