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Q96RS0

- TGS1_HUMAN

UniProt

Q96RS0 - TGS1_HUMAN

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Protein

Trimethylguanosine synthase

Gene

TGS1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m7G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation.4 Publications

Catalytic activityi

S-adenosyl-L-methionine + m7G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(2,7)G(5')pppR-RNA.
S-adenosyl-L-methionine + m(2,7)G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(2,2,7)G(5')pppR-RNA.

Kineticsi

  1. KM=30 µM for m7GDP1 Publication
  2. KM=5 µM for S-adenosyl-L-methionine1 Publication

pH dependencei

Optimum pH is 8.5-9.5.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei719 – 7191S-adenosyl-L-methionineBy similarity
Binding sitei766 – 76617-methylguanosine1 Publication

GO - Molecular functioni

  1. RNA trimethylguanosine synthase activity Source: BHF-UCL

GO - Biological processi

  1. 7-methylguanosine cap hypermethylation Source: BHF-UCL
  2. 7-methylguanosine RNA capping Source: InterPro
  3. cellular lipid metabolic process Source: Reactome
  4. gene expression Source: Reactome
  5. ncRNA metabolic process Source: Reactome
  6. regulation of transcription, DNA-templated Source: UniProtKB-KW
  7. ribonucleoprotein complex biogenesis Source: BHF-UCL
  8. ribonucleoprotein complex import into nucleus Source: BHF-UCL
  9. RNA metabolic process Source: Reactome
  10. small molecule metabolic process Source: Reactome
  11. spliceosomal snRNP assembly Source: Reactome
  12. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiREACT_11066. snRNP Assembly.
REACT_111118. BMAL1:CLOCK,NPAS2 activates circadian gene expression.
REACT_116145. PPARA activates gene expression.
REACT_118659. RORA activates circadian gene expression.
REACT_118713. YAP1- and WWTR1 (TAZ)-stimulated gene expression.
REACT_118789. REV-ERBA represses gene expression.
REACT_147904. Activation of gene expression by SREBF (SREBP).
REACT_19241. Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha).
REACT_200608. Transcriptional activation of mitochondrial biogenesis.
REACT_24941. Circadian Clock.
REACT_27161. Transcriptional regulation of white adipocyte differentiation.

Names & Taxonomyi

Protein namesi
Recommended name:
Trimethylguanosine synthase (EC:2.1.1.-)
Alternative name(s):
CLL-associated antigen KW-2
Cap-specific guanine-N2 methyltransferase
Hepatocellular carcinoma-associated antigen 137
Nuclear receptor coactivator 6-interacting protein
PRIP-interacting protein with methyltransferase motif
Short name:
PIMT
Short name:
PIPMT
Gene namesi
Name:TGS1
Synonyms:HCA137, NCOA6IP, PIMT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 8

Organism-specific databases

HGNCiHGNC:17843. TGS1.

Subcellular locationi

Cytoplasm. NucleusCajal body
Note: A 90 kDa isoform is found in the nucleus while a 55 kDa isoform is found in the cytoplasm and colocalizes with the tubulin network.

GO - Cellular componenti

  1. cytoplasm Source: HPA
  2. cytosol Source: Reactome
  3. extracellular space Source: UniProt
  4. nucleoplasm Source: Reactome
  5. nucleus Source: HPA
  6. small nuclear ribonucleoprotein complex Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi655 – 6551F → A: Loss of catalytic activity. 1 Publication
Mutagenesisi673 – 6731T → A: Decreases catalytic activity to 13 percent of wild type. 1 Publication
Mutagenesisi696 – 6961D → A: Loss of catalytic activity. 1 Publication
Mutagenesisi704 – 7041N → A: Decreases catalytic activity to 5 percent of wild type. 1 Publication
Mutagenesisi719 – 7191D → A: Loss of catalytic activity. 1 Publication
Mutagenesisi731 – 7311N → A: Decreases catalytic activity to 4 percent of wild type. 1 Publication
Mutagenesisi763 – 7631S → A: Decreases catalytic activity to 26 percent of wild type. 2 Publications
Mutagenesisi766 – 7661W → A: Loss of catalytic activity. 2 Publications
Mutagenesisi807 – 8071R → A: Decreases catalytic activity to 6 percent of wild type. 1 Publication
Mutagenesisi808 – 8081N → A: Decreases catalytic activity to 11 percent of wild type. 1 Publication

Organism-specific databases

PharmGKBiPA162405660.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 853853Trimethylguanosine synthasePRO_0000204468Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei55 – 551Phosphoserine2 Publications
Modified residuei60 – 601Phosphothreonine2 Publications
Modified residuei85 – 851Phosphoserine1 Publication
Modified residuei89 – 891Phosphoserine3 Publications
Modified residuei154 – 1541Phosphoserine2 Publications
Modified residuei438 – 4381Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ96RS0.
PaxDbiQ96RS0.
PRIDEiQ96RS0.

PTM databases

PhosphoSiteiQ96RS0.

Expressioni

Tissue specificityi

Ubiquitously expressed. High expression in heart, skeletal muscle, kidney, liver and placenta.1 Publication

Gene expression databases

BgeeiQ96RS0.
CleanExiHS_TGS1.
ExpressionAtlasiQ96RS0. baseline and differential.
GenevestigatoriQ96RS0.

Organism-specific databases

HPAiHPA025024.
HPA029824.

Interactioni

Subunit structurei

May form homooligomers. Interacts with CREBBP/CBP, EED/WAIT1, EP300/P300, NCOA6/PRIP, PPARBP/PBP and SMN.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NOP58Q9Y2X32EBI-949244,EBI-395469

Protein-protein interaction databases

BioGridi125179. 13 interactions.
DIPiDIP-49970N.
IntActiQ96RS0. 7 interactions.
MINTiMINT-2878093.
STRINGi9606.ENSP00000260129.

Structurei

Secondary structure

1
853
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi636 – 6405
Helixi642 – 6443
Helixi645 – 6495
Helixi651 – 6544
Helixi658 – 6603
Helixi666 – 6716
Helixi675 – 68814
Beta strandi692 – 6965
Helixi703 – 7108
Beta strandi714 – 7207
Helixi722 – 73413
Helixi738 – 7403
Beta strandi741 – 7466
Helixi748 – 7514
Helixi752 – 7543
Beta strandi758 – 7625
Helixi769 – 7735
Beta strandi774 – 7774
Turni779 – 7813
Beta strandi782 – 7854
Helixi787 – 79711
Beta strandi801 – 8066
Helixi811 – 8166
Beta strandi824 – 8318
Beta strandi834 – 8429
Helixi843 – 8453

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3EGIX-ray2.21A/B/C/D653-853[»]
3GDHX-ray2.00A/B/C618-853[»]
ProteinModelPortaliQ96RS0.
SMRiQ96RS0. Positions 634-844.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96RS0.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni631 – 846216Sufficient for catalytic activityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi611 – 62414Lys-richAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0500.
GeneTreeiENSGT00390000018056.
HOGENOMiHOG000154561.
HOVERGENiHBG059797.
InParanoidiQ96RS0.
KOiK14292.
OMAiCSRAFVE.
OrthoDBiEOG70CR73.
PhylomeDBiQ96RS0.
TreeFamiTF313065.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR019012. RNA_cap_Gua-N2-MeTrfase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF09445. Methyltransf_15. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

Q96RS0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCCEKWSRVA EMFLFIEERE DCKILCLCSR AFVEDRKLYN LGLKGYYIRD
60 70 80 90 100
SGNNSGDQAT EEEEGGYSCG TAESHDSKGI GLDESELDSE AELMRSMGLP
110 120 130 140 150
LQFGRITAHK DFEVSMNTRN KVKIKKKKHQ KKYLDEIVQE SWRKEYEEDD
160 170 180 190 200
ILASDDPSSI EQYENTRTYE LQSKKDTETE NPPVENTLSP KLEITEKWEK
210 220 230 240 250
YWNEYGGGLL WQSWQEKHPG QALSSEPWNF PDTKEEWEQH YSQLYWYYLE
260 270 280 290 300
QFQYWEAQGW TFDASQSCDT DTYTSKTEAD DKNDEKCMKV DLVSFPSSPI
310 320 330 340 350
MVDNDSSGTS DKDHSEILDG ISNIKLNSEE VTQSQLDSCT SHDGHQQLSE
360 370 380 390 400
VSSKRECPAS GQSEPRNGGT NEESNSSGNT NTDPPAEDSQ KSSGANTSKD
410 420 430 440 450
RPHASGTDGD ESEEDPPEHK PSKLKRSHEL DIDENPASDF DDSGSLLGFK
460 470 480 490 500
YGSGQKYGGI PNFSHRQVRY LEKNVKLKSK YLDMRRQIKM KNKHIFFTKE
510 520 530 540 550
SEKPFFKKSK ILSKVEKFLT WVNKPMDEEA SQESSSHDNV HDASTSSDSE
560 570 580 590 600
EQDMSVKKGD DLLETNNPEP EKCQSVSSAG ELETENYERD SLLATVPDEQ
610 620 630 640 650
DCVTQEVPDS RQAETEAEVK KKKNKKKNKK VNGLPPEIAA VPELAKYWAQ
660 670 680 690 700
RYRLFSRFDD GIKLDREGWF SVTPEKIAEH IAGRVSQSFK CDVVVDAFCG
710 720 730 740 750
VGGNTIQFAL TGMRVIAIDI DPVKIALARN NAEVYGIADK IEFICGDFLL
760 770 780 790 800
LASFLKADVV FLSPPWGGPD YATAETFDIR TMMSPDGFEI FRLSKKITNN
810 820 830 840 850
IVYFLPRNAD IDQVASLAGP GGQVEIEQNF LNNKLKTITA YFGDLIRRPA

SET
Length:853
Mass (Da):96,620
Last modified:January 11, 2011 - v3
Checksum:iFF670AFBE0979443
GO

Sequence cautioni

The sequence AAH11999.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
The sequence BAB15516.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti566 – 5661Missing in AAK27730. (PubMed:11517327)Curated
Sequence conflicti624 – 6241N → T in AAK83025. (PubMed:12097419)Curated
Sequence conflicti628 – 6281N → T in AAK83025. (PubMed:12097419)Curated
Sequence conflicti681 – 6811I → F in AAK27730. (PubMed:11517327)Curated
Sequence conflicti771 – 7711Y → H in AAL99922. (PubMed:12200376)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti16 – 161I → T.3 Publications
Corresponds to variant rs1818 [ dbSNP | Ensembl ].
VAR_024734
Natural varianti160 – 1601I → V.
Corresponds to variant rs3213971 [ dbSNP | Ensembl ].
VAR_024735
Natural varianti299 – 2991P → S.
Corresponds to variant rs11986329 [ dbSNP | Ensembl ].
VAR_056241
Natural varianti511 – 5111I → T.
Corresponds to variant rs10100659 [ dbSNP | Ensembl ].
VAR_024736
Natural varianti576 – 5761V → I.
Corresponds to variant rs16922259 [ dbSNP | Ensembl ].
VAR_024737
Natural varianti595 – 5951T → A.
Corresponds to variant rs10109493 [ dbSNP | Ensembl ].
VAR_056242
Natural varianti754 – 7541F → C.
Corresponds to variant rs7823773 [ dbSNP | Ensembl ].
VAR_024738

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY028423 mRNA. Translation: AAK27730.1.
AF432215 mRNA. Translation: AAL99922.1.
AF286340 mRNA. Translation: AAK83025.1.
AC100817 Genomic DNA. No translation available.
AY534911 mRNA. Translation: AAT02709.1.
BC011999 mRNA. Translation: AAH11999.1. Different initiation.
AK026648 mRNA. Translation: BAB15516.1. Different initiation.
CCDSiCCDS34894.1.
RefSeqiNP_079107.6. NM_024831.6.
UniGeneiHs.335068.

Genome annotation databases

EnsembliENST00000260129; ENSP00000260129; ENSG00000137574.
GeneIDi96764.
KEGGihsa:96764.
UCSCiuc003xsj.4. human.

Polymorphism databases

DMDMi317373500.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY028423 mRNA. Translation: AAK27730.1 .
AF432215 mRNA. Translation: AAL99922.1 .
AF286340 mRNA. Translation: AAK83025.1 .
AC100817 Genomic DNA. No translation available.
AY534911 mRNA. Translation: AAT02709.1 .
BC011999 mRNA. Translation: AAH11999.1 . Different initiation.
AK026648 mRNA. Translation: BAB15516.1 . Different initiation.
CCDSi CCDS34894.1.
RefSeqi NP_079107.6. NM_024831.6.
UniGenei Hs.335068.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3EGI X-ray 2.21 A/B/C/D 653-853 [» ]
3GDH X-ray 2.00 A/B/C 618-853 [» ]
ProteinModelPortali Q96RS0.
SMRi Q96RS0. Positions 634-844.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 125179. 13 interactions.
DIPi DIP-49970N.
IntActi Q96RS0. 7 interactions.
MINTi MINT-2878093.
STRINGi 9606.ENSP00000260129.

PTM databases

PhosphoSitei Q96RS0.

Polymorphism databases

DMDMi 317373500.

Proteomic databases

MaxQBi Q96RS0.
PaxDbi Q96RS0.
PRIDEi Q96RS0.

Protocols and materials databases

DNASUi 96764.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000260129 ; ENSP00000260129 ; ENSG00000137574 .
GeneIDi 96764.
KEGGi hsa:96764.
UCSCi uc003xsj.4. human.

Organism-specific databases

CTDi 96764.
GeneCardsi GC08P056685.
H-InvDB HIX0007515.
HGNCi HGNC:17843. TGS1.
HPAi HPA025024.
HPA029824.
MIMi 606461. gene.
neXtProti NX_Q96RS0.
PharmGKBi PA162405660.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0500.
GeneTreei ENSGT00390000018056.
HOGENOMi HOG000154561.
HOVERGENi HBG059797.
InParanoidi Q96RS0.
KOi K14292.
OMAi CSRAFVE.
OrthoDBi EOG70CR73.
PhylomeDBi Q96RS0.
TreeFami TF313065.

Enzyme and pathway databases

Reactomei REACT_11066. snRNP Assembly.
REACT_111118. BMAL1:CLOCK,NPAS2 activates circadian gene expression.
REACT_116145. PPARA activates gene expression.
REACT_118659. RORA activates circadian gene expression.
REACT_118713. YAP1- and WWTR1 (TAZ)-stimulated gene expression.
REACT_118789. REV-ERBA represses gene expression.
REACT_147904. Activation of gene expression by SREBF (SREBP).
REACT_19241. Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha).
REACT_200608. Transcriptional activation of mitochondrial biogenesis.
REACT_24941. Circadian Clock.
REACT_27161. Transcriptional regulation of white adipocyte differentiation.

Miscellaneous databases

EvolutionaryTracei Q96RS0.
GeneWikii TGS1.
GenomeRNAii 96764.
NextBioi 78561.
PROi Q96RS0.
SOURCEi Search...

Gene expression databases

Bgeei Q96RS0.
CleanExi HS_TGS1.
ExpressionAtlasi Q96RS0. baseline and differential.
Genevestigatori Q96RS0.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR019012. RNA_cap_Gua-N2-MeTrfase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF09445. Methyltransf_15. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of PIMT, a protein with a methyltransferase domain, which interacts with and enhances nuclear receptor coactivator PRIP function."
    Zhu Y.-J., Qi C., Cao W.-Q., Yeldandi A.V., Rao M.S., Reddy J.K.
    Proc. Natl. Acad. Sci. U.S.A. 98:10380-10385(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH NCOA6, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, VARIANT THR-16.
    Tissue: Liver.
  2. "Identification of tumor-associated antigens in chronic lymphocytic leukemia by SEREX."
    Krackhardt A.M., Witzens M., Harig S., Hodi F.S., Zauls A.J., Chessia M., Barrett P., Gribben J.G.
    Blood 100:2123-2131(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT THR-16.
  3. "Large scale identification of human hepatocellular carcinoma-associated antigens by autoantibodies."
    Wang Y., Han K.-J., Pang X.-W., Vaughan H.A., Qu W., Dong X.-Y., Peng J.-R., Zhao H.-T., Rui J.-A., Leng X.-S., Cebon J., Burgess A.W., Chen W.-F.
    J. Immunol. 169:1102-1109(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT THR-16.
  4. "DNA sequence and analysis of human chromosome 8."
    Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
    , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
    Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "SEREX-defined rhabdomyosarcoma antigens."
    Behrends U., Gotz C., Mautner J.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 276-853.
    Tissue: Rhabdomyosarcoma.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 574-853.
    Tissue: Urinary bladder.
  7. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 629-853.
  8. "Mammalian and yeast U3 snoRNPs are matured in specific and related nuclear compartments."
    Verheggen C., Lafontaine D.L.J., Samarsky D., Mouaikel J., Blanchard J.-M., Bordonne R., Bertrand E.
    EMBO J. 21:2736-2745(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  9. "Interaction of PIMT with transcriptional coactivators CBP, p300, and PBP differential role in transcriptional regulation."
    Misra P., Qi C., Yu S., Shah S.H., Cao W.Q., Rao M.S., Thimmapaya B., Zhu Y., Reddy J.K.
    J. Biol. Chem. 277:20011-20019(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CREBBP; EP300 AND PPARBP.
  10. "Different isoforms of PRIP-interacting protein with methyltransferase domain/trimethylguanosine synthase localize to the cytoplasm and nucleus."
    Enuenlue I., Papai G., Cserpan I., Udvardy A., Jeang K.-T., Boros I.
    Biochem. Biophys. Res. Commun. 309:44-51(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH EED, SUBCELLULAR LOCATION.
  11. "Interaction between the small-nuclear-RNA cap hypermethylase and the spinal muscular atrophy protein, survival of motor neuron."
    Mouaikel J., Narayanan U., Verheggen C., Matera A.G., Bertrand E., Tazi J., Bordonne R.
    EMBO Rep. 4:616-622(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SMN.
  12. "Ongoing U snRNP biogenesis is required for the integrity of Cajal bodies."
    Lemm I., Girard C., Kuhn A.N., Watkins N.J., Schneider M., Bordonne R., Luehrmann R.
    Mol. Biol. Cell 17:3221-3231(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. "Genetic and biochemical analysis of yeast and human cap trimethylguanosine synthase: functional overlap of 2,2,7-trimethylguanosine caps, small nuclear ribonucleoprotein components, pre-mRNA splicing factors, and RNA decay pathways."
    Hausmann S., Zheng S., Costanzo M., Brost R.L., Garcin D., Boone C., Shuman S., Schwer B.
    J. Biol. Chem. 283:31706-31718(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF PHE-655; THR-673; ASP-696; ASN-704; ASP-719; ASN-731; SER-763; TRP-766; ARG-807 AND ASN-808.
  14. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-55; THR-60; SER-154 AND SER-438, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  15. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  16. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-55; THR-60; SER-89; SER-154 AND SER-438, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  17. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-85 AND SER-89, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  18. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-89, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  19. "Structure analysis of the conserved methyltransferase domain of human trimethylguanosine synthase TGS1."
    Monecke T., Dickmanns A., Strasser A., Ficner R.
    Acta Crystallogr. D 65:332-338(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.21 ANGSTROMS) OF 653-853.
  20. "Structural basis for m7G-cap hypermethylation of small nuclear, small nucleolar and telomerase RNA by the dimethyltransferase TGS1."
    Monecke T., Dickmanns A., Ficner R.
    Nucleic Acids Res. 37:3865-3877(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 618-853 IN COMPLEX WITH 7-METHYLGUANOSINE AND S-ADENOSYL-L-HOMOCYSTEINE, MUTAGENESIS OF SER-763 AND TRP-766.

Entry informationi

Entry nameiTGS1_HUMAN
AccessioniPrimary (citable) accession number: Q96RS0
Secondary accession number(s): A6NJQ5
, Q5GH23, Q8TDG9, Q96QU3, Q9H5V3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 11, 2011
Last modified: October 29, 2014
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3