Q96RQ9 (OXLA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-amino-acid oxidase
Short name=LAAO
Short name=LAO
EC=1.4.3.2

Alternative name(s):
Interleukin-4-induced protein 1
Short name=IL4-induced protein 1
Protein Fig-1
Short name=hFIG1

Gene names

Name:	IL4I1
Synonyms:	FIG1
ORF Names:	UNQ636/PRO1265

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	567 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Lysosomal L-amino-acid oxidase with highest specific activity with phenylalanine. May play a role in lysosomal antigen processing and presentation By similarity.
Catalytic activity	An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.
Cofactor	FAD By similarity.
Subcellular location	Isoform 1: Lysosome By similarity Ref.2. Isoform 2: Lysosome By similarity Ref.2.
Tissue specificity	Isoform 1 is primarily found in immune tissues. Ref.1
Induction	By IL4/interleukin-4.
Sequence similarities	Belongs to the flavin monoamine oxidase family. FIG1 subfamily.
Sequence caution	The sequence BAB84923.1 differs from that shown. Reason: Erroneous initiation. The sequence CAI54291.1 differs from that shown. Reason: Erroneous initiation. The sequence CAI54292.1 differs from that shown. Reason: Erroneous initiation. The sequence CAI54293.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Lysosome
Coding sequence diversity	Alternative promoter usage Alternative splicing Polymorphism
Domain	Signal
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	extracellular region Traceable author statement. Source: Reactome lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell nuclear membrane Inferred from direct assay. Source: HPA
Molecular_function	L-amino-acid oxidase activity Inferred from experiment. Source: Reactome
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative promoter usage and alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96RQ9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96RQ9-2)

Also known as: IL4I1_2;

The sequence of this isoform differs from the canonical sequence as follows:
     1-5: MAPLA → MPNDDFCPGLTIKAMGAERAPQRQPCT

Note: Uses the promoter of the upstream NUP62 gene and shares the first 2 non-coding exons with NUP62.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Ref.8
	Chain	22 – 567	546	L-amino-acid oxidase		PRO_0000001710
Regions
	Nucleotide binding	115 – 116	2	FAD By similarity
	Nucleotide binding	490 – 493	4	FAD By similarity
Sites
	Binding site	89	1	FAD By similarity
	Binding site	97	1	FAD By similarity
	Binding site	116	1	Substrate By similarity
	Binding site	287	1	FAD; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	398	1	Substrate By similarity
	Binding site	481	1	FAD By similarity
Amino acid modifications
	Glycosylation	54	1	N-linked (GlcNAc...) Potential
	Glycosylation	134	1	N-linked (GlcNAc...) Potential
	Glycosylation	220	1	N-linked (GlcNAc...) Ref.9
	Glycosylation	559	1	N-linked (GlcNAc...) Potential
	Disulfide bond	36 ↔ 199		By similarity
Natural variations
	Alternative sequence	1 – 5	5	MAPLA → MPNDDFCPGLTIKAMGAERA PQRQPCT in isoform 2.		VSP_017173
	Natural variant	501	1	A → S. Corresponds to variant rs2290772 [ dbSNP | Ensembl ].		VAR_048260
Experimental info
	Sequence conflict	24	1	W → R in AAH64378. Ref.7
	Sequence conflict	114	1	A → T in CAI54291. Ref.2
	Sequence conflict	114	1	A → T in CAI54293. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: FFF953CB28D22F19	FASTA	567	62,881
	10 20 30 40 50 60 MAPLALHLLV LVPILLSLVA SQDWKAERSQ DPFEKCMQDP DYEQLLKVVT WGLNRTLKPQ 70 80 90 100 110 120 RVIVVGAGVA GLVAAKVLSD AGHKVTILEA DNRIGGRIFT YRDQNTGWIG ELGAMRMPSS 130 140 150 160 170 180 HRILHKLCQG LGLNLTKFTQ YDKNTWTEVH EVKLRNYVVE KVPEKLGYAL RPQEKGHSPE 190 200 210 220 230 240 DIYQMALNQA LKDLKALGCR KAMKKFERHT LLEYLLGEGN LSRPAVQLLG DVMSEDGFFY 250 260 270 280 290 300 LSFAEALRAH SCLSDRLQYS RIVGGWDLLP RALLSSLSGL VLLNAPVVAM TQGPHDVHVQ 310 320 330 340 350 360 IETSPPARNL KVLKADVVLL TASGPAVKRI TFSPPLPRHM QEALRRLHYV PATKVFLSFR 370 380 390 400 410 420 RPFWREEHIE GGHSNTDRPS RMIFYPPPRE GALLLASYTW SDAAAAFAGL SREEALRLAL 430 440 450 460 470 480 DDVAALHGPV VRQLWDGTGV VKRWAEDQHS QGGFVVQPPA LWQTEKDDWT VPYGRIYFAG 490 500 510 520 530 540 EHTAYPHGWV ETAVKSALRA AIKINSRKGP ASDTASPEGH ASDMEGQGHV HGVASSPSHD 550 560 LAKEEGSHPP VQGQLSLQNT THTRTSH
	Isoform 2 (IL4I1_2) [UniParc]. Checksum: 61FEFA634DF56B49	FASTA	589	65,328
	10 20 30 40 50 60 MPNDDFCPGL TIKAMGAERA PQRQPCTLHL LVLVPILLSL VASQDWKAER SQDPFEKCMQ 70 80 90 100 110 120 DPDYEQLLKV VTWGLNRTLK PQRVIVVGAG VAGLVAAKVL SDAGHKVTIL EADNRIGGRI 130 140 150 160 170 180 FTYRDQNTGW IGELGAMRMP SSHRILHKLC QGLGLNLTKF TQYDKNTWTE VHEVKLRNYV 190 200 210 220 230 240 VEKVPEKLGY ALRPQEKGHS PEDIYQMALN QALKDLKALG CRKAMKKFER HTLLEYLLGE 250 260 270 280 290 300 GNLSRPAVQL LGDVMSEDGF FYLSFAEALR AHSCLSDRLQ YSRIVGGWDL LPRALLSSLS 310 320 330 340 350 360 GLVLLNAPVV AMTQGPHDVH VQIETSPPAR NLKVLKADVV LLTASGPAVK RITFSPPLPR 370 380 390 400 410 420 HMQEALRRLH YVPATKVFLS FRRPFWREEH IEGGHSNTDR PSRMIFYPPP REGALLLASY 430 440 450 460 470 480 TWSDAAAAFA GLSREEALRL ALDDVAALHG PVVRQLWDGT GVVKRWAEDQ HSQGGFVVQP 490 500 510 520 530 540 PALWQTEKDD WTVPYGRIYF AGEHTAYPHG WVETAVKSAL RAAIKINSRK GPASDTASPE 550 560 570 580 GHASDMEGQG HVHGVASSPS HDLAKEEGSH PPVQGQLSLQ NTTHTRTSH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the human homolog of the IL-4 induced gene-1 (Fig1)." Chavan S.S., Tian W., Hsueh K., Jawaheer D., Gregersen P.K., Chu C.C. Biochim. Biophys. Acta 1576:70-80(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]	"Alternative pre-mRNA processing regulates cell-type specific expression of the IL4l1 and NUP62 genes." Wiemann S., Kolb-Kokocinski A., Poustka A. BMC Biol. 3:16-16(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE PROMOTER USAGE, SUBCELLULAR LOCATION.
[3]	"Interleukin-4 induced gene-1 variant (IL4I1_v2) is expressed during spermatogenesis and is decreased in pathologies with hypospermatogenesis." Chu C.C., Reese S.T., George J., Tian W., Yuan L., Millan C., Marmar J.L., Benoff S. Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Testis.
[4]	"The nucleotide sequence of a long cDNA clone isolated from human spleen." Jikuya H., Takano J., Nomura N., Kikuno R., Nagase T., Ohara O. Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Spleen.
[5]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Ovary and PNS.
[8]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 22-36.
[9]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-220, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF293462 mRNA. Translation: AAK73362.1.
AF293463 Genomic DNA. Translation: AAK73363.1.
AJ880386 mRNA. Translation: CAI54291.1. Different initiation.
AJ880387 mRNA. Translation: CAI54292.1. Different initiation.
AJ880388 mRNA. Translation: CAI54293.1. Different initiation.
DQ079587 mRNA. Translation: AAZ32711.1.
DQ079588 mRNA. Translation: AAZ32712.1.
DQ079589 mRNA. Translation: AAZ32713.1.
AK074097 mRNA. Translation: BAB84923.1. Different initiation.
AY358933 mRNA. Translation: AAQ89292.1.
CH471177 Genomic DNA. Translation: EAW52575.1.
BC064378 mRNA. Translation: AAH64378.2.
BC090852 mRNA. Translation: AAH90852.2.

IPI

IPI00045464.
IPI00414928.

RefSeq

NP_001244946.1. NM_001258017.1.
NP_001244947.1. NM_001258018.1.
NP_690863.1. NM_152899.1.
NP_758962.1. NM_172374.2.

UniGene

Hs.574492.
Hs.741750.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2I8H	model	-	@	30-504	[»]

ProteinModelPortal

Q96RQ9.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000342557.

PTM databases

PhosphoSite

Q96RQ9.

Polymorphism databases

DMDM

20138284.

Proteomic databases

PaxDb

Q96RQ9.

PRIDE

Q96RQ9.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000341114; ENSP00000342557; ENSG00000104951.
ENST00000391826; ENSP00000375702; ENSG00000104951.
ENST00000595948; ENSP00000472474; ENSG00000104951.

GeneID

259307.

KEGG

hsa:259307.

UCSC

uc002pqt.1. human.

Organism-specific databases

CTD

259307.

GeneCards

GC19M050392.

HGNC

HGNC:19094. IL4I1.

HPA

CAB020724.
HPA045598.

MIM

609742. gene.

neXtProt

NX_Q96RQ9.

PharmGKB

PA38795.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG1231.

HOGENOM

HOG000088141.

HOVERGEN

HBG005729.

KO

K03334.

OMA

MRFVEVD.

Enzyme and pathway databases

Reactome

REACT_116125. Disease.

Gene expression databases

ArrayExpress

Q96RQ9.

Bgee

Q96RQ9.

Genevestigator

Q96RQ9.

GermOnline

ENSG00000104951. Homo sapiens.

Family and domain databases

InterPro

IPR002937. Amino_oxidase.
IPR001613. Flavin_amine_oxidase.
[Graphical view]

Pfam

PF01593. Amino_oxidase. 1 hit.
[Graphical view]

PRINTS

PR00757. AMINEOXDASEF.

ProtoNet

Search...

Other

ChiTaRS

IL4I1. human.

GenomeRNAi

259307.

NextBio

93149.

SOURCE

Search...

Entry information

Entry name

OXLA_HUMAN

Accession

Primary (citable) accession number: Q96RQ9
Secondary accession number(s): Q1WMJ3 , Q4GZN1, Q4GZN2, Q6P2Q3, Q8TEM5, Q96RQ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.