Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ubiquitin carboxyl-terminal hydrolase 28

Gene

USP28

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus.4 Publications

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei171 – 1711Nucleophile
Active sitei600 – 6001Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: GO_Central
  2. ubiquitin-specific protease activity Source: UniProtKB

GO - Biological processi

  1. cell proliferation Source: UniProtKB
  2. cellular response to DNA damage stimulus Source: UniProtKB
  3. cellular response to UV Source: UniProtKB
  4. DNA damage checkpoint Source: UniProtKB
  5. DNA repair Source: UniProtKB-KW
  6. intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: UniProtKB
  7. proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
  8. protein deubiquitination Source: UniProtKB
  9. regulation of proteasomal protein catabolic process Source: GO_Central
  10. response to ionizing radiation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

DNA damage, DNA repair, Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.054.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 28 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 28
Ubiquitin thioesterase 28
Ubiquitin-specific-processing protease 28
Gene namesi
Name:USP28
Synonyms:KIAA1515
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:12625. USP28.

Subcellular locationi

  1. Nucleusnucleoplasm 1 Publication

GO - Cellular componenti

  1. nucleolus Source: HPA
  2. nucleoplasm Source: UniProtKB
  3. nucleus Source: HPA
  4. protein complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi171 – 1711C → A: Abolishes deubiquitinase activity. 2 Publications

Organism-specific databases

PharmGKBiPA37250.

Polymorphism and mutation databases

BioMutaiUSP28.
DMDMi20140700.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10771077Ubiquitin carboxyl-terminal hydrolase 28PRO_0000080657Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei67 – 671Phosphoserine1 Publication
Modified residuei714 – 7141Phosphoserine1 Publication
Modified residuei1048 – 10481Phosphothreonine1 Publication

Post-translational modificationi

Degradaded upon nickel ion level or hypoxia exposure.
Phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ96RU2.
PaxDbiQ96RU2.
PRIDEiQ96RU2.

PTM databases

PhosphoSiteiQ96RU2.

Expressioni

Inductioni

Down-regulated upon hypoxia.1 Publication

Gene expression databases

BgeeiQ96RU2.
CleanExiHS_USP28.
ExpressionAtlasiQ96RU2. baseline and differential.
GenevestigatoriQ96RU2.

Organism-specific databases

HPAiHPA006778.
HPA006779.

Interactioni

Subunit structurei

Interacts with isoform 1 of FBXW7; following DNA damage, dissociates from FBXW7 leading to degradation of MYC.3 Publications

Protein-protein interaction databases

BioGridi121683. 50 interactions.
IntActiQ96RU2. 7 interactions.
MINTiMINT-8415329.
STRINGi9606.ENSP00000003302.

Structurei

Secondary structure

1
1077
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi23 – 3210Combined sources
Helixi37 – 4610Combined sources
Turni47 – 493Combined sources
Helixi51 – 588Combined sources
Helixi60 – 634Combined sources
Helixi100 – 11112Combined sources
Helixi121 – 1233Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LVANMR-A22-132[»]
ProteinModelPortaliQ96RU2.
SMRiQ96RU2. Positions 22-132.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini97 – 11620UIMCuratedAdd
BLAST
Domaini162 – 650489USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family. USP28 subfamily.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5077.
GeneTreeiENSGT00390000016082.
HOGENOMiHOG000007956.
HOVERGENiHBG056030.
InParanoidiQ96RU2.
KOiK11849.
OMAiCIASTTQ.
OrthoDBiEOG761BSZ.
PhylomeDBiQ96RU2.
TreeFamiTF329035.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR009060. UBA-like.
IPR003903. Ubiquitin-int_motif.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00726. UIM. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96RU2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTAELQQDDA AGAADGHGSS CQMLLNQLRE ITGIQDPSFL HEALKASNGD
60 70 80 90 100
ITQAVSLLTD ERVKEPSQDT VATEPSEVEG SAANKEVLAK VIDLTHDNKD
110 120 130 140 150
DLQAAIALSL LESPKIQADG RDLNRMHEAT SAETKRSKRK RCEVWGENPN
160 170 180 190 200
PNDWRRVDGW PVGLKNVGNT CWFSAVIQSL FQLPEFRRLV LSYSLPQNVL
210 220 230 240 250
ENCRSHTEKR NIMFMQELQY LFALMMGSNR KFVDPSAALD LLKGAFRSSE
260 270 280 290 300
EQQQDVSEFT HKLLDWLEDA FQLAVNVNSP RNKSENPMVQ LFYGTFLTEG
310 320 330 340 350
VREGKPFCNN ETFGQYPLQV NGYRNLDECL EGAMVEGDVE LLPSDHSVKY
360 370 380 390 400
GQERWFTKLP PVLTFELSRF EFNQSLGQPE KIHNKLEFPQ IIYMDRYMYR
410 420 430 440 450
SKELIRNKRE CIRKLKEEIK ILQQKLERYV KYGSGPARFP LPDMLKYVIE
460 470 480 490 500
FASTKPASES CPPESDTHMT LPLSSVHCSV SDQTSKESTS TESSSQDVES
510 520 530 540 550
TFSSPEDSLP KSKPLTSSRS SMEMPSQPAP RTVTDEEINF VKTCLQRWRS
560 570 580 590 600
EIEQDIQDLK TCIASTTQTI EQMYCDPLLR QVPYRLHAVL VHEGQANAGH
610 620 630 640 650
YWAYIYNQPR QSWLKYNDIS VTESSWEEVE RDSYGGLRNV SAYCLMYIND
660 670 680 690 700
KLPYFNAEAA PTESDQMSEV EALSVELKHY IQEDNWRFEQ EVEEWEEEQS
710 720 730 740 750
CKIPQMESST NSSSQDYSTS QEPSVASSHG VRCLSSEHAV IVKEQTAQAI
760 770 780 790 800
ANTARAYEKS GVEAALSEVM LSPAMQGVIL AIAKARQTFD RDGSEAGLIK
810 820 830 840 850
AFHEEYSRLY QLAKETPTSH SDPRLQHVLV YFFQNEAPKR VVERTLLEQF
860 870 880 890 900
ADKNLSYDER SISIMKVAQA KLKEIGPDDM NMEEYKKWHE DYSLFRKVSV
910 920 930 940 950
YLLTGLELYQ KGKYQEALSY LVYAYQSNAA LLMKGPRRGV KESVIALYRR
960 970 980 990 1000
KCLLELNAKA ASLFETNDDH SVTEGINVMN ELIIPCIHLI INNDISKDDL
1010 1020 1030 1040 1050
DAIEVMRNHW CSYLGQDIAE NLQLCLGEFL PRLLDPSAEI IVLKEPPTIR
1060 1070
PNSPYDLCSR FAAVMESIQG VSTVTVK
Length:1,077
Mass (Da):122,491
Last modified:December 1, 2001 - v1
Checksum:i799C758888C67B82
GO
Isoform 2 (identifier: Q96RU2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     769-800: Missing.

Show »
Length:1,045
Mass (Da):119,121
Checksum:i1A10819BCF8CCBC6
GO
Isoform 3 (identifier: Q96RU2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     583-583: P → E
     584-1077: Missing.

Note: No experimental confirmation available.

Show »
Length:583
Mass (Da):66,243
Checksum:iA932AD8856535EBD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti289 – 30315VQLFY…EGVRE → IVIVMSFLKSLSLCL in BAA96039 (Ref. 4) CuratedAdd
BLAST

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei583 – 5831P → E in isoform 3. 1 PublicationVSP_057359
Alternative sequencei584 – 1077494Missing in isoform 3. 1 PublicationVSP_057360Add
BLAST
Alternative sequencei769 – 80032Missing in isoform 2. 1 PublicationVSP_015580Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF266283 mRNA. Translation: AAK58565.1.
EF445045 Genomic DNA. Translation: ACA06098.1.
EF445045 Genomic DNA. Translation: ACA06099.1.
AP001874 Genomic DNA. No translation available.
AP003170 Genomic DNA. No translation available.
KF455523 Genomic DNA. No translation available.
KF459544 Genomic DNA. No translation available.
CH471065 Genomic DNA. Translation: EAW67232.1.
BC065928 mRNA. Translation: AAH65928.1.
AB040948 mRNA. Translation: BAA96039.1.
CCDSiCCDS31680.1. [Q96RU2-1]
RefSeqiNP_065937.1. NM_020886.3. [Q96RU2-1]
XP_005271696.1. XM_005271639.2. [Q96RU2-2]
UniGeneiHs.503891.

Genome annotation databases

EnsembliENST00000003302; ENSP00000003302; ENSG00000048028. [Q96RU2-1]
ENST00000537706; ENSP00000445743; ENSG00000048028. [Q96RU2-3]
GeneIDi57646.
KEGGihsa:57646.
UCSCiuc001poh.3. human. [Q96RU2-1]
uc001poj.3. human.

Polymorphism and mutation databases

BioMutaiUSP28.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF266283 mRNA. Translation: AAK58565.1.
EF445045 Genomic DNA. Translation: ACA06098.1.
EF445045 Genomic DNA. Translation: ACA06099.1.
AP001874 Genomic DNA. No translation available.
AP003170 Genomic DNA. No translation available.
KF455523 Genomic DNA. No translation available.
KF459544 Genomic DNA. No translation available.
CH471065 Genomic DNA. Translation: EAW67232.1.
BC065928 mRNA. Translation: AAH65928.1.
AB040948 mRNA. Translation: BAA96039.1.
CCDSiCCDS31680.1. [Q96RU2-1]
RefSeqiNP_065937.1. NM_020886.3. [Q96RU2-1]
XP_005271696.1. XM_005271639.2. [Q96RU2-2]
UniGeneiHs.503891.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LVANMR-A22-132[»]
ProteinModelPortaliQ96RU2.
SMRiQ96RU2. Positions 22-132.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121683. 50 interactions.
IntActiQ96RU2. 7 interactions.
MINTiMINT-8415329.
STRINGi9606.ENSP00000003302.

Chemistry

ChEMBLiCHEMBL2157853.

Protein family/group databases

MEROPSiC19.054.

PTM databases

PhosphoSiteiQ96RU2.

Polymorphism and mutation databases

BioMutaiUSP28.
DMDMi20140700.

Proteomic databases

MaxQBiQ96RU2.
PaxDbiQ96RU2.
PRIDEiQ96RU2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000003302; ENSP00000003302; ENSG00000048028. [Q96RU2-1]
ENST00000537706; ENSP00000445743; ENSG00000048028. [Q96RU2-3]
GeneIDi57646.
KEGGihsa:57646.
UCSCiuc001poh.3. human. [Q96RU2-1]
uc001poj.3. human.

Organism-specific databases

CTDi57646.
GeneCardsiGC11M113668.
HGNCiHGNC:12625. USP28.
HPAiHPA006778.
HPA006779.
MIMi610748. gene.
neXtProtiNX_Q96RU2.
PharmGKBiPA37250.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5077.
GeneTreeiENSGT00390000016082.
HOGENOMiHOG000007956.
HOVERGENiHBG056030.
InParanoidiQ96RU2.
KOiK11849.
OMAiCIASTTQ.
OrthoDBiEOG761BSZ.
PhylomeDBiQ96RU2.
TreeFamiTF329035.

Miscellaneous databases

ChiTaRSiUSP28. human.
GenomeRNAii57646.
NextBioi64378.
PROiQ96RU2.
SOURCEiSearch...

Gene expression databases

BgeeiQ96RU2.
CleanExiHS_USP28.
ExpressionAtlasiQ96RU2. baseline and differential.
GenevestigatoriQ96RU2.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR009060. UBA-like.
IPR003903. Ubiquitin-int_motif.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00726. UIM. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of alternatively spliced products and tissue-specific isoforms of USP28 and USP25."
    Valero R., Bayes M., Francisca Sanchez-Font M., Gonzalez-Angulo O., Gonzalez-Duarte R., Marfany G.
    Genome Biol. 2:RESEARCH0043.1-RESEARCH0043.10(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. NHLBI resequencing and genotyping service (RS&G)
    Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Eye.
  6. "Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
    DNA Res. 7:143-150(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 289-1077 (ISOFORM 2).
    Tissue: Brain.
  7. "A role for the deubiquitinating enzyme USP28 in control of the DNA-damage response."
    Zhang D., Zaugg K., Mak T.W., Elledge S.J.
    Cell 126:529-542(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TP53BP1, PHOSPHORYLATION AT SER-67 AND SER-714, MUTAGENESIS OF CYS-171.
  8. "Fbw7 and Usp28 regulate myc protein stability in response to DNA damage."
    Popov N., Herold S., Llamazares M., Schulein C., Eilers M.
    Cell Cycle 6:2327-2331(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH FBXW7.
  9. Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH FBXW7, MUTAGENESIS OF CYS-171.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic kidney.
  11. "The Cdc14B-Cdh1-Plk1 axis controls the G2 DNA-damage-response checkpoint."
    Bassermann F., Frescas D., Guardavaccaro D., Busino L., Peschiaroli A., Pagano M.
    Cell 134:256-267(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "Mechanisms of c-myc degradation by nickel compounds and hypoxia."
    Li Q., Kluz T., Sun H., Costa M.
    PLoS ONE 4:E8531-E8531(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEGRADATION, INDUCTION.
  13. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1048, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  15. "NMR solution structure of the N-terminal domain of human USP28."
    Northeast structural genomics consortium (NESG)
    Submitted (JUL-2012) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 22-132.

Entry informationi

Entry nameiUBP28_HUMAN
AccessioniPrimary (citable) accession number: Q96RU2
Secondary accession number(s): B0YJC0
, B0YJC1, Q6NZX9, Q9P213
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: December 1, 2001
Last modified: April 29, 2015
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Peptidase families
    Classification of peptidase families and list of entries
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.