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Q96RU8 (TRIB1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tribbles homolog 1

Short name=TRB-1
Alternative name(s):
G-protein-coupled receptor-induced gene 2 protein
Short name=GIG-2
SKIP1
Gene names
Name:TRIB1
Synonyms:C8FW, GIG2, TRB1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length372 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Interacts with MAPK kinases and regulates activation of MAP kinases. May not display kinase activity. Ref.1

Tissue specificity

Expressed in most human tissues with the highest levels in skeletal muscle, thyroid gland, pancreas, peripheral blood leukocytes, and bone marrow. Ref.1

Domain

The protein kinase domain is predicted to be catalytically inactive.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
Polymorphism
   Molecular functionProtein kinase inhibitor
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processJNK cascade

Inferred from mutant phenotype PubMed 17452330. Source: BHF-UCL

negative regulation of lipopolysaccharide-mediated signaling pathway

Inferred from mutant phenotype PubMed 17452330. Source: BHF-UCL

negative regulation of protein kinase activity

Inferred from mutant phenotype Ref.1. Source: BHF-UCL

negative regulation of sequence-specific DNA binding transcription factor activity

Inferred from mutant phenotype Ref.1. Source: BHF-UCL

negative regulation of smooth muscle cell migration

Inferred from mutant phenotype PubMed 17452330. Source: BHF-UCL

negative regulation of smooth muscle cell proliferation

Inferred from mutant phenotype PubMed 17452330. Source: BHF-UCL

positive regulation of proteasomal ubiquitin-dependent protein catabolic process

Inferred from sequence or structural similarity PubMed 20410507. Source: BHF-UCL

regulation of MAP kinase activity

Inferred from direct assay Ref.1. Source: UniProtKB

response to lipopolysaccharide

Inferred from mutant phenotype PubMed 17452330. Source: BHF-UCL

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 17452330. Source: BHF-UCL

nucleus

Inferred from direct assay PubMed 17452330. Source: BHF-UCL

   Molecular_functionmitogen-activated protein kinase kinase binding

Inferred from direct assay Ref.1PubMed 17452330. Source: BHF-UCL

protein kinase inhibitor activity

Inferred from mutant phenotype Ref.1. Source: BHF-UCL

transcription factor binding

Inferred from physical interaction PubMed 17724128. Source: BHF-UCL

transferase activity, transferring phosphorus-containing groups

Inferred from electronic annotation. Source: InterPro

ubiquitin protein ligase binding

Inferred from sequence or structural similarity PubMed 20410507. Source: BHF-UCL

ubiquitin-protein transferase regulator activity

Inferred from sequence or structural similarity PubMed 20410507. Source: BHF-UCL

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96RU8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96RU8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-166: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 372372Tribbles homolog 1
PRO_0000131859

Regions

Domain1 – 338338Protein kinase

Natural variations

Alternative sequence1 – 166166Missing in isoform 2.
VSP_054894
Natural variant1731S → R. Ref.10
Corresponds to variant rs56285697 [ dbSNP | Ensembl ].
VAR_042364
Natural variant2151T → M. Ref.10
Corresponds to variant rs34349706 [ dbSNP | Ensembl ].
VAR_042365
Natural variant2671V → I. Ref.10
Corresponds to variant rs56056430 [ dbSNP | Ensembl ].
VAR_042366
Natural variant2981R → C. Ref.10
Corresponds to variant rs55953723 [ dbSNP | Ensembl ].
VAR_042367
Natural variant3601E → A. Ref.10
Corresponds to variant rs35454769 [ dbSNP | Ensembl ].
VAR_042368
Natural variant3601E → D. Ref.10
Corresponds to variant rs16900603 [ dbSNP | Ensembl ].
VAR_042369
Natural variant3711F → L in a lung large cell carcinoma sample; somatic mutation. Ref.10
VAR_042370

Experimental info

Sequence conflict149 – 1502VI → SV in CAA04119. Ref.9
Sequence conflict1631D → S Ref.1
Sequence conflict1631D → S Ref.9

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 6, 2005. Version 2.
Checksum: 5F54E50924B1365B

FASTA37241,009
        10         20         30         40         50         60 
MRVGPVRSAM SGASQPRGPA LLFPATRGVP AKRLLDADDA AAVAAKCPRL SECSSPPDYL 

        70         80         90        100        110        120 
SPPGSPCSPQ PPPAAPGAGG GSGSAPGPSR IADYLLLPLA EREHVSRALC IHTGRELRCK 

       130        140        150        160        170        180 
VFPIKHYQDK IRPYIQLPSH SNITGIVEVI LGETKAYVFF EKDFGDMHSY VRSRKRLREE 

       190        200        210        220        230        240 
EAARLFKQIV SAVAHCHQSA IVLGDLKLRK FVFSTEERTQ LRLESLEDTH IMKGEDDALS 

       250        260        270        280        290        300 
DKHGCPAYVS PEILNTTGTY SGKAADVWSL GVMLYTLLVG RYPFHDSDPS ALFSKIRRGQ 

       310        320        330        340        350        360 
FCIPEHISPK ARCLIRSLLR REPSERLTAP EILLHPWFES VLEPGYIDSE IGTSDQIVPE 

       370 
YQEDSDISSF FC 

« Hide

Isoform 2 [UniParc].

Checksum: 0B6CE8BD11BBC895
Show »

FASTA20623,473

References

« Hide 'large scale' references
[1]"Human tribbles, a protein family controlling mitogen-activated protein kinase cascades."
Kiss-Toth E., Bagstaff S.M., Sung H.Y., Jozsa V., Dempsey C., Caunt J.C., Oxley K.M., Wyllie D.H., Polgar T., Harte M., O'Neill L.A.J., Qwarnstrom E.E., Dower S.K.
J. Biol. Chem. 279:42703-42708(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH MAP2K1 AND MAP2K4.
[2]"Identification of a novel nuclear factor Gig2, as an m1-acetylcholine receptor-induced gene."
Mayhaus M., von der Kammer H., Klaudiny J., Albrecht C., Hoffmann B., Nitsch R.M.
Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Kidney.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[4]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Lymph node.
[5]NHLBI resequencing and genotyping service (RS&G)
Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]"DNA sequence and analysis of human chromosome 8."
Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. expand/collapse author list , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Placenta.
[9]"Characterization of a phosphoprotein whose mRNA is regulated by the mitogenic pathways in dog thyroid cells."
Wilkin F., Suarez-Huerta N., Robaye B., Peetermans J., Libert F., Dumont J.E., Maenhaut C.
Eur. J. Biochem. 248:660-668(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 149-372 (ISOFORM 1).
Tissue: Thyroid.
[10]"Patterns of somatic mutation in human cancer genomes."
Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. expand/collapse author list , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS [LARGE SCALE ANALYSIS] ARG-173; MET-215; ILE-267; CYS-298; ALA-360; ASP-360 AND LEU-371.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF250310 mRNA. Translation: AAK58174.1.
AF205437 mRNA. Translation: AAG35663.1.
AK297539 mRNA. Translation: BAG59938.1.
AL832388 mRNA. Translation: CAI46181.1.
FJ515869 Genomic DNA. Translation: ACS13752.1.
AC091114 Genomic DNA. No translation available.
CH471060 Genomic DNA. Translation: EAW92085.1.
BC063292 mRNA. Translation: AAH63292.1.
AJ000480 mRNA. Translation: CAA04119.1.
CCDSCCDS6357.1.
RefSeqNP_001269914.1. NM_001282985.1.
NP_079471.1. NM_025195.3.
XP_006716560.1. XM_006716497.1.
UniGeneHs.444947.

3D structure databases

ProteinModelPortalQ96RU8.
SMRQ96RU8. Positions 90-370.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115516. 4 interactions.
IntActQ96RU8. 4 interactions.
STRING9606.ENSP00000312150.

PTM databases

PhosphoSiteQ96RU8.

Polymorphism databases

DMDM83305929.

Proteomic databases

MaxQBQ96RU8.
PaxDbQ96RU8.
PRIDEQ96RU8.

Protocols and materials databases

DNASU10221.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000311922; ENSP00000312150; ENSG00000173334.
ENST00000520847; ENSP00000429063; ENSG00000173334.
GeneID10221.
KEGGhsa:10221.
UCSCuc003yrx.3. human. [Q96RU8-1]

Organism-specific databases

CTD10221.
GeneCardsGC08P126442.
HGNCHGNC:16891. TRIB1.
HPAHPA053815.
MIM609461. gene.
neXtProtNX_Q96RU8.
PharmGKBPA134963922.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000231872.
HOVERGENHBG067729.
InParanoidQ96RU8.
KOK08814.
OMAAHCHQSA.
OrthoDBEOG7RJPR4.
PhylomeDBQ96RU8.
TreeFamTF329785.

Enzyme and pathway databases

SignaLinkQ96RU8.

Gene expression databases

ArrayExpressQ96RU8.
BgeeQ96RU8.
CleanExHS_TRIB1.
GenevestigatorQ96RU8.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR024105. TRB1.
IPR024104. Tribbles/Ser_Thr_kinase_40.
[Graphical view]
PANTHERPTHR22961. PTHR22961. 1 hit.
PTHR22961:SF10. PTHR22961:SF10. 1 hit.
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTRIB1. human.
GeneWikiTRIB1.
GenomeRNAi10221.
NextBio35473372.
PROQ96RU8.
SOURCESearch...

Entry information

Entry nameTRIB1_HUMAN
AccessionPrimary (citable) accession number: Q96RU8
Secondary accession number(s): B4DMM6 expand/collapse secondary AC list , C5HU08, O15180, Q9H2Y8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: July 9, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM