ID   G3ST4_HUMAN             Reviewed;         486 AA.
AC   Q96RP7; A4D2A8; B4DWL8; D6W5U5; Q8N3P7; Q8WZ17; Q96E33; Q9HA78;
DT   10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   27-MAR-2024, entry version 160.
DE   RecName: Full=Galactose-3-O-sulfotransferase 4;
DE            Short=Gal3ST-4;
DE            EC=2.8.2.-;
DE   AltName: Full=Beta-galactose-3-O-sulfotransferase 4;
DE   AltName: Full=Gal-beta-1,3-GalNAc 3'-sulfotransferase;
GN   Name=GAL3ST4; ORFNames=PP6968;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, AND TISSUE SPECIFICITY.
RC   TISSUE=Testis;
RX   PubMed=11333265; DOI=10.1074/jbc.m101558200;
RA   Seko A., Hara-Kuge S., Yamashita K.;
RT   "Molecular cloning and characterization of a novel human galactose 3-O-
RT   sulfotransferase that transfers sulfate to Galbeta1->3GalNAc residue in O-
RT   glycans.";
RL   J. Biol. Chem. 276:25697-25704(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT VAL-467.
RX   PubMed=15498874; DOI=10.1073/pnas.0404089101;
RA   Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X.,
RA   Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X.,
RA   Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.;
RT   "Large-scale cDNA transfection screening for genes related to cancer
RT   development and progression.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Mammary gland;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Amygdala;
RX   PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA   Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA   Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA   Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA   Wiemann S., Schupp I.;
RT   "The full-ORF clone resource of the German cDNA consortium.";
RL   BMC Genomics 8:399-399(2007).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12853948; DOI=10.1038/nature01782;
RA   Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA   Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K.,
RA   Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A.,
RA   Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H.,
RA   Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A.,
RA   Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P.,
RA   Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M.,
RA   Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S.,
RA   Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R.,
RA   Strowmatt C., Latreille P., Miller N., Johnson D., Murray J.,
RA   Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W.,
RA   Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E.,
RA   Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A.,
RA   Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA   Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E.,
RA   Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A.,
RA   Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A.,
RA   Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R.,
RA   McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H.,
RA   Wilson R.K.;
RT   "The DNA sequence of human chromosome 7.";
RL   Nature 424:157-164(2003).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12690205; DOI=10.1126/science.1083423;
RA   Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K.,
RA   Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R.,
RA   Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A.,
RA   Kanematsu E., Gentles S., Christopoulos C.C., Choufani S., Kwasnicka D.,
RA   Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S.,
RA   Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R.,
RA   Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N.,
RA   Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E.,
RA   Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R.,
RA   Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T.,
RA   Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W.,
RA   Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A.,
RA   Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X.,
RA   Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E.,
RA   Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H.,
RA   Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J.,
RA   Adams M.D., Tsui L.-C.;
RT   "Human chromosome 7: DNA sequence and biology.";
RL   Science 300:767-772(2003).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [8]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT VAL-467.
RC   TISSUE=Placenta;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Catalyzes the transfer of sulfate to beta-1,3-linked
CC       galactose residues in O-linked glycoproteins. Good substrates include
CC       asialofetuin, Gal-beta-1,3-GalNAc and Gal-beta-1,3 (GlcNAc-
CC       beta-1,6)GalNAc. {ECO:0000269|PubMed:11333265}.
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 6-7.;
CC   -!- PATHWAY: Protein modification; carbohydrate sulfation.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane
CC       {ECO:0000305}; Single-pass type II membrane protein {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q96RP7-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q96RP7-2; Sequence=VSP_057006, VSP_057007;
CC   -!- TISSUE SPECIFICITY: Expressed mainly in placenta, thymus, testis,
CC       ovary, spinal cord, trachea and adrenal gland and at low levels in
CC       brain, lung, spleen, prostate, small intestine, colon, stomach thyroid
CC       and lymph node. {ECO:0000269|PubMed:11333265}.
CC   -!- SIMILARITY: Belongs to the galactose-3-O-sulfotransferase family.
CC       {ECO:0000305}.
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DR   EMBL; AF316113; AAK73365.1; -; mRNA.
DR   EMBL; AF289575; AAL55759.1; -; mRNA.
DR   EMBL; AK022178; BAB13977.1; -; mRNA.
DR   EMBL; AK301591; BAG63080.1; -; mRNA.
DR   EMBL; AL833824; CAD38686.2; -; mRNA.
DR   EMBL; AC073842; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH236956; EAL23847.1; -; Genomic_DNA.
DR   EMBL; CH471091; EAW76575.1; -; Genomic_DNA.
DR   EMBL; CH471091; EAW76577.1; -; Genomic_DNA.
DR   EMBL; CH471091; EAW76579.1; -; Genomic_DNA.
DR   EMBL; BC012976; AAH12976.1; -; mRNA.
DR   CCDS; CCDS5688.1; -. [Q96RP7-1]
DR   RefSeq; NP_078913.3; NM_024637.4. [Q96RP7-1]
DR   AlphaFoldDB; Q96RP7; -.
DR   BioGRID; 122811; 3.
DR   IntAct; Q96RP7; 1.
DR   STRING; 9606.ENSP00000353142; -.
DR   GlyCosmos; Q96RP7; 1 site, No reported glycans.
DR   GlyGen; Q96RP7; 1 site.
DR   iPTMnet; Q96RP7; -.
DR   PhosphoSitePlus; Q96RP7; -.
DR   BioMuta; GAL3ST4; -.
DR   DMDM; 47116570; -.
DR   EPD; Q96RP7; -.
DR   jPOST; Q96RP7; -.
DR   MassIVE; Q96RP7; -.
DR   PaxDb; 9606-ENSP00000353142; -.
DR   PeptideAtlas; Q96RP7; -.
DR   ProteomicsDB; 5356; -.
DR   ProteomicsDB; 77998; -. [Q96RP7-1]
DR   Antibodypedia; 30607; 217 antibodies from 24 providers.
DR   DNASU; 79690; -.
DR   Ensembl; ENST00000360039.9; ENSP00000353142.4; ENSG00000197093.11. [Q96RP7-1]
DR   Ensembl; ENST00000413800.5; ENSP00000400451.1; ENSG00000197093.11. [Q96RP7-1]
DR   GeneID; 79690; -.
DR   KEGG; hsa:79690; -.
DR   MANE-Select; ENST00000360039.9; ENSP00000353142.4; NM_024637.5; NP_078913.3.
DR   UCSC; uc003utt.4; human. [Q96RP7-1]
DR   AGR; HGNC:24145; -.
DR   CTD; 79690; -.
DR   DisGeNET; 79690; -.
DR   GeneCards; GAL3ST4; -.
DR   HGNC; HGNC:24145; GAL3ST4.
DR   HPA; ENSG00000197093; Tissue enhanced (skin).
DR   MIM; 608235; gene.
DR   neXtProt; NX_Q96RP7; -.
DR   NIAGADS; ENSG00000197093; -.
DR   OpenTargets; ENSG00000197093; -.
DR   PharmGKB; PA134921067; -.
DR   VEuPathDB; HostDB:ENSG00000197093; -.
DR   eggNOG; ENOG502QSHR; Eukaryota.
DR   GeneTree; ENSGT00950000182923; -.
DR   HOGENOM; CLU_040616_1_2_1; -.
DR   InParanoid; Q96RP7; -.
DR   OMA; RNNQYAR; -.
DR   OrthoDB; 3032344at2759; -.
DR   PhylomeDB; Q96RP7; -.
DR   TreeFam; TF314802; -.
DR   PathwayCommons; Q96RP7; -.
DR   SignaLink; Q96RP7; -.
DR   UniPathway; UPA00353; -.
DR   BioGRID-ORCS; 79690; 18 hits in 1147 CRISPR screens.
DR   GeneWiki; GAL3ST4; -.
DR   GenomeRNAi; 79690; -.
DR   Pharos; Q96RP7; Tdark.
DR   PRO; PR:Q96RP7; -.
DR   Proteomes; UP000005640; Chromosome 7.
DR   RNAct; Q96RP7; Protein.
DR   Bgee; ENSG00000197093; Expressed in parotid gland and 154 other cell types or tissues.
DR   ExpressionAtlas; Q96RP7; baseline and differential.
DR   GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR   GO; GO:0032580; C:Golgi cisterna membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IDA:UniProtKB.
DR   GO; GO:0050656; F:3'-phosphoadenosine 5'-phosphosulfate binding; NAS:UniProtKB.
DR   GO; GO:0050694; F:galactose 3-O-sulfotransferase activity; IDA:UniProtKB.
DR   GO; GO:0001733; F:galactosylceramide sulfotransferase activity; IEA:InterPro.
DR   GO; GO:0050698; F:proteoglycan sulfotransferase activity; NAS:UniProtKB.
DR   GO; GO:0007267; P:cell-cell signaling; NAS:UniProtKB.
DR   GO; GO:0009247; P:glycolipid biosynthetic process; IEA:InterPro.
DR   GO; GO:0009101; P:glycoprotein biosynthetic process; IDA:MGI.
DR   GO; GO:0009100; P:glycoprotein metabolic process; NAS:UniProtKB.
DR   GO; GO:0009311; P:oligosaccharide metabolic process; NAS:UniProtKB.
DR   GO; GO:0030166; P:proteoglycan biosynthetic process; NAS:UniProtKB.
DR   GO; GO:0006790; P:sulfur compound metabolic process; NAS:UniProtKB.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   InterPro; IPR009729; Gal-3-0_sulfotransfrase.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR14647; GALACTOSE-3-O-SULFOTRANSFERASE; 1.
DR   PANTHER; PTHR14647:SF57; GALACTOSE-3-O-SULFOTRANSFERASE 4; 1.
DR   Pfam; PF06990; Gal-3-0_sulfotr; 2.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   Genevisible; Q96RP7; HS.
PE   1: Evidence at protein level;
KW   Alternative splicing; Glycoprotein; Golgi apparatus; Membrane;
KW   Reference proteome; Signal-anchor; Transferase; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..486
FT                   /note="Galactose-3-O-sulfotransferase 4"
FT                   /id="PRO_0000085209"
FT   TOPO_DOM        1..18
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        19..39
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        40..486
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        374
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         1..80
FT                   /note="MGPLSPARTLRLWGPRSLGVALGVFMTIGFALQLLGGPFQRRLPGLQLRQPS
FT                   APSLRPALPSCPPRQRLVFLKTHKSGSS -> MTPRSPTSAFRSCTAVSSCLCPSSWPW
FT                   LHLSVTSPFPVPHGPSLSCQDAAALGTSEPGGGSGSLHDHWLCTPALGRALPE (in
FT                   isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:14702039"
FT                   /id="VSP_057006"
FT   VAR_SEQ         81..142
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:14702039"
FT                   /id="VSP_057007"
FT   VARIANT         353
FT                   /note="R -> Q (in dbSNP:rs3800952)"
FT                   /id="VAR_021989"
FT   VARIANT         467
FT                   /note="A -> V (in dbSNP:rs3823646)"
FT                   /evidence="ECO:0000269|PubMed:15489334,
FT                   ECO:0000269|PubMed:15498874"
FT                   /id="VAR_033736"
FT   CONFLICT        137
FT                   /note="M -> L (in Ref. 3; BAB13977)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        201..204
FT                   /note="RGDH -> VGTT (in Ref. 2; AAL55759)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        441
FT                   /note="S -> R (in Ref. 3; BAB13977)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   486 AA;  54166 MW;  2D731D16B191CC3C CRC64;
     MGPLSPARTL RLWGPRSLGV ALGVFMTIGF ALQLLGGPFQ RRLPGLQLRQ PSAPSLRPAL
     PSCPPRQRLV FLKTHKSGSS SVLSLLHRYG DQHGLRFALP ARYQFGYPKL FQASRVKGYR
     PQGGGTQLPF HILCHHMRFN LKEVLQVMPS DSFFFSIVRD PAALARSAFS YYKSTSSAFR
     KSPSLAAFLA NPRGFYRPGA RGDHYARNLL WFDFGLPFPP EKRAKRGNIH PPRDPNPPQL
     QVLPSGAGPR AQTLNPNALI HPVSTVTDHR SQISSPASFD LGSSSFIQWG LAWLDSVFDL
     VMVAEYFDES LVLLADALCW GLDDVVGFMH NAQAGHKQGL STVSNSGLTA EDRQLTARAR
     AWNNLDWALY VHFNRSLWAR IEKYGQGRLQ TAVAELRARR EALAKHCLVG GEASDPKYIT
     DRRFRPFQFG SAKVLGYILR SGLSPQDQEE CERLATPELQ YKDKLDAKQF PPTVSLPLKT
     SRPLSP
//